اخبار و اعلانات

 آمار

تعداد نشریات24
تعداد شماره‌ها903
تعداد مقالات8,081
تعداد مشاهده مقاله15,203,521
تعداد دریافت فایل اصل مقاله12,847,852
Keshavarzi, Fatemeh, Salehi, Mohammad Saied, Pandamooz, Sareh, Zare, Razieh, Zamani, Mozhdeh, Mostafavipour, Zohreh, Mokarram, Pooneh. (1403). Valproic acid and/or rapamycin preconditioning protects hair follicle stem cells from oxygen glucose serum deprivation-induced oxidative injury via activating Nrf2 pathway. , 13(3), 103-116. doi: 10.22099/mbrc.2024.49302.1922
Fatemeh Keshavarzi; Mohammad Saied Salehi; Sareh Pandamooz; Razieh Zare; Mozhdeh Zamani; Zohreh Mostafavipour; Pooneh Mokarram. "Valproic acid and/or rapamycin preconditioning protects hair follicle stem cells from oxygen glucose serum deprivation-induced oxidative injury via activating Nrf2 pathway". , 13, 3, 1403, 103-116. doi: 10.22099/mbrc.2024.49302.1922
Keshavarzi, Fatemeh, Salehi, Mohammad Saied, Pandamooz, Sareh, Zare, Razieh, Zamani, Mozhdeh, Mostafavipour, Zohreh, Mokarram, Pooneh. (1403). 'Valproic acid and/or rapamycin preconditioning protects hair follicle stem cells from oxygen glucose serum deprivation-induced oxidative injury via activating Nrf2 pathway', , 13(3), pp. 103-116. doi: 10.22099/mbrc.2024.49302.1922
Keshavarzi, Fatemeh, Salehi, Mohammad Saied, Pandamooz, Sareh, Zare, Razieh, Zamani, Mozhdeh, Mostafavipour, Zohreh, Mokarram, Pooneh. Valproic acid and/or rapamycin preconditioning protects hair follicle stem cells from oxygen glucose serum deprivation-induced oxidative injury via activating Nrf2 pathway. , 1403; 13(3): 103-116. doi: 10.22099/mbrc.2024.49302.1922

Valproic acid and/or rapamycin preconditioning protects hair follicle stem cells from oxygen glucose serum deprivation-induced oxidative injury via activating Nrf2 pathway

Molecular Biology Research Communications
دوره 13، شماره 3، 2024، صفحه 103-116    اصل مقاله (1.08 M)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2024.49302.1922
نویسندگان
Fatemeh Keshavarzi1؛ Mohammad Saied Salehi2؛ Sareh Pandamooz3؛ Razieh Zare1؛ Mozhdeh Zamani4؛ Zohreh Mostafavipour* 5؛ Pooneh Mokarram* 4
1Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4Autophagy Research Center, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5Maternal-Fetal Medicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Among leading causes of the ischemic stroke pathogenesis, oxidative stress strongly declines rate of stem cell engraftment at the injury site, and disables stem cell-based therapy as a key treatment for ischemia stroke. To overcome this therapeutic limitation, preconditioning has been represented a possible approach to augment the adaptation and viability of stem cells to oxidative stress. Here, we illustrated protective impacts of valproic acid (VPA) and/or rapamycin (RAPA) preconditioning unto oxygen glucose and serum deprivation (OGSD)-stimulated cell damage in hair follicle-derived stem cells (HFSCs) and surveyed the plausible inducement mechanisms. OGSD, as an in vitro cell injury model, was established and HFSCs viability was observed using MTT assay after VPA, RAPA, and VPA-RAPA preconditioning under OGSD. ROS and MDA production was assessed to reflect oxidative stress. Real-time PCR and western blotting were employed to investigate Nrf2 expression. The activity of Nrf2-related antioxidant enzymes including NQO1, GPx and GSH level were examined. VEGF and BDNF mRNA expression levels were analyzed. Our results showed that VPA and/or RAPA preconditioning ameliorated OGSD-induced decline in HFSCs viability. In addition, they considerably prohibited ROS and MDA generation in the OGSD-treated HFSCs. Furthermore, VPA and/or RAPA preconditioning stimulated Nrf2 nuclear repositioning and NQO1 and GPx activity and GSH amount, as well as expression of paracrine factors VEGF and BDNF in OGSD-treated HFSCs. Thus, the protective effects afforded by VPA and/or RAPA preconditioning, which involved Nrf2-modulated oxidant stress and regulation of VEGF and BDNF expression, display a simple strategy to augment cell-transplantation efficiency for ischemic stroke.
