Sustainable processing of seafood byproducts through enzymatic technologies: A comprehensive review

Ahmad, M and Benjakul, S (2010). Extraction and characterisation of pepsin-solubilised collagen from the skin of unicorn leatherjacket (Aluterus monocerous). Food Chem., 120: 817-824.

Alishahi, A and Aïder, M (2012). Applications of chitosan in the seafood industry and aquaculture: a review. Food Bioprocess Tech., 5: 817-830.

An, Y; Xiong, S; Liu, R; You, J; Yin, T and Hu, Y (2021). The effect of cross-linking degree on physicochemical properties of surimi gel as affected by MTGase. J. Sci. Food Agric., 101: 6228-6238.

Ando, H; Adachi, M; Umeda, K; Matsuura, A; Nonaka, M; Uchio, R and Motoki, M (1989). Purification and characteristics of a novel transglutaminase derived from microorganisms. Agric. Biol. Chem., 53: 2613-2617.

Aoki, H; Ahsan, MN; Matsuo, K; Hagiwara, T and Watabe, S (2004). Partial purification of proteases that are generated by processing of the Northern shrimp Pandalus borealis and which can tenderize beef. Int. J. Food Sci. Technol., 39: 471-480.

Armenta-López, R; Guerrero, IL and Huerta, S (2002). Astaxanthin extraction from shrimp waste by lactic fermentation and enzymatic hydrolysis of the carotenoprotein complex. J. Food Sci., 67: 1002-1006.

Arnesen, JA and Gildberg, A (2006). Extraction of muscle proteins and gelatine from cod head. Process Biochem., 41: 697-700.

Arvanitoyannis, IS and Kassaveti, A (2008). Fish industry waste: treatments, environmental impacts, current and potential uses. Int. J. Food Sci. Technol., 43: 726-745.

Balti, R; Barkia, A; Bougatef, A; Ktari, N and Nasri, M (2009). A heat-stable trypsin from the hepatopancreas of the cuttlefish (Sepia officinalis): Purification and characterisation. Food Chem., 113: 146-154.

Bekhit, AEA (2011). Fish roe: Fermentation. In: Heldman, DR; Hoover, DG and Wheeler, MB (Eds.), Encyclopedia of biotechnology in agriculture and food. Boca Raton, FL, USA: CRC Press. PP: 251-256.

Bekhit, AEDA (2022). Fish roe: Biochemistry, products, and safety. 1st Edn., London, UK: Academic Press. P: 413.

Benjakul, S; Klomklao, S and Simpson, BK (2009). Enzymes in fish processing. J. Food Technol., 11-235.

Binsi, PK and Shamasundar, BA (2012). Purification and characterization of transglutaminase from four fish species: Effect of added transglutaminase on the viscoelastic behaviour of fish mince. Food Chem., 132: 1922-1929.

Birschbach, P; Fish, N; Henderson, W and Willrett, D (2004). Enzymes: Tools for creating healthier and safer foods. Food Techol., 58: 20-24.

Bougatef, A (2013). Trypsins from fish processing waste: Characteristics and biotechnological applications—Comprehensive review. J. Clean. Prod., 57: 257-265.

Bougatef, A; Balti, R; Nasri, R; Jellouli, K; Souissi, N and Nasri, M (2010). Biochemical properties of anionic trypsin acting at high concentration of NaCl purified from the intestine of a carnivorous fish: Smooth hound (Mustelus mustelus). J. Agric. Food Chem., 58: 5763-5769.

Bustos, RO; Romo, OR and Hearly, M (1999). Purification of trypsin-like enzymes from Antarctic krill processing wastewater. Process Biochem., 35: 327-333.

Castillo-Yañez, FJ; Pacheco-Aguilar, R; Garcia-Carreño, FL and Navarrete-Del Toro, MA (2004). Characterization of acidic proteolytic enzymes from Monterey sardine viscera. Food Chem., 85: 343-350.

