| تعداد نشریات | 24 |
| تعداد شمارهها | 874 |
| تعداد مقالات | 7,803 |
| تعداد مشاهده مقاله | 14,220,749 |
| تعداد دریافت فایل اصل مقاله | 12,165,053 |
Toxinotyping of Clostridium perfringens strains isolated from broiler flocks with necrotic enteritis and evaluation of the effect of toxins on Leghorn Male Hepatoma cells | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 2 - شماره پیاپی 87، 2024، صفحه 135-142 اصل مقاله (509.08 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.47200.6808 | ||
| نویسندگان | ||
| C. Katalani1؛ Gh. Ahmadian* 2؛ Gh. Nematzadeh1؛ J. Amani3؛ P. Ehsani4؛ J. Razmyar5؛ M. Shafiei6؛ E. Mirabzadeh Ardakani7؛ L. Ghazizadeh8 | ||
| 1Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agriculture Science and Natural Resource University (SANRU), Sari, Iran | ||
| 2Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran | ||
| 3Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran | ||
| 4Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran | ||
| 5Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 6Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran | ||
| 7Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran | ||
| 8MSc in Developmental Botany, National Cell Bank of IRAN, Pasteur Institute of Iran, Tehran, Iran | ||
| چکیده | ||
| Background: Necrotic enteritis (NE) is an economically important disease, caused by Clostridium perfringens type G strains, and is one of the major targets of antibiotics used in poultry feed. Aims: This study aimed to genotypically characterize virulent strains of C. perfringens isolated from healthy and diseased birds in Iran. Methods: Eleven isolates were derived from necrotic enteritis cases, and 27 were from healthy chickens. Isolations were performed using blood agar. To assess whether zmp is generally associated with avian NE, 38 C. perfringens isolates were screened using PCR and western blotting. The involvement of these toxins as virulence factors was investigated using cytotoxicity assays. Results: All isolates carried the phospholipase c (plc) gene regardless of their origin and virulence. The zinc metallopeptidase (zmp) gene was found in the isolates collected from birds affected by necrotic enteritis. Furthermore, Necrotic enteritis like B (NetB) was only found in 36.36% of the isolates derived from necrotic enteritis-infected birds. Western blot analysis further confirmed the expression of Alpha toxin, NetB, and Zmp in different isolates. Incubation of Leghorn Male Hepatoma (LMH) cells with crude C. perfringens toxins indicated that the supernatants of all bacterial strains were toxic toward LMH cells at different dilutions. In addition, crude toxins of the Cp28 strain expressing Alpha toxin, Zmp, and NetB showed an approximately 50% cytotoxic dose (CD50) at a 1:34 dilution. Strain Cp119.2, which produces both ZMP and the Alpha toxin, and strain Cp48, which only produces the Alpha toxin, showed CD50 at 1:23 and 1:4 dilutions, respectively. Conclusion: It seems that both NetB and Zmp play major roles in the cytotoxicity and pathogenicity of this organism. | ||
| کلیدواژهها | ||
| Clostridium perfringens؛ LMH cell line؛ Necrotic enteritis؛ NetB؛ Zinc metallopeptidase | ||
| مراجع | ||
|
Afshari, A; Jamshidi, A; Razmyar, J and Rad, M (2015). Genotyping of Clostridium perfringens isolated from broiler meat in northeastern of Iran. Vet. Res. Forum. 6: 279-284.
Caly, DL; D’Inca, R and Auclair Eand Drider, D (2015). Alternatives to antibiotics to prevent necrotic enteritis in broiler chickens: A microbiologist’s perspective. Front. Microbiol., 6: 1336-1348 https://doi.org/10.3389/fmicb. 2015.01336.
Cheung, JK; Keyburn, AL; Carter, GP; Lanckriet, AL; Van Immerseel, F; Moore, RJ and Rood, JI (2010). The VirSR two-component signal transduction system regulates NetB toxin production in Clostridium perfringens. Infect. Immun., 78: 3064-3072. https://doi.org/10.1128/IAI.00123-10.
