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Antimicrobial resistance profile and virulence gene prevalence in Listeria monocytogenes isolates from raw meat products | ||
| Iranian Journal of Veterinary Research | ||
| دوره 27، شماره 2 - شماره پیاپی 95، 2026، صفحه 161-169 اصل مقاله (343.46 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2026.52645.7857 | ||
| نویسندگان | ||
| M. Jamali Galeh1؛ E. Rahimi* 2؛ N. Zia Jahromi3 | ||
| 1Department of Food Hygiene, ShK.C., Islamic Azad University, Shahrekord, Iran | ||
| 2Department of Veterinary, ShK.C., Islamic Azad University, Shahrekord, Iran | ||
| 3Department of Biology, ShK.C., Islamic Azad University, Shahrekord, Iran | ||
| چکیده | ||
| Background: Human listeriosis epidemics are often related to the intake of contaminated food, particularly meat products. Aims: This study aimed to investigate the prevalence, molecular and phenotypic determination of antibiotic resistance and pathogenicity of Listeria monocytogenes in beef, sheep, goat, camel, and buffalo meat in Chaharmahal and Bakhtiari province. Methods: A total of 300 raw meat samples were obtained from retail outlets. Biochemical analysis revealed that 129 samples (43%) were contaminated with L. monocytogenes. Using species-specific PCR (16S rRNA gene amplification), all 129 isolates obtained from the original samples were identified as L. monocytogenes. In this study, actA1, hylA, iap, plcA, prfA, intlA, and intB genes were evaluated using multiplex PCR. Multiplex PCR amplification of aadB, aac(3')-IIa, penA, ermA, ermB, tetM, tetA, sulI, and sulII was performed. Results: The average number of L. monocytogenes isolates in sheep, cow, camel, goat, and buffalo meat was 40%. L. monocytogenes isolates derived from raw meat exhibited the highest resistance to β-lactam antibiotics. In addition, the lowest resistance to Trimethoprim-sulfamethoxazole was observed. After the hylA gene, the iap (44.18%) and actA1 (40.31%) genes had the greatest frequency across isolates from various meat sources. The genes penA, aadB, and aac(3')-IIa were found in most L. monocytogenes strains. The most identified genes among L. monocytogenes strains were penA, aadB, and aac(3)-IIa. Few strains were resistant to SXT, although penA was widely distributed (92.24%). Conclusion: In conclusion, L. monocytogenes isolates from raw meat exhibited a high prevalence of virulence genes and considerable resistance to multiple antibiotic classes. It is necessary to continuously track these features throughout the food chain to improve control measures and reduce public health risks. | ||
| کلیدواژهها | ||
| Antibiotic resistance؛ Listeria monocytogenes؛ Listeriosis؛ Meat products | ||
| مراجع | ||
|
Al Hulimi, A; Al Hulimi, A and Nossair, MA (2024). Detection of some virulence factors of Listeria species isolated from chicken and their products. Alex. J. Vet. Sci., 82: 74-83.
Amajoud, N; Leclercq, A; Soriano, JM; Bracq-Dieye, H; El Maadoudi, M; Senhaji, NS; Kounnoun, A; Moura, A; Lecuit, M and Abrini, J (2018). Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco. Food Control. 84: 436-441.
Arslan, S and Baytur, S (2019). Prevalence and antimicrobial resistance of Listeria species and subtyping and virulence factors of Listeria monocytogenes from retail meat. J. Food Saf., 39: e12578.
Baquero, F; Lanza, VF; Duval, M and Coque, TM (2020). Ecogenetics of antibiotic resistance in Listeria monocytogenes. Mol. Microbiol., 113: 570-579.
Barbosa, LN; Alves, FCB; Andrade, BFMT; Albano, M; Castilho, IG; Rall, VLM; Athayde, NB; Delbem, NLC; Roca, RdO and Júnior, AF (2014). Effects of Ocimum basilicum Linn essential oil and sodium hexa-metaphosphate on the shelf life of fresh chicken sausage. J. Food Prot., 77: 981-986.
Boukili, M; Filali, FR; Lafkih, N; Bouymajane, A; Sefiani, M and Moumni, M (2020). Prevalence, characterization and antimicrobial resistance of Listeria monocytogenes isolated from beef meat in Meknes city, Morocco. Germs. 10: 74-80.
Bouymajane, A; Filali, FR; Oulghazi, S; Lafkih, N; Ed-Dra, A; Aboulkacem, A; El Allaoui, A; Ouhmidou, B and Moumni, M (2021). Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods. Heliyon. 7:e06169.
Cavalcanti, AAC; Limeira, CH; de Siqueira, IN; de Lima, AC; de Medeiros, FJP; de Souza, JG; de Araújo Medeiros, NG; de Oliveira Filho, AA and de Melo, MA (2022). The prevalence of Listeria monocytogenes in meat products in Brazil: A systematic literature review and meta-analysis. Res. Vet. Sci., 145: 169-176.
Chen, A; Chen, X; Zheng, Z; Wang, H and Hu, M (2025). Virulence gene profiling and molecular typing of clinical isolates of Listeria monocytogenes from Wenzhou, Eastern China. Infect. Drug Resist., 18: 3957-3966.
Chen, M; Cheng, J; Zhang, J; Chen, Y; Zeng, H; Xue, L; Lei, T; Pang, R; Wu, S and Wu, H (2019). Isolation, potential virulence, and population diversity of Listeria monocytogenes from meat and meat products in China. Front. Microbiol., 10: 946.
D'agostino, M; Wagner, M; Vazquez-Boland, J; Kuchta, T; Karpiskova, R; Hoorfar, J; Novella, S; Scortti, M; Ellison, J and Murray, A (2004). A validated PCR-based method to detect Listeria monocytogenes using raw milk as a food model—towards an international standard. J. Food Prot., 67: 1646-1655.
Dapgh, AN and Salem, RL (2022). Molecular detection of Listeria monocytogenes in milk and some milk products. Int. J. Vet. Sci., 11: 514-519.
Duma, MN; Ciupescu, LM; Dan, SD; Crisan-Reget, OL and Tabaran, A (2024). Virulence and antimicrobial resistance of Listeria monocytogenes isolated from ready-to-eat food products in Romania. Microorganisms. 12: 954.
Gana, J; Gcebe, N; Moerane, R; Ngoshe, YB; Moabelo, K and Adesiyun, AA (2024). Detection of pathogenic serogroups and virulence genes in Listeria monocytogenes strains isolated from beef and beef products retailed in Gauteng Province, South Africa, using phenotypic and polymerase chain reaction (PCR)-based methods. Int. J. Microbiol., 2024: 8891963.
Gholami, A; Salehi, B and Jazi, FM (2019). The determination of the antimicrobial susceptibility and antimicrobial resistance gene patterns in L. monocytogenes. J. Med. Bacteriol., 8: 30-37.
Gray, JA; Chandry, PS; Kaur, M; Kocharunchitt, C; Bowman, JP and Fox, EM (2021). Characterisation of Listeria monocytogenes food-associated isolates to assess environmental fitness and virulence potential. Int. J. Food Microbiol., 350: 109247.
Hailu, T; Gugsa, G; Tsegaye, Y; Ahmed, M and Awol, N (2024). Occurrence and antimicrobial resistance pattern of Listeria monocytogenes isolated from bovine’s milk and meat in Mekelle City, Ethiopia. Adv. Public Health. 2024: 8813787.
Huang, C; Lu, TL and Yang, Y (2023). Mortality risk factors related to listeriosis—a meta-analysis. J. Infect. Public Health. 16: 771-783.
Ireton, K; Mortuza, R; Gyanwali, GC; Gianfelice, A and Hussain, M (2021). Role of internalin proteins in the pathogenesis of Listeria monocytogenes. Mol. Microbiol., 116: 1407-1419.
Ismaiel, AAR; Ali, AES and Enan, G (2014). Incidence of Listeria in Egyptian meat and dairy samples. Food Biotechnol., 23: 179-185.
Iwu, CD and Okoh, AI (2020). Characterization of antibiogram fingerprints in Listeria monocytogenes recovered from irrigation water and agricultural soil samples. PLoS One. 15: e0228956.
Jallewar, P; Kalorey, D; Kurkure, N; Pande, V and Barbuddhe, S (2007). Genotypic characterization of Listeria spp. isolated from fresh water fish. Int. J. Food Microbiol., 114: 120-123.
Kawacka, I and Olejnik-Schmidt, A (2024). High prevalence of virulence-associated genes and length polymorphism in actA and inlB genes identified in Listeria monocytogenes isolates from meat products and meat-processing environments in Poland. Pathogens. 13: 444.
Kawacka, I; Pietrzak, B; Schmidt, M and Olejnik-Schmidt, A (2023). Listeria monocytogenes isolates from meat products and processing environment in Poland are sensitive to commonly used antibiotics, with rare cases of reduced sensitivity to ciprofloxacin. Life. 13: 821.
Kayode, AJ and Okoh, AI (2022). Assessment of the molecular epidemiology and genetic multiplicity of Listeria monocytogenes recovered from ready-to-eat foods following the South African listeriosis outbreak. Sci. Rep., 12: 20129.
Komora, N; Bruschi, C; Magalhães, R; Ferreira, V and Teixeira, P (2017). Survival of Listeria monocytogenes with different antibiotic resistance patterns to food-associated stresses. Int. J. Food Microbiol., 245: 79-87.
Koopmans, MM; Brouwer, MC; Vázquez-Boland, JA and van de Beek, D (2023). Human listeriosis. Clin. Microbiol. Rev., 36: e00060-00019.
Law, JWF; Ab Mutalib, NS; Chan, KG and Lee, LH (2015). An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food [Review]. Front. Microbiol., 6: 1227.
Matle, I; Mbatha, KR and Madoroba, E (2020). A review of Listeria monocytogenes from meat and meat products: Epidemiology, virulence factors, antimicrobial resistance and diagnosis. Onderstepoort J. Vet. Res., 87: 1-20.
Maung, AT; Abdelaziz, MNS; Mohammadi, TN; Zhao, J; Mohamed, ET; Nakayama, M; Matsusita, K; Masuda, Y; Honjoh, Ki and Miyamoto, T (2023). Comparison of prevalence, characterization, antimicrobial resistance and pathogenicity of foodborne Listeria monocytogenes in recent 5 years in Japan. Microb. Pathog., 183: 106333.
Mejía, L; Espinosa-Mata, E; Freire, AL; Zapata, S and González-Candelas, F (2023). Listeria monocytogenes, a silent foodborne pathogen in Ecuador. Front. Microbiol., 14: 1278860.
Moabelo, KC; Gcebe, N; Gana, J; Ngoshe, YB and Adesiyun, AA (2023). Contamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africa. J. Food Saf., 43: e13055.
Mureddu, A; Mazza, R; Fois, F; Meloni, D; Bacciu, R; Piras, F and Mazzette, R (2014). Listeria monocytogenes persistence in ready-to-eat sausages and in processing plants. Ital. J. Food Saf., 3: 20-26.
Noll, M; Kleta, S and Al Dahouk, S (2018). Antibiotic susceptibility of 259 Listeria monocytogenes strains isolated from food, food-processing plants and human samples in Germany. J. Infect. Public Health. 11: 572-577.
Ollinger, J; Bowen, B; Wiedmann, M; Boor, KJ and Bergholz, TM (2009). Listeria monocytogenes σB modulates PrfA-mediated virulence factor expression. Infect. Immun., 77: 2113-2124.
Osman, KM; Zolnikov, TR; Samir, A and Orabi, A (2014). Prevalence, pathogenic capability, virulence genes, biofilm formation, and antibiotic resistance of Listeria in goat and sheep milk confirms need of hygienic milking conditions. Pathog. Glob. Health. 108: 21-29.
Panera-Martínez, S; Rodriguez-Melcon, C; Serrano-Galan, V; Alonso-Calleja, C and Capita, R (2022). Prevalence, quantification and antibiotic resistance of Listeria monocytogenes in poultry preparations. Food Control. 135: 108608.
Parra-Flores, J; Holý, O; Bustamante, F; Lepuschitz, S; Pietzka, A; Contreras-Fernández, A; Castillo, C; Ovalle, C; Alarcón-Lavín, MP and Cruz-Córdova, A (2022). Virulence and antibiotic resistance genes in Listeria monocytogenes strains isolated from ready-to-eat foods in Chile. Front. Microbiol., 12: 796040.
Pesavento, G; Ducci, B; Nieri, D; Comodo, N and Nostro, AL (2010). Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control. 21: 708-713.
Pombinho, R; Vieira, A; Camejo, A; Archambaud, C; Cossart, P; Sousa, S and Cabanes, D (2020). Virulence gene repression promotes Listeria monocytogenes systemic infection. Gut Microbes. 11: 868-881.
Ranjbar, R and Halaji, M (2018). Epidemiology of Listeria monocytogenes prevalence in foods, animals and human origin from Iran: a systematic review and meta-analysis. BMC Public Health. 18: 1-12.
