| تعداد نشریات | 24 |
| تعداد شمارهها | 881 |
| تعداد مقالات | 7,867 |
| تعداد مشاهده مقاله | 14,699,811 |
| تعداد دریافت فایل اصل مقاله | 12,550,017 |
Cloning and expression of Fusion and Hemagglutinin proteins of peste des petits ruminants virus in the baculovirus system: an immunogenicity study in mice | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 3 - شماره پیاپی 88، 2024، صفحه 210-215 اصل مقاله (401.46 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.48368.7047 | ||
| نویسندگان | ||
| M. Taheri Asl1؛ M. Moghbeli* 2؛ M. Kargar3، 4؛ M. Lotfi5؛ F. Kafilzadeh4 | ||
| 1Ph.D. Student in Microbiology, Department of Microbiology, College of Science, Jahrom Branch, Islamic Azad University, Jahrom, Iran | ||
| 2Department of Biology, College of Science, Damghan Branch, Islamic Azad University, Damghan, Iran | ||
| 3Department of Biology, Zand Institute of Higher Education, Shiraz, Iran (current address) | ||
| 4Department of Microbiology, College of Science, Jahrom Branch, Islamic Azad University, Jahrom, Iran | ||
| 5Department of Quality Control, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| Background: Peste des petits ruminants virus (PPRV) is one of the most economically important pathogens in sheep and goats. Fusion (F) and Hemagglutinin (H) proteins are the main immune-stimulating antigens. Aims: The present study aimed to clone and express F and H genes in baculovirus, and evaluate the immunogenicity of recombinant proteins produced by sf9 cells in mice. Methods: Amplified F and H genes (by RT-PCR using specific primers) were cloned into pFastBac Dual plasmid. The recombinant plasmid was transformed in DH10Bac host cells. SDS-PAGE and Western blotting was performed to control the recombinant protein, and a whole pure and specific recombinant protein was obtained. Results: The immunogenicity of 20 μg of non-adjuvant recombinant proteins in Balb-c mice showed better results compared with the attenuated PPR vaccine. Conclusion: The recombinant protein obtained from this study can be a suitable candidate for the production of recombinant vaccines against PPRV. | ||
| کلیدواژهها | ||
| pFastBac Dual؛ PPRV؛ Recombinant vaccine؛ sf9 cell | ||
| مراجع | ||
|
Baazizi, R; Mahapatra, M; Donald Clarke, B; Ait-Oudhia, K; Khelef, D and Parida, S (2017). Peste des petits ruminants (PPR): A neglected tropical disease in Maghreb region of North Africa and its threat to Europe. PLOS One. 12: 1-16.
Cotmore, SF; Agbandje-Mckenna, M; Canuti, M; Chiorini, JA; Eis-Hubinger, AM; Hughes, J; Mietzsch, M; Modha, S; Ogliastro, M; Penzes, JJ; Pintel, DJ; Qiu, J; Soderlund-Venermo, M; Tattersall, P and Tijssen, P (2019). ICTV virus taxonomy profile: Parvoviridae. J. Gen. Virol., 100: 367-368.
Fakri, F; Bamouh, Z; Ghazal, F; Baha, W; Tadlaoui, K; Fassi Fihri, O; Chen, W; Bu, Z and Elharrak, M (2018). Comparative evaluation of three capripoxvirus-vectored peste des petits ruminants vaccines. Virology. 514: 211-215.
Fakri, F; Ghazal, F; Daouam, S; Elarkam, A; Douieb, L; Zouheir, Y; Tadlaoui, K and Fassi-Fihri, O (2015). Development and field application of a new combined vaccine against peste des petits ruminants and sheep pox. J. Trivac., 4: 33-37.
Gauger, PC and Vincent, AL (2020). Serum virus neutralization assay for detection and quantitation of serum neutralizing antibodies to influenza a virus in Swine. Methods Mol. Biol., 2123: 321-333.
He, F (2011). Bradford protein assay. Bio. Protoc., 1: 1-2.
Kumar, N; Barua, S; Riyesh, T and Tripathi, BN (2017). Advances in peste des petits ruminants vaccines. Vet. Microbiol., 206: 91-101.
Liu, F; Wu, X; Li, L and Wang, Z (2014). Budding of peste des petits ruminants virus-like particles from insect cell membrane based on intracellular co-expression of peste des petits ruminants virus M, H and N proteins by recombinant baculoviruses. J. Virol. Methods. 207: 78-85.
Luckow, VL and Summers, MD (1988). Trends in the development of baculovirus expression vectors. Nat. Biotechnol., 6: 47-55.