Chalamaiah, M; Dinesh Kumar, B; Hemalatha, B and Jyothimay, T (2012). Fish protein hydrolysates: Proximate composition, amino acid composition, antioxidant activities and applications: A review. Food Chem., 135: 3020-3028.

Chen, Y; Chen, J; Chen, J; Yu, H; Zheng, Y; Zhao, J and Zhu, J (2022). Recent advances in seafood bioactive peptides and their potential for managing osteoporosis. Crit. Rev. Food Sci. Nutr., 62: 1187-1203.

Chere, R; Delbarre-Ladrat, C; de Lamballerie-Anton, M and Verrez-Bagnis, V (2007). Calpain and cathepsin activities in post mortem fish and meat muscles. Food Chem., 101: 1474-1479.

Daboor, SM; Budge, SM; Ghaly, AE; Brooks, SL and Dave, D (2010). Extraction and purification of collagenase enzymes: A critical review. Am. J. Biochem. Biotechnol., 6: 239-263.

Daboor, SM; Budge, SM; Ghaly, AE and Dave, D (2012). Isolation and activation of collagenase from fish processing waste. Adv. Biosci. Biotechnol., 3: 191-203.

Dahiya, N; Tewari, R and Hoondal, GS (2006). Biotechnological aspects of chitinolytic enzymes: A review. Appl. Microbiol. Biotechnol., 71: 773-782.

Felberg, HS; Hagen, L; Sluppaug, G; Batista, I; Nunes, ML and Olen, R (2010). Partial characterization of gelatinolytic activities in herring (Clupea harengus) and sardine (Sardina pilchardus) possibly involved in post-mortem autolysis of ventral muscle. Food Chem., 119: 675-683.

Five, KK; Fålun, I; Roland, GJ; Forshaug, D; Helgeland-Rossavik, MK; Hals, R and Rustad, T (2024). Enzymatic hydrolysis of chicken viscera and bones: Rest raw material characterization and evaluation of industrially relevant process parameters on product yields. Process Biochem., 146: 68-80.

Food and Agriculture Organization of the United Nations (FAO) (2014). National Aquaculture Sector Overview – India. Rome, Italy: Food and Agriculture Organization of the United Nations (FAO). P: 13.

Gildberg, A (1993). Enzymic processing of marine raw materials. Process Biochem., 28: 1-15. https://doi.org/10. 1016/0032-9592(94)80030-8.

Guillén, M; Benaiges, MD and Valero, F (2011). Comparison of the biochemical properties of a recombinant lipase extract from Rhizopus oryzae expressed in Pichia pastoris with a native extract. Biochem. Eng. J., 54: 117-123.

Hannan, MA; Habib, KA; Shahabuddin, AM; Haque, MA and Munir, MB (2022). Processing of shrimp. Post-Harvest processing, packaging and inspection of frozen shrimp: A practical guide. Springer Nature Singapore, Singapore. PP: 59-77.

Hayes, M and Flower, D (2013). Bioactive peptides from marine processing byproducts. In: Hernández-Ledesma, B and Herrero, M (Eds.), Bioactive compounds from marine foods: plant and animal sources. John Wiley & Sons. PP: 57-71.

Hayes, M and Gallagher, M (2019). Processing and recovery of valuable components from pelagic blood-water waste streams: A review and recommendations. J. Clean. Prod., 215: 410-422.

He, H; Chen, X; Li, J; Zhang, Y and Gao, P (2004). Taste improvement of refrigerated meat treated with cold-adapted protease. Food Chem., 84: 307-311.

Hsieh, CW; Cannella, D; Jørgensen, H; Felby, C and Thygesen, LG (2014). Cellulase inhibition by high concentrations of monosaccharides. J. Agric. Food Chem., 62: 3800-3805.

Husain, Q (2010). β-Galactosidases and their potential applications: a review. Crit. Rev. Biotechnol., 30: 41-62.

Imm, JY and Lee, CM (1999). Production of seafood flavor from red hake (Urophycis chuss) by enzymatic hydrolysis. J. Agric. Food Chem., 47: 2360-2366.

