| تعداد نشریات | 24 |
| تعداد شمارهها | 867 |
| تعداد مقالات | 7,776 |
| تعداد مشاهده مقاله | 13,950,614 |
| تعداد دریافت فایل اصل مقاله | 11,954,026 |
In vitro investigation of the cytotoxic effects of various honeybee venoms on Caco-2 and T98G cells | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 2 - شماره پیاپی 91، 2025، صفحه 137-144 اصل مقاله (314.1 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.50439.7442 | ||
| نویسندگان | ||
| S. Pehlivan* 1، 2؛ E. Yarsan1 | ||
| 1Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey | ||
| 2Department of Pharmacology, School of Medicine, Ankara Medipol University, 06570 Ankara, Turkey | ||
| چکیده | ||
| Background: Honeybee (Apis mellifera) venom contains several biologically active peptides, including melittin, apamin, enzymes, and non-peptide components. Due to its biologically active components, honeybee venom demonstrates antibacterial, antiviral, anti-inflammatory, and cytotoxic properties. Aims: This study aimed to investigate the cytotoxic effects of honeybee venoms collected from different provinces in Turkey on colorectal adenocarcinoma (Caco-2) and glioblastoma multiforme (T98G) cell lines. Methods: The apamin, phospholipase A2, and melittin contents of honeybee venoms were analyzed using a high-pressure liquid chromatography (HPLC) variable wavelength detector. The viability of cells after 24-hour exposure to honeybee venoms was determined using the 3-(4,5-dimethylthiazol-2-yl) (ΜTT), neutral red (NR), and dehydrogenase leakage (LDH) assays. Results: Content analysis showed that Mugla had the highest apamin content, while Denizli had the highest phospholipase A2 and melittin contents among the analyzed bee venoms. For Caco-2 cells, the lowest inhibitory concentration (IC50) values were observed in the Denizli venom group, with MTT and LDH results of 12.42 ± 0.19 µg/ml and 8.19 ± 0.61 µg/ml, respectively. For T98G cells, these values were 5.98 ± 0.40 µg/ml and 5.04 ± 0.17 µg/ml, respectively. Conclusion: These findings indicate that honeybee venoms from different provinces contain varying levels of apamin, phospholipase A2, and melittin. The cytotoxic effects observed on Caco-2 and T98G cell lines suggest that honeybee venom may have potential as an anticancer agent. | ||
| کلیدواژهها | ||
| Apis mellifera؛ Cell culture؛ Cytotoxicity؛ HPLC؛ MTT | ||
| مراجع | ||
|
Altıntas, L and Bektas, N (2019). Apitherapy: 1. Bee venom. Uludag Bee J., 19: 82-95.
Arslan, P; Yurdakok Dikmen, B; Ozeren, SC and Kuzukıran, O (2021). In vitro effects of erythromycin and florfenicol on primary cell lines of Unio crassus and Cyprinus carpio. ESPR., 28: 48408-48416.
Aygun, G and Akbulak, C (2017). Evaluation of the organic livestock potential of Ardahan Province. Dumlupinar Univ. J. Soc. Sci., 53: 144-161.
Badria, F; Fathy, H; Fatehe, A; Elimam, D and Ghazy, M (2017). Evaluate the cytotoxic activity of honey, propolis, and bee venom from different localities in Egypt against liver, breast, and colorectal cancer. J. Apitherapy., 2: 1-4.
Baek, YH; Huh, JE; Lee, JD and Park, DS (2006). Antinociceptive effect and the mechanism of bee venom acupuncture (Apipuncture) on inflammatory pain in the rat model of collagen-induced arthritis: mediation by Α2-Adrenoceptors. Brain Res., 1073: 305-310.
Bazi, A; Gholamin, M; Sisakht, M and Keramati, MR (2015). Bee venom induces unfolded protein response in A172 glioblastoma cell line. Biotec. Health Sci., 2: e27547.
Bovi, TS; Onarı, P; Santos, SAA; Justulin, LA and Orsi, RO (2017). Apitoxin harvest impairs hypopharyngeal gland structure in Apis mellifera honey bees. Apidologie, 48: 755-760.
