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Cloning and expression of Fusion and Hemagglutinin proteins of peste des petits ruminants virus in the baculovirus system: an immunogenicity study in mice | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 3 - شماره پیاپی 88، 2024، صفحه 210-215 اصل مقاله (401.46 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.48368.7047 | ||
| نویسندگان | ||
| M. Taheri Asl1؛ M. Moghbeli* 2؛ M. Kargar3، 4؛ M. Lotfi5؛ F. Kafilzadeh4 | ||
| 1Ph.D. Student in Microbiology, Department of Microbiology, College of Science, Jahrom Branch, Islamic Azad University, Jahrom, Iran | ||
| 2Department of Biology, College of Science, Damghan Branch, Islamic Azad University, Damghan, Iran | ||
| 3Department of Biology, Zand Institute of Higher Education, Shiraz, Iran (current address) | ||
| 4Department of Microbiology, College of Science, Jahrom Branch, Islamic Azad University, Jahrom, Iran | ||
| 5Department of Quality Control, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| Background: Peste des petits ruminants virus (PPRV) is one of the most economically important pathogens in sheep and goats. Fusion (F) and Hemagglutinin (H) proteins are the main immune-stimulating antigens. Aims: The present study aimed to clone and express F and H genes in baculovirus, and evaluate the immunogenicity of recombinant proteins produced by sf9 cells in mice. Methods: Amplified F and H genes (by RT-PCR using specific primers) were cloned into pFastBac Dual plasmid. The recombinant plasmid was transformed in DH10Bac host cells. SDS-PAGE and Western blotting was performed to control the recombinant protein, and a whole pure and specific recombinant protein was obtained. Results: The immunogenicity of 20 μg of non-adjuvant recombinant proteins in Balb-c mice showed better results compared with the attenuated PPR vaccine. Conclusion: The recombinant protein obtained from this study can be a suitable candidate for the production of recombinant vaccines against PPRV. | ||
| کلیدواژهها | ||
| pFastBac Dual؛ PPRV؛ Recombinant vaccine؛ sf9 cell | ||
| مراجع | ||
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