Jung, WK; Park, PJ; Byun, HG and Kim, SK (2005). Preparation of hoki (Johnius belengeri) bone oligophosphopeptide with a high affinity to calcium by carnivorous intestine crude proteinase. Food Chem., 91: 333-337.

Kamal, MZ; Barrow, CJ and Rao, NM (2015). A computational search for lipases that can preferentially hydrolyze long-chain omega-3 fatty acids from fish oil triacylglycerols. Food Chem., 173: 1030-1036.

Kamal, H; Jafar, S; Mudgil, P; Murali, C; Amin, A and Maqsood, S (2018). Inhibitory properties of camel whey protein hydrolysates toward liver cancer cells, dipeptidyl peptidase-IV, and inflammation. J. Dairy Sci., 101: 8711-8720.

Kapoor, M and Gupta, MN (2012). Lipase promiscuity and its biochemical applications. Process Biochem., 47: 555-569.

Karim, AA and Bhat, R (2009). Fish gelatin: Properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocoll., 23: 563-576.

Khaled, HB; Ghorbel-Bellaaj, O; Hmidet, N; Jellouli, KJ and Ghorbel, S (2011). A novel aspartic protease from the viscera of sardinelle (Sardinella aurita): Purification and characterization. Food Chem., 128: 847-853.

Khandagale, AS; Sarojini, BS; Kumari, SN and Nooralabettu, K (2015). Isolation, purification, and biochemical characterization of trypsin from Indian mackerel (Rastrelliger kanagurta). J. Aquat. Food Prod. Technol., 24: 354-367.

Khangembam, BK and Chakrabarti, R (2015). Trypsin from the digestive system of carp (Cirrhinus mrigala): Purification, characterization and its potential application. Food Chem., 175: 386-394.

Khiari, Z (2024). Enzymes from fishery and aquaculture waste: Research trends in the era of artificial intelligence and circular bio-economy. Mar. Drugs. 22: 411-418.

Kim, SK and Mendis, E (2006). Bioactive compounds from marine processing by-products—A review. Int. Food Res., 39: 383-387.

Kim, SK and Rajapakse, N (2005). Enzymatic production and biological activities of chitosan oligosaccharides (COS): A review. Carbohydr. Polym., 62: 357-368.

Kim, KJ; Yang, YJ and Kim, JG (2003). Purification and characterization of chitinase from Streptomyces sp. M-20. BMB Rep., 36: 185-189.

Kishimura, H; Klomklao, S; Nalinanon, S; Benjakul, S and Adachi, K (2010). Comparative study on thermal stability of trypsin from the pyloric ceca of threadfin hakeling (Laemonema longipes). J. Food Biochem., 34: 50-65.

Klinkesorn, U; Kittikun, A; Chinachoti, P and Sophanodora, P (2004). Chemical transesterification of tuna oil to enriched omega-3 polyunsaturated fatty acids. Food Chem., 87: 415-421.

Klomklao, S; Benjakul, S and Simpson, BK (2012). Seafood enzymes: Biochemical properties and their impact on quality. In: Simpson, BK (Ed.), Food biochemistry and food processing. (2nd Edn.), Wiley-Blackwell. PP: 263-284.

Komai, T; Kawabata, C; Tojo, H; Gocho, S and Ichishima, E (2007). Purification of serine carboxypeptidase from the hepatopancreas of Japanese common squid (Todarodes pacificus) and its application for elimination of bitterness from bitter peptides. Fish. Sci., 73: 404-411.

Kristiansson, HG and Rasco, RA (2000). Fish protein hydrolyzates: Production, biochemical, and functional properties. Crit. Rev. Food Sci. Nutr., 40: 43-53.

Ktari, N; Fakhfakh, N; Balti, R; Khaled, HB; Nasri, N and Bougatef, A (2013). Effect of degree of hydrolysis and protease type on the antioxidant activity of protein hydrolysates from cuttlefish (Sepia officinalis) by-products. J. Aquat. Food Prod. Technol., 22: 436-448.

