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Effects of propolis supplementation during cryopreservation of ram semen | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 3 - شماره پیاپی 88، 2024، صفحه 250-260 اصل مقاله (970.98 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.49410.7253 | ||
| نویسندگان | ||
| İ. H. Güngör* 1؛ Ş. Özer Kaya1؛ S. Dayan Cinkara1؛ A. Çakır Cihangiroğlu2؛ E. Kaya3؛ T. C. Acısu1؛ F. Erdem Erişir4؛ Ö. Yılmaz3؛ S. Yılmaz3؛ S. Gür1؛ M. Sönmez1؛ G. Türk1 | ||
| 1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey | ||
| 2Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey | ||
| 3Department of Biochemistry, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey | ||
| 4Department of Biology, Faculty of Science, Fırat University, Elazığ, Turkey | ||
| چکیده | ||
| Background: Cryopreservation of ram semen is a very challenging process. Loss of motility during freezing does not allow artificial insemination of rams. Aims: This study aimed to determine whether the inclusion of liquid propolis extract in semen diluents affects the cryopreservation efficiency of ram semen. Methods: Six Akkaraman breed rams were considered for semen study. Semens were combined with Tris+egg yolk extender containing and without (control) propolis at different concentrations (0.25%, 0.50%, 1%, 2%, and 4%). Semen was frozen using routine ram semen freezing procedures. After thawing, motility and kinematic parameters were analyzed by computer assisted semen analysis (CASA), and viability, acrosomal damage level and mitochondrial membrane potential were analyzed by flow-cytometer in all groups. Additionally, fatty acid levels in total semen were analyzed using gas chromatography (GC), and vitamin and cholesterol levels were analyzed using high-performance liquid chromatography (HPLC). In addition, oxidative stress, HOS test, and morphological analyzes were performed after freezing and thawing. Results: The 0.5% propolis group showed a significant increase in total and rapid motility, LIN, membrane integrity, and antioxidant levels compared to the control, and a significant decrease in malondialdehyde (MDA) and low mitochondrial membrane potential (LMMP) levels. Compared to the control, the group containing 4% propolis damaged spermatozoa and caused a significant decrease in total, progressive and rapid motility and high mitochondrial membrane potential (HMMP), Glutathione S-transferase (GST), and catalase (CAT) levels. Conclusion: We showed that adding 0.5% propolis to semen extenders to increase the freezability level of ram semen increases the survival of spermatozoa after freeze-thaw and ensures the success of freezing. | ||
| کلیدواژهها | ||
| CASA؛ Flow-cytometer؛ Propolis liquid extract؛ Ram؛ Semen | ||
| مراجع | ||
|
Abdelnour, SA; Hassan, MA; Shehabeldin, AM; Omar, ME; Khalil, WA and Aman, RM (2023). Effects of propolis-loaded nanoliposomes fortification in extender on buffalo semen cryopreservation. Sci. Rep., 13: 10621.
Aebi, H (1984). Catalase in vitro. Methods in Enzymol., 105: 121-126.
Al-Nawab, N; Al-Dujaily, SS and Al-Obaidi, ZF (2021). Effects of propolis extract medium on in vitro sperms activation of infertile asthenozoospermic men. Ann. Rom. Soc. Cell Biol., 25: 5050-5059.
Bailey, JL; Bilodeau, JF and Cormier, N (2000). Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. J. Androl., 21: 1-7.
Beutler, E (1975). Red cell metabolism: a manual of biochemical methods. In: Halliwell, B (Ed.), Vol. 190 (1), Grune and Starton, New York, Febb Letters. P: 188.
Bucak, MN; Ateşşahin, A and Yüce, A (2008). Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze-thawing process. Small Rumin. Res., 75: 128-134.
Castilho, EFD; Guimarães, JD; Martins, LF; Pinho, RO; Guimarães, SE and Espeschit, CJB (2009). Use of propolis and ascorbic acid on goat semen cryopreservation. Rev. Bras. de Zootec., 38: 2335-2345.
Çevik, M and Tuncer, PB (2005). The effects on the reproductive functions and physico-chemical structure of seminal plasma in domestic animals. Lalahan Hay. Araşt. Enst. Derg., 45: 63-72.
