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Rapid leukocyte esterase detection of cytological endometritis in dairy cows | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 1 - شماره پیاپی 90، 2025، صفحه 78-82 اصل مقاله (385.11 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.50736.7507 | ||
| نویسندگان | ||
| A. Sajadi1؛ M. Kafi* 2؛ H. Sharifiyazdi* 2؛ M. Azari2 | ||
| 1Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 2Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| Background: Certain limitations are associated with all kinds of diagnostic techniques used for the identification of bovine cytological endometritis under field conditions. Leukocyte esterase (LE) tape of urinary test strips has been used to detect cytological endometritis with an acceptable level of agreement with endometrial cytology in lactating dairy cows; however, the sampling procedure of LE tape strips is still time-consuming and not advisable for use at the farm level. Aims: The present study was designed to provide a simpler and faster LE test to improve the field applicability of LE tape strips for the detection of bovine cytological endometritis. Methods: Endometrial samples were collected using cytobrush and modified LE tape techniques from slaughtered (n=74) and live dairy cows (n=43). The urinary strip’s LE tape was affixed onto the cytobrush rod head using a hot glue adhesive. This modification created the possibility of rapid reading of the intensity of color change of LE tapes. Results: Considering the cytology as the reference method, the sensitivity and specificity of the LE method for slaughterhouse samples were 82% and 73%, respectively. The agreement between endometrial cytology and the modified LE technique, considering the combination of slaughterhouse and clinical samples, was found to be moderate (k=0.51; P<0.0001) in the present study. Conclusion: A rapid leukocyte esterase test can be used as a cow side and potential alternative to endometrial cytology; however, the efficacy of this method should further be evaluated based on the conception rates in dairy cows. | ||
| کلیدواژهها | ||
| Cytobrush؛ Cytological endometritis؛ Dairy cows؛ Leukocyte esterase | ||
| مراجع | ||
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Arango-Sabogal, JC; Dubuc, J; Krug, C; Denis-Robichaud, J and Dufour, S (2019). Accuracy of leukocyte esterase test, endometrial cytology and vaginal discharge score for diagnosing postpartum reproductive tract health status in dairy cows at the moment of sampling, using a latent class model fit within a Bayesian framework. Prev. Vet. Med., 162: 1-10.
Asfar, A; Sofi, KA; Bhat, MA; Malik, AA and Malik, AA (2021). Leukocyte esterase reagent strips: rapid, economical and reliable cow side test forsubclinical endometritis. Turk. J. Vet. Anim. Sci., 45: 540-546.
Barlund, CS; Carruthers, TD; Waldner, CL and Palmer, CW (2008). A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology. 69: 714-723.
Bogado Pascottini, OA (2016). Subclinical endometritis in dairy cattle: a practical approach. Ph.D. Thesis, School of Veterinary Medicine, Ghent University, Merelbeke, Belgium PP: 47-61.
Bruinjé, TC; Morrison, EI; Ribeiro, ES; Renaud, DL and LeBlanc, SJ (2024). Associations of inflammatory and reproductive tract disorders postpartum with pregnancy and early pregnancy loss in dairy cows. J. Dairy Sci., 107: 1630-1644.
Cheong, SH; Nydam, DV; Galvão, KN; Crosier, BM and Gilbert, RO (2011). Cow-level and herd-level risk factors for subclinical endometritis in lactating Holstein cows. J. Dairy Sci., 94: 762-770.
Cheong, SH; Nydam, DV; Galvão, KV; Crosier, BM; Ricci, A; Caixeta, LS; Sper, RB; Fraga, M and Gilbert, RO (2012). Use of reagent test strips for diagnosis of endometritis in dairy cows. Theriogenology. 77: 858-864.
Couto, GB; Vaillancourt, DH and Lefebvre, RC (2013). Comparison of a leukocyte esterase test with endometrial cytology for diagnosis of subclinical endometritis in postpartum dairy cows. Theriogenology. 79: 103-107.
Denis-Robichaud, J and Dubuc, J (2013). Validation of two diagnostic methods for postpartum endometritis in dairy cows. American Association of Bovine Practitioners Conference Proceedings. P: 135.
Denis-Robichaud, J and Dubuc, J (2015). Determination of optimal diagnostic criteria for purulent vaginal discharge and cytological endometritis in dairy cows. J. Dairy Sci., 98: 6848-6855.
Diaz-Lundahl, S; Heringstad, B; Garmo, RT; Gillund, P and Krogenæs, AK (2022). Heritability of subclinical endometritis in Norwegian Red cows. J. Dairy Sci., 105: 5946-5953.
