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PLLA/HA Nano composite scaffolds for stem cell proliferation and differentiation in tissue engineering | ||
| Molecular Biology Research Communications | ||
| مقاله 1، دوره 2، 1-2، تیر 2013، صفحه 1-10 اصل مقاله (317.21 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2013.1459 | ||
| نویسندگان | ||
| Fariba Mansourizadeh1؛ Asadollah Asadi* 2؛ Shahrbanoo Oryan1؛ Ali Nematollahzadeh3؛ Masoumeh Dodel4؛ Mehdi Asghari-Vostakolaei5 | ||
| 1Department of Biology, University of Kharazmi, Tehran, Iran | ||
| 2Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 3Chemical Engineering Department, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 4Nanotechnology and Tissue Engineering Department, Stem Cell Technology Research center, Tehran, Iran | ||
| 5Bone tissue engeneering, Cell therapy research center, ACECR Ardabil branch | ||
| چکیده | ||
| Abstract Due to their mulitpotency, Mesenchymal stem cells (MSCs), have the ability to proliferate and differentiate into multiple mesodermal tissues. The aim of this study was to isolate MSCs from human Umbilical Cord (hUCMSCs) to determine their osteogenic potential on nanofibrous scaffolds. To this end, Poly (L-lactic acid) (PLLA)/Nano hydroxyapatite (HA) composite nanofibrous scaffolds were prepared by electrospinning. The structure and morphology of the scaffolds were investigated using scanning electron microscopy. Human mesenchymal stem cells (MSCs) were isolated from the umbilical cords and cultured in the PLLA/HA scaffold. The viability and proliferation of the cells was then determined by an MTT assay. Cellular adhesion, proliferation and osteogenic differentiation were assessed in these constructs using a range of histological and microscopic techniques. The osteogenesis assays indicated the superiority of nanofibrous scaffolds in supporting MSCs undergoing bone differentiation. Collectively, the bone construct prepared with PLLA/HA scaffold and proliferated MSCs would be a suitable candidate for use in bone regenerative medicine. Keywords: hUCMSCs; nanofibrous scaffolds; hydroxyapatite; PLLA; Cell proliferation; Bone differentiation. | ||
| کلیدواژهها | ||
| hUCMSCs؛ Nanofibrous scaffolds؛ Hydroxyapatite؛ PLLA؛ Cell proliferation؛ Bone differentiation | ||
| مراجع | ||
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1. Butler DL, Goldstein SA, Guilak F. Functional tissue engineering: the role of biomechanics. J Biomech Eng 2000;122:570
2. Wang X, Song G, Lou T. Fabrication and characterization of nano-composite scaffold of PLLA/silane modified hydroxyapatite. Med Eng Phys 2010;32:391-397.
3. Chen JP, Su CH. Surface modification of electrospun PLLA nanofibers by plasma treatment and cationized gelatin immobilization for cartilage tissue engineering. Acta Biomater 2011;7:234-243.
4. Sultana N, Kadir MRA. Study of in vitro degradation of biodegradable polymer based thin films and tissue engineering scaffolds. Afr J Biotechnol 2011;10:18709-18715.
5. Liao S, Chan CK, Ramakrishna S. Stem cells and biomimetic materials strategies for tissue engineering. Materials Sci Eng C Mater Biol Appl 2008;28:1189-1202.
6. Pham QP, Sharma U, Mikos AG. Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue Eng 2006;12:1197-1211.
7. Liu X, Won Y, Ma PX. Surface modification of interconnected porous scaffolds. J Biomed Mater Res Part A 2005;74:84-91.
8. Sui G, Yang X, Mei F, Hu X, Chen G, Deng X, et al. Poly‐L‐lactic acid/hydroxyapatite hybrid membrane for bone tissue regeneration. J Biomed Mater Res Part A 2007; 82:445-454.
9. Hong Z, Reis RL, Mano JF. Preparation and in vitro characterization of scaffolds of poly (L-lactic acid) containing bioactive glass ceramic nanoparticles. Acta Biomater 2008;4: 1297-1306.
