| تعداد نشریات | 24 |
| تعداد شمارهها | 930 |
| تعداد مقالات | 8,355 |
| تعداد مشاهده مقاله | 16,846,425 |
| تعداد دریافت فایل اصل مقاله | 14,191,572 |
A developed recombinant antigen and monoclonal antibody based solid phase competitive ELISA for detection of foot and mouth disease virus serotype O specific antibodies | ||
| Iranian Journal of Veterinary Research | ||
| دوره 27، شماره 2 - شماره پیاپی 95، 2026، صفحه 180-186 اصل مقاله (686.15 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2026.53916.8090 | ||
| نویسندگان | ||
| S. Mallick* 1؛ R. P. Singh1؛ J. K. Biswal1؛ J. K. Mohapatra1؛ M. Rout1؛ S. K. Kabi2؛ R. Ranjan1 | ||
| 1ICAR-National Institute on Foot and Mouth Disease, Arugul, Bhubaneswar-752050, Odisha, India | ||
| 2MSc in Biotechnology, ICAR-National Institute on Foot and Mouth Disease, Arugul, Bhubaneswar-752050, Odisha, India | ||
| چکیده | ||
| Background: Foot and mouth disease (FMD) is ahighly contagious and economically burdensome viral disease of cloven-hoofed animals. Out of the seven FMD virus (FMDV) serotypes, type O is one of the most prevalent serotype in several parts of the world. Aims: This study aimed to develop a recombinant capsid polyprotein (rP1) and monoclonal antibody (MAb)-based solid phase competitive ELISA (rP1-MAb-SPCE) to detect anti-FMDV/O antibodies in serum samples. Methods: For the development of rP1-MAb-SPCE the concentrations of rP1, MAb and dilution of serum were optimized. Percent inhibition (PI) cut-off was determined based on ROC curve analysis of cattle sera (n=53) of known FMD vaccination status with reference to the gold standard virus neutralization test (VNT). The assay performance was further validated in comparison to the conventional polyclonal antibody (PAb)-based SPCE using FMDV-positive and negative serum samples (n=612) obtained from multiple species (cattle, goat, pig, fallow deer, antelope, giraffe and African buffalo). Results: At the selected cut-off value of 30.77 PI, the rP1-MAb-SPCE exhibited 86.21% diagnostic sensitivity and 95.83% diagnostic specificity. A significant correlation (r=0.84; P<0.0001) was observed between the PI values of sera tested by rP1-MAb-SPCE and PAb-based SPCE and also the limits of detection of both of the assays were comparable. Repeatability tests demonstrated that the coefficients of variation within and between runs were <10% and <14%, respectively. Conclusion: The developed rP1-MAb-SPCE has the potential to facilitate the detection of antibodies against FMDV serotype O while mitigating biosecurity concerns. However, further validation involving a larger serum cohort is essential to confirm the assay’s robustness. | ||
| کلیدواژهها | ||
| Foot and mouth disease virus؛ Monoclonal antibody؛ Recombinant protein؛ Serotype O؛ Solid phase competitive ELISA | ||
| مراجع | ||
|
Afroz, A; Verma, AK; Kumar, A; Upadhyay, S and Singh, A (2024). Detection of antibodies against structural proteins of foot-and-mouth disease virus in bovines of western Uttar Pradesh, India. Vet. Res. Forum. 15: 219-222.
Asfor, AS; Howe, N; Grazioli, S; Berryman, S; Parekh, K; Wilsden, G; Ludi, A; King, DP; Parida, S; Brocchi, E and Tuthill, TJ (2020). Detection of bovine antibodies against a conserved capsid epitope as the basis of a novel universal serological test for foot-and-mouth disease. J. Clin. Microbiol., 58: e01527-19.
Belsham, GJ (1993). Distinctive features of foot-and-mouth disease virus, a member of the picornavirus family; aspects of virus protein synthesis, protein processing and structure. Prog. Biophys. Mol. Biol., 60: 241-260.
Biswal, JK; Bisht, P; Mohapatra, JK; Ranjan, R; Sanyal, A and Pattnaik, B (2015). Application of a recombinant capsid polyprotein (P1) expressed in a prokaryotic system to detect antibodies against foot-and-mouth disease virus serotype O. J. Virol. Methods. 215-216: 45-51.
Cao, Y; Li, F; Xing, X; Zhang, H; Zhao, Q; Sun, P; Fu, Y; Li, P; Ma, X; Zhang, J; Zhao, Z; Yuan, H; Wang, J; Wang, T; Bao, H; Bai, X; Li, D; Zhang, Q; Li, K and Lu, Z (2025). Preparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease virus serotype O. Microbiol. Spectr., 13: e0223424-e0223437.
Chenard, G; Miedema, K; Moonen, P; Schrijver, RS and Dekker, A (2003). A solid-phase blocking ELISA for detection of type O foot-and-mouth disease virus antibodies suitable for mass serology. J. Virol. Methods. 107: 89-98.
Grubman, MJ and Baxt, B (2004). Foot-and-mouth disease. Clin. Microbiol. Rev., 17: 465-493.
