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Dana, Fatemeh, Mahjoub, Soleiman, Shokati Eshkiki, Zahra, Namazi, Abolfazl, Tabaeian, Seidamir Pasha, Akbari, Abolfazl. (1404). Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients. , 14(2), 157-166. doi: 10.22099/mbrc.2025.51654.2061
Fatemeh Dana; Soleiman Mahjoub; Zahra Shokati Eshkiki; Abolfazl Namazi; Seidamir Pasha Tabaeian; Abolfazl Akbari. "Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients". , 14, 2, 1404, 157-166. doi: 10.22099/mbrc.2025.51654.2061
Dana, Fatemeh, Mahjoub, Soleiman, Shokati Eshkiki, Zahra, Namazi, Abolfazl, Tabaeian, Seidamir Pasha, Akbari, Abolfazl. (1404). 'Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients', , 14(2), pp. 157-166. doi: 10.22099/mbrc.2025.51654.2061
Dana, Fatemeh, Mahjoub, Soleiman, Shokati Eshkiki, Zahra, Namazi, Abolfazl, Tabaeian, Seidamir Pasha, Akbari, Abolfazl. Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients. , 1404; 14(2): 157-166. doi: 10.22099/mbrc.2025.51654.2061

Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients

Molecular Biology Research Communications
دوره 14، شماره 2، 2025، صفحه 157-166    اصل مقاله (572.49 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2025.51654.2061
نویسندگان
Fatemeh Dana1، 2؛ Soleiman Mahjoub* 1، 3؛ Zahra Shokati Eshkiki4؛ Abolfazl Namazi5، 6؛ Seidamir Pasha Tabaeian5، 6؛ Abolfazl Akbari* 6
1Department of Clinical Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, Iran
2Student Research Committee, Babol University of Medical Sciences, Babol, Iran
3Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
4Alimentary Tract Research Center, Clinical Sciences Research Institute, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5Department of Internal Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
6Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
چکیده
The expression level of exosomal long non-coding RNAs (lncRNAs) can be relevant for clinical diagnostic approaches. The object of our study was to evaluate the differential expression of lncRNAs colon cancer associated transcript 1 (CCAT1) and X-inactive specific transcript (XIST) in plasma exosomes of colorectal cancer (CRC) patients and investigate their potential as clinical biomarkers. In a case-control study, 62 CRC patients and 62 healthy persons were studied. Plasma exosomes were isolated by a centrifugation approach and were characterized by microscopy and western blotting. After RNA extraction and cDNA synthesis, using real-time PCR technique, the relative expression of lncRNAs was evaluated. The expression levels of lncRNA CCAT1, but not XIST, were meaningfully increased in the plasma-derived exosomes of CRC patients compared to non-cancer individuals (p= 0.001, 0.083 respectively). Further analyses revealed that the expression levels of exosomal lncRNA CCAT1 were associated with the lymphovascular invasion and tumor differentiation (p<0.05). ROC curve analysis documented a diagnostic power for lncRNA CCAT1 in CRC with a sensitivity of 79% and a specificity of 80% with an optimal cutoff point 6.5, with an area under curve (AUC)=86% and p<0.0001. Also, lncRNA XIST revealed a sensitivity of 62% and a specificity of 61% with a cutoff point 2.4, with an AUC=65%. Our findings indicated the potential of plasma-derived exosomal lncRNA CCAT1 as a non-invasive clinical indicator for the diagnosis of CRC patients.
کلیدواژه‌ها
Colorectal cancer؛ Long non-coding RNAs؛ LncRNA؛ XIST؛ CCAT1؛ Exosome
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مراجع
  1. Siegel RL, Miller KD, Wagle NS, Jemal A. Cancer statistics, 2023. CA Cancer J Clin 2023;73:17-48.
  2. Cardenas C, Abrishami S. Colorectal carcinoma: Review and novelties in 2023. Clin Oncol Case Rep 2023;6:2.
  3. Karamzadeh AR, Heidari M, Namazi A, Tabaeian SP, Akbari A. The dysregulation and clinical relevance of lncRNAs MYOSLID and SFTA1P in colorectal cancer patients. Mol Biol Rep 2024;51:1109.
