| تعداد نشریات | 24 |
| تعداد شمارهها | 859 |
| تعداد مقالات | 7,646 |
| تعداد مشاهده مقاله | 13,577,941 |
| تعداد دریافت فایل اصل مقاله | 11,684,538 |
First report of detection of mcr-1 and virulence genes in avian pathogenic Escherichia coli in the center of Algeria | ||
| Iranian Journal of Veterinary Research | ||
| دوره 25، شماره 1 - شماره پیاپی 86، 2024، صفحه 8-15 اصل مقاله (283.66 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2024.47413.6840 | ||
| نویسندگان | ||
| Z. Halfaoui* 1، 2؛ H. Rahab3؛ R. Achek4، 5؛ M. N. Menoueri6 | ||
| 1Laboratory of Biotechnologies Related to Animal Reproduction (LBRA), Blida-1 University, Blida (09000), Algeria | ||
| 2Institute of Veterinary Sciences, Blida-1 University, BP 270, Blida (09000), Algeria | ||
| 3Biotechnology Research Center, C.R.Bt, Ali Mendjli, BP E73, Constantine, Algeria | ||
| 4Department of Biology, Faculty of Nature and Life and Earth Sciences, Djilali Bounaama University, Khemis-Miliana 44225, Algeria | ||
| 5Laboratory of Food Hygiene and Quality Assurance System HASAQ, High National Veterinary School Oued Smar, Algiers, Algeria | ||
| 6Institute of Veterinary Sciences, Blida-1 University, BP 270, Blida (09000), Algeria; | ||
| چکیده | ||
| Background: Antimicrobial resistance in avian pathogenic Escherichia coli (APEC) represents a major concern in the avian industry worldwide and limited studies have investigated Colistin resistance among APEC in Algeria. Aims: Investigate antibiotic resistance, in particular, Colistin, and mediated-Colistin resistance (mcr) genes, as well as the virulence genes in APEC. Methods: One hundred E. coli were isolated from poultry suspected of colibacillosis. Antimicrobial susceptibility testing was done on 14 antibiotics by the disk diffusion method. Colistin minimum inhibitory concentration (MIC) was assessed by the broth microdilution method. Using multiplex PCR, mcr genes (mcr-1 to 5) and 7 virulence-related genes were investigated in Colistin-resistant isolates. Results: Results showed high resistance to Tetracycline (99%), Nalidixic acid (92%), Doxycycline (90%), Ampicillin (89%), Ofloxacin (74%), Sulfamethoxazole-Trimethoprim (72%), and Amoxicillin-Clavulanic acid (57%); in addition, 92% of isolates were multidrug resistant. The rate of resistance to Colistin was 27% (27/100) of which 96.3% (26/27) of isolates carried the mcr-1 gene. Twenty-five of the Colistin-resistant isolates (92.59%) had at least three virulence genes. The most frequently isolated virulence genes were: fimH (96.3%) followed by hlyF, iroN, and iss (77.7%, each), iutA and ompT were found in 59.25% and 55.5% of isolates, respectively. The most prevalent combination of virulence factors was hlyF-iss-iroN-iutA-ompT-fimH. Conclusion: This is the first report which highlighted Colistin resistance with the detection of mcr-1 in APEC isolates in the area of study. Colistin resistance and carriage of mcr-1 in virulent and multidrug-resistant isolates of E. coli are alarming and a surveillance program to limit the spread of these pathogens is mandatory. | ||
| کلیدواژهها | ||
| Antimicrobial resistance؛ Avian pathogenic Escherichia coli؛ Colistin resistance؛ Virulence genes | ||
| مراجع | ||
|
Ahmed, AM; Shimamoto, T and Shimamoto, T (2013). Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. Int. J. Med. Microbiol., 303: 475-483.
Al-Kandari, F and Woodward, MJ (2019). Genotypic and phenotypic diversity differences of presumptive commensal and avian pathogenic E. coli. Br. Poult. Sci., 60: 79-86.
Al-Mir, H; Osman, M; Drapeau, A; Hamze, M; Madec, JY and Haenni, M (2021). WGS analysis of clonal and plasmidic epidemiology of colistin-resistance mediated by mcr genes in the poultry sector in Lebanon. Front. Microbiol., 12: 1-10.
