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Effects of major OMPs and LPS of Brucella on the control of activation of bone marrow-derived dendritic cells and proliferation of T-lymphocytes in mice | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 3 - شماره پیاپی 88، 2024، صفحه 224-232 اصل مقاله (1.18 M) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.48969.7177 | ||
| نویسندگان | ||
| Z. Xu1؛ Z. Tong1؛ H. Zhang1؛ X. Deng1؛ N. Yang1؛ Z. Wang2؛ C. Chen* 2 | ||
| 1Graduated from School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China | ||
| 2Department of Basic Veterinary Medicine, School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China | ||
| چکیده | ||
| Background: Brucella outer membrane proteins (OMPs) are highly immunogenic, and lipopolysaccharides (LPS) are also considered significant antigens, making them potential candidates for subunit vaccines. Aims: To investigate the effects of Brucella OMPs and LPSs on mouse bone marrow-derived dendritic cells (BMDC) activation and T-lymphocyte proliferation. Methods: BMDC were isolated and cultured in vitro, and subsequently co-cultured with Brucella recombinant proteins (rOMP10, rOMP19, rBP26, rOMP25, and rOMP31), as well as smooth LPS (S-LPS) or rough LPS (R-LPS). The expression of maturation markers on the surface of BMDCs was determined using flow cytometry, while the expression of TLR receptors was determined using RT-PCR. The levels of inflammatory cytokines were measured using iELISA, and the impact on the proliferation of mouse T-lymphocytes was assessed using the MTT method. Results: The impact of LPS on BMDC maturation, TLRs-mediated cytokine secretion, and antigen presentation was found to be limited. In contrast, rOMP10, rOMP19, and rBP26 were observed to promote BMDC maturation, increase the expression of TLR-2 and TLR-4 mRNA, and activate T-lymphocyte proliferation by significantly increasing the expression of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-6, IL-12) and antigen-presenting molecules. However, rOMP25 and rOMP31 did not promote BMDC maturation, inhibited the expression of MHCI and MHCII antigen-presenting molecules, and increased the expression of inflammation-suppressing cytokines (IL-10 and IL-4), resulting in the inhibition of T-lymphocyte proliferation. Conclusion: Brucella OMP10, OMP19, and BP26 play an important role in activating the host immune response, while OMP25 together with OMP31 may play a role in Brucella immune escape. | ||
| کلیدواژهها | ||
| Antigen presentation؛ Brucella OMPs؛ Dendritic cell maturation؛ LPS؛ T-lymphocyte proliferation | ||
| مراجع | ||
|
Alloatti, A; Kotsias, F; Pauwels, AM; Carpier, JM; Jouve, M; Timmerman, E; Pace, L; Vargas, P; Maurin, M; Gehrmann, U; Joannas, L; Vivar, OI; Lennon-Duménil, AM; Savina, A; Gevaert, K; Beyaert, R; Hoffmann, E and Amigorena, S (2015). Toll-like receptor 4 engagement on dendritic cells restrains phago-lysosome fusion and promotes cross-presentation of antigens. Immunity. 43: 1087-1100.
Bin, IY; Bin, PW; Myunghwan, J; Suk, K and Sang, YH (2018). Comparative analysis of immune responses to outer membrane antigens OMP10, OMP19, and OMP28 of Brucella abortus. Jpn. J. Infect. Dis., 71: 197-204.
Boes, M; Bertho, N; Cerny, J; Op den Brouw, M; Kirchhausen, T and Ploegh, H (2003). T cells induce extended class II MHC compartments in dendritic cells in a toll-like receptor-dependent manner. J. Immunol., 171: 4081-4088.
Cabeza-Cabrerizo, M; Cardoso, A; Minutti, CM; Pereira da Costa, M and Reis e Sousa, C (2021). Dendritic cells revisited. Annu. Rev. Immunol., 39: 131-166.
D’Anastasio, R; Staniscia, T; Milia, ML; Manzoli, L and Capasso, L (2010). Origin, evolution and paleo-epidemiology of brucellosis. Epidemiol. Infect., 139: 149-156.
Ducrotoy, MJ; Conde-Álvarez, R; Blasco, JM and Moriyón, I (2016). A review of the basis of the immunological diagnosis of ruminant brucellosis. Vet. Immunol. Immunopathol., 171: 81-102.
Elbehiry, A; Aldubaib, M; Marzouk, E; Abalkhail, A; Almuzaini, AM; Rawway, M; Alghamdi, A; Alqarni, A; Aldawsari, M and Draz, A (2023). The development of diagnostic and vaccine strategies for early detection and control of human brucellosis, particularly in endemic areas. Vaccines (Basel). 11: 654.
Fitzgerald, KA and Kagan, JC (2020). Toll-like receptors and the control of immunity. Cell, 180: 1044-1066.
Ghaznavi Rad, E; Khosravi, K; Zarinfar, N and Mosayebi, G (2017). Reduced IFN-γ production in chronic brucellosis patients. Iran J. Immunol., 14: 215-222.
Heidary, M; Dashtbin, S; Ghanavati, R; Mahdizade Ari, M; Bostanghadiri, N; Darbandi, A; Navidifar, T and Talebi, M (2022). Evaluation of brucellosis vaccines: a comprehensive review. Front. Vet. Sci., 9: 925773.
Hongxing, Z; Chuangfu, C; Yuanzhi, W and Liyan, T (2009). Cloning and prokaryotic expression of OMP31 gene from Brucella M5-90. J. Shihezi Univ. (Nat. Sci.), 27: 593-596.
Hou, H; Liu, X and Peng, Q (2019). The advances in brucellosis vaccines. Vaccine. 37: 3981-3988.
