| تعداد نشریات | 24 |
| تعداد شمارهها | 874 |
| تعداد مقالات | 7,803 |
| تعداد مشاهده مقاله | 14,220,754 |
| تعداد دریافت فایل اصل مقاله | 12,165,055 |
Genetic and phylogenetic evaluations of Schistosoma turkestanicum isolated from goats in Western Iran | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 3 - شماره پیاپی 88، 2024، صفحه 273-278 اصل مقاله (1.43 M) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.49135.7203 | ||
| نویسندگان | ||
| H. Nayebzadeh* 1؛ N. Nazifi2؛ H. R. Shokrani1؛ Sh. Yavari3 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran | ||
| 2Department of Basic Science, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran | ||
| 3Ph.D. in Parasitology, Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran | ||
| چکیده | ||
| Background: Schistosomiasis, a zoonotic parasitic infection, poses significant challenges to the animal husbandry industry, leading to substantial economic losses. Despite its impact, there is limited data on the genotypes of Schistosoma (=Orientobilharzia) turkestanicum in Iran. Aims: The present study aimed to evaluate the phylogenetic relationships of Schistosoma turkestanicum isolated from goats by analyzing the mitochondrial cytochrome C oxidase subunit 1 (Cox1) gene sequence in Lorestan province, located in Western Iran. Methods: DNA extraction was performed on 20 male parasitic worms, and the mitochondrial Cox1 gene was amplified using the polymerase chain reaction (PCR) process, and sent for sequencing after purification by ethanol alcohol. The sequences were trimmed using CLC Main Workbench software. Phylogenetic analysis was conducted using the Neighbor-Joining method with 1000 bootstrap replicates in MEGA6 software to assess evolutionary relationships between the Cox1 gene sequence obtained in this study (GenBank accession No. PP627151) and various S. turkestanicum sequences obtained from the National Center for Biotechnology Information (NCBI). Additionally, this software was also used to plot the genetic distance matrix (nucleotide differences and similarities). Results: Phylogenetic analysis confirmed that the parasite isolated in this study was S. turkestanicum. Conclusion: The findings indicate that the S. turkestanicum lineage identified in this study is closely related to those found in Mazandaran province, Iran, as well as to African Schistosoma species. | ||
| کلیدواژهها | ||
| Goat؛ Iran؛ Phylogeny؛ Polymerase chain reaction؛ Schistosoma | ||
| مراجع | ||
|
Abdigoudarzi, M and Karimi, G (2020). Estimation of schistosomiosis infection rate in the sheep in Shiraz region, Fars Province, Iran. J. Istanbul Vet. Sci., 4: 26-30.
Aldhoun, J and Littlewood, D (2012). Orientobilharzia Dutt & Srivastava, 1955 (Trematoda: Schistosomatidae), a junior synonym of Schistosoma Weinland 1858. Syst. Parasitol., 82: 81-88.
Attwood, SW; Upatham, ES; Meng, XH; Qiu, DC and Southgate, VR (2002). The phylogeography of Asian Schistosoma (Trematoda: Schistosomatidae). Parasitology. 125: 99-112.
Bhalerao, GD (1932). On the identity of the schistosome found in cases of bovine nasal granuloma and some observations on a few other members of the Schistosomatidae. Ind. J. Vet. Sci. Anim. Husb., 2: 338-356.
Brant, SV and Loker, ES (2005). Can specialized pathogens colonize distantly related hosts? Schistosome evolution as a case study. PLoS Pathog., 1: e38.
Dutt, SC and Srivastava, HD (1952). On the morphology and life history of a new mammalian blood-fluke Orientobilharzia dattai n. sp. Parasitology. 42: 144-150.
Eslami, A (1998). Trematoda veterinary helminthology. 2nd Edn., Tehran, Tehran University Publications. PP: 148-175.
Folmer, O; Black, M; Hoeh, W; Lutz, R and Vrijenhoek, R (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3: 294-299.
Ghadirian, E and Hoghooghi, N (1973). The presence of snails of veterinary importance in Isfahan Iran. Bri. Vet. J., 129: 1-3.
Hebert, PD; Cywinska, A; Ball, SL and DeWaard, JR (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Proc. Biol. Sci. B, 270: 313-321.
Hosseini, SH; Eslami, A and Haddadzadeh, H (1997). Epidemiological approach to the ornithobilharziosis in sheep in Babolsar (Mazandaran). Iran J. Fac. Vet. Med., 52: 53-58.
Hsü, ST and Yang, P (1957). A preliminary study on blood flukes of cattle and sheep in Kansu Province, including descriptions of a new species. Acta Vet., 2: 117-124.