Amin, MB; Sraboni, AS; Hossain, MI; Roy, S; Mozmader, TAU; Unicomb, L; Rousham, EK and Islam, MA (2020). Occurrence and genetic characteristics of mcr-1-positive colistin-resistant E. coli from poultry environments in Bangladesh. J. Glob. Antimicrob. Resist., 22: 546-552.
Azam, M; Ehsan, I; Sajjad-ur-Rahman; Saleemi, MK; Javed, MR and Mohsin, M (2017). Detection of the colistin resistance gene mcr-1 in avian pathogenic Escherichia coli in Pakistan. J. Glob. Antimicrob. Resist., 11: 152-153.
Badr, H; Samir, A; El-Tokhi, EI; Shahein, MA; Rady, FM; Hakim, AS; Fouad, EA; El-Sady, EF and Ali, SF (2022). Phenotypic and genotypic screening of colistin resistance associated with emerging pathogenic Escherichia coli isolated from poultry. Vet. Sci., 9: 1-10.
Barbieri, NL; Nielsen, DW; Wannemuehler, Y; Cavender, T; Hussein, A; Yan, SG; Nolan, LK and Logue, CM (2017). Mcr-1 identified in avian pathogenic Escherichia coli (APEC). PLoS One. 12: 1-13.
Berrazeg, M; Hadjadj, L; Ayad, A; Drissi, M and Rolain, JM (2016). First detected human case in Algeria of mcr-1 plasmid-mediated colistin resistance in a 2011 Escherichia coli isolate. Antimicrob. Agents Chemother., 60: 6996-6997.
Blanco, JE; Blanco, M; Mora, A and Blanco, J (1997). Prevalence of bacterial resistance to quinolones and other antimicrobials among avian Escherichia coli strains isolated from septicemic and healthy chickens in Spain. J. Clin. Microbiol., 35: 2184-2185.
Chaalal, N; Touati, A; Yahiaoui-Martinez, A; Aissa, M; Sotto, A; Lavigne, J and Pantel, A (2021). Colistin-resistant Enterobacterales isolated from chicken meat in Western Algeria. Microb. Drug Resist., 27: 991-1002.
Chabou, S; Leangapichart, T; Okdah, L; Le Page, S; Hadjadj, L and Rolain, JM (2016). Real-time quantitative PCR assay with Taqman® probe for rapid detection of MCR-1 plasmid-mediated colistin resistance. New Microbes New Infect., 13: 71-74.
Chabou, S; Leulmi, H and Rolain, JM (2019). Emergence of mcr-1-mediated colistin resistance in Escherichia coli isolates from poultry in Algeria. J. Glob. Antimicrob. Resist., 16: 115-116.
Chelaghma, W; Loucif, L; Bendjama, E; Cherak, Z; Bendahou, M and Rolain, JM (2022). Occurrence of extended spectrum cephalosporin-, carbapenem- and colistin-resistant gram-negative bacteria in fresh vegetables, an increasing human health concern in Algeria. Antibiotics. 11: 1-16.
CLSI (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard, 9th Edn., CLSI document M07-A9.
CLSI (2018). M100 performance standards for antimicrobial susceptibility testing. In Clinical Laboratory Standards Institute. 28th Edn.
Cordoni, G; Woodward, MJ; Wu, H; Alanazi, M; Wallis, T and La Ragione, RM (2016). Comparative genomics of European avian pathogenic E. coli (APEC). BMC Genomics. 17: 1-21.
Dandachi, I; Chabou, S; Daoud, Z and Rolain, JM (2018). Prevalence and emergence of extended-spectrum cephalosporin-, carbapenem- and colistin-resistant gram negative bacteria of animal origin in the Mediterranean basin. Front. Microbiol., 9: 1-26.
Delicato, ER; de Brito, BG; Gaziri, LC and Vidotto, MC (2003). Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis. Vet. Microbiol., 94: 97-103.
De Oliveira, AL; Rocha, DA; Finkler, F; De Moraes, LB; Barbieri, NL; Pavanelo, DB; Winkler, C; Grassotti, TT; De Brito, KCT; De Brito, BG and Horn, F (2015). Prevalence of ColV plasmid-linked genes and in vivo pathogenicity of avian strains of Escherichia coli. Foodborne Pathog. Dis., 12: 679-685.
