| تعداد نشریات | 24 |
| تعداد شمارهها | 912 |
| تعداد مقالات | 8,158 |
| تعداد مشاهده مقاله | 15,837,021 |
| تعداد دریافت فایل اصل مقاله | 13,332,469 |
Molecular typing and seroepidemiology of pathogenic Leptospira spp. of domestic animals in Gilan province, northern Iran during 2021-2023 | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 1 - شماره پیاپی 90، 2025، صفحه 41-50 اصل مقاله (1.3 M) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.48812.7141 | ||
| نویسندگان | ||
| B. Talebi Darestani1؛ S. T. Shafighi* 2؛ G. Abdollahpour3؛ L. Asadpour2؛ N. Ranji2 | ||
| 1Ph.D. Student in Microbiology, Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| 2Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| 3Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Background: Leptospira, a pathogenic bacterial organism, causes human leptospirosis when it infects mammals. There are different serovars of Leptospira, each of which is adapted to a specific animal host. Adaptation occurs dynamically in different hosts and changes the distribution and clinical manifestations of serovars. Variable-number tandem repeats (VNTRs) have been used to identify Leptospira interrogans strains. Aims: The purpose of this study was to conduct seroepidemiology and molecular typing of pathogenic Leptospira isolated from domestic animals in Gilan province. Methods: A total number of 250 urine and blood samples were collected from domestic animals (horse, bovine, and sheep). Urine samples were cultured in EMJH medium. Using the microscopic agglutination test (MAT) and the Multilocus VNTR Analysis (MLVA) methods, unknown samples were analyzed to determine Leptospira serovars. Results: Based on our data, Leptospira Autumnalis was mainly found in horses and dominated at 50%, followed by Leptospira Canicola at 29.16%. MAT results showed that among 87 animals with the seropositive, the horses showed the highest level of contamination at 40%, and this difference was not significant (P=0.152). Conclusion: According to our data, Leptospira Autumnalis was mainly found in horses, and the bacteria were shed in urine, suggesting that horses could transmit leptospirosis. The results of this study confirm that MLVA can replace time-consuming methods such as MAT because it is a simple and fast technique. | ||
| کلیدواژهها | ||
| Leptospira؛ Microscopic agglutination test؛ Molecular typing؛ Multilocus VNTR analysis | ||
| مراجع | ||
|
Abdollahpour, G; Shafighi, T and Sattari Tabrizi, S (2009). Serodiagnosis of leptospirosis in cattle in north of Iran, Gilan. Int. J. Vet. Res., 3: 7-10.
Beer, DH (2016). The biological standard of living in Suriname, c. 1870-1975. Econ. Hum. Biol., 22: 140-154.
Borumand Azad, F; Tadayon, K; Nofeli, M; Qaderi, R; Sekhavati, M; Saeedi, S; Fattah Moghaddam, M and Abbasi, E (2017). Introducing two new loci of VNTR9 and VNTR10 for Bordetella pertussis genotyping. Urmia Med. J., 28: 8.
Brooks, G; Carroll, CK; Butel, J and Morse, S (2012). Jawetz melnick and adelbergs medical microbiology. 26th Edn., Baltimore, Maryland. McGraw-Hill Education Publishing. PP: 504-507. https://public.ebookcentral. proquest.com/choice.
Caimi, K; Varni, V; Melendez, Y; Koval, A; Brihuega, B and Ruybal, P (2012). A combined approach of VNTR and MLST analysis: improving molecular typing of Argentinean isolates of Leptospira interrogans. Mem. Inst. Oswaldo. Cruz., 107: 644-651. doi: 10.1590/s0074-02762012000500011.
Calì, F; Dianzani, I; Desviat, LR; Perez, B; Ugarte, M; Ozguc, M; Seyrantepe, V; Shiloh, Y; Giannattasio, S and Carducci, C (1997). The STR252-IVS10nt546-VNTR7 phenylalanine hydroxylase minihaplotype in five Mediterranean samples. Hum. Genet., 100: 350-355.
