اخبار و اعلانات

 آمار

تعداد نشریات24
تعداد شماره‌ها859
تعداد مقالات7,646
تعداد مشاهده مقاله13,578,484
تعداد دریافت فایل اصل مقاله11,684,902
Hosseini, Saman, Sharifi, Rouhallah, Habibi, Alireza, Beheshti Ale-Agha, Ali. (1403). Isolation and characterization of thermotolerant hydrocarbon degrading bacteria which sustained the activity at extreme salinity and high osmotic conditions. , 14(1), 37-46. doi: 10.22099/mbrc.2024.49747.1946
Saman Hosseini; Rouhallah Sharifi; Alireza Habibi; Ali Beheshti Ale-Agha. "Isolation and characterization of thermotolerant hydrocarbon degrading bacteria which sustained the activity at extreme salinity and high osmotic conditions". , 14, 1, 1403, 37-46. doi: 10.22099/mbrc.2024.49747.1946
Hosseini, Saman, Sharifi, Rouhallah, Habibi, Alireza, Beheshti Ale-Agha, Ali. (1403). 'Isolation and characterization of thermotolerant hydrocarbon degrading bacteria which sustained the activity at extreme salinity and high osmotic conditions', , 14(1), pp. 37-46. doi: 10.22099/mbrc.2024.49747.1946
Hosseini, Saman, Sharifi, Rouhallah, Habibi, Alireza, Beheshti Ale-Agha, Ali. Isolation and characterization of thermotolerant hydrocarbon degrading bacteria which sustained the activity at extreme salinity and high osmotic conditions. , 1403; 14(1): 37-46. doi: 10.22099/mbrc.2024.49747.1946

Isolation and characterization of thermotolerant hydrocarbon degrading bacteria which sustained the activity at extreme salinity and high osmotic conditions

Molecular Biology Research Communications
دوره 14، شماره 1، 2025، صفحه 37-46    اصل مقاله (282.83 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2024.49747.1946
نویسندگان
Saman Hosseini* 1؛ Rouhallah Sharifi1؛ Alireza Habibi2؛ Ali Beheshti Ale-Agha3
1Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
2Faculty of Petroleum and Chemical Engineering, Razi University, Kermanshah, Iran
3Department of Soil Science, College of Agriculture, Razi University, Kermanshah, Iran
چکیده
The bioremediation method is considered an economical and environmentally friendly strategy for the remediation of oil-contaminated soils. However, some oil field areas have extreme environmental conditions that make it difficult to establish microbes for bioreme-diation. In this study, bacteria were isolated from oil-contaminated soils of the Dehloran oil fields, which have very harsh soil and weather conditions. Soil samples were collected from two highly contaminated mud pits. The petroleum content and physicochemical characteristics of the soil were investigated. Soil samples pollution were about 8%, sandy and alkaline, and their EC reached up to 125.6 ds/m in some samples. The isolated bacteria were screened according to their ability to grow on the M9 mineral medium containing crude oil as the sole carbon source. Moreover, their physiological characteristics in diesel degradation were investigated. The phenotypic, biochemical, and molecular characteristics of selected isolates and their stability under extreme conditions such as drought, salinity and high temperatures were investigated. Two isolates NC39 and NB391 showed the highest ability in diesel degradation. The results of 16SrRNA sequencing showed that NC39 isolate had 98% similarity to Pseudomonas sp. and isolate NB391 belonged to Pantoea agglomerans with 99% similarity. These two isolates showed a high ability to tolerate high salinity (10%), temperature (50°C), and drought (-0.73 MPa) stress. Exploiting these extremophile strains is a promising tool in the bioremediation of oil-contaminated soils in extreme environments.
کلیدواژه‌ها
Bioremediation؛ Indigenous bacteria؛ Abiotic stress؛ Extreme environment؛ PCR
مراجع
  1. Hylemon PB, Cacciapuoti AF, White BA, Whitehead TR, Fricke RJ. 7 α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species VPI 12708. Oxford University Press. 1980.
  2. Schafer AN, Snape I, Siciliano SD. Influence of liquid water and soil temperature on petroleum hydrocarbon toxicity in Antarctic soil. Environ Toxicol Chem 2009;28:1409-1415.
  3. Kästner M. Degradation of aromatic and polyaromatic compounds. Biotechnol Set. 2001;211-239.
  4. Varjani SJ. Microbial degradation of petroleum hydrocarbons. Bioresour Technol 2017; 223:277-286.
  5. Varjani SJ, Upasani VN. Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514. Bioresour Technol 2016;222:195-201.
  6. Ebrahimi M, Fallah A, Sarikhani MR. Isolation and identification of oil-degrading bacteria from oil-polluted soils and assessment of their growth in the presence of gas oil. Water and Soil Sci 2013;23:109-121.
  7. Prince RC, Amande TJ, McGenity TJ. Prokaryotic hydrocarbon degraders. Taxonomy, Genomics ecophysiology of hydrocarbon-degrading microbes. 2019;1-39.
  8. McFarlin KM, Prince RC. Oil Contaminated Soil. Understanding Biodegradation and Remediation Strategies. Petrodiesel Fuels O. Konur, Editor., CRC Press 2021;25:911-935.
  9. Kumari B, Singh G, Sinam G, Singh DP. Microbial remediation of crude oil-contaminated sites. Environmental Concerns Sustainable Development. Springer Singapore 2020;333-351.
  10. Kuntz J, Nassr-Amellal N, Lollier M, Schmidt JE, Lebeau T. Effect of sludges on bacteria in agricultural soil. Analysis at laboratory and outdoor lysimeter scale. Ecotoxicol Environ Saf 2008;69:277-288.
  11. Radwan SS, Sorkhoh NA, Fardoun F, Al-Hasan RH. Soil management enhancing hydrocarbon biodegradation in the polluted Kuwaiti desert. Appl Microbiol Biotechnol 1995;44(1-2):265-270.
  12. Liu H, Gao H, Wu M, Ma C, Wu J, Ye X. Distribution characteristics of bacterial communities and hydrocarbon degradation dynamics during the remediation of petroleum-contaminated soil by enhancing moisture content. Microb Ecol 2020;80:202-211.
  13. Lozupone CA, Knight R. Global patterns in bacterial diversity. Proc Natl Acad Sci U S A 2007;104:11436-11440.
  14. Zhao B, Wang H, Mao X, Li R. Biodegradation of phenanthrene by a halophilic bacterial consortium under aerobic conditions. Curr Microbiol 2009;58:205-210.
  15. Naseri S, Beheshti Ale Agha A, Sharifi R, Bahraminejad S. Rhizobacteria modify soil biological indices and induce tolerance to osmotic stress in tomato depending on the salinity level and bacteria species. Braz J Microbiol 2022;53:1473-1481.
  16. Schaad NW, Jones JB, Chun W. Laboratory guide for the identification of plant pathogenic bacteria. Thrid Edition. Minnesota, USA: American Phytopathological Society, St Paul. 2001
  17. Cappello S, Santisi S, Calogero R, Hassanshahian M, Yakimov MM. Characterisation of oil-degrading bacteria isolated from bilge water. Water Air Soil Pollut 2012;223:3219-3226.
  18. Van der Weele CM, Spollen WG, Sharp RE, Baskin TI. Growth of Arabidopsis thaliana seedlings under water deficit studied by control of water potential in nutrient‐agar media. J Exp Bot 2000;51:1555-1562.
  19. Michel BE, Kaufmann MR. The osmotic potential of polyethylene glycol 6000. Plant Physiol 1973;51:914-916.
  20. Bulgari R, Trivellini A, Ferrante A. Effects of two doses of organic extract-based biostimulant on greenhouse lettuce grown under increasing NaCl concentrations. Front Plant Sci 2019;9:1870.
  21. Hosseini S, Marefat A. Characterization and genetic diversity of Xanthomonas translucens, the causal agent of bacterial stripe of wheat in Kermanshah province, Iran. Plant Prot 2021; 44:89-105.
  22. Jiang H, Dong H, Zhang G, Yu B, Chapman LR, Fields MW. Microbial diversity in water and sediment of Lake Chaka, an athalassohaline Lake in northwestern China. Appl Environ Microbiol 2006;72:3832-3845.
  23. Abou Khalil C, Prince VL, Prince RC, Greer CW, Lee K, Zhang B, Boufadel MC. Occurrence and biodegradation of hydrocarbons at high salinities. Sci Total Environ 2021; 762:143165.
  24. Kahlon RS. Biodegradation and bioremediation of organic chemical pollutants by Pseudomonas. in Pseudomonas: Molecular and applied biology, R.S. Kahlon, Editor., Springer: Switzerland 2016; 343-417.
  25. Niazy Z, Hassanshahian M, Ataei A. Isolation and characterization of diesel-degrading Pseudomonas strains from diesel-contaminated soils in Iran (Fars province). Pollut 2016; 2:67-75.
  26. Hong JH, Kim J, Choi OK, Cho KS, Ryu HW. Characterization of a diesel-degrading bacterium, Pseudomonas aeruginosa IU5, isolated from oil-contaminated soil in Korea. World J Microbiol Biotechnol 2005;21:381-384.
  27. Shukor MY, Hassan NAA, Jusoh AZ, Perumal N, Shamaan NA, MacCormack WP, Syed MA. Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica. J Environ Biol 2009;30:1-6.
  28. Dutkiewicz J, Mackiewicz B, Lemieszek M K, Golec M, Milanowski J. Pantoea agglomerans: a mysterious bacterium of evil and good. Part IV. Beneficial effects. Ann Agric Environ Med 2016;23:206-222.
  29. Afzal M, Yousaf S, Reichenauer TG, Sessitsch A. The inoculation method affects colonization and performance of bacterial inoculant strains in the phytoremediation of soil contaminated with diesel oil. Int J Phytoremediation 2012;14:35-47.
  30. Arslan M, Afzal M, Amin I, Iqbal S, Khan QM. Nutrients can enhance the abundance and expression of alkane hydroxylase CYP153 gene in the rhizosphere of ryegrass planted in hydrocarbon-polluted soil. PLoS One 2014;9:e111208.
  31. Tara N, Afzal M, Ansari TM, Tahseen R, Iqbal S, Khan QM. Combined use of alkane-degrading and plant growth-promoting bacteria enhanced phytoremediation of diesel contaminated soil. Int J Phytoremediation 2014;16:1268-1277.
  32. Zhou E, Crawford RL. Effects of oxygen, nitrogen, and temperature on gasoline biodegradation in soil. Biodegradation 1995;6:127-140.
  33. Teng Y, Luo Y, Ping L, Zou D, Li Z, Christie P. Effects of soil amendment with different carbon sources and other factors on the bioremediation of an aged PAH-contaminated soil. Biodegradation 2010;21:167-178.
  34. Das N, Chandran P. Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnol Res Int 2011;2011:941810.
  35. McFarlin KM, Prince RC, Perkins R, Leigh MB. Biodegradation of dispersed oil in arctic seawater at-1 C. PLoS One 2014;9:e84297.
  36. McWatters RS, Wilkins D, Spedding T, Hince G, Raymond B, Lagerewskij G, Terry D, Wise L, Snape I. On site remediation of a fuel spill and soil reuse in Antarctica. Sci Total Environ 2016;571:963-973.
آمار
تعداد مشاهده مقاله: 598
تعداد دریافت فایل اصل مقاله: 346


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب