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Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients | ||
| Molecular Biology Research Communications | ||
| دوره 14، شماره 2، 2025، صفحه 157-166 اصل مقاله (572.49 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2025.51654.2061 | ||
| نویسندگان | ||
| Fatemeh Dana1، 2؛ Soleiman Mahjoub* 1، 3؛ Zahra Shokati Eshkiki4؛ Abolfazl Namazi5، 6؛ Seidamir Pasha Tabaeian5، 6؛ Abolfazl Akbari* 6 | ||
| 1Department of Clinical Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, Iran | ||
| 2Student Research Committee, Babol University of Medical Sciences, Babol, Iran | ||
| 3Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran | ||
| 4Alimentary Tract Research Center, Clinical Sciences Research Institute, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran | ||
| 5Department of Internal Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 6Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| The expression level of exosomal long non-coding RNAs (lncRNAs) can be relevant for clinical diagnostic approaches. The object of our study was to evaluate the differential expression of lncRNAs colon cancer associated transcript 1 (CCAT1) and X-inactive specific transcript (XIST) in plasma exosomes of colorectal cancer (CRC) patients and investigate their potential as clinical biomarkers. In a case-control study, 62 CRC patients and 62 healthy persons were studied. Plasma exosomes were isolated by a centrifugation approach and were characterized by microscopy and western blotting. After RNA extraction and cDNA synthesis, using real-time PCR technique, the relative expression of lncRNAs was evaluated. The expression levels of lncRNA CCAT1, but not XIST, were meaningfully increased in the plasma-derived exosomes of CRC patients compared to non-cancer individuals (p= 0.001, 0.083 respectively). Further analyses revealed that the expression levels of exosomal lncRNA CCAT1 were associated with the lymphovascular invasion and tumor differentiation (p<0.05). ROC curve analysis documented a diagnostic power for lncRNA CCAT1 in CRC with a sensitivity of 79% and a specificity of 80% with an optimal cutoff point 6.5, with an area under curve (AUC)=86% and p<0.0001. Also, lncRNA XIST revealed a sensitivity of 62% and a specificity of 61% with a cutoff point 2.4, with an AUC=65%. Our findings indicated the potential of plasma-derived exosomal lncRNA CCAT1 as a non-invasive clinical indicator for the diagnosis of CRC patients. | ||
| کلیدواژه‌ها | ||
| Colorectal cancer؛ Long non-coding RNAs؛ LncRNA؛ XIST؛ CCAT1؛ Exosome | ||
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