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Lactobacillus plantarum exopolysaccharides exert immunomodulatory function by regulating Th1 and Th2 responses in mice | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 4 - شماره پیاپی 93، 2025، صفحه 370-378 اصل مقاله (477.04 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.53669.8038 | ||
| نویسندگان | ||
| Q. Gong* 1؛ D. D. Fu1؛ M. Zhang1؛ J. W. Liu2؛ J. Y. Tian3 | ||
| 1Henan University of Science and Technology, Luoyang, China | ||
| 2MSc Student in Microbiology, Henan Engineering Research Center of Food Microbiology, Luoyang, China | ||
| 3MSc Student in Microbiology, Henan University of Science and Technology, Luoyang, China | ||
| چکیده | ||
| Background: Our previous study demonstrated that exopolysaccharides (EPS) from Lactobacillus plantarum (L. plantarum) act as an effective adjuvant. They can enhance both the immune response elicited by the oprH recombinant subunit vaccine against Pseudomonas aeruginosa (P. aeruginosa) and the protective efficacy in experimental animals. However, it is not clear whether oral administration of L. plantarum EPS can promote immune function in experimental animals. Aims: The present study aimed to explore the immunoregulatory function of L. plantarum EPS in mice. Methods: EPS was extracted from L. plantarum. EPS was then orally administered to the mice at doses of 20, 40, and 60 mg/kg for 20 consecutive days. After 10 and 20 days, the immune organ index, delayed-type hypersensitivity (DTH), phagocytic ability of peritoneal macrophages, serum hemolysin, and splenic lymphocyte proliferation of mice were measured. The concentrations of IgG1, IgG2a, IFN-γ, IL-2, IL-4, and IL-10 in serum were measured using ELISA. The percentage of CD4+ and CD8+ T lymphocytes subpopulations and the expression of transcription factors T-bet and GATA-3 in the mouse spleen cells were measured by flow cytometry and qRT-PCR, respectively. Results: L. plantarum EPS could induce high levels of immune organ index, DTH reaction, phagocytic ability of peritoneal macrophages, serum hemolysin, splenic lymphocyte proliferation, serum IgG1, IgG2a, Th1-type cytokines IL-2, IFN-γ, Th2-type cytokines IL-4, IL-10, CD4+ T lymphocyte, CD8+ T lymphocyte, T cell transcription factors T-bet and GATA-3, and has a certain dose-dependency. Conclusion: L. plantarum EPS can concurrently upregulate both Th1 and Th2 immune responses in mice, with this effect being more pronounced, particularly at the high dose. Our study provides a theoretical basis for research on L. plantarum EPS in veterinary. | ||
| کلیدواژهها | ||
| Exopolysaccharides؛ Immune indexes؛ Immunoregulatory function؛ Lactobacillus plantarum؛ Th1/Th2 immune response | ||
| مراجع | ||
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Abo-Saif, FAA and Sakr, EAE (2020). Characterization and bioactivities of exopolysaccharide produced from probiotic Lactobacillus plantarum 47FE and Lactobacillus pentosus 68FE. Bioact. Carbohydr. Diet. Fibre. 24: 100231.
Emamifar, A; Kadivar, M; Shahedi, M and Soleimanian-Zad, S (2011). Effect of nanocomposite packaging containing Ag and ZnO on inactivation of Lactobacillus plantarum in orange juice. Food Control. 22: 408-413.
Ganesan, N; Ronsmans, S and Hoet, P (2023). Comparing [3H] thymidine LPT and CFSE assay to assess lymphocyte proliferation in beryllium-exposed sarcoidosis patients. Heliyon. 9: e19242.
Gong, Q; Du, ZQ and Guo, JZ (2020). Study on immunoregulation function of peony seed proteolysis product in mice. J. Food Biochem., 44: e13353.
Gray, JP and Gardner, CR (2024). Hypersensitivity, delayed type. (4th Edn.), Encyclopedia of Toxicology, Netherlands, Amsterdam. PP: 439-442.
Hu, J; Park, JH and Kim, IH (2022). Effect of dietary supplementation with Lactobacillus plantarum on growth performance, fecal score, fecal microbial counts, gas emission and nutrient digestibility in growing pigs. Anim. Feed Sci. Technol., 290: 115295.
Huang, Y; Liang, W; Lu, Y; Xiong, J; Liu, D and Jia, X (2025). Identification, antioxidant and immunomodulatory activities of a neutral exopolysaccharide from Lactiplantibacillus plantarum DMDL 9010. Nutrients. 17: 2265.
Huang, Y; Wu, J; Wu, W; Lin, J; Liang, Y; Hong, Z; Jia, X and Liu, D (2022). Structural, antioxidant, and immunomodulatory activities of an acidic exopolysaccharide from Lactiplantibacillus plantarum DMDL 9010. Front. Nutr., 9: 1073071.
Jiang, Y and Yang, Z (2018). A functional and genetic overview of exopolysaccharides produced by Lactobacillus plantarum. J. Funct. Foods. 47: 229-240.
Kowsalya, M; Velmurugan, T; Mythili, R; Kim, W; Sudha, KG; Ali, S; Kalpana, B; Ramalingam, S and Rajeshkumar, MP (2023). Extraction and characterization of exopolysaccharides from Lactiplantibacillus plantarum strain PRK7 and PRK 11, and evaluation of their antioxidant, emulsion, and antibiofilm activities. Int. J. Biol. Macromol., 242: 124842.
Kudo, H; Miyanagaa, K and Yamamoto, N (2023). Immunomodulatory effects of extracellular glyceraldehyde 3-phosphate dehydrogenase of exopolysaccharide-producing Lactiplantibacillus plantarum JCM 1149. Food Funct., 14: 489.