  4. Akbari A, Mobini GR, Maghsoudi R, Akhtari J, Faghihloo E, Farahnejad Z. Modulation of transforming growth factor‑β signaling transducers in colon adenocarcinoma cells induced by staphylococcal enterotoxin B. Mol Med Rep 2016;13:909-914.
  5. Tepus M, Yau TO. Non-invasive colorectal cancer screening: an overview. Gastrointest Tumors 2020;7:62-73.
  6. Ferrari A, Neefs I, Hoeck S, Peeters M, Van Hal G. Towards novel non-invasive colorectal cancer screening methods: a comprehensive review. Cancers (Basel) 2021;13:1820.
  7. Tabaeian SP, Eshkiki ZS, Dana F, Fayyaz F, Baniasadi M, Agah S, Masoodi M, Safari E, Sedaghat M, Abedini P, Akbari A. Evaluation of tumor-educated platelet long non-coding RNAs (lncRNAs) as potential diagnostic biomarkers for colorectal cancer. J Cancer Res Ther 2024:20:1453-1458.
  8. Agah S, Akbari A, Talebi A, Masoudi M, Sarveazad A, Mirzaei A, Nazmi F. Quantification of plasma cell-free circulating DNA at different stages of colorectal cancer. Cancer Invest 2017;35:625-632.
  9. Molnár B, Galamb O, Kalmár A, Barták BK, Nagy ZB, Tóth K, Tulassay Z, Igaz P, Dank M. Circulating cell-free nucleic acids as biomarkers in colorectal cancer screening and diagnosis-an update. Expert Rev Mol Diagn 2019;19:477-498.
  10. Toth K, Bartak BK, Tulassay Z, Molnar B. Circulating cell-free nucleic acids as biomarkers in colorectal cancer screening and diagnosis. Expert Rev Mol Diagn 2016;16:239-252.
  11. Thakur K, Singh MS, Feldstein-Davydova S, Hannes V, Hershkovitz D, Tsuriel S. Extracellular vesicle-derived DNA vs. cfDNA as a biomarker for the detection of colon cancer. Genes (Basel) 2021;12:1171.
  12. Ruivo CF, Adem B, Silva M, Melo SA. The biology of cancer exosomes: insights and new perspectives. Cancer Res 2017;77:6480-6488.
  13. Kalluri R. The biology and function of exosomes in cancer. J Clin Invest 2016;126:1208-1215.
  14. Kok VC, Yu CC. Cancer-derived exosomes: their role in cancer biology and biomarker development. Int J Nanomedicine 2020;15:8019-8036.
  15. Boriachek K, Islam MN, Möller A, Salomon C, Nguyen NT, Hossain MSA, Yamauchi Y, Shiddiky MJA. Biological functions and current advances in isolation and detection strategies for exosome nanovesicles. Small 2018;14: doi: 10.1002/smll.201702153. 
  16. LeBleu VS, Kalluri R. Exosomes as a multicomponent biomarker platform in cancer. Trends Cancer 2020;6:767-774.
  17. Hon KW, Abu N, Ab Mutalib NS, Jamal R. Exosomes as potential biomarkers and targeted therapy in colorectal cancer: a mini-review. Front Pharmacol 2017;8:583.
  18. Baassiri A, Nassar F, Mukherji D, Shamseddine A, Nasr R, Temraz S. Exosomal non coding RNA in LIQUID biopsies as a promising biomarker for colorectal cancer. Int J Mol Sci 2020;21:1398.
  19. Wu Q, Liu W, Wang J, Zhu L, Wang Z, Peng Y. Exosomal noncoding RNAs in colorectal cancer. Cancer Lett 2020;493:228-235.
  20. Deng H, Wang JM, Li M, Tang R, Tang K, Su Y, Hou Y, Zhang J. Long non-coding RNAs: New biomarkers for prognosis and diagnosis of colon cancer. Tumour Biol 2017;39: 1010428317706332.
  21. Saus E, Brunet-Vega A, Iraola-Guzman S, Pegueroles C, Gabaldon T, Pericay C. Long non-coding RNAs as potential novel prognostic biomarkers in colorectal cancer. Front Genet 2016;7:54.
