| تعداد نشریات | 24 |
| تعداد شمارهها | 881 |
| تعداد مقالات | 7,867 |
| تعداد مشاهده مقاله | 14,699,815 |
| تعداد دریافت فایل اصل مقاله | 12,550,021 |
Influence of topical NPH-insulin use on wound healing and oxidative stress in alkali corneal burns: an experimental study in a rabbit model | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 3 - شماره پیاپی 88، 2024، صفحه 202-209 اصل مقاله (478.98 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.47545.6867 | ||
| نویسندگان | ||
| B. Kurt* 1؛ L. Balyen2؛ O. Atakişi3؛ S. Yiğit4؛ U. Aydin1؛ İ. Özaydin1 | ||
| 1Department of Surgery, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey | ||
| 2Department of Ophthalmology, Faculty of Medicine, Harran University, Şanlıurfa, Turkey | ||
| 3Department of Chemistry, Faculty of Science and Letter, Kafkas University, Kars, Turkey | ||
| 4Department of Histology and Embryology, Faculty of Medicine, Kafkas University, Kars, Turkey | ||
| چکیده | ||
| Background: In acute alkali corneal burns, the routine treatment protocol includes suppressing inflammation using corticosteroids. In recent years, there has been a growing interest in the role of insulin in wound healing research due to its remarkable positive effects. Aims: The present study aimed to compare the healing effects of dexamethasone -the conventional approach- and NPH-insulin on alkali corneal burns. Methods: An alkali corneal burn was created using Ca (OH)2 in all subjects. One group (n=6) was treated with an ophthalmic ointment containing dexamethasone (group I), and another group (n=6) was treated with an insulin ointment (group II). The control group (n=6) received no treatment (group III). Clinical changes in the corneal burn areas were monitored on days 3, 7, 14, and 21. The animals were euthanized on day 21. The excised corneas were examined for histopathological, immunohistochemical, and biochemical changes. Results: A more successful clinical recovery graph was drawn for group II. Particularly, a significant (P<0.05) improvement was detected in group II on day 21. The highest positivity in MMP-9 corneal staining was found in group I. Group II had a significant (P<0.05) increase in total antioxidant capacity (TAC) values. The treatment groups showed a significant (P<0.05) decrease in total oxidant content (TOC) values and a significant (P<0.05) increase in reduced glutathione (GSH) levels as compared with the control group. Conclusion: Topical NPH-insulin provided rapid and uncomplicated clinical recovery of alkali corneal burns. Insulin and dexamethasone showed similar effects like increased antioxidant molecules and decreased oxidant substances which indicated that insulin may prevent free radical formation in the cornea. | ||
| کلیدواژهها | ||
| Corneal alkali burn؛ Insulin؛ Oxidative stress؛ Rabbit؛ Wound healing | ||
| مراجع | ||
|
Bacus, S; Flowers, JL; Press, MF; Bacus, JW and Mccarty, KS Jr (1988). The evaluation of estrogen receptor in primary breast carcinoma by computer-assisted image analysis. Am. J. Clin. Pathol., 90: 233-239.
Bashkaran, K; Zunaina, E; Bakiah, S; Sulaiman, SA; Sirajudeen, K and Naik, V (2011). Anti-inflammatory and antioxidant effects of Tualang honey in alkali injury on the eyes of rabbits: Experimental animal study. BMC Complement. Altern. Med., 11: 1-11.
Bayram, P; Billur, D; Kizil, S; Caliskan, H and Can, B (2022). Alterations in hippocampal neurogenesis and hippocampal insulin signaling pathway in rat with metabolic syndrome. Iran J. Basic Med. Sci., 25: 1308-1316.
Beutler, E; Duron, O and Kelly, BM (1963). Improved method for the determination of blood glutathione. J. Lab. Clin. Med., 61: 882-888.
Bizrah, M; Yusuf, A and Ahmad, S (2019). An update on chemical eye burns. Eye. 33: 1362-1377.
Bradford, MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
Bruze, M; Fregert, S and Gruvberger, B (2000). Chemical skin burns. In: Kanerva, L; Wahlberg, JE; Elsner, P and Maibach, HI (Eds.), Handbook of occupational dermatology. (1st Edn.), Berlin, Springer. PP: 325-332.
Cade, F; Paschalis, EI; Regatieri, CV; Vavvas, DG; Dana, R and Dohlman, CH (2014). Alkali burn to the eye: protection using TNF-a inhibition. Cornea. 33: 382-389.