Hart, DA (2015). Curbing inflammation in multiple sclerosis and endometriosis: Should mast cells be targeted? Int. J. Inflam., 2015: 452095.
Horlock, AD; Piersanti, RL; Ramirez-Hernandez, R; Yu, F; Ma, Z; Jeong, KC; Clift, MJD; Block, J; Santos, JEP; Bromfield, JJ and Sheldon, IM (2020). Uterine infection alters the transcriptome of the bovine reproductive tract three months later. Reproduction. 160: 93-107.
Kutter, D; Figueiredo, G and Klemmer, L (1987). Chemical detection of leukocytes in urine by means of a new multiple test strip. J. Clin. Chem. Clin. Biochem., 25: 91-94.
Leclere, M; Desnoyers, M; Beauchamp, G and Lavoie, JP (2006). Comparison of four staining methods for detection of mast cells in equine bronchoalveolar lavage fluid. J. Vet. Intern. Med., 20: 377-381.
Metz, M; Grimbaldeston, MA; Nakae, S; Piliponsky, AM; Tsai, M and Galli, SJ (2007). Mast cells in the promotion and limitation of chronic inflammation. Immunol. Rev., 217: 304-328.
Monument, MJ; Hart, DA; Salo, PT; Befus, AD and Hildebrand, KA (2013). Posttraumatic elbow contractures: targeting neuroinflammatory fibrogenic mechanisms. J. Orthop. Sci., 18: 869-877.
Noakes, DE (2019). Physiology of the puerperium. In: Noakes, DE; Parkinson, TJ and England,GCWEds.), Veterinary reproduction and obstetrics. (10th Edn.), Amsterdam, Elsevier. PP: 148-156.
Paiano, RB; Bonilla, J; Pugliesi, G; Moreno, AM and Baruselli, PS (2023). Evaluation of clinical and subclinical endometritis impacts on the reproductive performance and milk production of dairy cows in Brazilian herds. Reprod. Domest. Anim., 58: 414-422.
Paiano, RB; Moreno, LZ; Gomes, VT; Parra, BM; Barbosa, MR; Sato, MI; Bonilla, J; Pugliesi, G; Baruselli, PS and Moreno, AM (2022). Assessment of the main pathogens associated with clinical and subclinical endometritis in cows by culture and MALDI-TOF mass spectrometry identification. J. Dairy Sci., 105: 3367-3376.
Pinedo, P; Santos, JE; Chebel, RC; Galvão, KN; Schuenemann, GM; Bicalho, RC; Gilbert, RO; Zas, SR; Seabury, CM; Rosa, G and Thatcher, WW (2020). Early-lactation diseases and fertility in 2 seasons of calving across US dairy herds. J. Dairy Sci., 103: 10560-10576.
Ribeiro, ES; Gomes, G; Greco, LF; Cerri, RLA; Vieira-Neto, A; Monteiro, PLJ; Lima, FS; Bisinotto, RS; Thatcher, WW and Santos, JEP (2016). Carryover effect of postpartum inflammatory diseases on developmental biology and fertility in lactating dairy cows. J. Dairy Sci., 99: 2201-2220.
Ribeiro, ES; Lima, FS; Greco, LF; Bisinotto, RS; Monteiro, AP; Favoreto, M; Ayres, H; Marsola, RS; Martinez, N; Thatcher, WW and Santos, JE (2013). Prevalence of periparturient diseases and effects on fertility of seasonally calving grazing dairy cows supplemented with concentrates. J. Dairy Sci., 96: 5682-5697.
Sabattini, S; Renzi, A; Marconato, L; Militerno, G; Agnoli, C; Barbiero, L; Rigillo, A; Capitani, O; Tinto, D and Bettini, G (2018). Comparison between May-Grünwald-Giemsa and rapid cytological stains in fine-needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications. Vet. Comp. Oncol., 16: 511-517.
Santos, NR (2006). The use of leukocyte esterase reagent strips for diagnosis of subclinical endometritis in dairy cows. Theriogenology. 66: 666-667.
Sheldon, IM (2019). The metritis complex in cattle. In: Noakes, DE; Parkinson, TJ and England,GCW (Eds.), Veterinary reproduction and obstetrics. (10th Edn.), Amsterdam, Elsevier. PP: 408-433.
Van Schyndel, SJ; Pascottini, OB and LeBlanc, SJ (2018). Comparison of cow-side diagnostic techniques for subclinical endometritis in dairy cows. Theriogenology. 120: 117-122.
Wagener, K; Gabler, C and Drillich, M (2017). A review of the ongoing discussion about definition, diagnosis and pathomechanism of subclinical endometritis in dairy cows. Theriogenology. 94: 21-30. | ||
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