| تعداد نشریات | 24 |
| تعداد شمارهها | 916 |
| تعداد مقالات | 8,202 |
| تعداد مشاهده مقاله | 15,918,331 |
| تعداد دریافت فایل اصل مقاله | 13,466,852 |
Investigating the effect of rosmarinic acid loaded magnetic nanoparticles against growth and biofilm formation of Staphylococcus aureus isolated from poultry meat | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 2 - شماره پیاپی 91، 2025، صفحه 161-169 اصل مقاله (719.14 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.51467.7647 | ||
| نویسندگان | ||
| A. Poursafar1؛ L. Asadpour* 2؛ M. Mokhtary3 | ||
| 1MSc Student in Microbiology, Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| 2Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| 3Department of Chemistry, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| چکیده | ||
| Background: Staphylococcus aureus, one of the causes of food poisoning, plays an important role in causing gastrointestinal inflammation. Aims: Given the spread of antibiotic resistance in S. aureus, the present study aimed to investigate the effect of rosmarinic acid (RA) loaded magnetic nanoparticles (Fe3O4NPs@RA) on inhibiting the growth and biofilm formation of S. aureus isolated from meat samples. Methods: Fe3O4NPs@RA have been synthesized and their antimicrobial activities were investigated against S. aureus isolated from poultry meat by broth micro-dilution. The anti-biofilm effect of these nanoparticles and their effect on the expression level of biofilm-associated genes were investigated using microplate and real-time PCR methods. The killing properties of Fe3O4NPs@RA against test bacteria investigated by time-kill assay. Results: The minimum inhibitory concentration (MIC) of Fe3O4NPs@RA against S. aureus isolates ranged from 31.2-125 µg/ml. Also, the treatment with a sub-MIC concentration of Fe3O4NPs@RA prevented the formation of biofilm by 50-82%, in different isolates and downregulated the expression level of icaA and icaD. Also, the treatment with the MIC concentration of Fe3O4NPs@RA caused a 2.4-fold decrease in the population of living bacteria after 4 h and the number of living bacteria decreased more than 99% after 8 h. In the cytotoxicity assay, during 48 h, Fe3O4NPs@RA had no cytotoxic effect on HEK-293 cells at concentrations lower than of 300 µg/ml. Conclusion: The results of the present study showed that Fe3O4NPs@RA were effective in inhibiting the growth and biofilm formation of S. aureus isolates and could be further investigated as an option for controlling S. aureus in food samples. | ||
| کلیدواژهها | ||
| Biofilm؛ Magnetic nanoparticles؛ Rosmarinic acid؛ Staphylococcus aureus | ||
| مراجع | ||
|
Aksamija, A; Polidori, A; Plasson, R; Dangles, O and Tomao, V (2016). The inclusion complex of rosmarinic acid into beta-cyclodextrin: A thermodynamic and structural analysis by NMR and capillary electrophoresis. Food Chem., 208: 258-263.
Al-Hunaiti, A; Zihlif, M; Abu Thiab, T; Al-Awaida, W; Al-Ameer, HJ and Imraish, A (2024). Magnetic nanoparticle-based combination therapy: Synthesis and in vitro proof of concept of CrFe2O4-rosmarinic acid nanoparticles for anti-inflammatory and antioxidant therapy. PLoS One. 19: e0297716.
Bakkali, F; Averbeck, S; Averbeck, D and Idaomar, M (2008). Biological effects of essential oils - a review. Food Chem. Toxicol., 46: 446-475.
Cornell, RM and Schwertmann, U (2000). The iron oxides: structure, properties, reactions, occurrences and uses. 2nd Edn., Darmstadt, Germany, Wiley. PP: 1-8.
da Silva, SB; Ferreira, D; Pintado, M and Sarmento, B (2016). Chitosan-based nanoparticles for rosmarinic acid ocular delivery—in vitro tests. Int. J. Biol. Macromol., 84: 112-120.
Fahimirad, S; Abtahi, H; Razavi, SH; Alizadeh, H and Ghorbanpour, M (2017). Production of recombinant antimicrobial polymeric protein beta casein-E 50-52 and its antimicrobial synergistic effects assessment with thymol. Molecules. 22: 822-838.
Ghadimi, N; Asadpour, L and Mokhtary, M (2025). Enhanced antimicrobial, anti-biofilm, and efflux pump inhibitory effects of ursolic acid-conjugated magnetic nanoparticles against clinical isolates of multidrug-resistant Pseudomonas aeruginosa. Microb. Pathog., 199: 107241-107250.
Gyawali, R and Ibrahim, SA (2014). Natural products as antimicrobial agents. Food Control. 46: 412-429.
Harindranath, H; Susil, A; Rajeshwari, S; Sekar, M and Kumar, BP (2024). Unlocking the potential of rosmarinic acid: A review on extraction, isolation, quantification, pharmacokinetics and pharmacology. Phytomed. Plus., 29: 100726-.
Huang, J; Chen, PX; Rogers, MA and Wettig, SD (2019). Investigating the phospholipid effect on the bioaccessibility of rosmarinic acid-phospholipid complex through a dynamic gastrointestinal in vitro model. Pharmaceutics. 11: 156-174.
Ivanov, M; Kostić, M; Stojković, D and Soković, M (2022). Rosmarinic acid-modes of antimicrobial and antibiofilm activities of a common plant polyphenol. S. Afr. J. Bot., 146: 521-527.
Kernou, ON; Azzouz, Z; Madani, K and Rijo, P (2023). Application of rosmarinic acid with its derivatives in the treatment of microbial pathogens. Molecules. 28: 4243-4263.
Klancnik, A; Piskernik, S; Jersek, B and Smole Mozina, S (2010). Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. J. Microbiol. Methods. 81: 121-126.
Kotulova, D and Slobodnikova, L (2010). Susceptibility of Staphylococcus aureus biofilms to vancomycin, gentamicin and rifampin. Epidemiol. Microbiol. Immunol., 59: 80-87.
Laurent, S; Saei, AA; Behzadi, S; Panahifar, A and Mahmoudi, M (2014). Superparamagnetic iron oxide nanoparticles for delivery of therapeutic agents: opportunities and challenges. Expert Opin. Drug Deliv., 11: 1449-1470.
Le, UC; Mai, NX; Le, KM; Vu, HA; Tran, HN and Doan, TL (2024). Development and evaluation of rosmarinic acid loaded novel fluorescent porous organosilica nanoparticles as potential drug delivery system for cancer treatment. Arab. J. Chem., 17: 105402-105411.
Liang, T; Liang, Z; Wu, S; Ding, Y; Wu, Q and Gu, B (2023). Global prevalence of Staphylococcus aureus in food products and its relationship with the occurrence and development of diabetes mellitus. Med. Adv., 1: 53-78.
Madureira, AR; Campos, DA; Oliveira, A; Sarmento, B; Pintado, MM and Gomes, AM (2016). Insights into the protective role of solid lipid nanoparticles on rosmarinic acid bioactivity during exposure to simulated gastrointestinal conditions. Colloids Surf. B., 139: 277-284.
Marques, VF; Santos, HA; Santos, TH; Melo, DA; Coelho, SM; Coelho, IS and Souza, M (2021). Expression of icaA and icaD genes in biofilm formation in Staphylococcus aureus isolates from bovine subclinical mastitis. Pesq. Vet. Bras., 41: e06645.
Martínez, A; Manrique-Moreno, M; Klaiss-Luna, MC; Stashenko, E; Zafra, G and Ortiz, C (2021). Effect of essential oils on growth inhibition, biofilm formation and membrane integrity of Escherichia coli and Staphylococcus aureus. Antibiotics. 10: 1474-1489.
McKay, GA; Beaulieu, S; Arhin, FF; Belley, A; Sarmiento, I; Parr, JrT and Moeck, G (2009). Time-kill kinetics of oritavancin and comparator agents against Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium. J. Antimicrob. Chemother., 63: 1191-1199.
Mencherini, T; Picerno, P; Scesa, C and Aquino, R (2007). Triterpene, antioxidant, and antimicrobial compounds from Melissa officinalis. J. Nat. Prod., 70: 1889-1894.
Nadeem, M; Imran, M; Aslam Gondal, T; Imran, A; Shahbaz, M; Muhammad Amir, R; Wasim Sajid, M; Batool Qaisrani, T; Atif, M; Hussain, G and Salehi, B (2019). Therapeutic potential of rosmarinic acid: A comprehensive review. J. Appl. Sci., 9: 3139-3161.
Pal, M; Ketchakmadze, D; Durglishvili, N and Ketchakmadze, K (2022). Staphylococcus aureus: A major pathogen of food poisoning: A rare research report. Nutr. Food Process., 5: 1-3.
Park, J; Rho, SJ and Kim, YR (2019). Enhancing antioxidant and antimicrobial activity of carnosic acid in rosemary (Rosmarinus officinalis L.) extract by complexation with cyclic glucans. Food Chem., 299: 125119-125129.
Paseban, K; Noroozi, S; Gharehcheloo, R; Haddadian, A; Robattorki, FF; Dibah, H; Amani, R; Sabouri, F; Ghanbarzadeh, E; Hajrasouiha, S and Azari, A (2024). Preparation and optimization of niosome encapsulated meropenem for significant antibacterial and anti-biofilm activity against methicillin-resistant Staphylococcus aureus isolates. Heliyon. 10: e35651. Petersen, M (2013). Rosmarinic acid: new aspects. Phytochem. Rev., 12: 207-227.
Raeisi, M; Tabaraei, A; Hashemi, M and Behnampour, N (2016). Effect of sodium alginate coating incorporated with nisin, Cinnamomum zeylanicum, and rosemary essential oils on microbial quality of chicken meat and fate of Listeria monocytogenes during refrigeration. Int. J. Food Microbiol., 238: 139-145.
Rama Devi, K; Srinivasan, R; Kannappan, A; Santhakumari, S; Bhuvaneswari, M; Rajasekar, P; Prabhu, NM and Veera Ravi, A (2016). In vitro and in vivo efficacy of rosmarinic acid on quorum sensing mediated biofilm formation and virulence factor production in Aeromonas hydrophila. Biofouling. 32: 1171-1183.
Romano, A; Carrella, S; Rezza, S; Nia, Y; Hennekinne, JA; Bianchi, DM; Martucci, F; Zuccon, F; Gulino, M; Di Mari, C and Zaccaria, T (2023). First report of food poisoning due to staphylococcal enterotoxin type B in Döner Kebab (Italy). Pathogens. 12: 1139-1150.
Scandorieiro, S; Rodrigues, BC; Nishio, EK; Panagio, LA; De Oliveira, AG; Durán, N; Nakazato, G and Kobayashi, RK (2022). Biogenic silver nanoparticles strategically combined with Origanum vulgare derivatives: Antibacterial mechanism of action and effect on multidrug-resistant strains. Front. Microbiol., 13: 842600-84626.
Shafiei, M; Ali, AA; Shahcheraghi, F; Saboora, A and Noghabi, KA (2014). Eradication of Pseudomonas aeruginosa biofilms using the combination of n-butanolic cyclamen coum extract and ciprofloxacin. Jundishapur J. Microbiol., 7: e14358.
Slobodníková, L; Fialová, S; Hupková, H and Grančai, D (2013). Rosmarinic acid interaction with planktonic and biofilm Staphylococcus aureus. Nat. Prod. Commun., 8: 1747-1750.
Tiwari, RP; Bharti, SK; Kaur, HD; Dikshit, R and Hoondal, GS (2005). Synergistic antimicrobial activity of tea and antibiotics. Indian J. Med. Res., 122: 180-186.
Wagnera, H and Ulrich-Merzenich, G (2009). Synergy research: Approaching a new generation of phyto-pharmaceuticals. Phytomedicine. 16: 97-110.
Wang, D; Wang, L; Liu, Q and Zhao, Y (2025). Virulence factors in biofilm formation and therapeutic strategies for Staphylococcus aureus: A review. Anim. Zoonoses., 1: 188-202.
Xie, J; VanAlstyne, P; Uhlir, A and Yang, X (2017). A review on rosemary as a natural antioxidation solution. Eur. J. Lipid Sci. Technol., 119: 1600439.
Yang, JH; Mao, KJ; Huang, P; Ye, YJ; Guo, HS and Cai, BC (2018). Effect of piperine on the bioavailability and pharmacokinetics of rosmarinic acid in rat plasma using UPLC-MS/MS. Xenobiotica. 48: 178-185. | ||
|
آمار تعداد مشاهده مقاله: 264 تعداد دریافت فایل اصل مقاله: 207 |
||