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Soman, Smitha, Ram, Siya. (1401). MiaA (Rv2727c) mediated tRNA isopentenylation of Mycobacterium tuberculosis H37Rv. , 11(2), 97-104. doi: 10.22099/mbrc.2022.43197.1726
Smitha Soman; Siya Ram. "MiaA (Rv2727c) mediated tRNA isopentenylation of Mycobacterium tuberculosis H37Rv". , 11, 2, 1401, 97-104. doi: 10.22099/mbrc.2022.43197.1726
Soman, Smitha, Ram, Siya. (1401). 'MiaA (Rv2727c) mediated tRNA isopentenylation of Mycobacterium tuberculosis H37Rv', , 11(2), pp. 97-104. doi: 10.22099/mbrc.2022.43197.1726
Soman, Smitha, Ram, Siya. MiaA (Rv2727c) mediated tRNA isopentenylation of Mycobacterium tuberculosis H37Rv. , 1401; 11(2): 97-104. doi: 10.22099/mbrc.2022.43197.1726

MiaA (Rv2727c) mediated tRNA isopentenylation of Mycobacterium tuberculosis H37Rv

Molecular Biology Research Communications
دوره 11، شماره 2، 2022، صفحه 97-104    اصل مقاله (342.55 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2022.43197.1726
نویسندگان
Smitha Soman1؛ Siya Ram* 1، 2
1School of Biotechnology, Gautam Buddha University, Gautam Budh Nagar, Greater Noida, Uttar Pradesh, India
2School of Sciences, Indira Gandhi National Open University, Maidan Garhi, New Delhi, India
چکیده
tRNA modifications play a significant role in the structural stability as well as translational fidelity in all organisms from bacteria to humans. They also play a major role in bacterial physiology by regulating translation in response to various environmental stresses. Modifications coming at the anticodon-stem loop (ASL) are particularly important as they stabilize codon-anticodon interactions, ensuring accuracy and speed in decoding mRNAs  Addition of isopentenyl group (i6A) at A37 position by tRNA isopentenyltransferase (MiaA) is a well conserved modification from bacteria to human. We studied M. tuberculosis MiaA from strain H37Rv and identified the target tRNAs for this modification based on the A36A37A38 motif. i6A modification of target tRNAs tRNALeuCAA, tRNAPheGAA, tRNATrpCCA and tRNASerCGA were further confirmed by isopentenyltransferase assay providing the substrate DMAPP and recombinant MiaA enzyme.
کلیدواژه‌ها
Tuberculosis؛ M. smegmatis؛ MiaA, i6A؛ DMAPP؛ Isopentenyl transferase
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