Kurtovic, I; Marshall, SN; Zhao, X and Simpson, BK (2009). Lipases from mammals and fishes. Rev. Fish Biol. Fish., 17: 18-40.

Lin, SB; Lin, YC and Chen, HH (2009). Low molecular weight chitosan prepared with the aid of cellulase, lysozyme and chitinase: Characterization and antibacterial activity. Food Chem., 116: 47-53.

Lopetcharat, K; Choi, YJ; Park, JW and Daeschel, MA (2001). Fish sauce products and manufacturing: A review. Food Rev. Int., 17: 65-88.

Murado, MA; del Pilar González, M and Vázquez, JA (2009). Recovery of proteolytic and collagenolytic activities from viscera by-products of rayfish (Raja clavata). Mar. Drugs. 7: 803-815.

Muzzarelli, RAA and Muzzarelli, C (2005). Chitosan chemistry: Relevance to the biomedical sciences. In: Heinze, T (Ed.), Polysaccharides I: Structure, characterisation and use. (1st Edn.), Advances in polymer science. Vol. 186, Berlin and Heidelberg, Germany: Springer-Verlag. PP: 151-209.

Nalinanon, S; Benjakul, S; Visessanguan, W and Kishimura, H (2008). Tuna pepsin: Characteristics and its use for collagen extraction from the skin of threadfin bream (Nemipterus spp.). J. Food Sci., 73: C413-C419.

Olsen, RL; Toppe, J and Karunasagar, I (2014). Challenges and realistic opportunities in the use of by-products from processing of fish and shellfish. Trends Food Sci. Technol., 36: 144-151.

Pathinathan, P; Arumugam, U; Elangovan, P; Nathan, F; Baboonsundaram, A and Antony, C (2024a). Dietary lentinan improves growth, haemato-biochemical indices, resistance to Streptococcus agalactiae infection and expression of immune genes in Asian seabass, Lates calcarifer. Aquaculture. 580: 740299-740306.

Pathinathan, P; Nathan, F; Baboonsundaram, A and Antony, C (2024b). Hematobiochemical changes, immunological response and immune gene expression in Nile tilapia (Oreochromis niloticus) experimentally infected with Shewanella putrefaciens. Indian J. Anim. Res., 1: 9-16.

Pathinathan, P; Nathan, F; Baboonsundaram, A and Antony, C (2024c). Levamisole enhances growth, haemato-biochemical indicators, resistance to Aeromonas veronii and expression of immune genes in Asian seabass, Lates calcarifer. Indian J. Anim. Res., 1: 9-15.

Peinado, I; Koutsidis, G and Ames, J (2016). Production of seafood flavour formulations from enzymatic hydrolysates of fish by-products. LWT—Food Sci. Technol., 66: 444-452.

Penven, A; Perez-Galvez, R and Bergé, JP (2013). By-products from fish processing: Focus on French industry. In: Pérez-Gálvez, R and Bergé, JP (Eds.), Utilization of fish waste. CRC Press. PP: 1-25.

Plekhova, NG; Chernenko, IN; Korolev, DV; Kozlovskaya, EP; Stepanyugina, AK; Shevchenko, OV and Shumatov, VB (2024). Evaluation of collagenase activity from crab hepatopancreas in different model systems. Bull. Exp. Biol. Med., 176: 457-460.

Prabhakar, S; Prabhakar, P; Nimesh, A and Banerjee, M (2025). Production and development of nutraceuticals using sustainable food system. In: Ashraf, SA and Adnan, M (Eds.), Food bioactives and nutraceuticals: dietary medicine for human health and well-beings. Springer Nature Singapore, Singapore. PP: 59-86.

Proespraiwong, P; Tassanakajon, A and Rimphanitchayakit, V (2010). Chitinases from the black tiger shrimp (Penaeus monodon): Phylogenetics, expression and activities. Comp. Biochem. Physiol. B Biochem. Mol. Biol., 156: 86-96.