Marashi, M; Masudi, S; Kharazian Moghdam, M; Modirrousta, H; Marashi, M; Parvizifar, M; Dargi Saljooghian, M; Homan, F; Hoffmann, B; Schulz, C; Starick, E; Beer, M and Fereidouni, S (2017). Peste des petits ruminants virus in vulnerable wild small ruminants, Iran, 2014-2016. Emerg Infects Dis., 23: 704-706.
Mokhtari, A; Azizi, Z and Rabiaee Fradonbeh, S (2017). Epidemiological study and spatial modeling of peste des petits ruminants (PPR) in central area of Iran. REV. MVZ. Cordoba., 22: 5899-5909.
Niyokwishimira, A; Baziki, J; Dundon, WG; Nwankpa, N; Njoroge, C; Boussini, H; Wamwayi, H; Jaw, B; Cattoli, G; Nkundwanayu, C; Ntakirutimana, D; Balikowa, D; Nyabongo, L; Zhang, Z and Charles Bodjo, S (2019). Detection and molecular characterization of Peste des Petits Ruminants virus from outbreaks in Burundi, December 2017-January 2018. Transbound Emerg Dis., 66: 2067-2073.
OIE • (Office International des Epizooties of the World Organization for Animal Health) 12, rue de Prony • (no date).
Patel, J; Patel, DH; Mavadiya, S and Solanki, JB (2015). Clinicopathological investigation of an outbreak of peste des petits ruminants in small ruminants in South Gujarat, India. Indian J. Vet. Pathol., 39: 20.
Pruvot, M; Fine, AE; Hollinger, C; Strindberg, S; Damdinjav, B; Buuveibaatar, B; Chimeddorj, B; Bayandonoi, G; Khishgee, B; Sandag, B; Narmandakh, J; Jargalsaikhan, T; Bataa, B; McAloose, D; Shatar, M; Basan, G; Mahapatra, M; Selvaraj, M; Satya, P; Njeumi, F; Kock, R and Shiilegdamba, E (2020). Outbreak of peste des petits ruminants among critically endangered Mongolian saiga and other wild ungulates, Mongolia, 2016-2017. Emerg. Infect. Dis., 26: 51-62.
Rahman, MZ; Haider, N; Gurley, ES; Ahmed, S; Osmani, MG; Hossain, MB; Islam, A; Akhan, S; Hossain, ME; Epstein, JH; Zeidner, N and Rahman, M (2018). Epidemiology and genetic characterization of Peste des petits ruminants virus in Bangladesh. Vet. Med. Sci., 4: 161-171.
Sambrook, J and Russell, DW (2006). Preparation and transformation of competent E. coli using calcium chloride. CSH Protoc., 2006: 1-10.
Sambrook, J and Russell, DW (2012). Molecular cloning: A laboratory manual. 4th Edn., New York, CSH Press. PP: 1483-1634.
Schägger, H (2006). Tricine-SDS-PAGE. Nat Protoc., 1: 16-22.
Tounkara, K; Kwiatek, O; Niang, M; Sidibe, C; Sery, A; Dakouo, M; Salami, H; Moustapha lo, M; Ba, A; Diop, M; Bezeid El Mamy, A; Salem El Arabi, A; Barry, Y; Isselmou, E; Habiboullah, H; Salem Lella, A; Doumbia, B; Baba gueye, M; Savadogo, J; Ouattara, L; Minougou, G; Libeau, G and Bataille, A (2019). Genetic evidence for transboundary circulation of peste des petits ruminants across West Africa. Front. Vet. Sci., 6: 1-7.
Truong, T; Boshra, H; Embury-Hyatt, C; Nfon, C; Gerdts, V; Tikoo, S; Babiuk, LA; Kara, P; Chetty, T; Mather, A; Wallace, DB and Babiuk, S (2014). Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection. PLoS One. 9: 1-13.
Woma, TY; Adombi, CM; Yu, D; Qasim, AMM; Sabi, AA; Maurice, NA; Maurice, OD; Loitsch, A; Bailey, D; Shamaki, D; Dundon, WG and Quan, M (2016). Co-circulation of peste-des-petits-ruminants virus Asian lineage IV with Lineage II in Nigeria. Transbound Emerg. Dis., 63: 235-242.
Zhou, XY; Wang, Y; Zhu, J; Miao, Q; Zhu, L; Zhan, L; Wang, G and Liu, G (2018). First report of peste des petits ruminants virus lineage II in Hydropotes inermis, China. Transbound. Emerg. Dis., 65: e205-e209. | ||
|
آمار تعداد مشاهده مقاله: 387 تعداد دریافت فایل اصل مقاله: 361 |
||