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In vitro investigation of the cytotoxic effects of various honeybee venoms on Caco-2 and T98G cells | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 2 - شماره پیاپی 91، 2025، صفحه 137-144 اصل مقاله (314.1 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.50439.7442 | ||
| نویسندگان | ||
| S. Pehlivan* 1، 2؛ E. Yarsan1 | ||
| 1Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey | ||
| 2Department of Pharmacology, School of Medicine, Ankara Medipol University, 06570 Ankara, Turkey | ||
| چکیده | ||
| Background: Honeybee (Apis mellifera) venom contains several biologically active peptides, including melittin, apamin, enzymes, and non-peptide components. Due to its biologically active components, honeybee venom demonstrates antibacterial, antiviral, anti-inflammatory, and cytotoxic properties. Aims: This study aimed to investigate the cytotoxic effects of honeybee venoms collected from different provinces in Turkey on colorectal adenocarcinoma (Caco-2) and glioblastoma multiforme (T98G) cell lines. Methods: The apamin, phospholipase A2, and melittin contents of honeybee venoms were analyzed using a high-pressure liquid chromatography (HPLC) variable wavelength detector. The viability of cells after 24-hour exposure to honeybee venoms was determined using the 3-(4,5-dimethylthiazol-2-yl) (ΜTT), neutral red (NR), and dehydrogenase leakage (LDH) assays. Results: Content analysis showed that Mugla had the highest apamin content, while Denizli had the highest phospholipase A2 and melittin contents among the analyzed bee venoms. For Caco-2 cells, the lowest inhibitory concentration (IC50) values were observed in the Denizli venom group, with MTT and LDH results of 12.42 ± 0.19 µg/ml and 8.19 ± 0.61 µg/ml, respectively. For T98G cells, these values were 5.98 ± 0.40 µg/ml and 5.04 ± 0.17 µg/ml, respectively. Conclusion: These findings indicate that honeybee venoms from different provinces contain varying levels of apamin, phospholipase A2, and melittin. The cytotoxic effects observed on Caco-2 and T98G cell lines suggest that honeybee venom may have potential as an anticancer agent. | ||
| کلیدواژهها | ||
| Apis mellifera؛ Cell culture؛ Cytotoxicity؛ HPLC؛ MTT | ||
| مراجع | ||
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