| تعداد نشریات | 24 |
| تعداد شمارهها | 882 |
| تعداد مقالات | 7,867 |
| تعداد مشاهده مقاله | 14,715,658 |
| تعداد دریافت فایل اصل مقاله | 12,560,193 |
Molecular and phenotypic characteristics of isolated Escherichia coli from the skin, gills, and intestine of rainbow trout in retail stores of Kerman, Iran | ||
| Iranian Journal of Veterinary Research | ||
| دوره 26، شماره 1 - شماره پیاپی 90، 2025، صفحه 17-26 اصل مقاله (609.56 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2025.49736.7331 | ||
| نویسندگان | ||
| P. Mohseni1؛ R. Ghanbarpour* 2؛ M. Jajarmi2؛ M. Bagheri3 | ||
| 1Ph.D. in Bacteriology, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 3Department of Food Science and Technology, Bardsir Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| چکیده | ||
| Background: Escherichia coli is not naturally present in fish microbiota but can be acquired from sewage-contaminated aquatic environments. Aims: This study was performed to isolate and characterize the E. coli strains in rainbow trout fish marketed for human consumption. Methods: A total number of 166 fish were randomly collected from different retail settings in Kerman, Iran. The fish samples were analyzed to detect E. coli isolates. Antimicrobial resistance genes, Shiga toxin virulence subtypes and phylogenetic sequences were screened by PCR. Results: Prevalence of E. coli isolates on the skin, in the gills and intestine were 76.5% (127/166), 6.6% (11/166), and 3% (5/166), respectively. The most prevalent antimicrobial resistance phenotypes were observed against florfenicol (86.61%), erythromycin (83.46%), flumequine (82.67%), and oxytetracycline (81.88%); and 98.42% of the isolates were multi-drug resistant. The most frequent resistance gene was blaTEM (14.17%), followed by qnrA (10.23%), tetB (9.44%), sul2 (8.66%), blaSHV (7.87%), sul1 (7.87%), dhfr1 (3.93%), blaCTX-M (3.14%), dhfrV (1.57%), blaOXA (0.78%). Totally, 8.66% of isolates were categorized into three pathotypes including STEC, EPEC and EHEC. The stx subtypes including stx1a, stx1c, stx1d, stx2c, stx2d, stx2e and stx2f were identified in stx-positive strains. The E. coli isolates were classified into five phylogenetic groups including A (23.62%), B2 (3.93%), D (2.36%), F (9.44%) and cryptic clade I (11.81%). Conclusion: The study revealed that the skin of retail rainbow trout marketed in Kerman may be one of the potential passive carriers of multi-drug resistant and virulent E. coli strains. | ||
| کلیدواژهها | ||
| Escherichia coli؛ Drug resistance؛ Fish؛ Phylogenetic groups؛ Virulence | ||
| مراجع | ||
|
Addae-Nuku, DS; Kotey, FCN; Dayie, NTKD; Osei, MM; Tette, EMA; Debrah, P and Donkor, ES (2022). Multidrug-resistant bacteria in hospital wastewater of the Korle Bu teaching hospital in Accra, Ghana. Environ. Health Insights. 16: 1-. https://doi.org/10.1177/ 11786302221130613.
Ahmed, AM; Rashad, NR; Ibrahim, AIY; Abdel-Wahab, MM and Abdel-Wahab, MA (2023). Occurrence of phoA and shiga toxin genes in marketed Gandoffli, Ruditapes decussates. J. Adv. Vet. Res., 13: 469-473.
Al Qabili, DMA; Aboueisha, AKM; Ibrahim, GA; Youssef, AI and El-Mahallawy, HS (2022). Virulence and antimicrobial-resistance of shiga toxin-producing E. coli (STEC) isolated from edible shellfish and its public health significance. Arch. Microbiol., 204(510): 1-7.
Alttai, NA; Al-Sanjary, RA and Sheet, OH (2023). Isolation and molecular identification of Escherichia coli strain from fish available in farms and local markets in Nineveh governorate, Iraq. Iraqi J. Vet. Sci., 37: 431-435.
Andreoli, V; Bagliani, M; Corsi, A and Frontuto, V (2021). Drivers of protein consumption: a cross-country analysis. Sustainability. 13(7399): 1-19.
Austin, B (2006). The bacterial microflora of fish, revised. Sci. World J., 6: 931-945.
Brahmi, S; Touati, A; Dunyach-Remy, C; Sotto, A; Pantel, A and Lavigne, JP (2018). High prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae in wild fish from the Mediterranean sea in Algeria. Microb. Drug Resist., 24: 290-298.
Cardozo, MV; Borges, CA; Beraldo, LG; Maluta, RP; Pollo, AS; Borzi, MM; dos Santos, LF; Kariyawasam, S and Ávila, FA (2018). Shigatoxigenic and atypical enteropathogenic Escherichia coli in fish for human consumption. Braz. J. Microbiol., 49: 936-941.
Clermont, O; Christenson, JK; Denamur, E and Gordon, DM (2013). The clermont Escherichia coli phylo-typing method revisited: Improvement of specificity and detection of new phylo-groups. Environ. Microbiol. Rep., 5: 58-65.
CLSI (2018). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals (CLSI supplement VET08). 4th Edn., Wayne, PA, USA: Clinical and Laboratory Standards Institute. PP: 20-33.
Divya, PS; Thajudeen, J; Yousuf, J; Madavan, A and Abdulla, MH (2020). Genetic relatedness, phylogenetic groups, antibiotic resistance, and virulence genes associated with ExPEC in Escherichia coli isolates from finfish and shellfish. Food Process. Preserv., 44(e14837): 1-13.
Done, HY; Venkatesan, AK and Halden, RU (2015). Does the recent growth of aquaculture create antibiotic resistance threats different from those associated with land animal production in agriculture? AAPS J., 17: 513-524.
Dutta, C and Sengupta, C (2016). Prevalence of Escherichia coli in fish and shrimps obtained from retail fish markets in and around Kolkata, India. Front. Environ. Microbiol., 2: 1-5.
Ellis-Iversen, J; Seyfarth, AM; Korsgaard, H; Bortolaia, V; Munck, N and Dalsgaard, A (2020). Antimicrobial resistant E. coli and enterococci in pangasius fillets and prawns in Danish retail imported from Asia. Food Control. 114(106958): 1-6.
Fernandes, R; Abreu, R; Serrano, I; Such, R; Garcia-Vila, E; Quirós, S; Cunha, E; Tavares, L and Oliveira, M (2024). Resistant Escherichia coli isolated from wild mammals from two rescue and rehabilitation centers in Costa Rica: characterization and public health relevance. Sci. Rep., 14(8039): 1-14.
Hassen, B; Jouini, A; Elbour, M; Hamrouni, S and Maaroufi, A (2020). Detection of extended-spectrum β-lactamases (ESBL) producing Enterobacteriaceae from fish trapped in the lagoon area of Bizerte, Tunisia. Biomed. Res. Int., 2020(7132812): 1-9.
Hudzicki, J (2009). Kirby-Bauer disk diffusion susceptibility test protocol. ASM, 15: 55-63.
Ishida, Y; Ahmed, AM; Mahfouz, NB; Kimura, T; El-Khodery, SA; Moawad, AA and Shimamoto, T (2010). Molecular analysis of antimicrobial resistance in gram-negative bacteria isolated from fish farms in Egypt. J. Vet. Med. Sci., 72: 727-734.
Jagoda, S; Wijewardana, T; Arulkanthan, A; Igarashi, Y; Tan, E; Kinoshita, S; Watabe, S and Asakawa, S (2014). Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia. Dis. Aquat. Organ., 109: 127-137.
Kouadio-N’gbesso, N; Kouassi, N; N’guessan, FK; Adingra, A; Yobouet, BA; Dadié, A and Djè, KM (2016). Relationship between the phylogenetic group of Escherichia coli strains isolated in water and fish in fresco lagoon (Côte d’Ivoire). Int. J. Curr. Microbiol. Appl. Sci., 5(10): 413-423.
Kusunur, AB; Kuraganti, GK; Mogilipuri, SS; Vaiyapuri, M; Narayanan, SV and Badireddy, MR (2022). Multidrug resistance of Escherichia coli in fish supply chain: A preliminary investigation. J. Food Saf., 42(e12972): 1-13.
Liao, CY; Balasubramanian, B; Peng, JJ; Tao, SR; Liu, WC and Ma, Y (2021). Antimicrobial resistance of Escherichia coli from aquaculture farms and their environment in Zhanjiang, China. Front. Vet. Sci., 8(1534): 1-.
Lima, LS; Proietti-Junior, AA; Rodrigues, YC; da Silva Vieira, MC; Lima, LNGC; de Oliveira Souza, C; Dias Gonçalves, V; de Oliveira Lima, M; dos Prazeres Rodrigues, D and Lima, KVB (2022). High genetic diversity and antimicrobial resistance in Escherichia coli highlight Arapaima gigas (Pisces: Arapaimidae) as a reservoir of quinolone-resistant strains in Brazilian amazon rivers. Microorganisms. 10(808): 1-16.
Mohseni, P; Ghanbarpour, R; Jajarmi, M and Bagheri, M (2023). Antibiotic resistance phenotypes and genes of Escherichia coli isolates from rainbow trout (Oncorhynchus mykiss) sold in retail settings in Kerman, Iran. Iran. Vet. J., 19: 51-63.
Naderi, Z; Ghanbarpour, R and Sami, M (2016). Antimicrobial resistance characteristics and phylogenetic groups of Escherichia coli isolated from diarrheic calves in southeast of Iran. Int. J. Enteric Pathog., 4: 1-7.
Nadimpalli, M; Vuthy, Y; de Lauzanne, A; Fabre, L; Criscuolo, A; Gouali, M; Huynh, BT; Naas, T; Phe, T and Borand, L (2019). Meat and fish as sources of extended-spectrum β-Lactamase-producing Escherichia coli, Cambodia. Emerg. Infect. Dis., 25(1): 126-131.
Odumosu, BT; Obeten, HI and Bamidele, TA (2021). Incidence of multidrug-resistant Escherichia coli harbouring blaTEM and tetA genes isolated from seafoods in Lagos Nigeria. Curr. Microbiol., 78: 2414-2419.
Onmaz, NE; Yildirim, Y; Karadal, F; Hizlisoy, H; Al, S; Gungor, C; Disli, HB; Barel, M; Dishan, A and Tegin, RAA (2020). Escherichia coli O157 in fish: Prevalence, antimicrobial resistance, biofilm formation capacity, and molecular characterization. LWT, 133(109940): 1-12.
Paton, AW and Paton, JC (1998). Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays for stx 1, stx 2, eaeA, Enterohemorrhagic E. coli hlyA, rfb O111, and rfb O157. J. Clin. Microbiol., 36: 598-602.
Paton, AW and Paton, JC (2002). Direct detection and characterization of shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J. Clin. Microbiol., 40: 271-274.
Prisca Aleru, C (2022). Detection of resistance genes in Escherichia coli isolated from fishes and shellfishes in Creek Road/Bonny Estuary, Port Harcourt, Nigeria. Microbiol. Res. J. Int., 32: 11-22.
Ryu, SH; Park, SG; Choi, SM; Hwang, YO; Ham, HJ; Kim, SU; Lee, YK; Kim, MS; Park, GY; Kim, KS and Chae, YZ (2012). Antimicrobial resistance and resistance genes in Escherichia coli strains isolated from commercial fish and seafood. Int. J. Food Microbiol., 152: 14-18.
Saharan, VV; Verma, P and Singh, AP (2020). High prevalence of antimicrobial resistance in Escherichia coli, Salmonella spp. and Staphylococcus aureus isolated from fish samples in India. Aquac. Res., 51: 1200-1210.
Schar, D; Klein, EY; Laxminarayan, R; Gilbert, M and Van Boeckel, TP (2020). Global trends in antimicrobial use in aquaculture. Sci. Rep., 10(21878): 1-10.
Scheutz, F; Teel, LD; Beutin, L; Piérard, D; Buvens, G; Karch, H; Mellmann, A; Caprioli, A; Tozzoli, R and Morabito, S (2012). Multicenter evaluation of a sequence-based protocol for subtyping shiga toxins and standardizing Stx nomenclature. J. Clin. Microbiol., 50: 2951-2963.
Shah, SQA; Colquhoun, DJ; Nikuli, HL and Sørum, H (2012). Prevalence of antibiotic resistance genes in the bacterial flora of integrated fish farming environments of Pakistan and Tanzania. Environ. Sci. Technol., 46: 8672-8679. Sheng, L and Wang, L (2021). The microbial safety of fish and fish products: Recent advances in understanding its significance, contamination sources, and control strategies. Compr. Rev. Food Sci. Food Saf., 20: 738-786.
Some, S; Mondal, R; Mitra, D; Jain, D; Verma, D and Das, S (2021). Microbial pollution of water with special reference to coliform bacteria and their nexus with environment. Energy Nexus. 1(100008): 1-9.
Tilahun, A and Engdawork, A (2020). Isolation, identification and antimicrobial susceptibility profile of E. coli (O157: H7) from fish in lake Hawassa, southern Ethiopia. Life Sci. J., 17: 64-72.
Tran, HL; Hong, MH and Tran, TTH (2018). Antibiotic resistance and molecular characteristics of extended-spectrum beta-lactamase-producing Escherichia coli isolated from fish pond. CTU J. Sci., 54: 114-123.
Tumacácori, A (2019). Escherichia coli in the Santa Cruz River in Tumacácori National Historical Park, Arizona. 1st Edn., Tucson, AZ, USA: USGS Arizona Water Science Center. PP: 1-6.
WHO (2014). Report of the fifth meeting of the international coordinating group of the World Health Organization and the Bill & Melinda gates foundation project on eliminating human and dog rabies: Dar es Salaam, United Republic of Tanzania, 8-10 October 2013.
Yohans, H; Mitiku, BA and Tassew, H (2022). Levels of Escherichia coli as bio-indicator of contamination of fish food and antibiotic resistance pattern along the value chain in Northwest Ethiopia. Vet. Med. Res. Rep., 13: 299-311. | ||
|
آمار تعداد مشاهده مقاله: 370 تعداد دریافت فایل اصل مقاله: 278 |
||