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Assessment of lecithin nanoliposomes addition to fresh low-density lipoprotein-based diluent for cold storage of ram semen | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 3 - شماره پیاپی 92، 2025، صفحه 270-277 اصل مقاله (261.98 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.51361.7626 | ||
| نویسندگان | ||
| R. Nahri1؛ M. Eslami* 2؛ F. Farrokhi-Ardabili3 | ||
| 1Graduated from Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| 2Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| 3Department of Animal Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| چکیده | ||
| Background: Maintaining the quality of collected semen is a crucial goal in assisted reproductive technologies. Aims: The present study aimed to evaluate the effect of lecithin nanoliposomes (LNLs) addition to purified low-density lipoproteins (LDL)-based extender on cold preservation of ram semen for up to 72 h. Methods: Ejaculates were collected from fertile rams, assessed, diluted with tris-citric acid-fructose-purified LDL extender, pooled and used within the experiment. Experimental groups supplemented with 0 (control), 1, 2, 4, and 8 mM, LNLs and stored at 4°C for 72 h. Kinematics, viability, DNA fragmentation index (DFI), membrane integrity (MI), malondialdehyde (MDA) and total nitrate nitrite (TNN) were assessed at 0, 24, 48 and 72 h. Results: Total motility, forward progressive motility, curvilinear velocity, average path velocity and straightness variables were improved by 1-8 mM LNLs addition (P<0.05). Greater viability (at 48 and 72 h) and MI (at 24, 48 and 72 h), and lower DFI (at 24, 48 and 72 h) were detected in LNLs treated groups compared with the control group (P<0.05). LNLs addition at 2-8 mM resulted in lower amounts of MDA and TNN at 48 and 72 h (P<0.05). Conclusion: LNLs were recommended as a protectant in ram semen tris-LDL-based diluent because they reduce peroxidative/nitrosative damage, fragmented DNA indices, and improve kinematics during cold preservation. | ||
| کلیدواژهها | ||
| DNA fragmentation؛ Lecithin nanoliposomes؛ Malondialdehyde؛ Ram؛ Spermatozoa | ||
| مراجع | ||
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