| تعداد نشریات | 24 |
| تعداد شمارهها | 935 |
| تعداد مقالات | 8,385 |
| تعداد مشاهده مقاله | 16,983,285 |
| تعداد دریافت فایل اصل مقاله | 14,372,263 |
Efficacy of enoxacin and levofloxacin against Theileria annulata schizont-infected cell line | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 3 - شماره پیاپی 92، 2025، صفحه 256-262 اصل مقاله (352.6 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.52200.7784 | ||
| نویسندگان | ||
| A. S. Prajapati* 1؛ H. H. Panchasara2؛ A. N. Suthar3؛ B. Das4؛ A. C. Patel5؛ S. H. Raval6؛ P. T. Sutaria7؛ K. J. Ankuya8؛ R. M. Patel3 | ||
| 1Division of Veterinary Medicine, Veterinary Clinical Complex (Veterinary Medicine), College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India | ||
| 2Livestock Research Station, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India | ||
| 3Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India | ||
| 4Department of Clinics (Veterinary Parasitology), College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India | ||
| 5Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India | ||
| 6Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India | ||
| 7Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India | ||
| 8Department of Livestock Production Management, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India | ||
| چکیده | ||
| Background: Theileriosis is a tick-borne disease that significantly hampers livestock industries in developing nations. Buparvaquone is the only available option for the management of theileriosis. On the other hand, drug use or organism evolution has led to the evolution of resistance over past twenty years. Treatment for theileriosis requires alternative therapeutic approaches. Aims: This work examined the in-vitro effectiveness of enoxacin and levofloxacin compounds against Theileria annulata cultures, targeting the topoisomerase II pathway of organism. Methods: The schizont infected cell line was established from infected cattle blood. The inhibitory effects of both drugs were assessed on T. annulata along with their cytotoxicity and hemolytic activity. Results: Enoxacin and levofloxacin efficiently inhibited 50% of the infection at 1,71,723 µM and 10,084 µM, respectively. Levofloxacin showed encouraging results during the experiment. For enoxacin and levofloxacin, the 50% cytotoxic effects were also measured at 10,529 µM and 10,116 µM, respectively. Enoxacin was safe on bovine red blood cells with an HC50 value of 26,49,031 µM compared to levofloxacin with 19,828 µM value. Conclusion: Levofloxacin showed satisfactory results compared to enoxacin. Further study is required along with other parameters to evaluate and assess the inhibitory activity of enoxacin and levofloxacin. | ||
| کلیدواژهها | ||
| Cytotoxicity؛ Enoxacin؛ In-vitro؛ Levofloxacin؛ Theileria annulata | ||
| مراجع | ||
|
Ali, Q; Zahid, O; Mhadhbi, M; Jones, B; Darghouth, MA; Raynes, G; Afshan, K; Birtles, R; Sargison, ND; Betson, M and Chaudhry, U (2022). Genetic characterisation of the Theileria annulata cytochrome b locus and its impact on buparvaquone resistance in bovine. Int. J. Parasitol. Drugs Drug Resist., 20: 65-75.
Batiha, GE; Beshbishy, AM; Tayebwa, DS; Adeyemi, OS; Yokoyama, N and Igarashi, I (2019). Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo. Trop. Med. Health. 47: 42-53.
Bork, S; Yokoyama, N; Ikehara, Y; Kumar, S; Sugimoto, C and Igarashi, I (2004). Growth-inhibitory effect of heparin on Babesia parasites. Antimicrob. Agents Chemother., 48: 236-241.
Buvanesvaragurunathan, K; Ganesh, J; Nagul Kumar, S; Porchezhiyan, V; Radha, A; Azhahianambi, P; Pandikumar, P and Ignacimuthu, S (2022). In vitro growth inhibitory effect of selected 18β-glycyrrhetinic acid esters on Theileria annulata. Exp. Parasitol., 236: 108258.
Chatanga, E; Mosssad, E; Abdo Abubaker, H; Amin Alnour, S; Katakura, K; Nakao, R and Salim, B (2019). Evidence of multiple point mutations in Theileria annulata cytochrome b gene incriminated in buparvaquone treatment failure. Acta Trop., 191: 128-132.
