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Taiwo, Odunayo Anthonia, Dosumu, Oluwatosin Adebisi, Ugbaja, Regina Ngozi, Rotimi, Solomon Oladapo, Owolabi, Oluwafemi Paul, Ojo, Oluwafemi Adeleke. (1399). Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats. , 10(1), 5-11. doi: 10.22099/mbrc.2020.38601.1557
Odunayo Anthonia Taiwo; Oluwatosin Adebisi Dosumu; Regina Ngozi Ugbaja; Solomon Oladapo Rotimi; Oluwafemi Paul Owolabi; Oluwafemi Adeleke Ojo. "Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats". , 10, 1, 1399, 5-11. doi: 10.22099/mbrc.2020.38601.1557
Taiwo, Odunayo Anthonia, Dosumu, Oluwatosin Adebisi, Ugbaja, Regina Ngozi, Rotimi, Solomon Oladapo, Owolabi, Oluwafemi Paul, Ojo, Oluwafemi Adeleke. (1399). 'Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats', , 10(1), pp. 5-11. doi: 10.22099/mbrc.2020.38601.1557
Taiwo, Odunayo Anthonia, Dosumu, Oluwatosin Adebisi, Ugbaja, Regina Ngozi, Rotimi, Solomon Oladapo, Owolabi, Oluwafemi Paul, Ojo, Oluwafemi Adeleke. Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats. , 1399; 10(1): 5-11. doi: 10.22099/mbrc.2020.38601.1557

Oral administration of marijuana produces alterations in serotonin 5-hydroxytryptamine receptor 3A gene (HTR3A) and electrolyte imbalances in brain of male Wistar rats

Molecular Biology Research Communications
دوره 10، شماره 1، 2021، صفحه 5-11    اصل مقاله (386.05 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2020.38601.1557
نویسندگان
Odunayo Anthonia Taiwo* 1، 2؛ Oluwatosin Adebisi Dosumu2؛ Regina Ngozi Ugbaja2؛ Solomon Oladapo Rotimi3؛ Oluwafemi Paul Owolabi2؛ Oluwafemi Adeleke Ojo* 4
1Department of Biochemistry, Chrisland University, Owode, Abeokuta, Nigeria
2Department of Biochemistry, Federal University of Agriculture, Abeokuta, Nigeria
3Department of Biochemistry, Covenant University, Ota, Nigeria
4Department of Biochemistry, Landmark University, Omu-Aran, Nigeria
چکیده
The gene expression of serotonin 5-hydroxytryptamine receptor 3A (receptor 3A:HTR3A) as well as the concentration of electrolytes in male Wistar rats after administration of graded doses of marijuana extract was investigated. Twelve groups (3 control and 9 test groups) of 6 animals each were daily exposed to 12.5, 25 and 50 mg/kg b.w doses of petroleum ether extract of marijuana for 4, 8 and 12 weeks. The expressions of the gene were obtained using reverse transcriptase-polymerase chain reaction (RT-PCR) while electrolytes concentrations were determined. An upregulation of over 90% was observed in the expression of HTR3A after exposure to the highest dose throughout the exposure period. There was significant increase in the plasma potassium concentration at all doses while there was a decrease in the brain only at 50 mg/kg dose throughout the exposure period. Sodium concentration in the brain was not affected by the doses over the period of exposure but plasma concentration decreased significantly. All the doses of marijuana extract significantly increased calcium concentration in the brain after prolonged exposure but the plasma concentration remained unchanged. This suggests that different doses of marijuana extract alter the expression of serotonin receptor and electrolyte concentrations over a period of time with possible neurological consequences.
کلیدواژه‌ها
Marijuana؛ Serotonin receptor gene؛ Gene expression؛ Neurotransmission
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مراجع

1. Bloom FE, Kupfer DJ. Psychopharmacology: the fourth generation of progress. New York: Raven Press 1995;407-471.

2. Reeves DC, Lummis SC. The molecular basis of the structure and function of the 5-HT3 receptor: a model ligand-gated ion channel Mol Membr Biol 2002;19:11-26.

 3. Bruss M, Eucker T, Gothert M, Bonisch H. Exon-intron organization of the human 5-HT-3A receptor gene. Neuropharmacology 2000;39:308-315.

 4. Thompson AJ, Lummis SC. 5-HT3 Receptors. Curr Pharm Des 2006;12:3615-3630.

 5. Yakel JL, Endo M, Kurachi Y, Mishina M. The 5-HT3 receptor channel: function, activation and regulation in Pharmacology of Ionic Channel Function: Activators and Inhibitors (Handbook of Experimental Pharmacology). Berlin: Springer-Verlag. ISBN 3-540-66127-1, 2000;541-560.