کلیدواژه‌ها
Ischemic stroke؛ Oxidative stress؛ Nrf2؛ Valproic acid؛ Rapamycin؛ Hair follicle stem cell
مراجع
  1. Pandamooz S, Jurek B, Dianatpour M, Haerteis S, Limm K, Oefner PJ, Dargahi L, Borhani-Haghighi A, Miyan JA, Salehi MS. The beneficial effects of chick embryo extract preconditioning on hair follicle stem cells: A promising strategy to generate Schwann cells. Cell Prolif 2023;56: e13397.
  2. Chen CL, Huang WY, Wang EHC, Tai KY, Lin SJ. Functional complexity of hair follicle stem cell niche and therapeutic targeting of niche dysfunction for hair regeneration. J Biomed Sci 2020;27:43.
  3. Perera SN, Kerosuo L. On the road again: Establishment and maintenance of stemness in the neural crest from embryo to adulthood. Stem Cells 2021;39:7-25.
  4. Sieber-Blum M, Hu Y. Epidermal neural crest stem cells (EPI-NCSC) and pluripotency. Stem Cell Rev 2008;4:256-260.
  5. Hu YF, Zhang ZJ, Sieber Blum M. An epidermal neural crest stem cell (EPI‐NCSC) molecular signature. Stem Cells 2006;24:2692-2702.
  6. Achilleos A, Trainor PA. Neural crest stem cells: discovery, properties and potential for therapy. Cell Res 2012;22:288-304.
  7. Salehi MS, Pandamooz S, Safari A, Jurek B, Tamadon A, Namavar MR, Dianatpour M, Dargahi L, Azarpira N, Fattahi S, Shid Moosavi SM, Keshavarz S, Khodabandeh Z, Zare S, Nazari S, Heidari M, Izadi S, Poursadeghfard M, Borhani-Haghighi A. Epidermal neural crest stem cell transplantation as a promising therapeutic strategy for ischemic stroke. CNS Neurosci Ther 2020;26:670-681.
  8. Hicks AU, Lappalainen RS, Narkilahti S, Suuronen R, Corbett D, Sivenius J, Hovatta O, Jolkkonen J. Transplantation of human embryonic stem cell‐derived neural precursor cells and enriched environment after cortical stroke in rats: Cell survival and functional recovery. Eur J Neurosci 2009;29:562-574.
  9. Nakagomi N, Nakagomi T, Kubo S, Nakano Doi A, Saino O, Takata M, Yoshikawa H, Stern DM, Matsuyama T, Taguchi A. Endothelial cells support survival, proliferation, and neuronal differentiation of transplanted adult ischemia-induced neural stem/progenitor cells after cerebral infarction. Stem Cells 2009;27:2185-2195.
  10. Zhu W, Chen J, Cong X, Hu S, Chen X. Hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells. Stem Cells 2006;24:416-425.
  11. Rodrigues M, Turner O, Stolz D, Griffith LG, Wells A. Production of reactive oxygen species by multipotent stromal cells/mesenchymal stem cells upon exposure to fas ligand. Cell Transplant 2012;21:2171-2187.
  12. Radermacher KA, Wingler K, Langhauser F, Altenhofer S, Kleikers P, Hermans JJR, De Angelis MH, Kleinschnitz C, Schmidt HHHW. Neuroprotection after stroke by targeting NOX4 as a source of oxidative stress. Antioxid Redox Signal 2013;18:1418-1427.
