| تعداد نشریات | 24 |
| تعداد شمارهها | 912 |
| تعداد مقالات | 8,158 |
| تعداد مشاهده مقاله | 15,838,936 |
| تعداد دریافت فایل اصل مقاله | 13,333,887 |
Distribution of maedi-visna virus in different organs of the spontaneously affected small ruminants in India | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 1 - شماره پیاپی 86، 2024، صفحه 41-47 اصل مقاله (1.02 M) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.47429.6844 | ||
| نویسندگان | ||
| Sh. Valecha1؛ H. S. Yadav2؛ N. Roopa1؛ S. D. Vinaykumar2؛ M. Bhardwaj3؛ V. Singh4؛ P. Kumar* 4 | ||
| 1MVSc Student in Veterinary Pathology, Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh-243122, India | ||
| 2Ph.D. Student in Veterinary Pathology, Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh-243122, India | ||
| 3Department of Veterinary Microbiology, College of Veterinary Science, CSK Himachal Pradesh Agriculture University, Palampur, Himachal Pradesh-176062, India | ||
| 4Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh-243122, India | ||
| چکیده | ||
| Background: Maedi-visna (MV) is a small ruminant lentiviral (SRLV) disease affecting sheep and goats, and causes pathological alterations in various organs including lungs, pulmonary lymph nodes, mammary glands, joints, and CNS. Aims: Present study was focused to detect the MV virus (MVV) nucleic acid and MVV p28 antigen in different organs of the spontaneously MVV affected sheep and goats. Methods: Total of 657 samples were collected from sheep and goats (169 blood, 136 lungs, 96 pulmonary lymph nodes, 74 brain, 54 mammary gland, 78 joints, and 50 spleen) and screened for MVV nucleic acid using nested PCR assay. Serum samples were screened for SRLV antibodies by cELISA. Immunolocalization of MVV was demonstrated by using the polyclonal antibody against p28 antigen by immunohistochemistry in lungs, lymph nodes, mammary glands, and joint tissues. Results: Out of 657 samples, 10.7% (70) were found positive for MVV. Among different organs, lungs showed highest positivity (25.7%) followed by mammary glands (14.8%), blood (9.5%), joint tissues (7.7%), brain (5.4%), and pulmonary lymph node (1.0%). SRLV antibodies were detected in 29.2% of the serum samples of both sheep and goats by cELISA. MVV p28 antigen immunostaining was observed in lungs, lymph nodes, mammary glands, and joint tissues. However, the presence of MVV p28 antigen could not be demonstrated in the brain tissues. Conclusion: The highest positivity of MVV in lung tissues indicated higher predilection of the virus in the pulmonary tissue. | ||
| کلیدواژهها | ||
| Immunohistochemistry؛ Maedi-visna؛ Seroprevalence؛ Small ruminants | ||
| مراجع | ||
|
Barquero, N; Arjona, A; Domenech, A; Toural, C; de Las Heras, A; Fernández-Garayzabal, JF; Ruiz-Santa Quiteria, JA and Gomez-Lucia, E (2011). Diagnostic performance of PCR and ELISA on blood and milk samples and serological survey for small ruminant lentiviruses in central Spain. Vet. Rec., 168: 20. https://doi.org/10.1136/vr.c4951.
Benavides, J; García-Pariente, C; Gelmetti, D; Fuertes, M; Ferreras, MC; García-Marín, JF and Pérez, V (2006). Effects of fixative type and fixation time on the detection of Maedi Visna virus by PCR and immunohistochemistry in paraffin-embedded ovine lung samples. J. Virol. Meth., 137: 317-324. https://doi.org/10.1016/j.jviromet.2006.07. 009.
Blacklaws, BA (2012). Small ruminant lentiviruses: immunopathogenesis of visna-maedi and caprine arthritis and encephalitis virus. Comp. Immunol. Microbiol. Infect. Dis., 35: 259-269. https://doi.org/10.1016/j.cimid.2011.12. 003.
Carrozza, ML; Mazzei, M; Bandecchi, P; Arispici, M and Tolari, F (2003). In situ PCR-associated immuno-histochemistry identifies cell types harbouring the Maedi-Visna virus genome in tissue sections of sheep infected naturally. J. Virologic. Meth., 107: 121-127. https://doi.org/ 10.1016/S0166-0934(02)00208-2.
Colitti, B; Coradduzza, E; Puggioni, G; Capucchio, MT; Reina, R; Bertolitti L and Rosati S (2019). A new approach for small ruminant lentivirus full genome characterization revealed the circulation of divergent strains. PLoS One. 14(2): e0212585. https://doi.org/10. 1371/journal.pone.0212585.
Dar, JA (2017). Monitoring and pathology of viral pneumonias with special reference to retroviral infections in sheep and goats. MVSc Thesis, ICAR-IVRI Deemed University, Izatnagar, Bareilly, India.
De Andres, D; Klein, D and Watt, NJ (2005). Diagnostic tests for small ruminant lentiviruses. Vet. Microbiol., 107: 49-62. https://doi.org/10.1016/j.vetmic.2005.01.012.
Gayo, E; Cuteri, V; Polledo, L; Rossi, G; GarciaMarin, J and Preziuso, S (2018). Genetic characterization and phylogenetic analysis of small ruminant lentiviruses detected in spanish Assaf sheep with different mammary lesions. Virus. 10(6): 315. https://doi.org/10.3390/ v10060315.
Gomez-Lucia, E; Barquero, N and Domenech, A (2018). Maedi-Visna: current perspectives. Vet. Med. Res. Rep., 9: 11. https://doi.org/10.2147/VMRR.S136705.
Herrmann-Hoesing, LM; Noh, SM; Snekvik, KR; White, SN; Schneider, DA; Truscott, T and Knowles, DP (2010). Ovine progressive pneumonia virus capsid antigen as found in CD163-and CD172a-positive alveolar macrophages of persistently infected sheep. Vet. Path., 47: 518-528. https://doi.org/10.1177/0300985809359605.
International Committee on Taxonomy of Viruses (2022). Current ICTV taxonomy release. https://ictv.global/ taxonomy.
Kumar, K; Kumar, P; Sindhoora, K; Valecha, S; Kumar, R; Singh, V and Singh, R (2022). Detection and immune cell response of natural maedi visna virus (MVV) infection in Indian sheep and goats. Microb. Pathog., 165: 105467. https://doi.org/10.1016/j.micpath.2022.105467.
Larruskain, A and Jugo, BM (2013). Retroviral infections in sheep and goats: Small ruminant lentiviruses and host interaction. Viruses. 5: 2043-2061. https://doi.org/10.3390/ v5082043.
Lujan, L; García, JM; de Luco Fernández, D; Vargas, A and Badiola, JJ (1991). Pathological changes in the lungs and mammary glands of sheep and their relationship with maedi-visna infection. Vet. Rec., 129: 51-54. https://doi. org/10.1136/vr.129.3.51.
Mishra, S; Kumar, P; Dar, JA; George, N; Singh, R; Singh, V and Singh, R (2020). Detection and prevalence of small ruminant lentiviral (SRLV) infections in Indian sheep and goats. Indian J. Vet. Path., 44: 207-211. https://doi.org/10. 5958/0973-970X.2020.00043.7.
Peterhans, E; Greenland, T; Badiola, J; Harkiss, G; Bertoni, G; Amorena, B; Eliaszewicz, M; Juste, R; Krassnig, R; Lafont, JP and Lenihan, P (2004). Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes. Vet. Res., 35: 257-274. Pinczowski, P; Sanjose, L; Gimeno, M; Crespo, H; Glaria, I and Amorena, B (2017). Small ruminant lentiviruses in sheep: Pathology and tropism of 2 strains using the bone marrow route. Vet. Pathol., 54: 413-424. https://doi.org/10. 1177/0300985816688742.
Preziuso, S; Magi, GE; Mari, S and Renzoni, G (2013). Detection of visna maedi virus in mesenteric lymph nodes and in other lymphoid tissues of sheep three years after respiratory infection. Vet. Med., 58: 359-363.
Preziuso, S; Taccini, E; Rossi, G; Renzoni, G and Braca, G (2003). Experimental maedi visna virus infection in sheep: a morphological, immunohistochemical and PCR study after three years of infection. Euro. J. Histo. Chem., 47: 373-378. https://doi.org/10.4081/849.
Sigurdsson, B and Palsson, PA (1954). Visna of sheep. A slow demyelinating infection. Brit. Exp. Pathol., 39: 519-528.
Valecha, S; Roopa, N; Yadav, HS; Singh, R; Singh, V and Kumar, P (2023). Co-infection of maedi visna virus (MVV) with jaagsiekte sheep retrovirus (JSRV) and mycoplasma in Indian sheep and goats. Indian J. Vet. Pathol., 47: 13-17.
Vaught, JB and Henderson, MK (2011). Biological sample collection, processing, storage and information management. IARC Sci. Publ., 163: 23-42.
Yadav, HS; Neha; Vinay Kumar, SD; Singh, CP; Jana, C; Singh, V and Kumar, P (2024a). Mixed infection of ovine pulmonary adenocarcinoma and maedi in sheep - A case study. Indian J. Vet. Pathol., 48: 196-198.
Yadav, HS; Neha; Vinay Kumar, SD; Singh, V and Kumar, P (2024b). Detection of maedi visna virus (MVV) in milk samples of Indian goats. Internat. J. Adv. Biochem. Res., SP-8(1): 143-145. | ||
|
آمار تعداد مشاهده مقاله: 614 تعداد دریافت فایل اصل مقاله: 453 |
||