Rippa, A; Bilei, S; Peruzy, MF; Marrocco, MG; Leggeri, P; Bossù, T and Murru, N (2024). Antimicrobial resistance of Listeria monocytogenes strains isolated in food and food-processing environments in Italy. Antibiotics. 13: 525.
Saytekin, AM; Adıguzel, A; Alkilani, K; Yucetepe, AG and Keskin, O (2025). Some virulence genes and biofilm formation capabilities of Listeria monocytogenes isolates from different sources. Ankara Univ. Vet. Fak. Derg., 72: 35-45.
Schiavano, GF; Guidi, F; Pomilio, F; Brandi, G; Salini, R; Amagliani, G; Centorotola, G; Palma, F; Felici, M and Lorenzetti, C (2023). Listeria monocytogenes strains persisting in a meat processing plant in Central Italy: Use of whole genome sequencing and in vitro adhesion and invasion assays to decipher their virulence potential. Microorganisms. 11: 1659.
Sharma, RK; Jalalpure, SS; Pathak, S; Ganapathy, S; Desvaux, M; Roy, S and Hegde, S (2024). Molecular detection of Listeria monocytogenes from different dairy and street food sources in North Karnataka, India. J. Infect. Public Health. 17: 696-703.
Shen, J; Zhang, G; Yang, J; Zhao, L; Jiang, Y; Guo, D; Wang, X; Zhi, S; Xu, X and Dong, Q (2022). Prevalence, antibiotic resistance, and molecular epidemiology of Listeria monocytogenes isolated from imported foods in China during 2018 to 2020. Int. J. Food Microbiol., 382: 109916.
Shonhiwa, AM; Ntshoe, G; Essel, V; Thomas, J and McCarthy, K (2019). A review of foodborne diseases outbreaks reported to the outbreak response unit, national institute for communicable diseases, South Africa, 2013-2017. Int. J. Infect. Dis., (Suppl 1), 79: 73.
Silva, A; Silva, V; Gomes, JP; Coelho, A; Batista, R; Saraiva, C; Esteves, A; Martins, Â; Contente, D and Diaz-Formoso, L (2024). Listeria monocytogenes from food products and food associated environments: Antimicrobial resistance, genetic clustering and biofilm insights. Antibiotics. 13: 447.
Srinivasan, V; Nam, H; Nguyen, L; Tamilselvam, B; Murinda, S and Oliver, S (2005). Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodborne Pathog. Dis., 2: 201-211.
Swetha, C; Porteen, K; Elango, A; Ronald, B; Senthil Kumar, T; Milton, A and Sureshkannan, S (2021). Genetic diversity, virulence and distribution of antimicrobial resistance among Listeria monocytogenes isolated from milk, beef, and bovine farm environment. Iran. J. Vet. Res., 22: 1-8.
Tahoun, AB; Abou Elez, RM; Abdelfatah, EN; Elsohaby, I; El-Gedawy, AA and Elmoslemany, AM (2017). Listeria monocytogenes in raw milk, milking equipment and dairy workers: Molecular characterization and antimicrobial resistance patterns. J. Glob. Antimicrob. Resist., 10: 264-270. Travier, L and Lecuit, M (2014). Listeria monocytogenes ActA: a new function for a ‘classic’ virulence factor. Curr. Opin. Microbiol., 17: 53-60.
Wieczorek, K; Dmowska, K and Osek, J (2012). Characterization and antimicrobial resistance of Listeria monocytogenes isolated from retail beef meat in Poland. Foodborne Pathog. Dis., 9: 681-685.
Wiśniewski, P; Zakrzewski, AJ; Zadernowska, A and Chajęcka-Wierzchowska, W (2022). Antimicrobial resistance and virulence characterization of Listeria monocytogenes strains isolated from food and food processing environments. Pathogens. 11: 1099.
Yehia, HM; Elkhadragy, MF; Aljahani, AH and Alarjani, KM (2020). Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat. Biosci. Rep., 40: BSR20201062.
Yoshikawa, Y; Ochiai, Y; Mochizuki, M; Fujita, O; Takano, T; Hondo, R and Ueda, F (2016). Genetic subtyping of Listeria monocytogenes via multiple-locus sequence typing using iap, sigB and actA. J. Vet. Med. Sci., 78: 1831-1839.
Zamuz, S; Munekata, PE; Dzuvor, CK; Zhang, W; Sant'Ana, AS and Lorenzo, JM (2021). The role of phenolic compounds against Listeria monocytogenes in food. A review. Trends Food Sci. Technol., 110: 385-392. | ||
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