Cui, L; Mharakurwa, S; Ndiaye, D; Rathod, PK and Rosenthal, PJ (2015). Antimalarial drug resistance: Literature review and activities and findings of the ICEMR network. Am. J. Trop. Med. Hyg., 93: 57-68.
Edith, R; Harikrishnan, TJ; Ponnudurai, G; Gomathinayagam, S; Kumarasamy, P and Senthilkumar, TMA (2018). Molecular prevalence of Theileria annulata in cattle from different agroclimatic zones of Tamil Nadu, India. Int. J. Curr. Microbiol. Appl. Sci., 7: 2225-2231.
Fabrega, A; Madurga, S; Giralt, E and Vila, J (2009). Mechanism of action of and resistance to quinolones. Microb. Biotechnol., 2: 40-61.
Fief, CA; Hoang, KG; Phipps, SD; Wallace, JL and Deweese, JE (2019). Examining the impact of antimicrobial fluoroquinolones on human DNA topoisomerase IIα and IIβ. ACS Omega. 4: 4049-4055.
Garcia-Estrada, EC; Prada, CF; Fernandez-Rubio, C; Rojo-Vazquez, F and Balana-Fouce, R (2010). DNA topoisomerases in apicomplexan parasites: promising targets for drug discovery. Proc. Biol. Sci., 277: 1777-1787.
Gozalbes, R; Brun-Pascaud, M; Garcia-Domenech, R; Galvez, J; Girard, PM; Doucet, JP and Derouin, F (2000). Anti-toxoplasma activities of 24 quinolones and fluoroquinolones in vitro: prediction of activity by molecular topology and virtual computational techniques. Antimicrob. Agents Chemother., 44: 2771-2776.
Hayat, MF; Tigani, H; El, Amin; Hassan, SK; Shawgi, MS and Abdel Rahim, ME (2012). In vitro activity of the aqueous extract of Gardenia ternifolia fruits against Theileria lestoquardi. J. Med. Plants Res., 6: 5447-5451.
Heifetz, CL; Bien, PA; Cohen, MA; Dombrowski, ME; Griffin, TJ; Malta, TE; Sesnie, JC; Shapiro, MA and Wold, SA (1988). Enoxacin: in-vitro and animal evaluation as a parenteral and oral agent against hospital bacterial isolates. J. Antimicrob. Chemother., 21: 29-42.
Hooper, DC and Jacoby, GA (2016). Topoisomerase inhibitors: Fluoroquinolone mechanisms of action and resistance. Cold Spring Harb. Perspect. Med., 6: a025320-a025341.
Hostettler, I; Muller, J; Stephens, CE; Haynes, R and Hemphill, A (2014). A quantitative reverse-transcriptase PCR assay for the assessment of drug activities against intracellular Theileria annulata schizonts. Int. J. Parasitol. Drugs Drug Resist., 4: 201-209.
Hyde, JE (2007). Drug-resistant malaria - an insight. FEBS J., 274: 4688-4698.
Idowu, T and Schweizer, F (2017). Ubiquitous nature of fluoroquinolones: The oscillation between antibacterial and anticancer activities. Antibiotics. 6: 26-49.
Jalbrzykowska, K; Chrzanowska, A; Roszkowski, P and Struga, M (2022). The new face of a well-known antibiotic: A review of the anticancer activity of enoxacin and its derivatives. Cancers. 14: 3056-3078.
Keroack, CD; Elsworth, B and Duraisingh, MT (2019). To kill a piroplasm: genetic technologies to advance drug discovery and target identification in Babesia. Int. J. Parasitol., 49: 153-163.
Kohli, S; Atheya, UK and Thapliyal, A (2014). Prevalence of theileriosis in cross-bred cattle: Its detection through blood smear examination and polymerase chain reaction in Dehradun district, Uttarakhand, India. Vet. World. 7: 168-171.
