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The therapeutic effects of ovarian stromal stem cells in the natural ovarian aging process in rats | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 3 - شماره پیاپی 92، 2025، صفحه 298-308 اصل مقاله (1.12 M) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.52273.7805 | ||
| نویسندگان | ||
| G. Yildirim1؛ M. S. Unal2؛ F. Altinbasak* 3؛ S. Tan4 | ||
| 1Department of Stem Cell, Institute of Health Sciences, Pamukkale University, Denizli, Turkey | ||
| 2Department of Histology and Embryology, Faculty of Medicine, Pamukkale University, Denizli, Turkey | ||
| 3Department of Histology and Embryology, Faculty of Medicine, Hitit University, Corum, Turkey | ||
| 4Department of Histology and Embryology, Faculty of Medicine, Ordu University, Ordu, Turkey | ||
| چکیده | ||
| Background: Natural ovarian aging (NOA) is characterized by the age-dependent, gradual depletion of the primordial follicle reserve in the ovarian cortex, a decline in oocyte quality, and the potential to lead to infertility. Aims: This study investigated the effects of stromal stem cells (OSCs) isolated from the ovary on ovarian tissue and folliculogenesis by transplanting these cells into the ovaries of rats undergoing ovarian aging. Methods: Twelve 12–14-month-old Sprague Dawley rats with regular oestrous cycles and reduced oocyte counts were divided into control (n=6) and OSC transplantation (n=6) groups. Stromal stem cells isolated from 23–25-day-old female rat ovaries were injected into the OSC group. Follicle counts and ovarian morphology were assessed via hematoxylin-eosin (H&E) staining. Insulin-like Growth Factor 1 (IGF-1), Follicle-Stimulating Hormone Receptor (FSHR), and Anti-Müllerian Hormone (AMH) expression in ovarian tissue were analyzed by immunohistochemistry, while serum AMH, FSH, and estradiol (E2) levels were measured using enzyme-linked immunosorbent assay (ELISA). Results: After 30 days, ovarian function improved with increased ovarian reserve and tissue architecture showed marked improvement. IGF-1 and AMH expression were significantly higher in the OSC group than in controls (P=0.02), there was no statistically significant difference in FSHR expression between groups (P=0.09). Unlike AMH levels (P=0.5129), Serum E2 levels rose significantly in the OSC group (P=0.0001). Serum FSH levels were similar between the groups (P=0.444). Conclusion: These findings suggest that OSC transplantation may improve ovarian function and reserve in aged rats, presenting a potential regenerative treatment for natural ovarian aging. | ||
| کلیدواژهها | ||
| Infertility؛ Natural ovarian aging؛ Ovarian reserve؛ Ovarian stromal stem cells | ||
| مراجع | ||
|
Altinbasak, F; Unal, MS; Tan, S and Yildirim, G (2024). The effects of testicular stromal stem cells on surgically injured testicular tissue in rats. Anat. Histol. Embryol., 53: e13100.
Amanak, K; Karaöz, B and Sevil, Ü (2014). Effect of the modern life on infertility. TAF Preventive Medicine Bulletin. PP: 345-350.
Amanvermez, R and Tosun, M (2016). An update on ovarian aging and ovarian reserve tests. Int. J. Fertil. Steril., 9: 411-415.
Baker, J; Hardy, MP; Zhou, J; Bondy, C; Lupu, F; Bellvé, AR and Efstratiadis, A (1996). Effects of an Igf1 gene null mutation on mouse reproduction. Mol. Endocrinol., 10: 903-918.
Besikcioglu, HE; Sarıbas, GS; Ozogul, C; Tiryaki, M; Kilic, S; Pınarlı, FA and Gulbahar, O (2019). Determination of the effects of bone marrow derived mesenchymal stem cells and ovarian stromal stem cells on follicular maturation in cyclophosphamide induced ovarian failure in rats. Taiwan. J. Obstet. Gynecol., 58: 53-59.
Bezerra, MÉ; Barberino, R; Menezes, VG; Gouveia, BB; Macedo, TJ; Santos, JM; Monte, AP; Barros, VR and Matos, MH (2018). Insulin-like growth factor-1 (IGF-1) promotes primordial follicle growth and reduces DNA fragmentation through the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signalling pathway. Reprod. Fertil. Dev., 30: 1503-1513.
Casarini, L and Crépieux, P (2019). Molecular mechanisms of action of FSH. Front. Endocrinol., 10: 305.
Cruz, G; Fernandois, D and Paredes, AH (2017). Ovarian function and reproductive senescence in the rat: role of ovarian sympathetic innervation. Reproduction. 153: R59-R68.