  22. Abedini P, Fattahi A, Agah S, Talebi A, Beygi AH, Amini SM, Mirzaei A, Akbari A. Expression analysis of circulating plasma long noncoding RNAs in colorectal cancer: The relevance of lncRNAs ATB and CCAT1 as potential clinical hallmarks. J Cell Physiol 2019;234:22028-22033.
  23. Talebi A, Azizpour M, Agah S, Masoodi M, Mobini GR, Akbari A. The relevance of long noncoding RNAs in colorectal cancer biology and clinical settings. J Cancer Res Ther 2020; 16(Suppl 1):S22-S33.
  24. Akbari A, Abbasi S, Borumandnia N, Eshkiki ZS, Sedaghat M, Tabaeian SP, Faghihi Kashani A, Talebi A. Epigenetic regulation of gastrointestinal cancers mediated by long non-coding RNA. Cancer Biomark 2022;35:359-377.
  25. Zhao Y, Song X, Song X, Xie L. Identification of Diagnostic Exosomal LncRNA-miRNA-mRNA Biomarkers in Colorectal Cancer Based on the ceRNA Network. Pathol Oncol Res 2022;28:1610493.
  26. Xiao Y, Zhong J, Zhong B, Huang J, Jiang L, Jiang Y, Yuan J, Sun J, Dai L, Yang C, Li Z, Wang J, Zhong T. Exosomes as potential sources of biomarkers in colorectal cancer. Cancer Lett 2020;476:13-22.
  27. Liau XL, Salvamani S, Gunasekaran B, Chellappan DK, Rhodes A, Ulaganathan V, Tiong YL. CCAT 1-a pivotal oncogenic long non-coding RNA in colorectal cancer. Br J Biomed Sci 2023;80:11103.
  28. Nissan A, Stojadinovic A, Mitrani‐Rosenbaum S, Halle D, Grinbaum R, Roistacher M, Bochem A, Dayanc BE, Ritter G, Gomceli I, Bostanci EB, Akoglu M, Chen YT, Old LJ, Gure AO. Colon cancer associated transcript‐1: a novel RNA expressed in malignant and pre‐malignant human tissues. Int J Cancer 2012;130:1598-1606.
  29. Wang L, Cho KB, Li Y, Tao G, Xie Z, Guo B. Long noncoding RNA (lncRNA)-mediated competing endogenous RNA networks provide novel potential biomarkers and therapeutic targets for colorectal cancer. Int J Mol Sci 2019;20:5758.
  30. Dastmalchi N, Safaralizadeh R, Nargesi MM. LncRNAs: potential novel prognostic and diagnostic biomarkers in colorectal cancer. Curr Med Chem 2020;27:5067-5077.
  31. Lan F, Zhang X, Li H, Yue X, Sun Q. Serum exosomal lncRNA XIST is a potential non‐invasive biomarker to diagnose recurrence of triple‐negative breast cancer. J Cell Mol Med 2021;25:7602-7607.
  32. Omrani Tabarestani F, Akbari A, Zare Karizi S, Sotoodehnejadnematalahi F. Regulation of long non-coding RNAs XIST and ROR induced by homeodomain protein TGIF2LX in colorectal cancer. J Cancer Res Ther 2022;18(Suppl 2):S359-S366.
  33. Crotti S, Agnoletto E, Cancemi G, Di Marco V, Traldi P, Pucciarelli S, Nitti D, Agostini M. Altered plasma levels of decanoic acid in colorectal cancer as a new diagnostic biomarker. Anal Bioanal Chem 2016;408:6321-6328.
  34. Zygulska AL, Pierzchalski P. Novel diagnostic biomarkers in colorectal cancer. Int J Mol Sci 2022;23:852.
  35. Hauptman N, Glavač D. Colorectal cancer blood-based biomarkers. Gastroenterol Res Pract 2017;2017:2195361.
  36. Hu D, Zhan Y, Zhu K, Bai M, Han J, Si Y, Zhang H, Kong D. Plasma exosomal long non-coding RNAs serve as biomarkers for early detection of colorectal cancer. Cell Physiol Biochem 2018;51:2704-2715.