10. Eslaminejad MB, Bagheri F, Zandi M, Nejati E, Zomorodian E, Mivehchi H. Comparison of proliferation and osteoblast differentiation of marrow-derived mesenchymal stem cells on nano-and micro-hydroxyapatite contained composite scaffolds. Iran J Biotechnol 2010;8:234-242.
11. Tian T, Jiang D, Zhang J, Lin Q. Fabrication of bioactive composite by developing PLLA onto the framework of sintered HA scaffold. Mater Sci Eng: C 2008;28:51-6.
12. Schneider RK, Puellen A, Kramann R, Raupach K, Bornemann J, Knuechel R, Pérez-Bouza A, Neuss S. The osteogenic differentiation of adult bone marrow and perinatal umbilical mesenchymal stem cells and matrix remodeling in three-dimensional collagen scaffolds. Biomaterials 2010;31:467-480.
13. Secco M, Zucconi E, Vieira NM, Fogaça LL, Cerqueira A, Carvalho MD, Jazedje T, Okamoto OK, Muotri AR, Zatz M. Multipotent stem cells from umbilical cord: cord is richer than blood. Stem Cells 2007;26:146-150.
14. Troyer DL, Weiss ML. Concise Review: Wharton's Jelly‐Derived Cells Are a Primitive Stromal Cell Population. Stem Cells 2007;26:591-599
15. Qiao C, Xu W, Zhu W, Hu J, Qian H, Yin Q, Jiang R, Yan Y, Mao F, Yang H, Wang X, Chen Y. Human mesenchymal stem cells isolated from the umbilical cord. Cell Biol Int 2008;32:8-15.
16. Chuenjitkuntaworn B, Supaphol P, Pavasant P, Damrongsri D. Electrospun poly (L‐lactic acid)/hydroxyapatite composite fibrous scaffolds for bone tissue engineering. Polymer Int 2010;59:227-235.
17. Chen JP, Su CH. Surface modification of electrospun PLLA nanofibers by plasma treatment and cationized gelatin immobilization for cartilage tissue engineering. Acta Biomater 2011;7:234-243.
18. Spadaccio C, Rainer A, Trombetta M, Vadalá G, Chello M, Covino E, Denaro V, Toyoda Y, Genovese JA.Poly-L-lactic acid/hydroxyapatite electrospunnanocomposites induce chondrogenic differentiation of human MSC. Ann Biomed Eng 2009;37:1376-1389.
19. Liu Y, Peterson DA, Kimura H, Schubert D. Mechanism of cellular 3‐(4,5‐dimethylthia-zol‐2‐yl)‐ 2, 5‐ diphenyltetrazolium bromide (MTT) reduction. J Neurochemistry 1997;69:581-593.
20. Lao L, Wang Y, Zhu Y, Zhang Y, Gao C. Poly (lactide-co-glycolide)/hydroxyapatite nanofibrous scaffolds fabricated by electrospinning for bone tissue engineering. J Mater Sci Mater Med 2011;22:1873-1884.
21. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143-147.
22. Wegman F, Bijenhof A, Schuijff L, Öner F, Dhert W, Alblas J. Osteogenic differentiation as a result of BMP-2 plasmid DNA based gene therapy in vitro and in vivo. Eur Cells Materials 2011;21:230-242.
23. Schneider RK, Puellen A, Kramann R, Raupach K, Bornemann J, Knuechel R, Pérez-Bouza A, Neuss S. The osteogenic differentiation of adult bone marrow and perinatal umbilical mesenchymal stem cells and matrix remodelling in three-dimensional collagen scaffolds. Biomaterials 2010;31:467-480.
24. Baksh D, Song L, Tuan R. Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med 2007;8:301-316.
25. Olivier V, Faucheux N, Hardouin P. Biomaterial challenges and approaches to stem cell use in bone reconstructive surgery. Drug Discov Today 2004;9:803-811.
26. Harley BA, Kim HD, Zaman MH, Yannas IV, Lauffenburger DA, Gibson LJ. Microarchitecture of three-dimensional scaffolds influences cell migration behavior viajunction interactions. Biophys J 2008; 95:4013-4024 | ||
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