Cooper, KK and Songer, JG (2009). Necrotic enteritis in chickens: a paradigm of enteric infection by Clostridium perfringens type A. Anaerobe. 15: 55-60. https://doi.org/ 10.1016/j.anaerobe.2009.01.006.
Katalani, C; Ahmadian, G; Nematzadeh, G; Amani, J; Ehsani, P; Razmyar, J and Kiani, G (2020a). Immunization with oral and parenteral subunit chimeric vaccine candidate confers protection against Necrotic Enteritis in chickens. Vaccine. 38: 7284-7291. https://doi.org/10.1016/j.vaccine.2020.09.047.
Katalani, C; Nematzadeh, G; Ahmadian, G; Amani, J; Kiani, G and Ehsani, P (2020b). In silico design and in vitro analysis of a recombinant trivalent fusion protein candidate vaccine targeting virulence factor of Clostridium perfringens. Int. J. Biol. Macromol., 146: 1015-1023. https://doi.org/10.1016/j.ijbiomac.2019.09.227.
Keyburn, AL; Boyce, JD; Vaz, P; Bannam, TL; Ford, ME; Parker, D; Di Rubbo, A; Rood, JI and Moore, RJ (2008). NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens. PLoS Pathog., 4: e26.
Keyburn AL; Portela RW; Sproat K; Ford ME; Bannam TL; Yan X; Rood JI and Moore RJ (2013). Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis. Vet Res., 44: 1-8. https://doi.org/10.1186/1297-9716-44-54.
Keyburn, AL; Yan, XX; Bannam, TL; Van Immerseel, F; Rood, JI and Moore, RJ (2010). Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin. Vet. Res., 41: 1-8.
Kiu, R and Hall, LJ (2018). An update on the human and animal enteric pathogen Clostridium perfringens. Emerg. Microbes. Infect., 7: 1-15. https://doi.org/10.1038/s41426-018-0144-8.
Kulkarni, RR; Parreira, VR; Sharif, S and Prescott, JF (2006). Clostridium perfringens antigens recognized by broiler chickens immune to necrotic enteritis. Clin. Vaccine Immunol., 13: 1358-1362. https://doi.org/10.1128/CVI. 00292-06.
Kulkarni, RR; Parreira, VR; Sharif, S and Prescott, JF (2008). Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens. Vaccine. 26: 4194-4203. https://doi.org/10.1016/j.vaccine. 2008.05.079.
Laemmli, UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685. https://doi.org/10.1038/227680a0.
Lanckriet, A; Timbermont, L; Eeckhaut, V; Haesebrouck, F; Ducatelle, R and Van Immerseel, F (2010). Variable protection after vaccination of broiler chickens against necrotic enteritis using supernatants of different Clostridium perfringens strains. Vaccine. 28: 5920-5923. https://doi.org/10.1016/j.vaccine.2010.06.035.
Lepp, D; Gong, J; Songer, JG; Boerlin, P; Parreira, VR and Prescott, JF (2013). Identification of accessory genome regions in poultry Clostridium perfringens isolates carrying the netB plasmid. J. Bacteriol., 195: 1152-1166. https://doi.org/10.1128/JB.01032-12.
Nakjang, S; Ndeh, DA; Wipat, A; Bolam, DN and Hirt, RP (2012). A novel extracellular metallopeptidase domain shared by animal host-associated mutualistic and pathogenic microbes. PLoS One. 7: e30287. https://doi.org/ 10.1371/journal.pone.0030287.
Noach, I; Ficko-Blean, E; Pluvinage, B; Stuart, C; Jenkins, ML; Brochu, D; Buenbrazo, N; Wakarchuk, W; Burke, JE and Gilbert, M (2017). Recognition of protein-linked glycans as a determinant of peptidase activity. PNAS. 114: E679-E688.
Pluvinage, B; Ficko-Blean, E; Noach, I; Stuart, C; Thompson, N; McClure, H; Buenbrazo, N; Wakarchuk, W and Boraston, AB (2021). Architecturally complex O-glycopeptidases are customized for mucin recognition and hydrolysis. PNAS. 118: e2019220118. https://doi.org/10. 1073/pnas.2019220118.
