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Mito-TEMPO improves preservation of cooled canine semen over a 72-hour period of storage | ||
| Iranian Journal of Veterinary Research | ||
| دوره 27، شماره 1 - شماره پیاپی 94، 2026، صفحه 28-37 اصل مقاله (653.86 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2026.52842.7905 | ||
| نویسندگان | ||
| J. Jafari1؛ H. Ghasemzadeh Nava* 2؛ A. Shahverdi3؛ V. Akbarinejad* 2 | ||
| 1Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 2Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 3Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran | ||
| چکیده | ||
| Background: The positive effect of Mito-TEMPO, as a mitochondria-targeted antioxidant, on semen preservation has been reported in various species; however, little information is available on canine semen in this regard. Aims: The present study was conducted to evaluate the effect of Mito-TEMPO on preservation of cooled canine semen over a 72 h period of storage. Methods: In study I, the effect of 0 (as control), 25, 50 and 100 μM concentrations of Mito-TEMPO were evaluated on sperm motility, morphology, viability and plasma membrane integrity at time points 0, 24, 48 and 72 h. In study II, the effect of 0 (as control) and 100 μM (as the most effective dose recognized in study I) concentration of Mito-TEMPO was assessed on reactive oxygen species (ROS) and mitochondrial membrane potential at time points 0 and 72 h. Results: In study I, motility, velocity, viability, and proportion of sperm with normal morphology and intact plasma membrane decreased in all groups over time (P<0.05), but this decrease was slower in sperm treated with different doses of Mito-TEMPO than untreated sperm (P<0.05). In this regard, decrease in total and progressive motility observed from 24 h onward in the untreated group; however, such decrease was detected form 48 h onward in groups treated with different doses of Mito-TEMPO (P<0.05). Although all doses of Mito-TEMPO were effective, treatment with 100 μM concentration of Mito-TEMPO provided the best preservation as compared with the control group (P<0.05). Furthermore, study II revealed that ROS generation increased and mitochondrial membrane potential decreased over time in both groups (P<0.05); however, treatment with 100 μM concentration of Mito-TEMPO ameliorated the increase in ROS and the decrease in mitochondrial membrane potential over time (P<0.05). Conclusion: Mito-TEMPO enhances structural and functional characteristics of canine sperm preserved at 4°C for 72 h, and could serve as an effective antioxidant in canine semen extender. | ||
| کلیدواژهها | ||
| Antioxidant؛ Canine sperm؛ Cooled preservation؛ Mito-TEMPO؛ Mitochondria | ||
| مراجع | ||
|
Aitken, RJ (2017). Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol. Reprod. Dev., 84: 1039-1052.
Aitken, RR; Whiting, S; De Iuliis, GN; McClymont, S; Mitchell, LA and Baker, MA (2012). Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase. J. Biol. Chem., 287: 33048-33060.
Akbarinejad, V; Fathi, R; Shahverdi, A; Esmaeili, V; Rezagholizadeh, A and Ghaleno, LR (2018). Activator of mitochondrial aldehyde dehydrogenase (Alda-1) could enhance quality of equine cooled semen by ameliorating loss of mitochondrial function over time. J. Equine Vet. Sci., 70: 63-70.
Akbarinejad, V; Fathi, R; Shahverdi, A; Esmaeili, V; Rezagholizadeh, A and Ghaleno, LR (2020). The relationship of mitochondrial membrane potential, reactive oxygen species, adenosine triphosphate content, sperm plasma membrane integrity, and kinematic properties in warmblood stallions. J. Equine Vet. Sci., 94: 103267.
Andersen, AH; Thinnesen, M; Failing, K and Goericke-Pesch, S (2018). Effect of reduced glutathione (GSH) supplementation to Tris-egg yolk extender on chilled semen variables of dogs. Anim. Reprod. Sci., 198: 145-153.
Asadzadeh, N; Abdollahi, Z; Esmaeilkhanian, S and Masoudi, R (2021). Fertility and flow cytometry evaluations of ram frozen semen in plant-based extender supplemented with Mito-TEMPO. Anim. Reprod. Sci., 233: 106836.
Baek, JI; Kim, YR; Lee, KY and Kim, UK (2023). Mitochondrial redox system: A key target of antioxidant therapy to prevent acquired sensorineural hearing loss. Front. Pharmacol., 14: 1176881.
Calabria, A; Del Prete, C; Roberto, C; Longobardi, V; Spada, S; Alfano, MT; De Felice, D; Gasparrini, B and Cocchia, N (2023). Effect of crocin supplementation in the extender on the quality of chilled canine semen. Anim. Reprod. Sci., 259: 107374.