ICAR-DFMD, Annual Report (2022). ICAR-Directorate of foot-and-mouth disease, Mukteswar, Nainital, Uttarakhand, India. P: 28.
Kang, YL; Jeong, JY; Choi, HY; Zhang, Y; Liu, Y; Lee, HJ; Choi, JC; Lee, SH; Lee, BJ; Lee, SW; Lee, JB; Cho, KH and Park, SY (2018). Evaluation and optimization of a conventional SPCE for FMD post-vaccination monitoring. BMC Vet. Res., 14: 371-378.
Kashem, MA; Sroga, P; Salazar, V; Amjad, H; Hole, K; Koziuk, J; Yang, M; Nfon, C and Babiuk, S (2024). Development and validation of serotype-specific blocking ELISA for the detection of anti-FMDV O/A/Asia1/SAT2 antibodies. Viruses. 16: 1438-1456.
Ko, YJ; Lee, HS; Park, JH; Lee, KN; Kim, SM; Cho, IS; Joo, HD; Paik, SG; Paton, DJ and Parida, S (2012). Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea. J. Virol. Methods. 179: 265-268.
Lee, HW; Yang, CY; Lee, MC; Chen, SP; Chang, HW and Cheng, IC (2022). The use of distinctive monoclonal antibodies in FMD VLP and P1-based blocking ELISA for the seromonitoring of vaccinated swine. Int. J. Mol. Sci., 23: 8542-8552.
Mackay, DK; Bulut, AN; Rendle, T; Davidson, F and Ferris, NP (2001). A solid-phase competition ELISA for measuring antibody to foot-and-mouth disease virus. J. Virol. Methods. 97: 33-48.
Mallick, S; Singh, RP; Biswal, JK; Mohapatra, JK; Rout, M; Samanta, R; Khulape, SA and Ranjan, R (2023). Production and characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and development of a sandwich ELISA for virus antigen detection. Vet. Res. Commun., 47: 1915-1924.
Mallick, S; Subramaniam, S; Biswal, JK; Ranjan, R; Mohapatra, JK and Sahoo, AP (2021). Short communication: preliminary observations on the serum levels of HSP70 and its correlation with serum cortisol, thyroid hormones, and acute-phase protein concentration in cattle naturally infected with foot-and-mouth disease virus. Trop. Aim. Health Prod., 53: 408-412.
OIE (Office International des Epizooties) (2008). Manual of diagnostic tests and vaccines for terrestrial animals. 6th Edn., OIE, Paris. PP: 121-133.
Paiba, GA; Anderson, J; Paton, DJ; Soldan, AW; Alexandersen, S; Corteyn, M; Wilsden, G; Hamblin, P; MacKay, DK and Donaldson, AI (2004). Validation of a foot-and-mouth disease antibody screening solid-phase competition ELISA (SPCE). J. Virol. Method. 115: 145-158.
Ran, X; Yang, Z; Bai, M; Zhang, Y; Wen, X; Guo, H and Sun, S (2019). Development and validation of a competitive ELISA based on bacterium-original virus-like particles of serotype O foot-and-mouth disease virus for detecting serum antibodies. Appl. Microbiol. Biotechnol., 103: 3015-3024.
Ranjan, R; Biswal, JK; Mallick, S and Sharma, AK (2025). Tlr-2 dependent and its associated cytokines expression profile in primo and revaccinated dairy cattle immunized with an inactivated foot-and-mouth disease vaccine. Explor. Anim. Med. Res., 15: 180-187.
Ranjan, R; Joshi, HR; Pandey, V; Biswal, JK; Das, S; Barik, B; Mohapatra, JK; Mallick, S and Singh, RP (2026). Kinetics of foot-and-mouth disease antibody response in cattle vaccinated with FMD-HS-BQ combined vaccine under field condition. Front. Microbiol., 17: 1800886-1800897.
Robiolo, B; La Torre, J; Duffy, S; Leon, E; Seki, C; Torres, A and Mattion, N (2010). Quantitative single serum-dilution liquid phase competitive blocking ELISA for the assessment of herd immunity and expected protection against foot-and-mouth disease virus in vaccinated cattle. J. Virol. Methods. 166: 21-27.
Sala, JM; Mansilla, FC; Miraglia, MC; Caspe, SG; Perez-Filgueira, DM and Capozzo, AV (2023). Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): Avidity ELISA as an alternative to the virus neutralization test. Front. Vet. Sci., 10: 1162477-1162486.
Şevik, Murat and Öztürk, FF (2013). Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses. Turk. J. Vet. Anim. Sci., 37: 523-528.
Subramaniam, S; Mohapatra, JK; Sahoo, NR; Sahoo, AP; Dahiya, SS; Rout, M; Biswal, JK; Ashok, KS; Mallick, S; Ranjan, R; Jana, C and Singh, RP (2022). Foot-and-mouth disease status in India during the second decade of the twenty-first century (2011-2020). Vet. Res. Commun., 46: 1011-1022.
World Organisation for Animal Health (2018). Manual of diagnostic tests and vaccines for terrestrial animals. (13th Edn.), WOAH, Paris. PP: 441-443. | ||
|
آمار تعداد مشاهده مقاله: 2 تعداد دریافت فایل اصل مقاله: 1 |
||