  37. Rizk NI, Abulsoud AI, Kamal MM, Kassem DH, Hamdy NM. Exosomal-long non-coding RNAs journey in colorectal cancer: Evil and goodness faces of key players. Life Sci 2022; 292:120325.
  38. Ye Z, Zhou M, Tian B, Wu B, Li J. Expression of lncRNA-CCAT1, E-cadherin and N-cadherin in colorectal cancer and its clinical significance. Int J Clin Exp Med 2015;8:3707-3715.
  39. He X, Tan X, Wang X, Jin H, Liu L, Ma L, Yu H, Fan Z. C-Myc-activated long noncoding RNA CCAT1 promotes colon cancer cell proliferation and invasion. Tumour Biol 2014;35: 12181-12188.
  40. Arunkumar G, Murugan AK, Prasanna Srinivasa Rao H, Subbiah S, Rajaraman R, Munirajan AK. Long non-coding RNA CCAT1 is overexpressed in oral squamous cell carcinomas and predicts poor prognosis. Biomed Rep 2017;6:455-462.
  41. Xiao K, Dong Z, Wang D, Liu M, Ding J, Chen W, Shang Z, Yue C, Zhang Y. Clinical value of lncRNA CCAT1 in serum extracellular vesicles as a potential biomarker for gastric cancer. Oncol Lett 2021;21:447.
  42. Zhang XF, Liu T, Li Y, Li S. Overexpression of long non-coding RNA CCAT1 is a novel biomarker of poor prognosis in patients with breast cancer. Int J Clin Exp Pathol 2015;8: 9440-9445.
  43. Jiang X, Li ZL, Li JL, Zheng WY, Li XH, Cui YF, Sun DJ. LncRNA CCAT1 as the unfavorable prognostic biomarker for cholangiocarcinoma. Eur Rev Med Pharmacol Sci 2017;21:1242-1247.
  44. Zong D, Liu X, Li J, Long Y, Ouyang R, Chen Y. LncRNA-CCAT1/miR-152-3p is involved in CSE-induced inflammation in HBE cells via regulating ERK signaling pathway. Int Immunopharmacol 2022;109:108818.
  45. Gharib E, Nazemalhosseini‐Mojarad E, Baghdar K, Nayeri Z, Sadeghi H, Rezasoltani S, Jamshidi-Fard A, Larki P, Sadeghi A, Hashemi M, Asadzadeh Aghdaei H. Identification of a stool long non‐coding RNAs panel as a potential biomarker for early detection of colorectal cancer. J Clin Lab Anal 2021;35:e23601.
  46. Iranmanesh H, Entezari M, Rejali L, Nazemalhosseini-Mojarad E, Maghsoudloo M, Asadzadeh Aghdaei H, Zali MR, Hushmandi K, Rabiee N, Makvandi P, Ashrafizadeh M, Hashemi M. The association of clinicopathological characterizations of colorectal cancer with membrane-bound mucins genes and LncRNAs. Pathol Res Pract 2022;233:153883.
  47. Li C, Wan L, Liu Z, Xu G, Wang S, Su Z, Zhang Y, Zhang C, Liu X, Lei Z, Zhang HT. Long non-coding RNA XIST promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-367/141-ZEB2 axis in non-small-cell lung cancer. Cancer Lett 2018; 418:185-195.
  48. Chen DL, Chen LZ, Lu YX, Zhang DS, Zeng ZL, Pan ZZ, Huang P, Wang FH, Li YH, Ju HQ, Xu RH. Long noncoding RNA XIST expedites metastasis and modulates epithelial–mesenchymal transition in colorectal cancer. Cell Death Dis 2017;8:e3011.
  49. Zheng H, Zhang M, Ke X, Deng X, Li D, Wang Q, Yan S, Xue Y, Wang Q. LncRNA XIST/miR-137 axis strengthens chemo-resistance and glycolysis of colorectal cancer cells by hindering transformation from PKM2 to PKM1. Cancer Biomark 2021;30:395-406.
  50. Yu J, Dong W, Liang J. Extracellular vesicle-transported long non-coding RNA (LncRNA) X inactive-specific transcript (XIST) in serum is a potential novel biomarker for colorectal cancer diagnosis. Med Sci Monit 2020;26:e924448-e924451.
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