Cassani, P; Beconi, MT and O'Flaherty, C (2005). Relationship between total superoxide dismutase activity with lipid peroxidation, dynamics and morphological parameters in canine semen. Anim. Reprod. Sci., 86: 163-173.
Davila, MP; Muñoz, PM; Bolaños, JM; Stout, TA; Gadella, BM; Tapia, JA; da Silva, CB; Ferrusola, CO and Peña, FJ (2016). Mitochondrial ATP is required for the maintenance of membrane integrity in stallion spermatozoa, whereas motility requires both glycolysis and oxidative phosphorylation. Reproduction, 152: 683-694.
Dutta, S; Sengupta, P; Roychoudhury, S; Chakravarthi, S; Wang, CW and Slama, P (2022). Antioxidant paradox in male infertility: 'A blind eye' on inflammation. Antioxidants (Basel), 11: 167.
Esmaeilkhanian, S; Asadzadeh, N and Masoudi, R (2023). Flow cytometry study of post-thawed buck spermatozoa: Mito-TEMPO improves cryopreservation performance by controlling apoptosis rate, DNA fragmentation and ROS production. Cryobiology. 110: 108-110.
Gaffney, EA; Ishimoto, K and Walker, BJ (2021). Modelling motility: The mathematics of spermatozoa. Front. Cell Dev. Biol., 9: 710825.
García-Vázquez, FA; Gadea, J; Matás, C and Holt, WV (2016). Importance of sperm morphology during sperm transport and fertilization in mammals. Asian J. Androl., 18: 844-850.
Gautier, C and Aurich, C (2022). "Fine feathers make fine birds" - The mammalian sperm plasma membrane lipid composition and effects on assisted reproduction. Anim. Reprod. Sci., 246: 106884.
Goericke-Pesch, S; Klaus, D; Failing, K and Wehrend, A (2012). Longevity of chilled canine semen comparing different extenders. Anim. Reprod. Sci., 135: 97-105.
Hassan, HA; Banchi, P; Domain, G; Vanderheyden, L; Prochowska, S; Nizański, W and Van Soom, A (2023). Mito-Tempo improves acrosome integrity of frozen-thawed epididymal spermatozoa in tomcats. Front. Vet. Sci., 10: 1170347.
Hollinshead, FK and Hanlon, DW (2017). Factors affecting the reproductive performance of bitches: a prospective cohort study involving 1203 inseminations with fresh and frozen semen. Theriogenology. 101: 62-72.
Javaheri Barfourooshi, H; Esmaeilkhanian, S; Dadashpour Davachi, A; Asadzadeh, N and Masoudi, R (2023). Effect of Mito-TEMPO on post-thawed semen quality in goats. Iran. J. Vet. Med., 17: 393-400.
Jiang, Q; Yin, J; Chen, J; Ma, X; Wu, M; Liu, G; Yao, K; Tan, B and Yin, Y (2020). Mitochondria-targeted antioxidants: A step towards disease treatment. Oxid. Med. Cell. Longev., 2020: 8837893.
Kowalczyk, A (2022). The role of the natural antioxidant mechanism in sperm cells. Reprod. Sci., 29: 1387-1394.
Koziorowska-Gilun, M and Strzezek, R (2011). Molecular forms of selected antioxidant enzymes in dog semen--electrophoretical identification. Pol. J. Vet. Sci., 14: 29-33.
Kumar, A; Kumar Ghosh, S; Katiyar, R; Gemeda, AE; Rautela, R; Bisla, A; Srivastava, N; Kumar Bhure, S; Devi, HL and Chandra, V (2022). Supplementation of Mito TEMPO and acetovanillone in semen extender improves freezability of buffalo spermatozoa. Andrology. 10: 775-788.
Kumar, L; Yadav, SK; Kushwaha, B; Pandey, A; Sharma, V; Verma, V; Maikhuri, JP; Rajender, S; Sharma, VL and Gupta, G (2016). Energy utilization for survival and fertilization-parsimonious quiescent sperm turn extravagant on motility activation in rat. Biol. Reprod., 94: 96.
Mahiddine, FY and Kim, MJ (2021). Overview on the antioxidants, egg yolk alternatives, and mesenchymal stem cells and derivatives used in canine sperm cryopreservation. Animals (Basel), 11: 1930.
Masoudi, R; Asadzadeh, N and Sharafi, M (2020). The mitochondria-targeted antioxidant Mito-TEMPO conserves rooster’s cooled semen quality and fertility potential. Theriogenology. 156: 236-241.
