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SPIN.DOC induces cellular transformation of NIH3T3 normal mouse fibroblast cells | ||
| Molecular Biology Research Communications | ||
| دوره 14، شماره 4، 2025، صفحه 283-289 اصل مقاله (405.77 K) | ||
| نوع مقاله: Short communication | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2025.52821.2125 | ||
| نویسندگان | ||
| Khuraijam Mrinalini Devi1؛ Thangjam Davis Singh1؛ Rubismita Deka2؛ Lisam Shanjukumar Singh1؛ Thiyam Ramsing Singh* 2 | ||
| 1Department of Biotechnology, Manipur University, Imphal -795003, India | ||
| 2Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784028, Assam, India | ||
| چکیده | ||
| SPIN.DOC was discovered as a transcriptional co-repressor of wingless related integration site (WNT) pathway. However, it has been found to be upregulated in various types of cancer, including hepatocellular carcinoma, colorectal cancer, renal papillary cell carcinoma. Whether SPIN.DOC functions as an oncogene or tumour suppressor gene remains uncertain. Here, we report that ectopic expression of SPIN.DOC in normal NIH3T3 fibroblast cells promotes cell proliferation, colony formation, migration and invasion. Moreover, SPIN.DOC expressing NIH3T3 cells show increased spheroid formation, suggesting enhanced stemness and transformation potential. Immunofluorescence analysis using anti-β-Tubulin suggests that SPIN.DOC may induce, formation of tubulin-based microtentacles (McTNs), indicating epithelial-to-mesenchymal (EMT) transition. In conclusion, our study helps in establishing that SPIN.DOC can function as an oncogene. | ||
| کلیدواژهها | ||
| SPIN.DOC؛ NIH3T3؛ Oncogene؛ Cancer stem cell؛ Microtentacle | ||
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