 6. Férézou I, Cauli B, Hill EL, Rossier J, Hamel E, Lambolez B. 5-HT3 receptors mediate serotonergic fast synaptic excitation of neocortical vasoactive intestinal peptide/cholecystokinin interneurons. J Neurosci 2002;22:7389-7397.

7. Thompson AJ, Lummis SC. The 5-HT3 receptor as a therapeutic target. Expert Opin  Ther Targets 2007;11:527-540.

8. Syzdek J, Borkowska R, Perzyna K, Tarascon JM, Wieczorek W. Novel composite polymeric electrolytes with surface-modified inorganic fillers. J Power Sources 2007;173:712-720.

9. Silverthorn DU. Human Physiology: An Integrated Approach (7th ed.). Pearson Education. ISBN 978-0-321-98122-6,2014;646-647.

10. Meier MH, Caspi A, Ambler A, Harrington H, Houts R, Keefe RSE, McDonald K, Ward A, Poulton R, Moffitt TE. Persistent cannabis users show neuropsychological decline from childhood to midlife. Proc Natl Acad Sci USA 2012;109:E2657-2664.

11. Dosumu OA, Taiwo OA, Akinloye OA, Oni EO, Owolabi OP, Ojo OA. Time-course effects of Cannabis sativa on brain acetylcholinesterase (AChE) activity and expression of dopa decarboxylase gene (DDC). PhOL 2020;2:302-313.

12. Hejazi SH, Ahangari G, Deezagi A. Alternative view point against breast cancer based on selective serotonin receptors 5HTR3A and 5HTR2A antagonists that can mediate apoptosis in MCF-7 cell line. Curr Drug Discov Technol 2015;12:240-249.

13. Kaiser R, Tremblay P, Sezer O, Possinger K, Root I, Brockmöller J. Investigation of the association between 5-HT3A receptor gene polymorphisms and efficiency of antiemetic treatment with 5-HT3 receptor antagonists. Pharmacogenetics 2004;14:271-278.

14. Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology (5th edn.), Churchill Livingstone, London, 2003; 187.

15. Aapro M, Blower P. 5-hydroxytryptamine type-3 receptor antagonists for chemotherapy-induced and radiotherapy-induced nausea and vomiting. Cancer. 2005;104:1-13.

16. Pathy M. Principles and practice of geriatric medicine (4th edition). John Wiley and sons Chichester., 2006.

17. Chatterjee K, Anderson M, Heistad D, Kerber I, Richard E. Manual of Heart Failure. JP Medical Ltd.,2014;142.

18. Osadolor HB, Emokpae AM. Effects of marijuana on sodium and potassium (Na+& K+) ions homeostasis among smokers in benin city- a metropolitan city in Nigeria. Int J Pharm Bio Sci. 2010;1:1-3

19. Hubbard JR, France SE, Onaivi E. Marijuana medical implication. Am Fam Physician 1999;1:12-15.

20. Zieg J, Gonsorcikova L, Landau D. Current views on the diagnosis and management of hypokalaemia in children. Acta Paediatr 2016;105:762-767.

21. Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology (5th edn.), Churchill Livingstone, London., 2003;608-610.

22. Ghovanloo MR, Shuart NG, Mezeyova J, Dean R, Ruben PC, Goodchild SJ. Inhibitory effects of cannabidiol on voltage-dependent sodium currents. J Biol Chem 2018;293:16546-16558.

23. Henry DA. In The Clinic: Hyponatremia. Ann Intern Med 2015;163:1-19.

24. Ball S, De Groot LJ, Beck-Peccoz P, Chrousos G, Dungan K, Grossman A, Singhi S, Dhawan A.. Hyponatremia.  J Biol Chem 2000;267:5735-5738.

25. Abam E, Okediran BS, Odukoya OO, Adamson I, Ademuyiwa O. Reversal of ionoregulatory disruptions in occupational lead exposure by vitamin C. Environ Toxicol Pharmacol 2008;26:297-304.

26. Timbrell J. Principles of Biochemical Toxicology, 3rd ed. Taylor and Francis  2000;9-25.

27. Gregus Z, Klaassen CD. Mechanisms of toxicity. In: Klaassen, CD, Watkins III, J.B. (Eds.), Casarett and Doull’s Essentials of Toxicology. McGraw-Hill, New York,2003;21-45.

28. Hillard CJ, Muthian S, Kearn CS. Effects of CB1 cannabinoid receptor activation on cerebellar granule cell nitric oxide synthase activity. FEBS Letters 1999;459:277-281.

29. Jahnen-Dechent Wilhelm, Ketteler M. Magnesium basics. Clin Kidney J 2012;5:3-14.

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