Masoudi, R; Asadzadeh, N and Sharafi, M (2021). Effects of freezing extender supplementation with mitochondria-targeted antioxidant Mito-TEMPO on frozen-thawed rooster semen quality and reproductive performance. Anim. Reprod. Sci., 225: 106671.
Michael, AJ; Alexopoulos, C; Pontiki, EA; Hadjipavlou-Litina, DJ; Saratsis, P; Ververidis, HN and Boscos, CM (2009). Effect of antioxidant supplementation in semen extenders on semen quality and reactive oxygen species of chilled canine spermatozoa. Anim. Reprod. Sci., 112: 119-135.
Mickelsen, WD; Memon, MA; Anderson, PB and Freeman, DA (1993). The relationship of semen quality to pregnancy rate and litter size following artificial insemination in the bitch. Theriogenology. 39: 553-560.
Miki, K (2007). Energy metabolism and sperm function. Soc. Reprod. Fertil. Suppl., 65: 309-325.
Moazamian, R; Polhemus, A; Connaughton, H; Fraser, B; Whiting, S; Gharagozloo, P and Aitken, RJ (2015). Oxidative stress and human spermatozoa: diagnostic and functional significance of aldehydes generated as a result of lipid peroxidation. Mol. Hum. Reprod., 21: 502-515.
Napolitano, G; Fasciolo, G and Venditti, P (2021). Mitochondrial management of reactive oxygen species. Antioxidants (Basel), 10: 1824.
O'Flaherty, C and Scarlata, E (2022). Oxidative stress and reproductive function: The protection of mammalian spermatozoa against oxidative stress. Reproduction. 164: F67-F78.
Peña, F; Núñez-Martínez, I and Morán, J (2006). Semen technologies in dog breeding: an update. Reprod. Domest. Anim., 41: 21-29.
Peña, FJ; Ortiz-Rodríguez, JM; Gaitskell-Phillips, GL; Gil, MC; Ortega-Ferrusola, C and Martín-Cano, FE (2022). An integrated overview on the regulation of sperm metabolism (glycolysis-krebs cycle-oxidative phosphorylation). Anim. Reprod. Sci., 246: 106805. Pizzimenti, S; Ciamporcero, E; Daga, M; Pettazzoni, P; Arcaro, A; Cetrangolo, G; Minelli, R; Dianzani, C; Lepore, A; Gentile, F and Barrera, G (2013). Interaction of aldehydes derived from lipid peroxidation and membrane proteins. Front. Physiol., 4: 242.
Roshan, NJ; Garoussi, MT and Akbarinejad, V (2023). Evaluation of the effect of melatonin implantation in rams and eCG dose in ewes synchronized by a CIDR-eCG protocol on reproductive performance of Lacaune sheep breed during non-breeding season. Anim. Reprod. Sci., 259: 107365.
Sugai, N; Werre, S; Cecere, JT and Balogh, O (2024). Comparing different sperm concentrations for optimizing cooled semen use in the dog. Front. Vet. Sci., 10: 1339840.
Tesi, M; Sabatini, C; Vannozzi, I; Di Petta, G; Panzani, D; Camillo, F and Rota, A (2018). Variables affecting semen quality and its relation to fertility in the dog: A retrospective study. Theriogenology. 118: 34-39.
Trnka, J; Blaikie, FH; Smith, RA and Murphy, MP (2008). A mitochondria-targeted nitroxide is reduced to its hydroxylamine by ubiquinol in mitochondria. Free Radic. Biol. Med., 44: 1406-1419.
Vieira, NMG; Losano, JDA; Angrimani, DSR; Kawai, GKV; Bicudo, LC; Rui, BR; da Silva, BDCS; Assumpção, MEOD and Nichi, M (2018). Induced sperm oxidative stress in dogs: Susceptibility against different reactive oxygen species and protective role of seminal plasma. Theriogenology. 108: 39-45.
Volpe, S; Leoci, R; Aiudi, G and Lacalandra, GM (2009). Relationship between motility and mitochondrial functional status in canine spermatozoa. Reprod. Domest. Anim., 44: 275-278.
Zarei, F; Daghigh-Kia, H and Masoudi, R (2022). Supplementation of ram’s semen extender with Mito-TEMPO II: Quality evaluation and flow cytometry study of post-thawed spermatozoa. Andrologia. 54: e14299.
Zarei, F; Kia, HD; Masoudi, R; Moghaddam, G and Ebrahimi, M (2021). Supplementation of ram’s semen extender with Mito-TEMPO I: Improvement in quality parameters and reproductive performance of cooled-stored semen. Cryobiology. 98: 215-218. | ||
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