Koning, HP (2017). Drug resistance in protozoan parasites. Emerg. Top. Life Sci., 1: 627-632.
Kundave, VR; Patel, AK; Patel, PV; Hasnani, JJ and Joshi, CG (2015). Detection of theileriosis in cattle and buffaloes by polymerase chain reaction. J. Parasit. Dis., 39: 508-513.
Lempereur, L; Larcombe, SD; Karagenc, ZDT; Bilgic, HB; Bakirci, S; Hacilarlioglu, S; Kinnaird, J; Thompson, J; Weir, W and Shiels, B (2017). Identification of candidate transmission blocking antigen genes in Theileria annulata and related vector-borne apicomplexan parasites. Genomics. 18: 438-451.
Levine, C; Hiasa, H and Marians, KJ (1998). DNA gyrase and topoisomerase IV: biochemical activities, physiological roles during chromosome replication, and drug sensitivities. Biochim. Biophys. Acta. 1400: 29-43.
Liu, J; Rashid, M; Wang, J; Liu, A; Guan, G; Li, Y; He, L; Yin, H and Luo, J (2020). Theileria annulata transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells. Ticks Tick Borne Dis., 11: 101365.
Lizundia, R; Werling, D; Langsley, G and Ralph, SA (2009). Theileria apicoplast as a target for chemotherapy. Antimicrob. Agents Chemother., 53: 1213-1217.
Mahmoudi, N; Ciceron, L; Franetich, JF; Farhati, K; Silvie, O; Eling, W; Sauerwein, R; Danis, M; Mazier, D and Derouin, F (2003). In vitro activities of 25 quinolones and fluoroquinolones against liver and blood stage Plasmodium spp. Antimicrob. Agents Chemother., 47: 2636-2639.
Mhadhbi, M; Chaouch, M; Ajroud, K; Darghouth, MA and BenAbderrazak, S (2015). Sequence polymorphism of cytochrome b gene in Theileria annulata tunisian isolates and its association with buparvaquone treatment failure. PloS One. 10: e0129678-e0129689.
Mhadhbi, M; Naouach, A; Boumiza, A; Chaabani, MF; BenAbderazzak, S and Darghouth, MA (2010). In vivo evidence for the resistance of Theileria annulata to buparvaquone. Vet. Parasitol., 169: 241-247.
O’Brien, J; Wilson, I; Orton, T and Pognan, F (2000). Investigation of the alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem., 267: 5421-5426.
Omar, MA; Salama, A; Elsify, A; Rizk, MA; Al-Aboody, MS; AbouLaila, M; El-Sayed, SA and Igarashi, I (2016). Evaluation of in vitro inhibitory effect of enoxacin on Babesia and Theileria parasites. Exp. Parasitol., 161: 62-67.
Prajapati, AS; Suthar, AN and Das, B (2019). Diagnosis and management of pseudo-pericarditis associated with theileriosis in a buffalo. Intas Polivet. 20: 86-87.
Prasanna, BA; Macha, V; Prajna, PK; Shalu, V and Srivastava A (2022). MMV560185 from pathogen box induces extrinsic pathway of apoptosis in Theileria annulata infected bovine leucocytes. Int. J. Parasitol. Drugs Drug Resist., 18: 20-31.
Ptaszynska, N; Gucwa, K; Olkiewicz, K; Heldt, M; Serocki, M; Stupak, A; Martynow, D; Debowski, D; Gitlin-Domagalska, A; Lica, J; Legowska, A; Milewski, S and Rolka, K (2020). Conjugates of ciprofloxacin and levofloxacin with cell-penetrating peptide exhibit antifungal activity and mammalian cytotoxicity. Int. J. Mol. Sci., 21: 4696-4721.
Sahoo, N; Behera, BK; Khuntia, HK and Dash, M (2017). Prevalence of carrier status theileriosis in lactating cows. Vet. World. 10: 1471-1474.
Salim, B; Chatanga, E; Jannot, G; Mossaad, E; Nakao, R and Weitzman, JB (2019). Mutations in the TaPIN1 peptidyl prolyl isomerase gene in Theileria annulata parasites isolated in Sudan. Int. J. Parasitol. Drugs Drug Resist., 11: 101-105.