Christie, WW (1990). Gas chromatography and lipids. 1st Edn., Glaskow, The Oil Press.
Deblock-Bostyn, G (1982). The bee and its products. Bull. Soc. Pharm. Lille., 38: 181-203.
Debuyser, E (1983). Propolis. Doctor of Pharmacy Thesis. University of Nantes. France. P: 82.
El-Battawy, KA and Brannas, E (2015). Impact of propolis on cryopreservation of Arctic Charr (Salvelinus alpinus) sperm. Int. J. Vet., 2: 355.
El-Harairy, MA; Khalil, W; Khalifa, EI and Saber, AA (2018). Effect of propolis ethanolic extract supplementation to ram semen extenders on sperm characteristics, lipid peroxidation and some enzymatic activities in seminal plasma in chilled semen. JAPPMU., 9: 235-243.
Ellman, GL; Courtney, KD; Andres, V and Featherstone, RM (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol., 7: 88-95.
El-Seadawy, IES; El-Nattat, WS; El-Tohamy, MM; Aziza, SAH; El-Senosy, YA and Hussein, AS (2017). Preservability of rabbit semen after chilled storage in tris based extender enriched with different concentrations of Propolis ethanolic extract (PEE). Asian Pac. J. Reprod., 6: 68-76.
Forouzanfar, M; Ershad, SF; Hosseini, SM; Hajian, M; Ostad-Hosseini, S; Abid, A; Tavalaee, M; Shahverdi, A; Dizaji, AV and Esfahani, MH (2013). Can permeable super oxide dismutase mimetic agents improve the quality of frozen-thawed ram semen. Cryobiology. 66: 126-130.
Ghisalberti, EL (1979). Propolis: a review. Bee World. 60: 59-84.
Güngör, İH (2023). Evaluation of L-carnosine use in short and long term storage of ram semen by spermatological and flow-cytometric analyses. Ph.D. Thesis, Firat University, Elazig, Turkey.
Güngör, İH; Dayan Cinkara, S; Acısu, TC; Arkalı, G; Koca, RH; Akarsu, SA; Can, C; Özer Kaya, Ş; Kızıl, M; Çakır, A; Fırat, F; Halıcı, MS; Yılmaz, İ; Badıllı, N; Yüce, A; Gür, S; Sönmez, M and Türk, G (2022). Effect of hydrated carbon 60 fullerene on frozen ram semen quality. Biopreserv. Biobank., 20: 340-347.
Habig, WH; Pabst, MJ and Jakoby, WB (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249: 130-139.
Hara, A and Radin, NS (1978). Lipid extraction of tissues with a low-toxicity solvent. Anal. Biochem., 90: 420-426.
Holt, WV; Head, MF and North, RD (1992). Freeze-induced membrane damage in ram spermatozoa is manifested after thawing: observations with experimental cryomicroscopy. Biol. Reprod., 46: 1086-1094.
Jasprica, D; Mornar, A; Debelijak, Z; SmolcicBubalo, A; Medic-Saric, M; Mayer, L; Romic, Z; Bucan, K; Balog, T; Sobocanec, S and Sverko, V (2007). In vivo study of propolis supplementation effects on antioxidative status and red blood cells. J. Ethnopharmacol., 110: 548-554.
Karpinska, J; Mikoluc, B; Motkowski, R and Piotrowska-Jastrzebska, J (2006). HPLC method for simultaneous determination of retinol, alpha-tocopherol and coenzyme Q10 in human plasma. J. Pharm. Biomed. Anal., 18: 232-236.
Katsanidis, E and Addis, PB (1999). Novel HPLC analysis of tocopherols and cholesterol in tissue. Free Radic. Biol. Med., 27: 1137-1140.
Kumazawa, S; Hamasaka, T and Nakayama, T (2004). Antioxidant activity of propolis of various geographic origins. Food Chem., 84: 329-339.
Lahnsteiner, E; Weismann, T and Patzrre, R (1997). Aging processes of rainbow trout semen during storage. Prog. Fish Cult., 59: 272-279.
Lopez-Cervantes, J; Sanchez-Machado, DI and Rios-Vazquez, NJ (2006). High-performance liquid chromato-graphy method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate. J. Chromatogr. A., 1105: 135-139.
