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Gharibi, S., Niasari-Naslaji, A., Poursasan, N., Moosavi-Movahedi, A. A.. (1393). Replacement of salamon with shotor diluent and egg yolk with low density lipoprotein for chilled storage of ram semen. , 15(3), 279-284. doi: 10.22099/ijvr.2014.2540
S. Gharibi; A. Niasari-Naslaji; N. Poursasan; A. A. Moosavi-Movahedi. "Replacement of salamon with shotor diluent and egg yolk with low density lipoprotein for chilled storage of ram semen". , 15, 3, 1393, 279-284. doi: 10.22099/ijvr.2014.2540
Gharibi, S., Niasari-Naslaji, A., Poursasan, N., Moosavi-Movahedi, A. A.. (1393). 'Replacement of salamon with shotor diluent and egg yolk with low density lipoprotein for chilled storage of ram semen', , 15(3), pp. 279-284. doi: 10.22099/ijvr.2014.2540
Gharibi, S., Niasari-Naslaji, A., Poursasan, N., Moosavi-Movahedi, A. A.. Replacement of salamon with shotor diluent and egg yolk with low density lipoprotein for chilled storage of ram semen. , 1393; 15(3): 279-284. doi: 10.22099/ijvr.2014.2540

Replacement of salamon with shotor diluent and egg yolk with low density lipoprotein for chilled storage of ram semen

Iranian Journal of Veterinary Research
مقاله 15، دوره 15، شماره 3 - شماره پیاپی 48، 2014، صفحه 279-284    اصل مقاله (126.22 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2014.2540
نویسندگان
S. Gharibi1؛ A. Niasari-Naslaji* 1؛ N. Poursasan2؛ A. A. Moosavi-Movahedi3
1Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2BSc in Biology, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
3Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
چکیده
The present study investigated the possibility of replacing salamon with modified shotor diluent (MSD) and egg yolk (EY) with low density lipoprotein (LDL) for chilled storage of ram semen. Good quality semen (>80% progressive forward motility (PFM) of sperm) from 3 fertile rams was collected using an artificial vagina and pooled for each experiment. Low density lipoprotein was extracted from fresh EY. In experiment 1, semen was divided into 2 fractions and extended in MSD or salamon. In experiment 2, semen was assigned into 5 fractions and extended in MSD supplemented with 12 and 15% EY or 3, 5 and 8% LDL. In experiment 3, semen was divided into 2 fractions and extended in MSD supplemented with 12% EY or 5% LDL. Viability of sperm was assessed at times 0 (immediately after semen dilution), 2 or 4 (at 4°C) and up to 72 h after semen dilution. Data was analyzed using General Linear Model (GLM) procedure, including repeated measures. In experiment 1, the viability of sperm was similar in two diluents (P>0.05). In experiment 2, PFM of sperm was similar among groups at the time of dilution (P>0.05); but remained elevated in 5 and 8% LDL compared to other groups afterward (P<0.05). In experiment 3, PFM of sperm was superior at 48 and 72 h after dilution in 5% LDL compared to 12% EY (P<0.05). In conclusion, MSD supplemented with 5% LDL is a suitable diluent for ram fresh semen preserved at 4°C for 72 h.
کلیدواژه‌ها
Ram؛ Semen preservation؛ Low density lipoprotein؛ Diluent
آمار
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