Ray, AK; Ghosh, K and Ringø, E (2012). Enzyme-producing bacteria isolated from fish gut: A review. Aquac. Nutr., 18: 465-492.

Reppond, K; de Oliveira, ACM and Bechtel, PJ (2009). Enzymatic digestion of eye and brain tissues of sockeye and coho salmon and dusky rockfish commercially harvested in Alaska. J. Aquat. Food Prod. Technol., 18: 209-222.

Rigueto, CVT; de Oliveira, R; Gomes, KS; Alessandretti, I; Nazari, MT; Rosseto, M and Dettmer, A (2023). From waste to value-added products: A review of opportunities for fish waste valorization. Environ. Qual. Manag., 33: 203-221.

Rustad, T; Storrø, I and Slizyte, R (2011). Possibilities for the utilization of marine by-products. Int. J. Food Sci. Technol., 46: 2001-2014.

Sachindra, NM and Mahendrakar, NS (2015). Fish processing byproducts: Quality assessment and applications. Houston, TX, USA: Studium Press LLC.

Schulbach, KF; Johnson, JV; Simonne, AH; Kim, JM; Yagiz, Y and Marshall, MR (2013). Polyphenol oxidase inhibitor from blue mussel (Mytilus edulis) extract. J. Food Sci., 78: C425-C431.

Shahidi, F and Kamil, YVAJ (2001). Enzymes from fish and aquatic invertebrates and their application in the food industry. Trends Food Sci. Technol., 12: 435-464.

Sila, A; Nasri, R; Bougatef, A and Nasri, M (2012). Digestive alkaline proteases from the goby (Zosterisessor ophiocephalus): Characterization and potential application as detergent additive and in the deproteinization of shrimp wastes. J. Aquat. Food Prod. Technol., 21: 118-133.

Silva, JF; Espósito, TS; Marcuschi, M; Ribeiro, K; Cavalli, O and Oliveira, V (2011). Purification and partial characterisation of a trypsin from the processing waste of the silver mojarra (Diapterus rhombeus). Food Chem., 129: 777-782.

Sinha, AK; Kumar, V; Makkar, PS; De-Boeck, G and Becker, K (2011). Non-starch polysaccharides and their role in fish nutrition—A review. Food Chem., 127: 1409-1426.

Song, P; Zhang, X; Wang, S; Xu, W; Wang, F; Fu, R and Wei, F (2023). Microbial proteases and their applications. Front. Microbiol., 14: 1236368-1236375.

Sovik, SL and Rustad, T (2006). Effect of season and fishing ground on the activity of cathepsin B and collagenase in by-products from cod species. LWT—Food Sci. Technol., 39: 43-53.

Sriket, C (2014). Proteases in fish and shellfish: Role on muscle softening and prevention. Int. Food Res. J., 21: 433-445.

Stefansson, G (1998). Enzymes in the fishing industry. Food Techol., 42: 64-68.

Sutthiwanjampa, C and Kim, SM (2015). Production and characterization of hyaluronidase and elastase inhibitory protein hydrolysates from Venus clam. Nat. Prod. Res., 29: 1614-1623.

Taniguchi, AY and Takano, K (2004). Purification and properties of β-galactosidase from tilapia intestine. Fish. Sci., 70: 688-694.

Thiago, B; Cahu, B and Cordula, CR (2012). Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste. Process Biochem., 47: 570-577.

Toldra, F (2007). The role of muscle enzymes in dry-cured meat products with different drying conditions. Trends Food Sci. Technol., 17: 164-168.

Trincone, A (2011). Marine biocatalysts: Enzymatic features and applications. Mar. Drugs. 9: 478-499.

Tschersich, P and Choudhury, GS (1998). Arrowtooth flounder (Atheresthes stomias) protease as a processing aid. J. Aquat. Food Prod. Technol., 7: 77-89.

Vaishnav, A; Lal, J; Mehta, NK; Mohanty, S; Yadav, KK; Priyadarshini, MB and Singh, SK (2025). Unlocking the potential of fishery waste: Exploring diverse applications of fish protein hydrolysates in food and nonfood sectors. Environ. Sci. Pollut. Res., 20: 1-45.