Lowry, OH; Rosebrough, NJ; Farr, AL and Randall, RJ (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265-275.
Marti, E; Marti, JI; Muino-Blanco, T and Cebrian-Perez, JA (2008). Effect of the cryopreservation process on the activity and immunolocalization of antioxidant enzymes in ram spermatozoa. J. Androl., 29: 459-467.
Moraes, GV; Mataveli, M; Moura, LPP; Scapinello, C; Mora, F and Osmari, MP (2014). Inclusion of propolis in rabbits’ diets and semen characteristics. Arqvet., 17: 227-231.
Morisawa, M; Suzuki, K; Shimizu, H; Morisawa, S and Yasuda, K (1983). Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes. J. Exp. Biol., 107: 95-103.
Ntemka, A; Tsakmakidis, I; Kiossis, E; Milovanovic, A and Boscos, C (2018). Current status and advances in ram semen cryopreservation. J. Hellenic Vet. Med. Soc., 69: 911-924.
Öğretmen, F; Inanan, BE and Öztürk, M (2014). Protective effects of propolis on cryopreservation of common carp (Cyprinus carpio) sperm. Cryobiology. 68: 107-112.
Özer Kaya, Ş; Güngör, İH; Cinkara, SD; Acisu, TC; Koca, RH; Akarsu, SA; Can, C; Çakır, A; Yılmaz, İ; Halıcı, MS; Gür, S; Sönmez, M and Türk, G (2021). Effect of different doses of hydrated C60 fullerene nanoparticles on ram semen duringcool storage. Turkish J. Vet. Anim. Sci., 45: 139-147.
Paulino, N; Abreu, S; Uto, Y; Koyama, D; Nagasawa, H; Hori, H; Dirsch, V; Vollmar, A; Scremin, A and Bretz, W (2008). Antiinflammatory effects of a bioavailable compound, Artepillin C, in Brazilian propolis. Eur. J. Pharmacol., 587: 296-301.
Placer, ZA; Cushman, L and Johnson, BC (1966). Estimation of products of lipid peroxidation in biological fluids. Anal. Biochem., 16: 359-364. Rizk, SM; Zaki, HF and Mina, MA (2014). Propolis attenuates doxorubicin-induced testicular toxicity in rats. Food Chem. Toxicol., 67: 176-186.
Sanchez-Machado, DI; Lopez-Hernandez, J; Paseiro-Losada, P and Lopez-Cervantes, J (2004). An HPLC method for the quantification of sterols in edible seaweeds. Biomed. Chromatogr., 18: 183-190.
Selem, ASMA (2012). Effect of propolis on ruminal fermentation, reproductive and productive performance of Santa Ines ewes. Ph.D. Thesis, Universidade de São Paulo.
Simoes, L; Gregório, L; da Silva Filho, A; de Souza, M; Azzolini, A; Bastos, J and Lucisano-Valim, Y (2004). Effect of Brazilian green propolis on the production of reactive oxygen species by stimulated neutrophils. J. Ethnopharmacol., 94: 59-65.
Sun, Y; Oberly, LW and Ying, LA (1988). Simple method for clinical assay of superoxide dismutase. Clin. Chem., 34: 497-500.
Türk, G (2015). Physiological and pathological effects of reactive oxygen species on spermatozoon functions. Turkiye Klinikleri J. Reprod. Artif Insemin-Special Topics. 1: 26-34.
Walker, P and Crane, E (1987). Constituents of propolis. Apidologie. 18: 327-334.
Warnecke, D and Pluta, HJ (2003). Motility and fertilization capacity of frozen/thawed common carp (Cyprinus carpio L.) sperm using dimethyl-acetamide as the main cryoprotectant. Aquaculture. 215: 167-185.
Yeni, D; Gülhan, MF; İnanç, ME; Avdatek, F; Güngör, Ş; Türkmen, R; Tuncer, PB and Tasdemir, U (2022). The cryoprotective potential of propolis supplemented in frozen-thawed bull semen: biochemical and physiological findings. Pol. J. Vet. Sci., 25: 5-12.
Zaghloul, A; El-Hamid, I and Khalifa, M (2016). Effect of supplementing ram semen diluent with propolis extract on chilled preservation capacity of sperm. Egypt J. Sheep Goats Sci., 11: 147-155. | ||
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