Chen, X; Liu, Y and Zhang, X (2012). Topical insulin application improves healing by regulating the wound inflammatory response. Wound Repair Regen., 20: 425-434.
Chen, Y; Mehta, G and Vasiliou, V (2009). Antioxidant defenses in the ocular surface. Ocul. Surf., 7: 176-185.
Choe, HR; Yoon, CH and Kim, MK (2019). Ocular surface reconstruction using circumferentially - trephined autologous oral mucosal graft transplantation in limbal stem cell deficiency. Korean J. Ophthalmol., 33: 16-25.
Fish, R and Davidson, RS (2010). Management of ocular thermal and chemical injuries, including amniotic membrane therapy. Curr. Opin. Ophthalmol., 21: 317-321.
Fung, AT; Tran, T; Lim, LL; Samarawickrama, C; Arnold, J; Gillies, M; Catt, C; Mitchell, L; Symons, A; Buttery, R; Cottee, L; Tumuluri, K and Beaumont, P (2020). Local delivery of corticosteroids in clinical ophthalmology: A review. Clin. Exp. Ophthalmol., 48: 366-401.
Gakhramanov, FS (2005). Effect of natural antioxidants on antioxidant activity and lipid peroxidation in eye tissue of rabbits with chemical burns. Bull Exp. Biol. Med., 140: 289-291.
Gkountelia, A (2021). Periocular burns: A literature review of classification, management protocols and outcomes of treatment. Eye News. 28: 1-5.
Günay, C; Sagliyan, A; Yilmaz, S; Polat, E; Kaya, E; Han, MC and Karabulut, KK (2020). Investigation of the antioxidant effects of amniotic fluid on corneal alkali burns. FU. Vet. J. Health Sci., 34: 165-171.
Hakami, NY; Dusting, GJ; Chan, EC; Shah, MH and Peshavariya, HM (2020). Wound healing after alkali burn injury of the cornea involves Nox4-type NADPH oxidase. Invest. Ophthalmol. Vis. Sci., 61: 20.
Hrynyk, M and Neufeld, RJ (2014). Insulin and wound healing. Burns.40: 1433-1446.
Hughes, WF Jr (1946). Alkali burns of the eye. I. Review of the literature and summary of present knowledge. Arch. Ophthal., 35: 423-449.
Kato, T; Saika, S and Ohnishi, Y (2006). Effects of the matrix metalloproteinase inhibitor GM6001 on the destruction and alteration of epithelial basement membrane during the healing of post-alkali burn in rabbit cornea. Jpn. J. Ophthalmol., 50: 90-95.
Kesper, C; Heinzelmann, J; Viestenz, A; Hammer, T; Foja, S; Stein, M and Viestenz, A (2023). Allogeneic limbal transplants integrate into the corneal surface and lead to an improved visual acuity. J. Clin. Med., 12: 645.
Kim, DH; Im, ST; Yoon, JY; Kim, S; Kim, MK; Chung, MH and Park, CK (2021). Comparison of therapeutic effects between topical 8-oxo-2′-deoxyguanosine and corticosteroid in ocular alkali burn model. Nature. 11: 6909.
Kolesnikov, AV; Kirsanova, IV; Sokolova, AI and Ban, EM (2020). Oxidative stress and methods of its correction in corneal burns (review). Saratov J. Med. Sci. Res., 16: 459-463.
Lobmann, R; Ambrosch, A; Schultz, G; Waldmann, K; Schiweck, S and Lehnert, H (2002). Expression of matrix-metalloproteinases and their inhibitors in the wounds of diabetic and non-diabetic patients. Diabetologia. 45: 1011-1016.
Lorenzana-Blanco, N; Santander-Garcia, D; Güell, JL and Alejandre-Alba, N (2023). Acute management of ocular chemical burns: A review. J. EuCornea. 11: 1-11.
Makarov, PV; Titkova, SM; Anurov, MV; Mikhal’chik, EV; Chesnokova, NB; Beznos, OV; Stoliarova, EN; Oganesian, OG; Trofimova, MV; Akopian, AV; Kliuchikov, V and Korkina, LG (2005). Examination of the local antioxidative system of the eye in experimental corneal burn injury and the prospects for pharmacological correction of its parameters. Vestn. Oftalmol., 121: 40-43.