Danilovich, N and Sairam, MR (2002). Haploinsufficiency of the follicle-stimulating hormone receptor accelerates oocyte loss inducing early reproductive senescence and biological aging in mice. Biol. Reprod., 67: 361-369.
Demeestere, I; Centner, J; Gervy, C; Englert, Y and Delbaere, A (2005). Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents. Reproduction. 130: 147-156.
Ding, C; Li, H; Wang, Y; Wang, F; Wu, H; Chen, R; Lv, J; Wang, W and Huang, B (2017). Different therapeutic effects of cells derived from human amniotic membrane on premature ovarian aging depend on distinct cellular biological characteristics. Stem Cell Res. Ther., 8: 1-14.
Ding, C; Zou, Q; Wang, F; Wu, H; Chen, R; Lv, J; Ling, M; Sun, J; Wang, W and Li, H (2018). Human amniotic mesenchymal stem cells improve ovarian function in natural aging through secreting hepatocyte growth factor and epidermal growth factor. Stem Cell Res. Ther., 9: 1-11.
Durdağ, GD and Berker, B (2008). Assessment of ovarian reserve. J. Clin. Obstet. Gynecol., 18: 254-265.
Durlinger, AL; Kramer, P; Karels, B; de Jong, FH; Uilenbroek, JTJ; Grootegoed, JA and Themmen, AP (1999). Control of primordial follicle recruitment by anti-Mullerian hormone in the mouse ovary. Endocrinology. 140: 5789-5796.
Elvin, JA and Matzuk, MM (1998). Mouse models of ovarian failure. Rev. Reprod., 3: 183-195.
Filip, S; Mokry, J; Horacek, J and English, D (2008). Stem cells and the phenomena of plasticity and diversity: a limiting property of carcinogenesis. Stem Cells Dev., 17: 1031-1038.
Guthrie, H; Garrett, W and Cooper, B (1998). Follicle-stimulating hormone and insulin-like growth factor-I attenuate apoptosis in cultured porcine granulosa cells. Biol. Reprod., 58: 390-396.
Hirakawa, T; Yotsumoto, F; Imi, S; Ito, N; Ishiwata, K; Nakabayashi, K; Watanabe, A; Shirasu, N; Urushiyama, D and Miyata, K (2024). Adipose-derived mesenchymal stem cell transplantation improves ovarian function and oocyte quality in aged mice. Anticancer Res., 44: 3577-3586.
Huang, B; Ding, C; Zou, Q; Lu, J; Wang, W and Li, H (2020). Human amniotic fluid mesenchymal stem cells improve ovarian function during physiological aging by resisting DNA damage. Front. Pharmacol., 11: 272.
Kwintkiewicz, J; Nishi, Y; Yanase, T and Giudice, LC (2010). Peroxisome proliferator-activated receptor-γ mediates bisphenol A inhibition of FSH-stimulated IGF-1, aromatase, and estradiol in human granulosa cells. Environ. Health Perspect., 118: 400-406.
Li, Z; Liu, Y; Tian, Y; Li, Q; Shi, W; Zhang, J; Zhang, H; Tan, Y; Yang, S and Yang, T (2023). Human umbilical cord mesenchymal stem cell-derived exosomes improve ovarian function in natural aging by inhibiting apoptosis. Int. J. Mol. Med., 52: 94.
Ling, L; Feng, X; Wei, T; Wang, Y; Wang, Y; Wang, Z; Tang, D; Luo, Y and Xiong, Z (2019). Human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation improves ovarian function in rats with premature ovarian insufficiency (POI) at least partly through a paracrine mechanism. Stem Cell Res. Ther., 10: 1-18.
Lobo, RA (2017). Hormone-replacement therapy: current thinking. Nat. Rev. Endocrinol., 13: 220-231.
Louhio, H; Hovatta, O; Sjöberg, J and Tuuri, T (2000). The effects of insulin, and insulin-like growth factors I and II on human ovarian follicles in long-term culture. Mol. Hum. Reprod., 6: 694-698.
Maheshwari, A; Fowler, P and Bhattacharya, S (2006). Assessment of ovarian reserve—should we perform tests of ovarian reserve routinely? Hum. Reprod., 21: 2729-2735.
Meczekalski, B; Czyzyk, A; Kunicki, M; Podfigurna-Stopa, A; Plociennik, L; Jakiel, G; Maciejewska-Jeske, M and Lukaszuk, K (2016). Fertility in women of late reproductive age: the role of serum anti-Müllerian hormone (AMH) levels in its assessment. J. Endocrinol. Invest., 39: 1259-1265.
Mehalko, K; Kim, M; Paye, S; Koh, K; Lu, RJ and Benayoun, BA (2023). Lack of accelerated ovarian aging in a follicle-stimulating hormone receptor haploinsufficiency model. Transl. Med. Aging. 7: 1-8.