Dhaouadi, S; Soufi, L; Hamza, A; Fedida, D; Zied, C; Awadhi, E; Mtibaa, M; Hassen, B; Cherif, A; Torres, C; Abbassi, MS and Landolsi, RB (2020). Co-occurrence of mcr-1 mediated colistin resistance and β-lactamase-encoding genes in multidrug-resistant Escherichia coli from broiler chickens with colibacillosis in Tunisia. J. Glob. Antimicrob. Resist., 22: 538-545.
Dho-Moulin, M and Fairbrother, JM (1999). Avian pathogenic Escherichia coli (APEC). Vet. Res., 30: 299-316.
Dilhari, A; Sampath, A; Gunasekara, C; Fernando, N; Weerasekara, D; Sissons, C; McBain, A and Weerasekera, M (2017). Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel-based DNA profiling method. AMB Expr., 7: 1-11.
Drali, R; Berrazeg, M; Zidouni, LL; Hamitouche, F; Abbas, AA; Deriet, A and Mouffok, F (2018). Emergence of mcr-1 plasmid-mediated colistin-resistant Escherichia coli isolates from seawater. Sci. Total Environ., 642: 90-94.
Dziva, F and Stevens, MP (2008). Colibacillosis in poultry: Unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts. Avian Pathol., 37: 355-366.
EUCAST (2022). The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 12.0: 1-110.
Ewers, C; Antão, EM; Diehl, I; Philipp, HC and Wieler, LH (2009). Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential. Appl. Environ. Microbiol., 75: 184-192.
Halfaoui, Z; Menoueri, NM and Bendali, LM (2017). Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria. Vet. World. 10: 830-835.
Hayashi, W; Tanaka, H; Taniguchi, Y; Iimura, M; Soga, E; Kubo, R; Matsuo, N; Kawamura, K; Arakawa, Y; Nagano, Y and Naganoa, N (2019). Acquisition of mcr-1 and cocarriage of virulence genes in avian pathogenic Escherichia coli isolates from municipal wastewater influents in Japan. Appl. Environ. Microbiol., 85: 1-11.
Hoelzer, K; Wong, N; Thomas, J; Talkington, K; Jungman, E and Coukell, A (2017). Antimicrobial drug use in food-producing animals and associated human health risks: What, and how strong, is the evidence? BMC Vet. Res., 13: 1-38.
Johnson, TJ; Siek, KE; Johnson, SJ and Nolan, LK (2006). DNA sequence of a ColV plasmid and prevalence of selected plasmid-encoded virulence genes among avian Escherichia coli strains. J. Bacteriol., 188: 745-758.
Johnson, JR and Stell, AL (2000). Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J. Infect. Dis., 181: 261-272.
Johnson, TJ; Wannemuehler, Y; Doetkott, C; Johnson, SJ; Rosenberger, SC and Nolan, LK (2008). Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool. J. Clin. Microbiol., 46: 3987-3996.
Kim, S; Woo, JH; Kim, N; Kim, MH; Kim, SY; Son, JH; Moon, DC; Lim, SK; Shin, M and Lee, JC (2019). Characterization of chromosome-mediated colistin resistance in Escherichia coli isolates from livestock in Korea. Infect. Drug Resist., 12: 3291-3299.
Liu, YY; Wang, Y; Walsh, TR; Yi, LX; Zhang, R; Spencer, J; Doi, Y; Tian, G; Dong, B; Huang, X; Yu, LF; Gu, D; Ren, H; Chen, X; Lv, L; He, D; Zhou, H; Liang, Z; Liu, JH and Shen, J (2016). Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: A microbiological and molecular biological study. Lancet Infect. Dis., 16: 161-168.
Loucif, L; Chelaghma, W; Bendjama, E; Cherak, Z; Khellaf, M; Khemri, A and Rolain, JM (2022). Detection of blaOXA-48 and mcr-1 genes in Escherichia coli isolates from pigeon (Columba livia) in Algeria. Microorganisms. 10: 1-11.
Magiorakos, A; Srinivasan, A; Carey, RB; Carmeli, Y; Falagas, ME; Giske, CG; Harbarth, S and Hindler, JF (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol. Infect., 18: 268-281.