Cilia, G; Bertelloni, F; Piredda, I; Nicoletta Ponti, M; Turchi, B; Cantile, C; Parisi, F; Pinzauti, P; Armani, A and Palmas, B (2020). Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy. PLoS Negl. Trop. Dis., 14: 1-12. doi: 10.1371/journal.pntd.e0008982.
Dalir-Naghadeh, B; Seifi, HA; Asri-Rezaei, S and Pilevary, N (2006). Post-parturient haemoglobinuria in Iranian river buffaloes: a preliminary study. Comp. Clin. Path., 14: 221-225.
Fang, R; Li, X; Li, J; Wu, J; Shen, X; Gui, X; DeRiemer, K; Liu, L; Mei, J and Gao, Q (2008). Mixed infections of Mycobacterium tuberculosis in tuberculosis patients in Shanghai, China. Tuberculosis. 88: 469-473.
Fawzy, A; Zschöck, M; Ewers, C and Eisenberg, T (2016). New polymorphisms within the variable number tandem repeat (VNTR) 7 locus of Mycobacterium avium subsp. Paratuberculosis. Mol. Cell. Probes., 30: 132-137.
Gomes-Solecki, M; Santecchia, I and Werts, C (2017). Animal models of leptospirosis: of mice and hamsters. Front. Immunol., 8: 1-20. doi: 10.3389/fimmu.2017.00058.
Kakita, T; Kuba, Y; Kyan, H; Okano, S; Morita, M and Koizumi, N (2021). Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan. Sci. Rep., 11: 1-8.
Khaki, P (2013). Genotyping of Razi vaccinal Leptospira Serovars based on VNTR (MLVA) method. agris.fao.org., 108.
Koizumi, N; Izumiya, H and Ohnishi, M (2020). Genetic relatedness of Leptospira interrogans serogroup Autumnalis isolated from humans, dogs, and mice in Japan. BMC Res. Notes. 13: 369-372. https://doi.org/10.1186/ s13104-020-05211-1.
Loffler, GS; Samartino, LE and Birhuega, B (2017). Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes. J. Zoonotic Dis. Pub. Health. 1: 1-8.
Muz, SE; Özdaş, S; Özdaş, T; Atilla, MH; Baştimur, S; Öz, I and Canatar, İ (2021). Protective effect of 5-HTTLPR (S) and VNTR (10) allele combinations of 5-HTT gene against adenotonsillary hypertrophy. Kocaeli. Med. J., 10: 6-15.
Nadon, CA; Trees, E; Ng, LK; Nielsen, EM; Reimer, A; Maxwell, N; Kubota, KA; Gerner-Smidt, P and Collective the MLVA Harmonization Working Group (2013). Development and application of MLVA methods as a tool for inter-laboratory surveillance. Euro Surveill., 18: 20565-20588.
Nalam, K; Ahmed, A; Devi, SM; Francalacci, P; Baig, M; Sechi, LA; Hartskeerl, RA and Ahmed, N (2010). Genetic affinities within a large global collection of pathogenic Leptospira: implications for strain identification and molecular epidemiology. PLoS One. 5: e12637.
Piredda, I; Ponti, MN; Palmas, B; Noworol, M; Pedditzi, A; Rebechesu, L and Chisu, V (2021). Molecular typing of pathogenic Leptospira species isolated from wild mammal reservoirs in Sardinia. Animals. 11: 1109-1122.
Postollec, F; Falentin, H; Pavan, S; Combrisson, J and Sohier, D (2011). Recent advances in quantitative PCR (qPCR) applications in food microbiology. Food Microbiol., 28: 848-861.
Rajeev, S (2022). Spirochetes V: Leptospira. Vet. Microbiol., 1: 225-230. https://doi.org/10.1002/9781119650836.ch24.
Ramin, AG and Azizzadeh, F (2013). Seroepidemiological detection of antibodies against Leptospira spp. using microscopic agglutination test in Urmia cows and sheep. Acta Vet., 63: 53-61.
Ramírez-Patiño, R (2013). Intron 4 VNTR (4a/b) polymorphism of the endothelial nitric oxide synthase gene is associated with breast cancer in Mexican women. J. Korean. Med. Sci., 28: 1587-1594.
