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Wharton's jelly mesenchymal stem cell-derived conditioned media inhibits colon cancer cells via activating AMPK/mTOR-mediated autophagy | ||
| Molecular Biology Research Communications | ||
| دوره 15، شماره 1، 2026، صفحه 31-38 اصل مقاله (498.82 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2025.52891.2133 | ||
| نویسندگان | ||
| Dian Dayer1؛ Zahra Akbari-Jonoush1، 2؛ Roya Mahdavi2، 3؛ Afshin Amari1، 2؛ Amirhesam Keshavarz-Zarjani1، 4؛ Layasadat Khorsandi* 1، 4 | ||
| 1Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran | ||
| 2Department of Immunology, School of Medicine Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran | ||
| 3Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran | ||
| 4Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran | ||
| چکیده | ||
| Prior studies have shown that conditioned media derived from Wharton's jelly mesenchymal stem cells (WJ-CM) have anti-cancer properties. This research investigated the impact of WJ-CM on HT-29 colorectal adenocarcinoma cells by examining autophagy biomarkers and the AMPK/mTOR pathway. The HT-29 cells were subjected to WJ-CM and an AMPK activator (AICAR). Autophagy and levels of AMPK and mTOR proteins were investigated. WJ-CM increased the expression of phosphorylated AMPK while reducing the level of phosphorylated mTOR in HT-29 cells. WJ-CM treatment elevated the LC3B/LC3A ratio and ATG7, ATG5, and Beclin-1 expression. However, there was a parallel drop in p62 expression, which indicates autophagy induction. AICAR increased the influence of WJ-CM on viability, as well as the levels of biomarkers associated with autophagy, phosphorylated AMPK, and phosphorylated mTOR in the HT-29 cells. WJ-CM inhibits colorectal cancer cell growth via activating AMPK/mTOR-mediated autophagy. | ||
| کلیدواژهها | ||
| Colon Cancer؛ AMPK؛ Stem Cell؛ Autophagy | ||
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