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Diagnosis of mixed gastrointestinal nematode infection in goat by an indirect-ELISA | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 6، دوره 19، شماره 3 - شماره پیاپی 64، 2018، صفحه 189-193 اصل مقاله (215.87 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2018.4937 | ||
| نویسندگان | ||
| S. Datta1؛ P. Dandapat2؛ R. Jas* 3 | ||
| 1Veterinary Officer, Block Animal Health Centre, Barasat Block I, Barasat-700 128, Government of West Bengal | ||
| 2Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata-700 037, West Bengal, India | ||
| 3Department of Veterinary Parasitology, Faculty of Veterinary & Animal Sciences, West Bengal University of Animal & Fishery Sciences, 37, Kshudiram Bose Sarani, Kolkata-700 037, India | ||
| چکیده | ||
| An indirect-ELISA for the diagnosis of mixed gastrointestinal (GI) nematode infection comprising Oesophagostomum, Haemonchus and Trichuris species was standardized using crude somatic antigen of Oesophagostomum columbianum (CSAg-Oc) and sera of slaughtered goats with known parasitological status including Oesophagostomum, Haemonchus, and Trichuris (strong positive), Haemonchus and Trichuris (weak positive) and parasite free goats (negative). Two cut-off points, i.e. higher and lower cut-off were determined using the strong positive, weak positive and the negative control sera of goats. Thus the test sera having optical density (OD) values greater than the higher cut-off were considered positive for mixed infection with all the three nematode species, intermediate between the higher and the lower cut-off values were considered positive for mixed infection of Haemonchus and Trichuris, and less than the lower cut-off value were considered negative for any of these three nematode species. The sensitivity, specificity and accuracy of the ELISA for diagnosis of mixed GI nematodoses were 81.25, 93.18% and 90.00%, respectively, while it was 92.86% sensitive, 75.00% specific and 91.67% accurate for the diagnosis of mixed infection with Haemonchus and Trichuris. The ELISA, so standardized, detected 27.78% sero-prevalence of Oesophagostomum plus Haemonchus and Trichuris infection and 38.89% percent of Haemonchus and Trichuris infection in the field goats. The standardized assay might be exploited as a diagnostic tool and also for sero-epidemiological study of two important GI nematodes of goats. | ||
| کلیدواژهها | ||
| Diagnosis؛ Goat؛ Haemonchus؛ Indirect-ELISA؛ Oesophagostomum | ||
| مراجع | ||
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