Büyüköztürk, Ş (2009). Data analysis handbook for social sciences. 10th Edn., Ankara, Turkey, Pegem Akademi Publishing. (in Turkish)
Cakmak, I; Fuchs, S; Cakmak, SS; Koca, AO; Nentchev, P and Kandemir, I (2014). Morphometric analysis of honeybees distributed in Northern Turkey along the Black Sea coast. Uludag Bee J., 14: 59-68.
Caprazlı, T and Kekecoglu, M (2021). Factors affecting the composition and production amount of honey bee venom. Uludag Bee J., 21: 132-145.
El-Wahed, AAA; Khalifa, SAM; Sheikh, BY; Farag, MA; Saeed, KA; Larik, FA; Koca Calıskan, U; Alajmi, MF; Hassan, M; Wahabi, HA; Hegazy, MEF; Algethami, AF; Buttner, S and Hesham, R (2019). Bee venom composition: from chemistry to biological activity. Stud. Nat. Prod. Chem., 60: 459-484.
Fabiani, R (2020). Antitumoral properties of natural products. Molecules, 25: 650.
Frangieh, J; Salma, Y; Haddad, K; Mattei, C; Legros, C; Fajloun, Z and El Obeid, D (2019). First characterization of the venom from Apis mellifera syriaca, a honeybee from the Middle East region. Toxins, 11: 191.
Gajski, G; ČimboraZovko, T; Rak, S; Rožman, M; Osmak, M and Garaj Vrhovac, V (2014). Combined antitumor effects of bee venom and cisplatin on human cervical and laryngeal carcinoma cells and their drug resistant sublines. J. Appl. Toxicol., 34: 1332-1341.
Gajski, G; Domijan, AM; Žegura, B; Štern, A; Gerić, M; Novak Jovanović, I; Vrhovac, I; Madunić, J; Breljak, D; Filipič, M and Garaj-Vrhovac, V (2016). Melittin induced cytogenetic damage, oxidative stress and changes in gene expression in human peripheral blood lymphocytes. Toxicon, 110: 56-67.
Gajski, G and Garaj-Vrhovac, V (2009). Radioprotective effects of honeybee venom (Apis mellifera) against 915-mhz microwave radiation-induced DNA damage in wistar rat lymphocytes: In vitro study. Int. J. toxicol., 28: 88-98.
Gajski, G and Garaj-Vrhovac, V (2011). Bee venom induced cytogenetic damage and decreased cell viability in human white blood cells after treatment in vitro: a multi-biomarker approach. Environ. Toxicol. Pharmacol., 32: 201-211.
Garaj-Vrhovac, V and Gajski, G (2009). Evaluation of the cytogenetic status of human lymphocytes after exposure to a high concentration of bee venom in vitro. Arh. Hig. Rada. Toksikol., 60: 27-34.
Haggar, FA and Boushey, RP (2009). Colorectal cancer epidemiology: Incidence, mortality, survival, and risk factors. Clin. Colon Rectal Surg., 22: 191-197.
Huang, M; Lu, JJ and Ding, J (2021). Natural products in cancer therapy: past, present and future. Nat. Prod. Bioprospect., 11: 5-13.
Ip, SW; Wei, HC; Lin, JP; Kuo, HM; Liu, KC; Hsu, SC; Yang, JS; Dueyang, M; Chiu, TH; Han, SM and Chung, JG (2008). Bee venom induced cell cycle arrest and apoptosis in human cervical epidermoid carcinoma Ca Ski cells. Anticancer Res., 28: 833-842.
Kence, A (2006). Genetic diversity of honey bees in Turkey and the importance of its conservation. Uludag Bee J., 6: 25-32.
Lebel, AA; Kisembo, MV; Soucy, MFN; Hébert, MP and Boudreau, LH (2021). Molecular characterization of the anticancer properties associated with bee venom and its components in glioblastoma multiforme. Chem. Biol. Interact., 347: 109622.
Lee, G and Bae, H (2016). Anti-inflammatory applications of melittin, a major component of bee venom: detailed mechanism of action and adverse effects. Molecules, 21: 616.
