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Tao, Duhan, Takeuchi, Yoshinori, Karkoutly, Samia, Mehrazad Saber, Zahra, Chen, Ye, Mendsaikhan, Tsolmon, Saikawa, Rika, Aita, Yuichi, Murayama, Yuki, Shikama, Akito, Masuda, Yukari, Yahagi, Naoya. (1404). Analysis of amino acid residues affecting the transcriptional activity of nuclear factor Ya. , 15(1), 21-29. doi: 10.22099/mbrc.2025.54185.2206
Duhan Tao; Yoshinori Takeuchi; Samia Karkoutly; Zahra Mehrazad Saber; Ye Chen; Tsolmon Mendsaikhan; Rika Saikawa; Yuichi Aita; Yuki Murayama; Akito Shikama; Yukari Masuda; Naoya Yahagi. "Analysis of amino acid residues affecting the transcriptional activity of nuclear factor Ya". , 15, 1, 1404, 21-29. doi: 10.22099/mbrc.2025.54185.2206
Tao, Duhan, Takeuchi, Yoshinori, Karkoutly, Samia, Mehrazad Saber, Zahra, Chen, Ye, Mendsaikhan, Tsolmon, Saikawa, Rika, Aita, Yuichi, Murayama, Yuki, Shikama, Akito, Masuda, Yukari, Yahagi, Naoya. (1404). 'Analysis of amino acid residues affecting the transcriptional activity of nuclear factor Ya', , 15(1), pp. 21-29. doi: 10.22099/mbrc.2025.54185.2206
Tao, Duhan, Takeuchi, Yoshinori, Karkoutly, Samia, Mehrazad Saber, Zahra, Chen, Ye, Mendsaikhan, Tsolmon, Saikawa, Rika, Aita, Yuichi, Murayama, Yuki, Shikama, Akito, Masuda, Yukari, Yahagi, Naoya. Analysis of amino acid residues affecting the transcriptional activity of nuclear factor Ya. , 1404; 15(1): 21-29. doi: 10.22099/mbrc.2025.54185.2206

Analysis of amino acid residues affecting the transcriptional activity of nuclear factor Ya

Molecular Biology Research Communications
دوره 15، شماره 1، 2026، صفحه 21-29    اصل مقاله (499.1 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2025.54185.2206
نویسندگان
Duhan Tao1، 2؛ Yoshinori Takeuchi1؛ Samia Karkoutly1؛ Zahra Mehrazad Saber1؛ Ye Chen1، 2؛ Tsolmon Mendsaikhan1؛ Rika Saikawa1؛ Yuichi Aita1، 2؛ Yuki Murayama2؛ Akito Shikama2؛ Yukari Masuda1؛ Naoya Yahagi* 1، 2
1Division of Endocrinology and Metabolism, Department of Medicine, Jichi Medical University, Tochigi 329-0498, Japan
2Nutrigenomics Research Group, Institute of Medicine, University of Tsukuba, Ibaraki 305-8575, Japan
چکیده
Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor essential for regulating genes involved in lipid metabolism, including fatty acid synthase (Fasn). Although NF-Y activity is known to be dynamically regulated during adipocyte differentiation, the amino acid residues responsible for its transcriptional function remain unclear. In this study, we examined the contribution of nuclear factor Ya (NF-Ya) to DNA-binding activity and transcriptional regulation in 3T3-L1 adipocytes. Using deletion constructs and adenoviral expression systems, we identified a region spanning amino acid residues 184-347 of NF-Ya as critical for acquiring DNA-binding ability during differentiation. Electrophoretic mobility shift assays revealed that NF-Ya undergoes modification within this region, conferring stage-specific DNA-binding activity to the Fasn promoter. These findings suggest that post-translational modification of NF-Ya is a key mechanism regulating NF-Y function in adipogenesis. Our work provides novel insights into transcriptional control of lipogenic genes and their relevance to metabolic regulation.
کلیدواژه‌ها
Adipogenesis؛ Fatty acid synthesis؛ Transcription
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