  13. Mostafavi-Pour Z, Ramezani F, Keshavarzi, Samadi N. The role of quercetin and vitamin C in Nrf2‑dependent oxidative stress production in breast cancer cells. Oncol Lett 2017; 13:1965-1973.
  14. Chen HH, Chen YT, Huang YW, Tsai HJ, Kuo CC. 4-Ketopinoresinol, a novel naturally occurring ARE activator, induces the Nrf2/HO-1 axis and protects against oxidative stress-induced cell injury via activation of PI3K/AKT signaling. Free Radic Biol Med 2012;52: 1054-1066.
  15. Abbasi A, Mostafavi-Pour Z, Amiri A, Keshavarzi F, Nejabat N, Ramezani F, Sardarian A, Zal F. Chemoprevention of prostate cancer cells by vitamin C plus quercetin: role of Nrf2 in inducing oxidative stress. Nutr Cancer 2021;73:2003-2013.
  16. Sies H, Berndt C, Jones DP. Oxidative stress. Annu Rev Biochem 2017;86:715-748.
  17. Mohammadzadeh M, Halabian R, Gharehbaghian A, Amirizadeh N, Jahanian-Najafabadi A, Mohammadi Roushandeh A, Habibi Roudkenar M. Nrf-2 overexpression in mesenchymal stem cells reduces oxidative stress-induced apoptosis and cytotoxicity. Cell Stress Chaperones 2012;17:553-565.
  18. Garrido-Pascual P, Alonso-Varona A, Castro B, Buron M, Palomares T. H2O2-preconditioned human adipose-derived stem cells (HC016) increase their resistance to oxidative stress by overexpressing Nrf2 and bioenergetic adaptation. Stem Cell Res Ther 2020;11:335.
  19. Sun Z, Chin YE, Zhang DD. Acetylation of Nrf2 by p300/CBP augments promoter-specific DNA binding of Nrf2 during the antioxidant response. Mol Cell Biol 2009;29:2658-2672.
  20. Rajendran P, Dashwood WM, Li L, Kang Y, Kim E, Johnson G, Fischer KA, Löhr CV, Williams DE, Ho E, Yamamoto M, Lieberman DA, Dashwood RH. Nrf2 status affects tumor growth, HDAC3 gene promoter associations, and the response to sulforaphane in the colon. Clin Epigenetics 2015;7:102
  21. Chen X, Wang H, Zhou M, Li X, Fang Z, Gao H, Li Y, Hu W. Valproic acid attenuates traumatic brain injury-induced inflammation in vivo: involvement of autophagy and the Nrf2/ARE signaling pathway. Front Mol Neurosci 2018;11:117.
  22. Wang Z, Tsai LK, Munasinghe J, Leng Y, Fessler EB, Chibane F, Leeds P, Chuang DM. Chronic valproate treatment enhances postischemic angiogenesis and promotes functional recovery in a rat model of ischemic stroke. Stroke 2012;43:2430-2436.
  23. Qian YR, Lee MJ, Hwang S, Kook JH, Kim JK, Bae CS. Neuroprotection by valproic Acid in mouse models of permanent and transient focal cerebral ischemia. Korean J Physiol Pharmacol 2010;14:435-440.
  24. Sakata H, Niizuma K, Yoshioka H, Kim GS, Jung JE, Katsu M, Narasimhan P, Maier CM, Nishiyama Y, Chan PH. Minocycline-preconditioned neural stem cells enhance neuroprotection after ischemic stroke in rats. J Neurosci 2012;32:3462-3473.
  25. Ya BL, Liu Q, Li HF, Cheng HJ, Yu T, Chen L, Wang Y, Yuan LL, Li WJ, Liu WY, Bai B. Uric acid protects against focal cerebral ischemia/reperfusion-induced oxidative stress via activating Nrf2 and regulating neurotrophic factor expression. Oxid Med Cell Longev 2018; 2018:6069150.
