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Effect of ±5ºC difference from physiologic temperature on surface area, volume, and surface-to-volume ratio of transformed cells | ||
Iranian Journal of Science | ||
مقاله 8، دوره 37، شماره 2، مرداد 2013، صفحه 161-164 اصل مقاله (162.2 K) | ||
نوع مقاله: Regular Paper | ||
شناسه دیجیتال (DOI): 10.22099/ijsts.2013.1505 | ||
نویسندگان | ||
M. Rezaei-Tavirani* 1؛ s. Kalantari11؛ S. Hessami Tackallou2؛ R. Rastmanesh3 | ||
1Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
2Department of Biology, Islamic Azad University, Garmsar Branch, Garmsar, Iran | ||
3Human Nutrition Department, Faculty of Nutrition, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
چکیده | ||
Information on the relationship between culture temperature and changes in the cell's area, volume and area to volume (S/V) ratio is lacking. Cancerous epithelial-like cell lines SW480, SW742 and T-47D were cultured at 32, 37 and 42°C for 48hr. Digital images of cells were analyzed using software Imaged. Observed surface area, volume changes, and surface area to volume (S/V) ratio were significantly different at 32, 37 and 42°C in the three cell-lines tested (P<0.05). S/V ratios for all three cell lines at 37°C were significantly greater than that of at 42°C (P=0.0001). | ||
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