Lee, HL; Park, MH; Son, DJ; Song, HS; Kim, JH; Ko, SC; Song, MJ; Lee, WH; Yoon, JH; Young, WH; Han, SB and Hong, JT (2015). Anti-cancer effect of snake venom toxin through down regulation of AP-1 mediated PRDX6 expression. Oncotarget, 6: 22139-22151.
Moon, DO; Park, SY; Heo, MS; Kim, KC; Park, C; Ko, WS; Choi, YH and Kim, GY (2006). Key regulators in bee venom-induced apoptosis are Bcl-2 and caspase-3 in human leukemic U937 cells through downregulation of ERK and Akt. Int. Immunopharmacol., 6: 1796-1807.
Mosmann, T (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. JIM., 65: 55-63.
Oršolić, N (2012). Bee venom in cancer therapy. Cancer Metastasis Rev., 31: 173-194.
Oršolić, N; Šver, L; Verstovšek, S; Terzić, S and Bašić, I (2003). Inhibition of mammary carcinoma cell proliferation in vitro and tumor growth in vivo by bee venom. Toxicon, 41: 861-870.
Ozdemir, G; Ersoz, E and Dilek, NM (2021). Apitherapy and health. BSJ Health Sci., 4: 168-174.
Park, MH; Choi, MS; Kwak, DH; Oh, KW; Yoon, DY; Han, SB; Song, HS; Song, MJ and Hong, JT (2001). Anti-Cancer effect of bee venom in prostate cancer cells through activation of caspase pathway via inactivation of Nf-Κb. Prostate, 71: 801-812.
Ruttner, F; Pourasghar, D and Kauhausen, D (1985). The honeybees of Iran. 2. Apis mellifera meda Skorikow, the Persian honey bee. Apidologie. 16: 241-264. (in German) Rybak-Chmielewska, H and Szczesna, T (2004). HPLC study of chemical composition of honeybee (Apis mellifera L.) venom. JAS., 48: 103-109. Samancı, T and Kekecoglu, M (2019). Comparison of commercial and anatolian bee venom in terms of chemical composition. Uludag Bee J., 19: 61-68.
Senel, E and Demir, E (2018). Bibliometric analysis of apitherapy in complementary medicine literature between 1980 and 2016. Complement. Ther. Clin. Pract., 31: 47-52.
Sevin, S; Kıvrak, I; Tutun, H; Uyar, R and Ayaz, F (2022). Apis mellifera anatoliaca venom exerted anti-inflammatory activity on LPS-stimulated mammalian macrophages by reducing the production of the inflammatory cytokines. Appl. Biochem. Biotechnol., 195: 3194-3205.
Sıralı, R (2017). Some important characteristics of Anatolian bee (Apis mellifera anatoliaca). Uludag Bee J., 17: 82-92.
Sjakste, N and Gajski, G (2023). A review on genotoxic and genoprotective effects of biologically active compounds of animal origin. Toxins, 15: 165.
Smith, DR; Slaymaker, A; Palmer, M and Kaftanoglu, O (1997). Turkish honey bees belong to the east Mediterranean mitochondrial lineage. Apidologie, 28: 269-274.
Sobral, F; Sampaıo, A; Falcão, S; Queıroz, MJR; Calhella, RC; Vılas-Boas, M and Ferreıra, IC (2016). Chemical characterization, antioxidant, anti-inflammatory and cytotoxic properties of bee venom collected in northeast portugal. FCT., 94: 172-177.
Son, DJ; Lee, JW; Lee, YH; Song, HS; Lee, CK and Hong, JT (2007). Therapeutic application of anti-arthritis, pain-releasing, and anti-cancer effects of bee venom and its constituent compounds. Pharmacol. ther., 115: 246-270.
Tanugur Samanc, AE and Kekecoglu, M (2021). An evaluation of the chemical content and microbiological contamination of Anatolian bee venom. PloS One, 16: e0255161.
Zheng, J; Lee, HL; Ham, YW; Song, HS; Song, MJ and Hong, JT (2015). Anti-cancer effect of bee venom on colon cancer cell growth by activation of death receptors and inhibition of nuclear factor kappa B. Oncotarget, 6: 44437-44451. | ||
|
آمار تعداد مشاهده مقاله: 7 تعداد دریافت فایل اصل مقاله: 9 |
||