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افتخارآفرینی دبیران مجلات علمی دانشگاه شیراز در عرصۀ بین‌المللی
اطلاعیه برگزاری دوره آموزشی هوش مصنوعی: SWOT و سیاست‌های ناشران بین‌المللی در تاریخ 19 خرداد 1404
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Dana, Fatemeh, Mahjoub, Soleiman, Shokati Eshkiki, Zahra, Namazi, Abolfazl, Tabaeian, Seidamir Pasha, Akbari, Abolfazl. (1404). Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients. , 14(2), 157-166. doi: 10.22099/mbrc.2025.51654.2061
Fatemeh Dana; Soleiman Mahjoub; Zahra Shokati Eshkiki; Abolfazl Namazi; Seidamir Pasha Tabaeian; Abolfazl Akbari. "Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients". , 14, 2, 1404, 157-166. doi: 10.22099/mbrc.2025.51654.2061
Dana, Fatemeh, Mahjoub, Soleiman, Shokati Eshkiki, Zahra, Namazi, Abolfazl, Tabaeian, Seidamir Pasha, Akbari, Abolfazl. (1404). 'Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients', , 14(2), pp. 157-166. doi: 10.22099/mbrc.2025.51654.2061
Dana, Fatemeh, Mahjoub, Soleiman, Shokati Eshkiki, Zahra, Namazi, Abolfazl, Tabaeian, Seidamir Pasha, Akbari, Abolfazl. Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients. , 1404; 14(2): 157-166. doi: 10.22099/mbrc.2025.51654.2061

Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients

Molecular Biology Research Communications
دوره 14، شماره 2، 2025، صفحه 157-166    اصل مقاله (572.49 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2025.51654.2061
نویسندگان
Fatemeh Dana1، 2؛ Soleiman Mahjoub* 1، 3؛ Zahra Shokati Eshkiki4؛ Abolfazl Namazi5، 6؛ Seidamir Pasha Tabaeian5، 6؛ Abolfazl Akbari* 6
1Department of Clinical Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, Iran
2Student Research Committee, Babol University of Medical Sciences, Babol, Iran
3Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
4Alimentary Tract Research Center, Clinical Sciences Research Institute, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5Department of Internal Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
6Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
چکیده
The expression level of exosomal long non-coding RNAs (lncRNAs) can be relevant for clinical diagnostic approaches. The object of our study was to evaluate the differential expression of lncRNAs colon cancer associated transcript 1 (CCAT1) and X-inactive specific transcript (XIST) in plasma exosomes of colorectal cancer (CRC) patients and investigate their potential as clinical biomarkers. In a case-control study, 62 CRC patients and 62 healthy persons were studied. Plasma exosomes were isolated by a centrifugation approach and were characterized by microscopy and western blotting. After RNA extraction and cDNA synthesis, using real-time PCR technique, the relative expression of lncRNAs was evaluated. The expression levels of lncRNA CCAT1, but not XIST, were meaningfully increased in the plasma-derived exosomes of CRC patients compared to non-cancer individuals (p= 0.001, 0.083 respectively). Further analyses revealed that the expression levels of exosomal lncRNA CCAT1 were associated with the lymphovascular invasion and tumor differentiation (p<0.05). ROC curve analysis documented a diagnostic power for lncRNA CCAT1 in CRC with a sensitivity of 79% and a specificity of 80% with an optimal cutoff point 6.5, with an area under curve (AUC)=86% and p<0.0001. Also, lncRNA XIST revealed a sensitivity of 62% and a specificity of 61% with a cutoff point 2.4, with an AUC=65%. Our findings indicated the potential of plasma-derived exosomal lncRNA CCAT1 as a non-invasive clinical indicator for the diagnosis of CRC patients.
کلیدواژه‌ها
Colorectal cancer؛ Long non-coding RNAs؛ LncRNA؛ XIST؛ CCAT1؛ Exosome
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