First report of the incidence of potato virus Y in some ornamental plants in Iran | ||
| Iran Agricultural Research | ||
| مقاله 5، دوره 42، شماره 1، شهریور 2023، صفحه 47-54 اصل مقاله (347.04 K) | ||
| نوع مقاله: Note | ||
| شناسه دیجیتال (DOI): 10.22099/iar.2023.48019.1550 | ||
| نویسندگان | ||
| Mehrdad Salehzadeh1؛ Alireza Afsharifar* 1؛ Saeedeh dehghanpour farashah2 | ||
| 1Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, I. R. Iran | ||
| 2Agriculture Department, Payame Noor University, Tehran, I. R. Iran | ||
| چکیده | ||
| During the surveys conducted from green space in Shiraz City, Iran, in the summer of 2022, leaf chlorosis and mosaic symptoms were observed on the leaves of black-eyed-susans (Rudbeckia hirta), a Dahlia sp., and Mexican Petunia (Ruellia brittoniana) plants. Total genomic RNA was separately extracted from 10 symptomatic and one symptomless (negative control) leaf samples and subjected to Reverse Transcription-Polymerase Chain Reaction (RT-PCR) using a potyvirus degenerate primer pair (NIb3R, NIb2F). RT-PCR resulted in the amplification of an approximately 350 bp DNA fragment in all symptomatic samples, while no such fragment was amplified from the symptomless plant. The amplified DNA fragment was subjected to Sanger sequencing, and its size was determined to be exactly 350 bp, and confirmed that it belongs to the Nib gene of potato virus Y (PVY). The nucleotide (nt) sequence of the amplicons was compared with the nt sequence of the same region of some other PVY isolates that were available in the GenBank. The sequence analysis revealed that the R. brittoniana isolate exhibited the highest (98.8%) similarity to a PVY isolate from the USA with the Acc. No. of KY_848029.1. Similarly, the R. hirta and the Dahlia isolates showed the highest (98.3%, 98.4 %, respectively ) similarity to a PVY isolate from Kazakhstan with the Acc. No. of ON_583980.1. | ||
| کلیدواژهها | ||
| Degenerate primers؛ Green space؛ Potyvirus؛ Ornamental plants؛ Shiraz | ||
| مراجع | ||
|
Ágoston, J., Almási, A., Nemes, K., Salánki, K., & Palkovics, L. (2020). First report of hippeastrum mosaic virus, narcissus late season yellows virus, narcissus latent virus and narcissus mosaic virus in daffodils from Hungary. Journal of Plant Pathology, 102, 1275-1276. https://doi.org/10.1007/s42161-020-00556-9 Alexandre, M. A. V., Duarte, L. M., Chaves, A. L (2023). Tropical ornamental plants: Brazilian overview of viral infections and management. Ornamental Horticulture, 29(2), 249-261 https://doi.org/10.1590/2447-536X.v29i2.2613 Alexandre, M. A. V., Duarte, L. M., Chaves, A. L., & Kitajima, E. W. (2021). Potyvirus infecting ornamental plants grown in the Neotropical region. In: Raj, S. K., Gaur, R. K., Yin, Z. (Eds.). Virus Diseases of Ornamental Plants. (pp 23-59). Singapore: Springer,.https://doi.org/ 10.1007/978-981-16-3919-7_2 Alexandre, M. A. V., Duarte, L. M., Rodrigues, L. K., Ramos, A. F., & Harakava, R. (2017). Hyacinth mosaic virus infecting Hyacinthus sp. plants in Brazil. Tropical Plant Pathology, 42, 51-54. https://doi.org/10.1007/s40858-016-0121-5 Asjes, C. J. (1979). Virus and virus disease in Dutch bulbous Iris (Iris hollandica) in Netherlands, Netherlands Journal of Plant Pathology, 85, 269-279. https://doi.org/10.1007/BF01977599 Bello, V. H., Favara, G. M., Bernardi, G. V., Rezende, J. A. M., Salaroli, R. B., & Kitajima, E. W. (2022). First report of sunflower chlorotic mottle virus infecting sunflower plants in Brazil. Scientia Agricola, 80 (e20220035). ttps://doi.org/10.1590/1678-992x-2022-0035 Chen, C. C., & Chang, C. A. (1998). Characterization of a potyvirus causing mild mosaic on tuberose. Plant Disease, 82(1), 45-49. https://doi.org/10.1094/PDIS.1998.82.1.45. Chen, J., Chen, J., Chen, J. Adams M. J. (2001)> Molecular characterisation of an isolate of Dasheen mosaic virus from Zantedeschia aethiopica in China and comparisons in the genus Potyvirus . Archives of Virology. 