Selim, AM; Das, M; Senapati, SK; Jena, GR; Mishra, C; Mohanty, B; Panda, SK and Patra, RC (2020). Molecular epidemiology, risk factors and hematological evaluation of asymptomatic Theileria annulata infected cattle in Odisha, India. Iran. J. Vet. Res., 21: 250-256.
Sharifiyazdi, H; Namazi, F; Oryan, A; Shahriari, R and Razavi, M (2012). Point mutations in the Theileria annulata cytochrome b gene is associated with buparvaquone treatment failure. Vet. Parasitol., 187: 431-435.
Singh, SK; Sudan, V; Sachan, P and Srivastava, A (2015). Salvage of Theileria infected calves with clinical manifestation of exophthalmia. J. Parasit. Dis., 39: 448-451.
Sinha, BS; Sarkar, S; Lodh, C; Gupta, AR; Batabyal, S and Jas, R (2021). Prevalence of bovine theileriosis in South Bihar. J. Pharm. Innov., 10: 776-780.
Song, M; Wu, H; Wu, S; Ge, T; Wang, G; Zhou, Y; Sheng, S and Jiang, J (2016). Antibiotic drug levofloxacin inhibits proliferation and induces apoptosis of lung cancer cells through inducing mitochondrial dysfunction and oxidative damage. Biomed. Pharmacother., 84: 1137-1143.
Suthar, A; Maji, C; Gopalkrishnan, A; Raval, SH; Kumar, R and Kumar, S (2021). Anti-piroplasmic activity of novobiocin as heat shock protein 90 inhibitor against in-vitro cultured Theileria equi and Babesia caballi parasites. Ticks Tick Borne Dis., 12: 101696-1016.
Tayebwa, DS; Tuvshintulga, B; Guswanto, A; Nugraha, AB; Batiha, GE; Gantuya, S; Rizk, MA; Vudriko, P; Sivakumar, T; Yokoyama, N and Igarashi, I (2018). The effects of nitidine chloride and camptothecin on the growth of Babesia and Theileria parasites. Ticks Tick Borne Dis., 9: 1192-1201.
Titus, EE; Palavesam, A; Rajaram, SM; Perumal, P; Darwin, SS; Sanmugapriya, NK; Janarthanam, G and Muthusamy, R (2023). In vitro efficacy of plumbagin and thymol against Theileria annulata. J. Parasit. Dis., 47: 152-160.
Tunitskaya, VL; Khomutov, AR; Kochetkov, SN; Kotovskaya, SK and Charushin, VN (2011). Inhibition of DNA gyrase by levofloxacin and related fluorine-containing heterocyclic compounds. Acta Naturae. 3: 94-99.
Valianatos, G; Valcikova, B; Growkova, K; Verlande, A; Mlcochova, J; Radova, L; Stetkova, M; Vyhnakova, M; Slaby, O and Uldrijan, S (2017). A small molecule drug promoting miRNA processing induces alternative splicing of MdmX transcript and rescues p53 activity in human cancer cells overexpressing MdmX protein. PloS One. 12: e0185801-18.
Velusamy, R; Ponnudurai, G; Alagesan, A; Rani, N; Kolte, SW and Rubinibala, B (2023). Epidemiology and molecular characterization of Theileria annulata in ticks collected from cattle in the central part of Tamil Nadu, India. J. Parasitol. Res., 122: 3077-3086.
Xie, L and Wang, DI (1994). Fed-batch cultivation of animal cells using different medium design concepts and feeding strategies. Biotechnol. Bioeng., 43: 1175-1189.
Yousef, S; Balkemy, F and El Damaty, H (2020). Mutations in Theileria annulata cytochrome B gene associated with buparvaquone resistance in cattle, Egypt. Pak. Vet. J., 6: 1-3. | ||
|
آمار تعداد مشاهده مقاله: 239 تعداد دریافت فایل اصل مقاله: 296 |
||