Karimi, GR; Derakhshanfar, M and Peykari, H (2004). Population density, trematodal infection and ecology of Lymnaea snails in Shadegan, Iran. Arch. Razi Inst., 58: 125-129.
Kruatrachue, M; Bhaibulaya, M and Harinasuta, C (1965). Ornithobilharzia harinasutai sp. nov., a mammalian blood-fluke, its morphology and life-cycle. Ann. Trop. Med. Parasitol., 59: 181-188.
Kumar, V and de Burbure, G (1986). Schistosomes of animals and man in Asia. Helminthol. Abs (Ser A). 55: 469-480.
Li, L; Yu, LY; Zhu, XQ; Wang, CR; Zhai, YQ and Zhao, JP (2008). Orientobilharzia turkestanicum is grouped within African schistosomes based on phylogenetic analyses using sequences of mitochondrial genes. Parasitol. Res., 102: 939-943.
Lockyer, AE; Olson, PD; Ostergaard, P; Rollinson, D; Johnston, DA; Attwood, SW; Southgate, VR; Horak, P; Snyder, SD; Le, TH; Agatsuma, T; McManus, DP; Carmichael, AC; Naem, SN and Littlewood, DTJ (2003). The phylogeny of the Schistosomatidae based on three genes with emphasis on the interrelationships of Schistosoma Weinland, 1858. Parasitology. 126: 203-224.
Mansourian, A (1992). Fresh water snails’ fauna of Iran. Ph.D. Thesis, Tehran Medical Sciences University. P: 76.
Massoud, J (1973). Studies on the schistosomes of domestic animals in Iran: I. Observations on Orientobilharzia turkestanicum (Skrjabin, 1913) in Khuzestan. J. Helminthol., 47: 165-180.
Mirfendereski, R; Karimi, Gh and Paykari, H (2016). Identification of Lymnaea gedrosiana snail as intermediate host of Schistosoma turkestanicum in Boroujerd area. J. Vet. Microbiol., 2: 57-63.
Miya, M and Nishida, M (2000). Use of mitogenomic information in teleostean molecular phylogenetics: a tree-based exploration under the maximumparsimony optimality criterion. Mol. Phylogenet. Evol., 17: 437-455.
Montgomery, RE (1906). Observations on bilharziosis among animals in India. J. Trop. Vet. Sci., 1: 138-174.
Nayebzadeh, H; Kheiri, H and Yavari, Sh (2021). The first report of goat infection with Schistosoma turkestanicum in Lorestan Province, Iran. Vet. Res. Biol. Prod., 34: 208-211.
Sahba, GH and Malek, A (1979). Dermatitis caused by cercariae of Orientobilharzia turkestanicum in the Caspian area of Iran. Am. J. Trop. Med. Hyg., 28: 912-913.
Skrjabin, KI (1913). Schistosomum turkestanicum, new. sp., a new parasite of cattle from Russian Turkestan. Ztschr Infektionskr u Haustier., 13: 457-468.
Snyder, SD and Loker, ES (2000). Evolutionary relationships among the Schistosomatidae (Platyhelminthes: Digenea) and an Asian origin for Schistosoma. J. Parasitol., 86: 283- 288. Soulsby, EJL (1982). Helminths, Arthropods, and Protozoa of domestic animals. 7th Edn., London, Baillière Tindall. PP: 81-83.
Tabaripour, R; Youssefi, MR and Tabaripour, R (2015). Genetic identification of Orientobilharzia turkestanicum from sheep isolates in Iran. Iran J. Parasitol., 10: 62-68.
Tibayrenc, M (2006). The species concept in parasites and other pathogens: a pragmatic approach? Trends Parasitol., 22: 66-70.
Wang, CR; Chen, J; Zhao, JP; Chen, AH; Zhai, YQ; Li, L and Zhu, XQ (2009a). Orientobilharzia species: neglected parasitic zoonotic agents. Acta Trop., 109: 171-175.
Wang, CR; Li, L; Ni, HB; Zhai, YQ; Chen, AH; Chen, J and Zhu, XQ (2009b). Orientobilharzia turkestanicum is a member of Schistosoma genus based on phylogenetic analysis using ribosomal DNA sequences. Exp. Parasitol., 121: 193-197.
Wang, Y; Wang, CR; Zhao, GH; Gao, JF; Li, MW and Zhu, XQ (2011). The complete mitochondrial genome of Orientobilharzia turkestanicum supports its affinity with African Schistosoma spp. Infec. Genet. Evol., 11: 1964-1970.
Webster, BL; Southgate, VR and Littlewood, DT (2006). A revision of the interrelationships of Schistosoma including the recently described Schistosoma guineensis. Int. J. Parasitol., 36: 947-955. | ||
|
آمار تعداد مشاهده مقاله: 254 تعداد دریافت فایل اصل مقاله: 260 |
||