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Establishment of primary cystic echinococcosis in laboratory mice: our results in the Balb/c strain | ||
| Iranian Journal of Veterinary Research | ||
| دوره 23، شماره 3 - شماره پیاپی 80، 2022، صفحه 265-269 اصل مقاله (301.69 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2022.6820 | ||
| نویسندگان | ||
| Sh. A. Sohraby1؛ M. Moazeni* 2؛ E. Rakhshandehroo2 | ||
| 1Ph.D. Student in Parasitology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 2Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| Background: Establishment of experimental cystic echinococcosis (CE) in laboratory mice is required for the study of CE. Experimental CE may be established in primary or secondary forms; however, the primary form is more reliable for the study of CE. Aims: The aim of the study was to assess the possibility of establishment of primary CE in Balb/c mice via the oral administration of Echinococcus granulosus s.l. eggs. Methods: E. granulosus s.s. eggs were obtained from the gravid segments of the adult worms collected from an experimentally infected dog. Thirty-four female Balb/c mice were allocated into 4 groups and were orally infected with E. granulosus s.s. eggs as follows: 1) the mice with normal immunity, a single dose of 1000 eggs, 2) the mice with normal immunity, 3 doses of 500 eggs, 3) immunosuppressed mice, a single dose of 1000 eggs, and 4) immunosuppressed mice, 3 doses of 500 eggs. After 6.5 months, all the mice were opened and their internal organs were examined for the presence of CE cysts. The livers of infected mice were also examined for the presence of E. granulosus s.l. compartments by the PCR method. Results: There was no developed or developing CE cyst in the abdominal cavities or on the internal organs of all the mice in four groups. In addition, in the molecular study, all the examined liver samples were negative for the parasite material. Conclusion: The results of the present study revealed that Balb/c mice are not a suitable host for establishment of primary CE following the oral administration of E. granulosus s.s. eggs. | ||
| کلیدواژهها | ||
| Balb/c؛ Echinococcus granulosus s.l؛ Laboratory mice؛ Primary cystic echinococcosis | ||
| مراجع | ||
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Ahmadnia, S; Moazeni, M and Mohammadi-Samani, S (2014). Hydatid cyst formation in male Balb/c mice following the intraperitoneal injection of live protoscoleces and activated oncospheres: a comparative study. J. Parasit. Dis., 38: 77-80.
Barnett, LE (1936). White mice and experimental echino-coccosis. A.N.Z. J. Surg., 5: 323.
Breijo, M; Anesetti, G; Martinez, L; Sim, RB and Ferreira, AM (2008). Echinococcus granulosus: the establishment of the metacestode is associated with control of complement-mediated early inflammation. Exp. Parasitol., 118: 188-196.
Brunetti, E; Praticò, L; Neumayr, A; Maestri, M and Tamarozzi, F (2016). Update on treatment for cystic echinococcosis of the liver. Curr. Treat. Options Infect. Dis., 8: 153-164.
Colli, CW and Schantz, PM (1974). Growth and development of Echinococcus granulosus from embryophores in an abnormal host (Mus musculus). J. Parasitol., 58: 422-426.
Colli, CW and Williams, JF (1972). Influence of temperature on infectivity of eggs of Echinococcus granulosus in laboratory rodents. J. Parasitol., 58: 422-426.
Conchedda, M; Gabriele, F and Bortoletti, G (2004). Immunobiology of cystic echinococcosis. Parassitologia. 46: 375-380.
Dempster, RP; Berridge, MV; Harrison, GB and Heath, DD (1991). Echinococcus granulosus: development of an intermediate host mouse model for use in vaccination studies. Int. J. Parasitol., 21: 549-554.
Gómez, R; Allaoua, Y; Colmenares, R; Gil, S; Roquero, P and Ramia, JM (2016). Hydatid cyst of the gallbaldder: A systematic review of the literature. World J. Hepatol., 8: 1087-1092.
Heath, DD (1991). Daily egg-production of dogs infected with Echinococcus granulosus. Arch. Hidatidosis. 30: 321-328.
McManus, DP; Gray, DJ; Zhang, W and Yang, Y (2012). Diagnosis, treatment, and management of echinococcosis. B.M.J., 344: e3866.
Mourglia-Ettlin, G; Cucher, M; Arbildi, P; Rosenzvit, M and Demattei, S (2016a). Natural and induced antibodies contribute to differential susceptibility to secondary cystic echinococcosis of Balb/c and C57Bl/6 mice. Immunobiology. 221: 103-115.
Mourglia-Ettlin, G; Merlino, A; Capurro, R and Dematteis, S (2016b). Susceptibility and resistance to Echinococcus granulosus infection: Associations between mouse strains and early peritoneal immune responses. Immunobiology. 221: 418-426.
Rahimi, HR; Sarkari, B; Mohammadzadeh, T and Sadjjadi, SM (2011). Immune responses to antigens of in vitro reared Echinococcus granulosus adult worms in Balb/c mice. Iran J. Immunol., 8: 236-243.
Romig, T and Bilger, B (1999). Animal models for Echinococcosis. In Handbook of animal models of infection. PP: 877-884.
Salih, AM; Kakamad, FH; Hammood, ZD; Yasin, B and Ahmed, DM (2017). Abdominal wall Hydatid cyst: A review a literature with a case report. Int. J. Surg. Case Rep., 37: 154-156.
Schwabe, CW (1968). Larval Echinococcus infection in laboratory animals. Bulletin of the World Health Organization. 39: 126-127.
Smyth, JD and Howkins, AB (1966). An in vitro technique for the production of eggs of Echinococcus granulosus by maturation of partly developed strobila. Parasitology. 56: 763-766.
Yamashita, JIRO (1968). Development of Echinococcus in laboratory animals. Bulletin of the World Health Organization. 39: 127-130.
Yamashita, J; Ohbayashi, M; Kitamura, Y; Suzuki, K and Okugi, M (1958). Studies on echinococcosis VIII. Experimental Echinococcosis multilocularis in various rodents; especially on the difference of susceptibility among uniform strains of the mouse. JPN. J. Vet. Res., 6: 135-155.
Yamashita, J; Ohbayashi, M and Konno, S (1956). Studies on Echinococcosis IV. Experimental infection of the white mouse. JPN. J. Vet. Res., 4: 123-128.
Yamashita, J; Ohbayashi, M and Konno, S (1957). Studies on Echinococcosis VI: Secondary Echinococcosis multilocularis in Mice. JPN. J. Vet. Res., 5: 197-202.
Zak, O and Sande, MA (1999). Hand book of animal models of infection. In: Romig, T and Bilger, B (Eds.), Animal models for echinococcosis. (1st Edn.), San Diego, London, Boston, Academic Press. PP: 877-884.
Zhang, W; Ross, AG and McManus, DP (2008). Mechanisms of immunity in hydatid disease: implications for vaccine development. J. Immunol., 181: 6679-6685.
Zhang, W; You, H; Zhang, Z; Turson, G; Hasyet, A and McManus, DP (2001). Further studies on an intermediate host murine model showing that a primary Echinococcus granulosus infection is protective against subsequent oncospheral challenge. Parasitol. Int., 50: 279-283. | ||
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