Inaba, K; Inaba, M; Romani, N; Aya, H; Deguchi, M; Ikehara, S; Muramatsu, S and Steinman, RM (1992). Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/ macrophage colony-stimulating factor. J. Exp. Med., 176: 1693-1702.
Jianxin, L (2004). Cloning and construction of protocayronic expression vector and its expression of OMP25 gene for Xinjiang sheep Brucella Ovis. MSc Dissertation, School of Shihezi University. PP: 1-48.
Kim, DG; Simborio, HLT; Reyes, AWB; Min, W and Kim, S (2013a). The key roles of toll-like receptor (TLR) for intracellular survival of Brucella. Prev. Vet. Med., 37: 185-192.
Kim, DH; Son, BG; Lim, JJ; Lee, JJ; Kim, DG; Lee, HJ; Min, W; Rhee, MH; Kim, KD; Chang, HH and Kim, S (2013b). The role of a Brucella abortus lipoprotein in intracellular replication and pathogenicity in experimentally infected mice. Microb. Pathog., 54: 34-39.
Langhorne, J; Albano, FR; Hensmann, M; Sanni, L; Cadman, E; Voisine, C and Sponaas, AM (2004). Dendritic cells, pro-inflammatory responses, and antigen presentation in a rodent malaria infection. Immunol. Rev., 201: 35-47.
Liyan, T; Chuangfu, C; Zhiyuan, W; Hui, Z and Hongxin, Z (2009). Expression of Brucella bp26 gene and BP26 protein used as the diagnostic antigen in indirect ELISA. J. Shihezi Univ. (Nat. Sci.), 027: 437-440.
ManLi, Z (2020). Preparation of Brucella 16M LPS monoclonal antibody and establishment of fluorescence polarised antibody detection method. MSc Dissertation, School of Ningxia University. PP: 1-47.
Nagai, Y; Garrett, KP; Ohta, S; Bahrun, U; Kouro, T; Akira, S; Takatsu, K and Kincade, PW (2006). Toll-like receptors on hematopoietic progenitor cells stimulate innate immune system replenishment. Immunity. 24: 801-812.
Ningning, Y; Zhen, W; Yajun, Y; Qian, Z; Shengnan, S; Huan, Z; Chuangfu, C and Minjun, L (2020). Bioinformatics analysis, cloning and expression of OMP19 gene of Brucella and preliminary establishment of ELISA method. Chin. Anim. Husb. Vet. Med., 47: 3078-3087.
Oliveira, SC; de Oliveira, FS; Macedo, GC; de Almeida, LA and Carvalho, NB (2008). The role of innate immune receptors in the control of Brucella abortus infection: Toll-like receptors and beyond. Microbes Infect., 10: 1005-1009.
Pan, P; Tian, Z; Jianping, G; Fengbo, Z; Huizheng, H and Qianjun, P (2019). Change of IFN-γ, TNF-α, IL-6, IL-4 and IL-2 in pain score of spondylitis and ar-thritis caused by Brucella. Chin. J. Immunol., 35: 1880-1886.
Santiago-Schwarz, F; Tucci, J and Carsons, SE (1996). Endogenously produced interleukin 6 is an accessory cytokine for dendritic cell hematopoiesis. Stem Cells. 14: 225-231.
Shojaei, M; Tahmoorespur, M; Soltani, M and Sekhavati, MH (2018). Immunogenicity evaluation of plasmids encoding Brucella melitensis OMP25 and OMP31 antigens in BALB/c mice. Iran J. Basic Med. Sci., 21: 957-964.
Spörri, R and Sousa, CRE (2005). Inflammatory mediators are insufficient for full dendritic cell activation and promote expansion of CD4+ T cell populations lacking helper function. Nat. Immunol., 6: 163-170.
Tiansen, L (2017). Screening, identification and function study on the protein in host cells interacting with Brucella OMP10 protein. Ph.D. Dissertation, School of Shihezi University. PP: 1-139. Vassen, V; Valotteau, C; Feuillie, C; Formosa-Dague, C; Dufrêne, YF and De Bolle, X (2019). Localized incorporation of outer membrane components in the pathogen Brucella abortus. EMBO J., 38: e100323.
Vitry, MA; De Trez, C; Goriely, S; Dumoutier, L; Akira, S; Ryffel, B; Carlier, Y; Letesson, JJ and Muraille, E (2012). Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice. Infect. Immun., 80: 4271-4280.
Waithman, J; Allan, RS; Kosaka, H; Azukizawa, H; Shortman, K; Lutz, MB; Heath, WR; Carbone, FR and Belz, GT (2007). Skin-derived dendritic cells can mediate deletional tolerance of class I-restricted self-reactive T cells. J. Immunol., 179: 4535-4541.
Watts, C; West, MA and Zaru, R (2010). TLR signalling regulated antigen presentation in dendritic cells. Curr. Opin. Immunol., 22: 124-130.
Weihua, L and Li, T (2011). Mediation of toll-like receptors on dendritic cells. Chin. J. Microecol., 23: 1037-1039.
Wohlleber, D; Kashkar, H; Gärtner, K; Frings, MK; Odenthal, M; Hegenbarth, S; Börner, C; Arnold, B; Hämmerling, G; Nieswandt, B; van Rooijen, N; Limmer, A; Cederbrant, K; Heikenwalder, M; Pasparakis, M; Protzer, U; Dienes, HP; Kurts, C; Krönke, M and Knolle, PA (2012). TNF-induced target cell killing by CTL activated through cross-presentation. Cell Rep., 2: 478-487. | ||
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