Vazquez, JA; Amado, IR; Montemayor, M and Murado, MA (2013). Chondroitin sulfate, hyaluronic acid and chitin/chitosan production using marine waste sources: Characteristics, applications and eco-friendly processes: A review. Mar. Drugs, 11: 747-774.

Venugopal, V (2005). Application of enzymes in fish processing and quality control. In: Venugopal, V (Ed.), Seafood processing: Adding value through quick freezing, retortable packaging and cook-chilling. Chapter 14, Boca Raton, FL, USA: CRC Press.PP: 401-424.

Venugopal, V (2009). Seafood proteins: Nutritional value, bioactive peptides, marine and cold-adapted enzymes. In: Venugopal, V (Ed.), Marine products for healthcare: functional and bioactive nutraceutical compounds from the ccean. (1st Edn.), Boca Raton, FL, USA: CRC Press. PP: 103-142.

Venugopal, V (2011). Marine polysaccharides: Food applications. 1st Edn., Boca Raton, FL, USA: CRC Press. P: 396.

Venugopal, V (2016). Enzymes from seafood processing waste and their applications in seafood processing. Adv. Food Nutr. Res., 78: 47-69.

Venugopal, V; Lakshmanan, R; Doke, SN and Bongirwar, DR (2000). Enzymes in fish processing, biosensors and quality control. Food Biochem., 14: 21-74.

Venugopal Menon, V and Lele, SS (2015). Nutraceuticals and bioactive compounds from seafood processing waste. In: Kim, SK (Ed.), Springer handbook of marine biotechnology. (1st Edn.), Berlin and Heidelberg, Germany: Springer-Verlag. PP: 1405-1425.

Vercruysse, L; van Camp, J and Smagghe, G (2005). ACE inhibitory peptides derived from enzymatic hydrolyzates of animal muscle protein: A review. J. Agric. Food Chem., 53: 8106-8109.

Waffo, AFT; Mitrova, B; Tiedemann, K; Iobbi-Nivol, C; Leimkühler, S and Wollenberger, U (2021). Electrochemical trimethylamine N-oxide biosensor with enzyme-based oxygen-scavenging membrane for long-term operation under ambient air. Biosensors. 11: 98.

Yadav, KK; Gurawa, K; Sharma, S; Mohanty, S and Yadav, RP (2025). Understanding spoilage in canned fish products. Vigyan Varta. 6: 21-27.

Yang, F; Rustad, T; Xu, Y; Jiang, Q and Xia, W (2015). Endogenous proteolytic enzymes—A study of their impact on cod (Gadus morhua) muscle proteins and textural properties in a fermented product. Food Chem., 172: 551-558.

Zamani, A and Benjakul, S (2016). Trypsin from unicorn leatherjacket (Aluterus monoceros) pyloric caeca: Purification and its use for preparation of fish protein hydrolysate with antioxidative activity. J. Sci. Food Agric., 96: 962-969.

Zhang, L; Liu, Y; Lu, D; Han, J; Lu, X and Tian, Z (2015). Angiotensin converting enzyme inhibitory, antioxidant activities, and antihyperlipidaemic activities of protein hydrolysates from scallop mantle (Chlamys farreri). Int. J.
Food Prop., 18: 33-45.

Zhong, C; Cai, QF; Liu, GM; Sun, LC; Hara, K; Su, WJ and Cao, MJ (2012). Purification and characterisation of cathepsin L from the skeletal muscle of blue scad (Decapterus maruadsi) and comparison of its role with myofibril-bound serine proteinase in the degradation of myofibrillar proteins. Food Chem., 133: 1560-1568.

Zhu, BW; Zhao, JG; Yang, JF; Mikiro, T; Zhang, ZS and Zhou, DY (2008). Purification and partial characterization of a novel β-1,3-glucanase from the gut of sea cucumber (Stichopus japonicas). Process Biochem., 43: 1102-1106.