Liu, J; Chen, N; Zhang, Z; Yang, M; Yang, Z; Du, W; Gu, X and Zhang, J (2024). Screening and evaluation of prebiotic exopolysaccharide of Lactobacillus plantarum on treating IBD in mice. Food. Biosci., 59: 104098.
Liu, Z; Wang, L; Gao, P; Yu, Y; Zhang, Y; Fotin, A; Wang, Q; Xu, Z; Wei, X; Fotina, T and Ma, J (2023). Salmonella Pullorum effector SteE regulates Th1/Th2 cytokine expression by triggering the STAT3/SOCS3 pathway that suppresses NF-κ B activation. Vet. Microbiol., 284: 109817.
Liu, Z; Zhang, Z; Qiu, L; Zhang, F; Xu, X; Wei, H and Tao, X (2017). Characterization and bioactivities of the exopolysaccharide from a probiotic strain of Lactobacillus plantarum WLPL04. J. Dairy Sci., 100: 6895-6950.
Maidana, LG; Gerez, J; Hohmann, MNS; Verri-Jr, WA and Bracarense, APFL (2021). Lactobacillus plantarum metabolites reduce deoxynivalenol toxicity on jejunal explants of piglets. Toxicon. 203: 12-21.
Naruszewicz, M; Johansson, ML; Zapolska-Downar, D and Bukowska, H (2002). Effect of Lactobacillus plantarum 299v on cardiovascular disease risk factors in smokers. Am. J. Clin. Nutr., 76: 1249-1255.
Neely, J; Reimers, A; Taylor, S; Masuda, M; Schroeder, S; Wright, B and Doyle, A (2022). GATA-3 and T-bet as diagnostic markers of non-esophageal eosinophilic gastrointestinal disease. J. Allergy Clin. Immunol., 149: AB203.
Pandey, Y; Panda, BSK; Kamboj, A; Alhussien, MN; Kapila, R and Dang, AK (2022). Macrophage-activating factor of bovine colostrum promotes phagocytic activity of murine macrophages and bovine phagocytes. J. Reprod. Immunol., 153: 103660.
Pradeepa; Shetty, AD; Matthews, K; Hegde, AR; Akshatha, B; Mathias, AB; Mutalik, S and Vidya, SM (2016). Multidrug resistant pathogenic bacterial biofilm inhibition by Lactobacillus plantarum exopolysaccharide. Bioact. Carbohydr. Diet. Fibre. 8: 7-14. Shang, X; Geng, L; Wei, H; Che, X; Xing, L; Xing, M; Xu, W and Li, JH (2024). Selenium-enriched Lactobacillus plantarum alleviate of high alkalinity-induced microbiota-gut-blood systems affect by improving the gut microbiota. Aquaculture. 593: 741294.
Srinoulprasert, Y (2021). Lymphocyte transformation test and cytokine detection assays: Determination of read out parameters for delayed-type drug hypersensitivity reactions. J. Immunol. Methods. 496: 113098.
Tian, JY; Gong, Q; Zhu, SJ and Li, YJ (2023). Extracellular polysaccharide of Lactobacillus plantarum enhance immune efficacy of oprH gene recombinant subunit vaccine from Pseudomonas aeruginosa. J. Vet. Med. Sci., 85: 1210-1215.
Wang, J; Wu, T; Fang, X and Yang, Z (2019). Manufacture of low-fat Cheddar cheese by exopolysaccharide-producing Lactobacillus plantarum JLK0142 and its functional properties. J. Dairy Sci., 102: 3825-3838.
Xing, R; Yang, H; Wang, X; Yu, H; Liu, S and Li, P (2020). Effects of calcium source and calcium level on growth performance, immune organ indexes, serum components, intestinal microbiota, and intestinal morphology of broiler chickens. J. Appl. Poult. Res., 29: 106-120.
Yue, F; Han, H; Xu, J; Yao, X; Qin, Y; Zhang, L; Sun, X; Huang, J; Zhang, F and Lü, X (2025). Effects of exopolysaccharides form Lactobacillus plantarum KX041 on high fat diet-induced gut microbiota and inflammatory obesity. Int. J. Biol. Macromol., 289: 138803.
Zhang, A; Zhang, Z; Zhang, K; Liu, X; Lin, X; Zhang, Z; Bao, T and Feng, Z (2021). Nutrient consumption patterns of Lactobacillus plantarum and their application in suancai. Int. J. Food Microbiol., 354: 109317.
Zhang, Q; Zhao, Q; Li, T; Lu, L; Wang, F; Zhang, H; Liu, Z; Ma, H; Zhu, Q; Wang, J; Zhang, X; Pei, Y; Liu, Q; Xu, Y; Qie, J; Luan, X; Hu, Z and Liu, X (2023). Lactobacillus plantarum-derived indole-3-lactic acid ameliorates colorectal tumorigenesis via epigenetic regulation of CD8+ T cell immunity. Cell Metab., 35: 943-960.
Zhang, Y; Zhang, C; Wang, J; Wen, Y; Li, H and Liu, X (2024). The investigation of soybean protein isolates and soybean peptides assisting Lactobacillus plantarum K25 to inhibit Escherichia coli. Curr. Res. Food Sci., 8: 100662.
Znazen, R; Kaabi, H; Hmida, S; Abid, HB; Tahar, SB; Zammit, I; Hafsia, A and Boukef, K (2006). Detection of serum hemolysins in autoimmune hemolytic anemia. Transfus. Clin. Biol., 13: 341-345. | ||
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