Prescott, JF; Parreira, VR; Mehdizadeh Gohari, I; Lepp, D and Gong, J (2016). The pathogenesis of necrotic enteritis in chickens: what we know and what we need to know: a review. Avian Pathol., 45: 288-294. https://doi.org/10. 1080/03079457.2016.1139688.
Razmyar, J; Kalidari, GA; Tolooe, A; Rad, M and Movassaghi, AR (2014). Genotyping of Clostridium perfringens isolated from healthy and diseased ostriches (Struthio camelus). Iran. J. Microbiol., 6: 31-36.
Razmyar, J; Peighambari, SM and Zamani, AH (2017). Detection of a newly described bacteriocin, perfrin, among C lostridium perfringens isolates from healthy and diseased ostriches and broiler chickens in Iran. Avian Dis., 61: 387-390.
Rood, JI; Adams, V; Lacey, J; Lyras, D; McClane, BA; Melville, SB; Moore, RJ; Popoff, MR; Sarker, MR; Songer, JG; Uzal, FA and Van Immerseel, F (2018). Expansion of the Clostridium perfringens toxin-based typing scheme. Anaerobe. 53: 5-10. https://doi.org/10.1016/ j.anaerobe.2018.04.011.
Shimizu, T; Ohtani, K; Hirakawa, H; Ohshima, K; Yamashita, A; Shiba, T; Ogasawara, N; Hattori, M; Kuhara, S and Hayashi, H (2002). Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. Proc. Natl. Acad. Sci., 99: 996-1001. https://doi.org/ 10.1073/pnas.022493799.
Shojadoost, B; Vince, AR and Prescott, JF (2012). The successful experimental induction of necrotic enteritis in chickens by Clostridium perfringens: a critical review. Vet. Res., 43: 1-12. https://doi.org/10.1186/1297-9716-43-74.
Van Immerseel, F; Rood, JI; Moore, RJ and Titball, RW (2009). Rethinking our understanding of the pathogenesis of necrotic enteritis in chickens. Trends Microbiol., 17: 32-36.
Wade, B and Keyburn, A (2015). The true cost of necrotic enteritis. World Poult., 31: 16-17.
Wade, B; Keyburn, AL; Haring, V; Ford, M; Rood, JI and Moore, RJ (2020). Two putative zinc metalloproteases contribute to the virulence of Clostridium perfringens strains that cause avian necrotic enteritis. J. Vet. Diagn. Invest., 32: 259-267.
Wilde, S; Jiang, Y; Tafoya, AM; Horsman, J; Yousif, M; Vazquez, LA and Roland, KL (2019). Salmonella-vectored vaccine delivering three Clostridium perfringens antigens protects poultry against necrotic enteritis. PLoS One. 14: e0197721. https://doi.org/10.1371/journal.pone. 0197721. Yu, Q; Lepp, D; Gohari, IM; Wu, T; Zhou, H; Yin, X; Yu, H; Prescott, JF; Nie, SP and Xie, MY (2017). The Agr-like quorum sensing system is required for pathogenesis of necrotic enteritis caused by Clostridium perfringens in poultry. Infect. Immun., 85: e00975-16.
Yuan, B; Sun, Z; Lu, M; Lillehoj, H; Lee, Y; Liu, L; Yan, X; Yang, DA and Li, C (2022). Immunization with pooled antigens for Clostridium perfringens conferred partial protection against experimental necrotic enteritis in broiler chickens. Vaccines (Basel). 10: 979-990. https://doi.org/ 10.3390/vaccines10060979.
Zhou, H; Lepp, D; Pei, Y; Liu, M; Yin, X; Ma, R; Prescott, JF and Gong, J (2017). Influence of pCP1NetB ancillary genes on the virulence of Clostridium perfringens poultry necrotic enteritis strain CP1. Gut Pathog., 9: 1-7. https://doi.org/10.1186/s13099-016-0152-y. | ||
|
آمار تعداد مشاهده مقاله: 405 تعداد دریافت فایل اصل مقاله: 384 |
||