  26. Ishii, T., E. Warabi, and G.E. Mann, Circadian control of BDNF-mediated Nrf2 activation in astrocytes protects dopaminergic neurons from ferroptosis. Free Radical Biol Med 2019; 133:169-178.
  27. Huang Y, Mao Y, Li H, Shen G, Nan G. Knockdown of Nrf2 inhibits angiogenesis by downregulating VEGF expression through PI3K/Akt signaling pathway in cerebral microvascular endothelial cells under hypoxic conditions. Biochem Cell Biol 2018;96:475-482.
  28. Kweider N, Fragoulis A, Rosen C, Pecks U, Rath W, Pufe T, Wruck CJ. Interplay between vascular endothelial growth factor (VEGF) and nuclear factor erythroid 2-related factor-2 (Nrf2): implications for preeclampsia. J Biol Chem 2011;286:42863-42872.
  29. Calap-Quintana P, Soriano S, Llorens JV, Al-Ramahi I, Botas J, Molto MD, Martinez-Sebastian MJ. TORC1 inhibition by rapamycin promotes antioxidant defences in a Drosophila model of Friedreich’s ataxia. PLoS One 2015;10:e0132376.
  30. Tai W, Deng S, Wu W, Li Z, Lei W, Wang Y, Vongphouttha C, Zhang T, Dong Z. Rapamycin attenuates the paraquat‐induced pulmonary fibrosis through activating Nrf2 pathway. J Cell Physiol 2020;235:1759-1768.
  31. Clewes O, Narytnyk A, Gillinder KR, Loughney AD, Murdoch AP, Sieber-Blum M. Human epidermal neural crest stem cells (hEPI-NCSC)--characterization and directed differentiation into osteocytes and melanocytes. Stem Cell Rev Rep 2011;7:799-814.
  32. Sieber‐Blum M, Grim M, Hu YF, Szeder V. Pluripotent neural crest stem cells in the adult hair follicle. Dev Dyn 2004;231:258-269.
  33. Vasyliev RG, Gubar OS, Gordiienko IM, Litvinova LS, Rodnichenko AE, Shupletsova VV, Zlatska AV, Yurova KA, Todosenko NM, Khadzhynova VE, Shulha MV, Novikova SN, Zubov DO. Comparative analysis of biological properties of large-scale expanded adult neural crest-derived stem cells isolated from human hair follicle and skin dermis. Stem Cells Int 2019;2019:9640790.
  34. Sun Y, Xu H, Tan B, Yi Q, Liu H, Chen T, Xiang H, Wang R, Xie Q, Tian J, Zhu J. Andrographolide protects bone marrow mesenchymal stem cells against glucose and serum deprivation under hypoxia via the NRF2 signaling pathway. Stem Cell Res Ther 2022;13: 326.
  35. Johnson JA, Johnson DA, Kraft AD, Calkins MJ, Jakel RJ, Vargas MR, Chen PC. The Nrf2–ARE pathway: an indicator and modulator of oxidative stress in neurodegeneration. Ann N Y Acad Sci 2008;1147:61-69.
  36. Afonyushkin T, Oskolkova OV, Philippova M, Resink TJ, Erne P, Binder BR, Bochkov VN. Oxidized phospholipids regulate expression of ATF4 and VEGF in endothelial cells via NRF2-dependent mechanism: novel point of convergence between electrophilic and unfolded protein stress pathways. Arterioscler Thromb Vasc Biol 2010;30:1007-1013.
  37. Pandamooz S, Salehi MS, Nabiuni M, Dargahi L, Pourghasem M. Evaluation of epidermal neural crest stem cells in organotypic spinal cord slice culture platform. Folia Biol (Praha) 2016;62:263-267.
  38. Lin H, Liu F, Zhang C, Zhang Z, Guo J, Ren C, Kong Z. Pluripotent hair follicle neural crest stem-cell-derived neurons and schwann cells functionally repair sciatic nerves in rats. Mol Neurobiol 2009;40:216-223.