Melnik, BC; John, SM and Schmitz, G (2011). Over-stimulation of insulin/IGF-1 signaling by western diet may promote diseases of civilization: lessons learnt from laron syndrome. Nutr. Metab., 8: 1-4.
Nakae, J; Kido, Y and Accili, D (2001). Distinct and overlapping functions of insulin and IGF-I receptors. Endocr. Rev., 22: 818-835.
Olooto, WE; Amballi, AA and Banjo, TA (2012). A review of female infertility; important etiological factors and management. J. Microbiol. Biotech. Res., 2: 379-385.
Parte, S; Bhartiya, D; Manjramkar, DD; Chauhan, A and Joshi, A (2013). Stimulation of ovarian stem cells by follicle stimulating hormone and basic fibroblast growth factor during cortical tissue culture. J. Ovarian Res., 6: 1-10.
Patel, H; Bhartiya, D; Parte, S; Gunjal, P; Yedurkar, S and Bhatt, M (2013). Follicle stimulating hormone modulates ovarian stem cells through alternately spliced receptor variant FSH-R3. J. Ovarian Res., 6: 1-15.
Peluso, C; Fonseca, F; Rodart, I; Cavalcanti, V; Gastaldo, G; Christofolini, D; Barbosa, C and Bianco, B (2014). AMH: An ovarian reserve biomarker in assisted reproduction. Clin. Chim. Acta., 437: 175-182.
Rahayu, S; Widhaningrum, SN; Saadah, M; Soewondo, A; Marhendra, APW; Djati, MS and Ciptadi, G (2018). The effects of powder of Ocimum canum Sims. leaves on ovary LHR expression and serum estradiol levels in premenopausal rats (Rattus norvegicus). AIP Conf. Proc., 7-8 March 2018, Malang City, Indonesia.
Randolph Jr, JF; Sowers, M; Bondarenko, IV; Harlow, SND; Luborsky, JL and Little, RJ (2004). Change in estradiol and follicle-stimulating hormone across the early menopausal transition: effects of ethnicity and age. J. Clin. Endocrinol. Metab., 89: 1555-1561.
Roson-Burgo, B; Sanchez-Guijo, F; Del Cañizo, C and De Las Rivas, J (2014). Transcriptomic portrait of human mesenchymal stromal/stem cells isolated from bone marrow and placenta. BMC Genomics. 15: 1-18.
Shin, EY; Jeong, S; Lee, JE; Jeong, DS; Han, DK; Hong, SH and Lee, DR (2024). Multiple treatments with human embryonic stem cell-derived mesenchymal progenitor cells preserved the fertility and ovarian function of perimenopausal mice undergoing natural aging. Stem Cell Res. Ther., 15: 58.
Volarevic, V; Bojic, S; Nurkovic, J; Volarevic, A; Ljujic, B; Arsenijevic, N; Lako, M and Stojkovic, M (2014). Stem cells as new agents for the treatment of infertility: current and future perspectives and challenges. Biomed Res. Int., 2014: 507234.
Wu, M; Guo, Y; Wei, S; Xue, L; Tang, W; Chen, D; Xiong, J; Huang, Y; Fu, F and Wu, C (2022). Biomaterials and advanced technologies for the evaluation and treatment of ovarian aging. J. Nanobiotechnol., 20: 374.
Zhang, J; Chen, Q; Du, D; Wu, T; Wen, J; Wu, M; Zhang, Y; Yan, W; Zhou, S and Li, Y (2019a). Can ovarian aging be delayed by pharmacological strategies? Aging (Albany NY). 11: 817-832.
Zhang, S; Huang, B; Su, P; Chang, Q; Li, P; Song, A; Zhao, X; Yuan, Z and Tan, J (2021). Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency. Stem Cell Res. Ther., 12: 1-16. Zhang, Q; Sun, J; Huang, Y; Bu, S; Guo, Y; Gu, T; Li, B; Wang, C and Lai, D (2019b). Human amniotic epithelial cell-derived exosomes restore ovarian function by transferring microRNAs against apoptosis. Mol. Ther. Nucleic Acids. 16: 407-418.
Zhang, Y; Xia, X; Yan, J; Yan, L; Lu, C; Zhu, X; Wang, T; Yin, T; Li, R and Chang, HM (2017). Mesenchymal stem cell-derived angiogenin promotes primodial follicle survival and angiogenesis in transplanted human ovarian tissue. Reprod. Biol. Endocrinol., 15: 1-12.
Zhu, L; Li, J; Xing, N; Han, D; Kuang, H and Ge, P (2015). American ginseng regulates gene expression to protect against premature ovarian failure in rats. Biomed. Res. Int., 2015: 767124. | ||
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