Rees, EM; Minter, A; Edmunds, WJ; Lau, CL; Kucharski, AJ and Lowe, R (2021). Transmission modelling of environmentally persistent zoonotic diseases: a systematic review. Lancet Planet. Health. 5: e466-e478.
Rezasoltani, S; Dabiri, H; Khaki, P; Rostami nejad, M; Karimnasab, N and Modirrousta, S (2015). Characterization of Leptospira interrogans serovars by polymorphism variable number tandem repeat analysis. Jundishapur J. Microbiol., 8: e22819-e22825.
Salaün, L; Merien, F; Gourianova, S; Baranton, G and Picardeau, M (2006). Application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of leptospirosis. J. Clin. Microbiol., 44: 3954-3962. doi: 10.1128/JCM.00336-06.
Saravanan, R; Rajendran, P; Thyagarjan, SP; Smythe, LD; Norris, MA; Symonds, ML and Dohnt, MF (2000). Leptospira autumnalis isolated from a human case from Avadi, India, and the serovar’s predominance in local rat and bandicoot populations. Ann. Trop. Med. Parasitol., 94: 503-506. doi: 10.1080/00034983.2000.11813569.
Sivakumar, RR (2022). Ocular leptospirosis: lack of awareness among ophthalmologists and challenges in diagnosis. Curr. Opin. Ophthalmol., 33: 532-542.
Strain, EMA; Lai, RWS; White, CA; Piarulli, S; Leung, KMY; Airoldi, L and O’Brien, A (2022). Editorial: Marine pollution-emerging issues and challenges. Front. Mar. Sci., 9: 918984-918993.
Sykes, JE; Reagan, KL; Nally, JE; Galloway, RL and Haake, DA (2022). Role of diagnostics in epidemiology, management, surveillance, and control of leptospirosis. Pathogens. 11: 395-419.
Tomazou, E; Rakyan, V; Lefebvre, G; Andrews, R; Ellis, P; Jackson, D; Langford, C; Francis, MD; Backdahl, L; Miretti, M; Coggill, P; Ottaviani, D; Sheer, D; Murrell, A and Beck, S (2008). Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis. BMC Med. Genomics. 1: 19-35.
Varni, V; Ruybal, P; Lauthier, JJ; Tomasini, N; Brihuega, B; Koval, A and Caimi, K (2014). Reassessment of MLST schemes for Leptospira spp. typing worldwide. Infect. Genet. Evol., 22: 216-222.
Vincent, AT; Schiettekatte, O; Goarant, C; Neela, VK; Bernet, E; Thibeaux, R; Ismail, N; Mohd Khalid, MKN; Amran, F and Masuzawa, T (2019). Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics. PLoS Negl. Trop. Dis., 13: e0007270-e0007295. doi: 10.1371/journal. pntd.0007270.
Viriyakosol, S; Matthias, MA; Swancutt, MA; Kirkland, TN and Vinetz, JM (2006). Toll-like receptor 4 protects against lethal Leptospira interrogans serovar icterohaemorrhagiae infection and contributes to in vivo control of leptospiral burden. Infect. Immun., 74: 887-895.
Xu, Y; Zhu, Y; Wang, Y; Chang, YF; Zhang, Y; Jiang, X; Zhuang, S; Zhu, Y; Zhang, J and Zeng, L (2016). Whole genome sequencing revealed host adaptation-focused genomic plasticity of pathogenic Leptospira. Sci. Rep., 6: 1-11. Yadeta, W; Bashahun, GM and Abdela, N (2016). Leptospirosis in animal and its public health implications: A review. World Appl. Sci. J., 34: 845-853.
Zamanmirabadi, A; Hemmati, R; Dolatabadian, A and Batley, J (2022). Genetic structure and phylogenetic relationships of Leptosphaeria maculans and L. biglobosa in Northern regions of Iran. Arch. Phytopathol. Plant Prot., 55: 1062-1081. | ||
|
آمار تعداد مشاهده مقاله: 374 تعداد دریافت فایل اصل مقاله: 246 |
||