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Lentil (lens culinaris Medik.) genotype × PGR (Plant growth regulator) interaction and heritability of some traits under in vitro conditions | ||
| Iran Agricultural Research | ||
| مقاله 2، دوره 42، شماره 2، بهمن 2023، صفحه 4-13 اصل مقاله (467.01 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22099/iar.2024.48842.1563 | ||
| نویسندگان | ||
| Hajar ََAshoordan1؛ Masoud Dehdari* 1؛ Asad Masoumiasl1؛ Rahmatollah Karimizadeh2 | ||
| 1Department of Agronomy and Plant breeding, Faculty of Agriculture, Yasouj University, Yasouj, I. R. Iran. | ||
| 2Dryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Gachsaran, I. R. Iran. | ||
| چکیده | ||
| Plant breeders are constantly evaluating genetic diversity resources to lay a foundation for crop improvement. Tissue culture techniques have been applied successfully in many species to generate genetic variation in plants. Researches on lentil genetic diversity and parameter estimation under tissue culture conditions are relatively limited. Therefore, this research was carried out in three separate experiments for genetic parameters estimation and to understand the response of 14 lentil genotypes to callus induction using kinetin (Kin) and 2,4-dichlorophenoxyacetic acid (2,4-D) growth regulators; shoot production using thidiazuron (TDZ), 2-Isopentyladenine (2iP), benzylamino purine (BAP), and Kin growth regulators; and rooting the plants using NAA growth regulator. Results showed that the best treatments were 1 mg/L Kin and 2 mg/L 2,4-D for callus induction, 3 mg/L BAP, 4 mg/L Kin, and 2 mg/L TDZ for shoot regeneration, and 3 mg/L NAA for rooting. The 09S 83259-14ILL6994/ILL5480 and FLIP2010-40L-10770-ILL8119/ILL7686 genotypes were the best in term of callus, shoot, and root induction. The broad sense heritability of most of the traits was high demonstrating lower contribution of environmental factors in phenotypic variances. Overall, large diversity identified among genotypes in vitro conditions and high heritability estimates indicated that selection based on the measured traits including callus, shoot, and root induction will be efficient in tissue culture conditions. | ||
| کلیدواژهها | ||
| Genetic parameters؛ Heritability؛ Plant growth regulators؛ Regeneration | ||
| مراجع | ||
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