146, 1821–1829. https://doi.org/10.1007/s007050170068 Chen, J., Lu, Y.W., Shi,Y. H., Adams, M.J. & Chen,J. P. 2006. Complete nucleotide sequence of the genomic RNA of Narcissus yellow stripe virus from Chinese narcissus in Zhangzhou city, China. Archives of Virology, 151 (8), 1673-1677. doi: 10.1007/s00705-006-0788-x Cho, S. Y., Kim, H., Yi, S. I., Lim, S., Park, J. M., Cho, H. S., ... & Moon, J. S. (2017). First report of Impatiens flower break virus infecting Impatiens walleriana in South Korea. Plant Disease, 101(2), 394. doi:10.1094/PDIS-08-16-1192-PDN Choi, S. K., Cho, I. S., Choi, G. S., & Yoon, J. Y. (2014). First report of Tomato spotted wilt virus in Brugmansia suaveolens in Korea. Plant Disease, 98(9), 1283-1283. https://doi.org/10.1094/PDIS-02-14-0173-PDN Deng, D., McGrath, P. F., Robinson, D. J., & Harrison, B. D. (1994). Detection and differentiation of whitefly‐transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of applied Biology, 125(2), 327-336. doi:10.1111/j.1744-7348.1994.tb04973.x Duarte, L. M. L., Alexandre, M. A. V., Rivas, E. B., Galleti, S. R., Harakava, R., & Chaves, A. L. R. (2014). A new Potyvirus species in Cotyledon orbiculata in mixed infection with a Nucleorhabdovirus. Journal of Plant Pathology, 96(1), 143-149. doi: 10.4454/JPP.V96I1.039 Favara, G. M., de Oliveira, F. F., Ferro, C. G., Kraide, H. D., Carmo, E. Y. N., Chinelato, G. A., ... & Marques Rezende, J. A. (2021). First report of Costus stripe mosaic virus infecting Tradescantia spathacea plants in Brazil. Plant Disease, 105(10), 3314. https://doi.org/10.1094/PDIS-02-21-0342-PDN. Gibbs, A., & Mackenzie, A. (1997). A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods, 63(1-2), 9-16. doi:10.1016/S0166-0934(96)02103-9 Green K. J., Brown C. J., Gra ,S. M. and Karasev A. V. (2017). Phylogenetic study of recombinant strains of Potato virus Y. Virology 507, 40-52. https://doi.org/10.1016/j.virol.2017.03.018 Hussain, A., Arif, M., Abbas, A., Hussain, B., Ali, M., & Jaffar, S. (2016). A review on aphid-borne virus (Potato Virus Y). Journal of Entomology and Zoology Studies, 4(3), 189-192. doi:10.13140/RG.2.1.1661.7844 Jordan, R., Guaragna, M. A., & Putnam, M. (2011). Detection and molecular characterization of new and emerging potyviruses of ornamental plants. ISHS Acta Horticulturae (XII International Symposium on Virus Diseases of Ornamental Plants), 901, 159-166. doi:10.17660/ActaHortic.2011.901.20 Koenig, R. (1984). Recently discovered virus or viruslike diseases of ornamentals and their epidemiological significance. ISHS Acta Horticulturae:(VI International Symposium on Virus Diseases of Ornamental Plants), 164, 21-32. doi:10.17660/ActaHortic.1985.164.1 Koh, S. H., Li, H., Admiraal, R., Jones, M. G., & Wylie, S. J. (2015). Catharanthus mosaic virus: A potyvirus from a gymnosperm, Welwitschia mirabilis. Virus Research, 203, 41-46. https://doi.org/10.1016/j.virusres.2015.03.007 Kraus, J., Cleveland, S., Putnam, M. L., Keller, K. E., Martin, R. R., & Tzanetakis, I. E. (2010). A new Potyvirus sp. infects verbena exhibiting leaf mottling symptoms. Plant Disease, 94(9), 1132-1136. https://doi.org/10.1094/PDIS-94-9-1132 Kulshrestha, S., Hallan, V., Raikhy, G., Ram, R., Garg, I. D., Haq, Q. M. R., & Zaidi, A. A. (2006). Occurrence of iris mild mosaic potyvirus in cultivated iris in India. Indian Journal of Biotechnology. 5, 94-98. Kumari, A., Kumar, S., Chauhan, P. S., & Raj, S. K. (2022). Elimination of coexisting canna yellow mottle virus, bean yellow mosaic virus and cucumber mosaic virus from Canna generalis cv. black knight through in vitro chemotherapy of rhizome explants. 3 Biotech, 12(10), 267. https://doi.org/10.1007/s13205-022-03330-z Li, Y., Tan, G., Lan, P., Zhang, A., Liu, Y., Li, R., & Li, F. (2018). Detection of tobamoviruses by RT-PCR using a novel pair of degenerate primers. Journal of Virological Methods, 259, 122-128. doi:10.1016/j.jviromet.2018.06.012 Liu, X. L.; Wei, Q.; Hong, B.; Zhao, X. T. (2014). First report of Potato virus Y strain N-Wilga infecting Chrysanthemum in China. Plant Disease, 98, 1589-1589. DOI: http://doi.org/10.1094/PDIS-02-14-0154-PDN Maciel, S. C., Silva, R. F. D., Reis, M. S., Jadão, A. S., Rosa, D. D., Giampan, J. S., ... & Camargo, L. E. (2015). Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil. Scientia Agricola, 68, 687-690. https://doi.org/10.1590/S0103-90162011000600013 Marys, E., & Romano, M. (2011). Occurrence of Euphorbia ringspot virus in Euphorbia milii cv. splendens in Venezuela. Journal of Phytopathology, 159(1), 66-68. doi:10.1111/j.1439-0434.2010.01709.x Mitrofanova, I. V., Zakubanskiy, A., & Mitrofanova, O. (2018). Viruses infecting main ornamental plants an overview. Ornamental Horticulture 24(2), 95-102. Mollov, D., Grinstead, S., Tahir, M. N., Reinsel, M., & Hammond, J. (2017). First report of multiple isolates of gloriosa stripe mosaic virus in Gloriosa superba in the United States. Plant Disease, 101(6), 1070-1070. https://doi.org/10.1094/PDIS-12-16-1795-PDN Pandit, M. K., Nath, P. S., Mukhopadhyay, S., Devonshire, B. J., & Jones, P. (2001). First report of Dasheen mosaic virus in elephant foot yam in India. Plant Pathology, 50(6). DOI: 10.1046/j.1365-3059.2001.00625.x Pearson, M. N., Cohen, D., Cowell, S. J., Jones, D., Blouin, A., Lebas, B. S. M., ... & Clover, G. R. G. (2009). A survey of viruses of flower bulbs in New Zealand. Australasian Plant Pathology, 38, 305-309. doi:10.1071/AP09006 Pham, K. T. K., De Kock, M. J. D., Lemmers, M. E. C., & Derks, A. F. L. M. (2011). Molecular identification of potyviruses infecting bulbous ornamentals by the analysis of coat protein (CP) sequences. ISHS Acta Horticulturae (XII International Symposium on Virus Diseases of Ornamental Plants) 901, 167-172. https://doi.org/10.17660/ActaHortic.2011.901.21 Quenouille, J., Vassilakos, N. & Moury B. (2013). Potato virus Y: a major crop pathogen that has provided major insights into the evolution of viral pathogenicity. Molecular Plant Pathology, 14(5):439-452. doi: 10.1111/mpp.12024 Raj, R., Agrawal, L., Kumar, S., Chauhan, P. S, & Raj, S. K. (2019). Complete genome sequence analysis of Narcissus yellow stripe virus infecting Narcissus tazetta in India. 3 Biotec, 9(11), 409. doi: 10.1007/s13205-019-1939-6 Raj, S. K., Snehi, S. K., Kumar, S., & Khan, M. S. (2009). First molecular detection and identification of a potyvirus associated with severe mosaic disease of amaryllis (Hippeastrum hybridum Hort.) in India. Australasian Plant Disease Notes, 4(1), 50-53. https://doi.org/10.1071/DN09021 Rizos, H., Gunn, L. V., Pares, R. D., & Gillings, M. R. (1992). Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. Journal of General Virology, 73(8), 2099-2103. doi:10.1099/0022-1317-73-8-2099 Rojas, M. R., Gilbertson, R. L., Russell, D. R., & Maxwell, D. P. (1993). Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease, 77(4), 340-347. doi:10.1094/pd-77-0340 Rott, M., Schmidt, A. M., Joshi, V., Masters, C., Godkin, S., & Johnson, R. (2009). First Report of Colombian datura virus in Brugmansia in Canada. Plant Disease, 93(2), 196. doi:10.1094/PDIS-93-2-0196B Salamon, P., & Palkovics, L. (2005). Occurrence of Colombian datura virus in Brugmansia hybrids, Physalis peruviana L. and Solanum muricatum Ait. in Hungary. Acta Virologica, 49(2), 117-122 Salehzadeh, M., Afsharifar, A., & Dehghanpour Farashah, S. (2023). First report of canna yellow streak virus in Iran. Iran Agricultural Research, 41, 159-163. doi: 10.22099/IAR.2023.47324 Sharma, P., Sahu, A. K., Verma, R. K., Mishra, R., Choudhary, D. K., & Gaur, R. K. (2013). Current status of Potyvirus in India. Archives of Phytopathology and Plant Protection, 47(8), 906-918. https://doi.org/10.1080/03235408.2013.825963 Steele, V., Thomas, J. E. (2009). First report of Colombian datura virus from Australia. Australasian Plant Disease Notes, 4, 108–109. https://doi.org/10.1071/DN09045 Valverde, R. A., Sabanadzovic, S., & Hammond, J. (2012). Viruses that enhance the aesthetics of some ornamental plants: beauty or beast? Plant Disease, 96(5), 600-611. doi:10.1094/PDIS-11-11-0928-FE Verma, R. K., Mishra, R., & Gaur, R. K. (2014). First Report of Colombian datura virus in India. New Disease Reports, 30(29), 2044-0588. https://doi.org/10.5197/j.2044-0588.2014.030.029 Ward, L. I., Veerakone, S., Tang, J., & Clover, G. R. G. (2009). First report of Narcissus degeneration virus, Narcissus late season yellows virus, and Narcissus symptomless virus on Narcissus in New Zealand. Plant Disease, 93(9), 964-964. https://doi.org/10.1094/PDIS-93-9-0964A
| ||
|
آمار تعداد مشاهده مقاله: 367 تعداد دریافت فایل اصل مقاله: 556 |
||