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Effects of major OMPs and LPS of Brucella on the control of activation of bone marrow-derived dendritic cells and proliferation of T-lymphocytes in mice | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 3 - شماره پیاپی 88، 2024، صفحه 224-232 اصل مقاله (1.18 M) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.48969.7177 | ||
| نویسندگان | ||
| Z. Xu1؛ Z. Tong1؛ H. Zhang1؛ X. Deng1؛ N. Yang1؛ Z. Wang2؛ C. Chen* 2 | ||
| 1Graduated from School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China | ||
| 2Department of Basic Veterinary Medicine, School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China | ||
| چکیده | ||
| Background: Brucella outer membrane proteins (OMPs) are highly immunogenic, and lipopolysaccharides (LPS) are also considered significant antigens, making them potential candidates for subunit vaccines. Aims: To investigate the effects of Brucella OMPs and LPSs on mouse bone marrow-derived dendritic cells (BMDC) activation and T-lymphocyte proliferation. Methods: BMDC were isolated and cultured in vitro, and subsequently co-cultured with Brucella recombinant proteins (rOMP10, rOMP19, rBP26, rOMP25, and rOMP31), as well as smooth LPS (S-LPS) or rough LPS (R-LPS). The expression of maturation markers on the surface of BMDCs was determined using flow cytometry, while the expression of TLR receptors was determined using RT-PCR. The levels of inflammatory cytokines were measured using iELISA, and the impact on the proliferation of mouse T-lymphocytes was assessed using the MTT method. Results: The impact of LPS on BMDC maturation, TLRs-mediated cytokine secretion, and antigen presentation was found to be limited. In contrast, rOMP10, rOMP19, and rBP26 were observed to promote BMDC maturation, increase the expression of TLR-2 and TLR-4 mRNA, and activate T-lymphocyte proliferation by significantly increasing the expression of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-6, IL-12) and antigen-presenting molecules. However, rOMP25 and rOMP31 did not promote BMDC maturation, inhibited the expression of MHCI and MHCII antigen-presenting molecules, and increased the expression of inflammation-suppressing cytokines (IL-10 and IL-4), resulting in the inhibition of T-lymphocyte proliferation. Conclusion: Brucella OMP10, OMP19, and BP26 play an important role in activating the host immune response, while OMP25 together with OMP31 may play a role in Brucella immune escape. | ||
| کلیدواژهها | ||
| Antigen presentation؛ Brucella OMPs؛ Dendritic cell maturation؛ LPS؛ T-lymphocyte proliferation | ||
| مراجع | ||
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