  39. Pandamooz S, Jurek B, Salehi MS, Mostaghel M, Miyan JA, Dianatpour M, Borhani-Haghighi A. The implementation of preconditioned epidermal neural crest stem cells to combat ischemic stroke. Comment on Othman, FA; Tan, SC Preconditioning strategies to enhance neural stem cell-based therapy for ischemic stroke. Brain Sci. 2020, 10, 893. Brain Sci 2021;11:653.
  40. Zhang T, Yang X, Liu T, Shao J, Fu N, Yan A, Geng K, Xie W. Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice. Stem Cell Res Ther 2017;8:248.
  41. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9-19.
  42. Awadalla A, Hussein AM, El-Far YM, El-Senduny FF, Barakat N Hamam ET, Abdeen HM, El-Sherbiny M, Serria MS, Sarhan AA, Sena AM, Shokeir AA. Rapamycin Improves Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Renoprotective Effect against Cisplatin-Induced Acute Nephrotoxicity in Rats by Inhibiting the mTOR/AKT Signaling Pathway. Biomedicines 2022;10:1295.
  43. Jung KH, Han DMR, Jeong SG, Choi MR, Cahi YG, Cho GW. Proteomic analysis reveals KRIT1 as a modulator for the antioxidant effects of valproic acid in human bone-marrow mesenchymal stromal cells. Drug Chem Toxicol 2015;38:286-292.
  44. Liu M, Li H, Zhang L, Xu Z, Song Y, Wang X, Chu R, Xiao Y, Sun M, Ma Y, Mi W. Cottonseed oil alleviates ischemic stroke-induced oxidative stress injury via activating the Nrf2 signaling pathway. Mol Neurobiol 2021;58:2494-2507.
  45. Zhang W, Wei R, Zhang L, Tan Y, Qian C. Sirtuin 6 protects the brain from cerebral ischemia/reperfusion injury through NRF2 activation. Neuroscience 2017;366:95-104.
  46. Hu YF, Gourab K, Wells C, Clewes O, Schmit BD, Sieber-Blum M. Epidermal neural crest stem cell (EPI-NCSC)--mediated recovery of sensory function in a mouse model of spinal cord injury. Stem Cell Rev Rep 2010;6(2):186-98.
  47. Zuccato C, Cattaneo E. Brain-derived neurotrophic factor in neurodegenerative diseases. Nat Rev Neurol 2009;5:311-322.
  48. Saito A, Narasimhan P, Hayashi T, Okuno S, Ferrand-Drake M, Chan PH. Neuroprotective role of a proline-rich Akt substrate in apoptotic neuronal cell death after stroke: relationships with nerve growth factor. J Neurosci 2004;24:1584-1593.
  49. Neirinckx V, Coste C, Rogister B, Wislet-Gendebien S. Concise review: adult mesenchymal stem cells, adult neural crest stem cells, and therapy of neurological pathologies: a state of play. Stem Cells Transl Med 2013;2:284-296.
  50. Wu CC, Lien CC, Hou WH, Chiang PM, Tsai KJ. Gain of BDNF function in engrafted neural stem cells promotes the therapeutic potential for Alzheimer’s disease. Sci Rep 2016; 6:27358.
  51. Deng P, Anderson JD, Yu AS, Annett G, Fink KD, Nolta JA. Engineered BDNF producing cells as a potential treatment for neurologic disease. Expert Opin Biol Ther 2016;16:1025-1033.
  52. Pandamooz S, Salehi MS, Safari A, Azarpira N, Heravi M, Ahmadiani A, Dargahi L. Enhancing the expression of neurotrophic factors in epidermal neural crest stem cells by valproic acid: A potential candidate for combinatorial treatment. Neurosci Lett 2019;704:8-14.
  53. Rosenberg  MB, Friedmann T, Robertson RC, Tuszynski M, Wolff JA, Breakefield XO, Gage FH. Grafting genetically modified cells to the damaged brain: restorative effects of NGF expression. Science 1988;242:1575-1578.
آمار
تعداد مشاهده مقاله: 1,507
تعداد دریافت فایل اصل مقاله: 1,192


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب