آمار

تعداد نشریات24
تعداد شماره‌ها847
تعداد مقالات7,528
تعداد مشاهده مقاله13,264,878
تعداد دریافت فایل اصل مقاله11,507,825
Ghanbari, M., Rezaei, M., Soltani, M., Shah-Hosseini, Gh.. (1388). Production of bacteriocin by a novel Bacillus sp. strain RF 140, an intestinal bacterium of Caspian Frisian Roach (Rutilus frisii kutum). , 10(3), 267-272. doi: 10.22099/ijvr.2009.1703
M. Ghanbari; M. Rezaei; M. Soltani; Gh. Shah-Hosseini. "Production of bacteriocin by a novel Bacillus sp. strain RF 140, an intestinal bacterium of Caspian Frisian Roach (Rutilus frisii kutum)". , 10, 3, 1388, 267-272. doi: 10.22099/ijvr.2009.1703
Ghanbari, M., Rezaei, M., Soltani, M., Shah-Hosseini, Gh.. (1388). 'Production of bacteriocin by a novel Bacillus sp. strain RF 140, an intestinal bacterium of Caspian Frisian Roach (Rutilus frisii kutum)', , 10(3), pp. 267-272. doi: 10.22099/ijvr.2009.1703
Ghanbari, M., Rezaei, M., Soltani, M., Shah-Hosseini, Gh.. Production of bacteriocin by a novel Bacillus sp. strain RF 140, an intestinal bacterium of Caspian Frisian Roach (Rutilus frisii kutum). , 1388; 10(3): 267-272. doi: 10.22099/ijvr.2009.1703

Production of bacteriocin by a novel Bacillus sp. strain RF 140, an intestinal bacterium of Caspian Frisian Roach (Rutilus frisii kutum)

Iranian Journal of Veterinary Research
مقاله 10، دوره 10، شماره 3 - شماره پیاپی 28، 2009، صفحه 267-272    اصل مقاله (143.18 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2009.1703
نویسندگان
M. Ghanbari1؛ M. Rezaei* 1؛ M. Soltani2؛ Gh. Shah-Hosseini3
1Department of Fisheries, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Mazandaran, Iran
2Department of Health Hygiene and Aquatic Disease, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3Department of Nuclear Agriculture Research, School of Agricultural, Medical and Industrial, Nuclear Science Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Karaj, Iran
چکیده
Bacteriocins are proteinaceous antibacterial compounds that exhibit bactericidal activity against species closely related to the producer strain. The aim of this research was to investigate the production of bacteriocin by Bacillus spp. isolated from intestinal bacterial flora of the Caspian Frisian Roach (Rutilus frisii kutum). A bacteriocin produced by the bacterium Bacillus cereus strain RF 140 was identified. The antimicrobial activity started at the exponential growth phase and maximum activity was at the stationary growth phase. A crude bacteriocin obtained from culture supernatant fluid was inhibitory to indicator strains,including Listeria monocytogenes, Clostridium perfringens, and several species of Bacillus. Bacteriocin was stable at 80°C, but the activity decreased and was lost when the temperature reached 100 and 121°C, respectively. It was resistant to the proteolytic action of papain, catalase and amylase, but sensitive to proteinase K, pronase E and trypsin. Maximum bacteriocin activity was observed in the pH 4-9. This study indicates the importance of the bacteriocin produced by B. cereus  strain RF 140 against food-borne pathogenic microorganisms such as L. monocytogenes and C. perfringens , and presents a potential for use as a biopreservative in food.
 
 
کلیدواژه‌ها
Rutilus frisii kutum؛ Bacillus cereus؛ Bacteriocin؛ Listeria monocytogenes؛ Clostridium perfringens
آمار
تعداد مشاهده مقاله: 1,869
تعداد دریافت فایل اصل مقاله: 1,872


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب