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Molecular detection and genotyping of Theileria equi infection within the equine population in Giza, Egypt, using real-time PCR as compared with conventional detection methods | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 2 - شماره پیاپی 91، 2025، صفحه 145-151 اصل مقاله (444.24 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.51028.7553 | ||
| نویسندگان | ||
| A. M. Soliman* 1؛ N. M. Elhawary2؛ N. M. Helmy1؛ M. A. El-Seify2؛ M. M. Amer1؛ S. Mohamed3؛ F. U. Memon4؛ M. H. O. Rashid5؛ S. M. Gadelhaq6 | ||
| 1Biotechnology Department, Animal Health Research Institute, Agricultural Research Center, Dokki 12618, Giza, Egypt | ||
| 2Parasitology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt | ||
| 3Graduated from Faculty of Medicine, Mansoura University, 35516 Mansoura, Egypt | ||
| 4Department of Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam 70060, Pakistan | ||
| 5Ph.D. Student in Infectious Diseases, Transboundary Animal Diseases Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 8900065, Japan | ||
| 6Parasitology Department, Faculty of Veterinary Medicine, Minia University, Minya, Egypt | ||
| چکیده | ||
| Background: Equine piroplasmosis represents one of the main and serious health problems affecting the equines industry globally, caused by a tick-borne protozoa called Theileria equi and Babesia caballi. Aims: This study aimed to identify and genotype T. equi within the equine population from Giza Governorate, Egypt, by comparing the obtained results using the available diagnostic methods. Methods: We collected 116 apparently healthy horses from the study area during the first half of 2019 to identify T. equi using real-time PCR (qPCR), targeting the 18S rRNA gene. The results were compared with those from microscopic examination of Giemsa-stained blood smears and conventional PCR. Genotyping of the obtained sequences was also conducted to explore the genetic diversity of the detected T. equi strains. Results: Through sequencing and phylogenetic analysis, our samples were grouped into clusters corresponding to genotype A and genotype E. Our results demonstrated that the qPCR had the highest sensitivity (100%) followed by conventional PCR (68%) while microscopic examination had the lowest sensitivity (38%). Furthermore, the negative predictive value (NPV) of qPCR was the highest (100%) compared with conventional PCR and microscopical examination (80.49% and 68.04%, respectively) which revealed that negative cases detected by qPCR were certainly correct compared with the other two diagnostic assays. Conclusion: It is highly recommended to incorporate PCR diagnostic assays alongside microscopic examination to evaluate the epidemiological status of equine piroplasmosis. Also, our study demonstrated that T. equi genotype A and genotype E are circulating among Egyptian horses. | ||
| کلیدواژهها | ||
| Equine piroplasmosis؛ Microscopic examination؛ PCR؛ qPCR؛ T. equi | ||
| مراجع | ||
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