Moghadam, MR; Jafarinasab, MR; Yousefi, Z; Moghaddam, AS; Memarzadeh, H and Kanavi, MR (2020). Aloe vera gel-derived eye drops for alkaline corneal injury in a rabbit model. J. Ophthalmic Vis. Res., 15: 7-15.
Mohamed, ES and Ali, MA (2018). Autologus serum and sodium hyaluronate role in alkali corneal burn healing. J. Arab. Soc. Med. Res., 13: 39-44.
Mohan, R; Rinehart, WB; Bargagna-Mohan, P and Fini, ME (1998). Gelatinase B/lacZ transgenic mice, a model for mapping gelatinase B expression during developmental and injury-related tissue remodeling. J. Biol. Chem., 273: 25903-25914.
Ozaydin, I; Aksoy, O; Yayla, S; Kurt, B; Kilic, E; Bingol, SA; Can, I and Deprem, T (2018). Clinical, histopathological and immunohistochemical evaluation of the effects of topical NPH-insulin on full-thickness open wounds: An in vivo study in diabetic and non-diabetic mice. Ankara Univ. Vet. Fak. Derg., 65: 219-228.
Ozturk, F; Kurt, E; Cerci, M; Emiroglu, L; Inan, U; Turker, M and Ilker, S (2000). The effect of propolis extract in experimental chemical corneal injury. Ophthalmic Res., 32: 13-18.
Paschalis, EI; Zhou, C; Lei, F; Scott, N; Kapoulea, V; Robert, MC; Vavvas, D; Dana, R; Chodosh, J and Dohlman, CH (2017). Mechanisms of retinal damage after ocular alkali burns. Am. J. Pathol., 187: 1327-1342.
Pfister, RR and Pfister, DR (2005). Alkali injuries of the eye. In: Krachmer, JH; Mannis, MJ and Holland, EJ (Eds.), Cornea. (3rd Edn.), Cincinnati, Elsevier Mosby. PP: 1285-1293.
Poon, SHL; Wong, WHL; Bu, Y; Lo, ACY; Jhanji, V; Chan, YK and Shih, KC (2020). A systematic review of emerging therapeutic strategies in the management of chemical injuries of the ocular surface. Eye Contact Lens. 46: 329-340.
Ruban, VV; Anbukkarasi, M; Anand, T; Thomas, PA and Geraldine, P (2019). Oxidative stress in corneal tissue in experimental keratitis due to Aspergillus flavus: Effect of topical voriconazole therapy. Biocatal. Agric. Biotechnol., 21: 101323. Salman, IA; Kiziltunc, A and Baykal, O (2011). The effect of alkali burns on corneal glutathione peroxidase activities in rabbits. Turk J. Med. Sci., 41: 483-486.
Saroglu, M and Arikan, N (2002). Research on the comparison of various anticollagenase drugs in the treatment of experimentally induced corneal alkali burns in rabbits. J. Istanbul. Univ. Vet. Sci., 28: 287-300.
Schramm, DD; Karim, M; Schrader, HR; Holt, RR; Cardetti, M and Keen, CL (2003). Honey with high levels of antioxidants can provide protection to healthy human subjects. J. Agric. Food Chem., 51: 1732-1735.
Singh, A; Maurya, OP; Jagannadhan, MV and Patel, A (2012). Matrix metalloproteinases (MMP-2 and MMP-9) activity in corneal ulcer and ocular surface disorders determined by gelatin zymography. J. Ocul. Biol. Dis. Infor., 5: 31-35.
Soleimani, M and Naderan, M (2020). Management strategies of ocular chemical burns: current perspectives. Clin. Ophthalmol., 14: 2687-2699.
Wang, J and Xu, J (2020). Effects of topical insulin on wound healing: A review of animal and human evidence. Diabetes Metab. Syndr. Obes., 13: 719-727.
Williams, DL (2008). Oxidative stress and the eye. Vet. Clin. North. Am. Small Anim. Pract., 38: 179-192.
Yao, L; Li, ZR; Su, WR; Li, YP; Lin, ML; Zhang, WX; Liu, Y; Wan, Q and Liang, D (2012). Role of mesenchymal stem cells on cornea wound healing induced by acute alkali burn. PLoS One. 7: e30842.
Yuan, HP; Lu, SR and Wang, BL (1994). An experimental study of treatment with superoxide dismutase for alkali burn in the anterior segment of the rabbit eye. Chin. J. Ophthalmol., 30: 50-52. | ||
|
آمار تعداد مشاهده مقاله: 574 تعداد دریافت فایل اصل مقاله: 436 |
||