| تعداد نشریات | 24 |
| تعداد شمارهها | 917 |
| تعداد مقالات | 8,230 |
| تعداد مشاهده مقاله | 16,274,702 |
| تعداد دریافت فایل اصل مقاله | 13,751,925 |
SWEET transporter gene expression in barley during drought stress at the grain filling stage | ||
| Iran Agricultural Research | ||
| دوره 45، شماره 1، 2026، صفحه 1-7 اصل مقاله (701.5 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22099/iar.2025.51003.1628 | ||
| نویسندگان | ||
| Abbas Saidi* 1؛ Zohreh Hajibarat1؛ Mohammad Reza Ghaffari2؛ Ahmad Mosuapour Gorji3؛ Mehrshad Zienalabedini2؛ Habibollah Ghazvini4 | ||
| 1Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, I. R. Iran | ||
| 2Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, I. R. Iran | ||
| 3Department of Vegetable Research, Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, I. R. Iran | ||
| 4Seed and Plant Improvement Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, I. R. Iran. | ||
| چکیده | ||
| SWEET (Sugars Will Eventually be Exported Transporters) genes facilitate the mobilization of photosynthetic products from source leaves to sinks. They contribute to sucrose translocation and respond to abiotic stresses. This research examined the HvSWEET genes within the barley genome, focusing on their phylogenetic relationships, structural characteristics, chromosomal positions, and gene expression profiles in response to drought stress at 21 and 28 days post-anthesis (two important time points in seed filling). In silico analysis revealed 23 SWEET genes in barley, including information on chromosomal location, phylogenetic relationships, gene structures, conserved motifs, and cis-elements in promoter regions. Phylogenetic analysis grouped barley and maize SWEET sequences into five clusters. HvSWEET12/13c/14 genes contain multiple binding sites in their promoter regions, indicating involvement in multiple abiotic/biotic stress responses. Gene expression analysis showed up-regulation of HvSWEET7/12/13c/14 at 21 and 28 days after anthesis under drought conditions. The role of sucrose transport in grain filling at 21 and 28 days after anthesis in barley was highlighted. SWEET transporters influence source/sink relationships, presenting opportunities for genetic modification to enhance stress tolerance. These results offer valuable insights into the various functions of HvSWEET genes and highlight their potential application in enhancing barley resilience against stress. | ||
| کلیدواژهها | ||
| Barley؛ Cis-element؛ Gene expression؛ SWEET transporter gene | ||
| مراجع | ||
|
Asseng, S., & Van Herwaarden, A. F. (2003). Analysis of the benefits to wheat yield from assimilates stored prior to grain filling in a range of environments. Plant and Soil, 256, 217-229. https://doi.org/10.1023/A:1026231904221
Chen, L. Q., Hou, B. H., Lalonde, S., Takanaga, H., Hartung, M. L., Qu, X. Q., Guo, W. J., Kim, J. G., Underwood, W., Chaudhuri, B., & Frommer, W. B. (2010). Sugar transporters for intercellular exchange and nutrition of pathogens. Nature, 468(7323), 527-532. https://doi.org/10.1038/nature09606
Chen, L. Q., Qu, X. Q., Hou, B. H., Sosso, D., Osorio, S., Fernie, A. R., & Frommer, W. B. (2012). Sucrose efflux mediated by SWEET proteins as a key step for phloem transport. Science, 335(6065), 207-211. https://doi.org/10.1126/science.1213351
Durand, M., Porcheron, B., Hennion, N., Maurousset, L., Lemoine, R., & Pourtau, N. (2016). Water deficit enhances C export to the roots in Arabidopsis thaliana plants with contribution of sucrose transporters in both shoot and roots. Plant physiology, 170(3), 1460-1479. https://doi.org/10.1104/pp.15.01926
Eom, J. S., Chen, L. Q., Sosso, D., Julius, B.T., Lin, I. W., Qu, X. Q., Braun, D. M., & Frommer, W. B. (2015). SWEETs, transporters for intracellular and intercellular sugar translocation. Current opinion in plant biology, 25, 53-62. https://doi.org/10.1016/j.pbi.2015.04.005
Ferrandino, A., & Lovisolo, C. (2014). Abiotic stress effects on grapevine (Vitis vinifera L.): Focus on abscisic acid-mediated consequences on secondary metabolism and berry quality. Environmental and Experimental Botany, 103, 138-147. https://doi.org/10.1016/j.envexpbot.2013.10.012
Guo, W. J., Nagy, R., Chen, H. Y., Pfrunder, S., Yu, Y. C., Santelia, D., Frommer, W. B., & Martinoia, E. (2014). SWEET17, a facilitative transporter, mediates fructose transport across the tonoplast of Arabidopsis roots and leaves. Plant Physiology, 164(2), 777-789. https://doi.org/10.1104/pp.113.232751
Hajibarat, Z., Saidi, A., & Hajibarat, Z. (2018). Bioinformatics analysis of MADS-box in Brachypodium distachyon. Crop Biotechnology, 8(1), 1-15. (In Persian). https://doi.org/10.30473/cb.2018.4901
Hajibarat, Z., & Saidi, A. (2023). Filamentation temperature-sensitive (FtsH); key player in response to multiple environmental stress conditions and developmental stages in potato. Journal of Plant Growth Regulation, 42(7), 4223-4239. https://doi.org/10.1007/s00344-022-10885-x
Klemens, P. A., Patzke, K., Deitmer, J., Spinner, L., Le Hir, R., Bellini, C., Bedu, M., Chardon, F., Krapp, A., & Neuhaus, H. E. (2013). Overexpression of the vacuolar sugar carrier AtSWEET16 modifies germination, growth, and stress tolerance in Arabidopsis. Plant Physiology, 163(3), 1338-1352. https://doi.org/10.1104/pp.113.224972
Lemoine, R., Camera, S. L., Atanassova, R., Dédaldéchamp, F., Allario, T., Pourtau, N., Bonnemain, J. L., Laloi, M., Coutos-Thévenot, P., Maurousset, L., & Durand, M. (2013). Source-to-sink transport of sugar and regulation by environmental factors. Frontiers in Plant Science, 4, 272. https://doi.org/10.3389/fpls.2013.00272
Mathan, J., Singh, A., & Ranjan, A. (2021). Sucrose transport in response to drought and salt stress involves ABA‐mediated induction of OsSWEET13 and OsSWEET15 in rice. Physiologia Plantarum, 171(4), 620-637. https://doi.org/10.1111/ppl.13210
Nakashima, K., & Yamaguchi-Shinozaki, K. (2013). ABA signaling in stress-response and seed development. Plant Cell Reports, 32, 959-970. https://doi.org/10.1007/s00299-013-1418-1
Saidi, A., & Hajibarat, Z. (2020a). Computational study of environmental stress-related transcription factor binding sites in the promoter regions of maize auxin response factor (ARF) gene family. Notulae Scientia Biologicae, 12(3), 646-657. https://doi.org/10.15835/nsb12310823
Saidi, A., & Hajibarat, Z. (2020b). In-silico analysis of eukaryotic translation initiation factors (eIFs) in response to environmental stresses in rice (Oryza sativa). Biologia, 75(10), 1731-1738. https://doi.org/10.2478/s11756-020-00467-1
Sun, M. X., Huang, X. Y., Yang, J., Guan, Y. F., & Yang, Z. N. (2013). Arabidopsis RPG1 is important for primexine deposition and functions redundantly with RPG2 for plant fertility at the late reproductive stage. Plant Reproduction, 26, 83-91. https://doi.org/10.1007/s00497-012-0208-1
Yuan, M., Chu, Z., Li, X., Xu, C., & Wang, S. (2010). The bacterial pathogen Xanthomonas oryzae overcomes rice defenses by regulating host copper redistribution. The Plant Cell, 22(9), 3164-3176. https://doi.org/10.1105/tpc.110.078022
Yuan, M., & Wang, S. (2013). Rice MtN3/saliva/SWEET family genes and their homologs in cellular organisms. Molecular Plant, 6(3), 665-674. https://doi.org/10.1093/mp/sst035
| ||
|
آمار تعداد مشاهده مقاله: 199 تعداد دریافت فایل اصل مقاله: 313 |
||