Maturana, VG; Fernanda, deP; Camila, C; Pires, MM; de Campos, TA; Nakazato, G; Stheling, EG; Logue, MC; Nolan, LK and Wanderley, DdaS (2011). Subpathotypes of avian pathogenic Escherichia coli (APEC) exist as defined by their syndromes and virulence traits. Open Microbiol. J., 5: 55-64.
Mead, A; Billon-Lotz, C; Olsen, R; Swift, B; Richez, P; Stabler, R and Pelligand, L (2022). Epidemiological prevalence of phenotypical resistances and mobilised colistin resistance in avian commensal and pathogenic E. coli from Denmark, France, The Netherlands, and the UK. Antibiotics. 11: 1-16.
Meguenni, N; Chanteloup, N; Trotereau, A; Ahmed, CA; Bounar-Kechih, S and Schouler, C (2019). Virulence and antibiotic resistance profile of avian Escherichia coli strains isolated from colibacillosis lesions in central of Algeria. Vet. World. 12: 1840-1848.
Meguenni, N; Le Devendec, L; Jouy, E; Le Corvec, M; Bounar-Kechih, S; Bakour, R and Kempf, I (2015). First description of an extended-spectrum cephalosporin-and fluoroquinolone-resistant avian pathogenic Escherichia coli clone in Algeria. Avian Dis., 59: 20-23.
Mellata, M (2013). Human and avian extraintestinal pathogenic Escherichia coli: Infections, zoonotic risks, and antibiotic resistance trends. Foodborne Pathog. Dis., 10: 916-932.
Mohamed, L; Ge, Z; Yuehua, L; Yubin, G; Rachid, K; Mustapha, O; Junwei, W and Karine, O (2018). Virulence traits of avian pathogenic (APEC) and fecal (AFEC) E. coli isolated from broiler chickens in Algeria. Trop. Anim. Health Prod., 50: 547-553.
Olaitan, AO; Chabou, S; Okdah, L; Morand, S and Rolain, JM (2016). Dissemination of the mcr-1 colistin resistance gene. Lancet Infect. Dis., 16: 147.
Pourbakhsh, SA; Boulianne, M; Martineau-Doizé, B and Fairbrother, JM (1997). Virulence mechanisms of avian fimbriated Escherichia coli in experimentally inoculated chickens. Vet. Microbiol., 58: 195-213.
Radwan, IAE; Sayed, H; Salam, H and Abd-alwanis, SAA (2014). Frequency of some virulence associated genes among multidrug-resistant Escherichia coli isolated from septicemic broiler chicken. Int. J. Adv. Res., 2: 867-874.
Rebelo, AR; Bortolaia, V; Kjeldgaard, JS; Pedersen, SK; Leekitcharoenphon, P; Hansen, IM; Guerra, B; Malorny, B; Borowiak, M; Hammerl, JA; Battisti, A; Franco, A; Alba, P; Perrin-Guyomard, A; Granier, SA; de Frutos, C; De Frutos Escobar, C; Malhotra-Kumar, S; Villa, L; Carattoli, A and Hendriksen, RS (2018). Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes. Eurosurveillance. 23: 1-11.
Sarowska, J; Futoma-Koloch, B; Jama-Kmiecik, A; Frej-Madrzak, M; Ksiazczyk, M; Bugla-Ploskonska, G and Choroszy-Krol, I (2019). Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: Recent reports. Gut Pathog., 11: 1-16.
Schouler, C; Schaeffer, B; Brée, A; Mora, A; Dahbi, G; Biet, F; Oswald, E; Mainil, J; Blanco, J and Moulin-Schouleur, M (2012). Diagnostic strategy for identifying avian pathogenic Escherichia coli based on four patterns of virulence genes. J. Clin. Microbiol., 50: 1673-1678.
Touati, M; Hadjadj, L; Berrazeg, M; Baron, SA and Rolain, JM (2020). Emergence of Escherichia coli harbouring mcr-1 and mcr-3 genes in North West Algerian farmlands. J. Glob. Antimicrob. Resist., 21: 132-137.
Touati, A and Mairi, A (2021). Plasmid-determined colistin resistance in the north African countries: A systematic review. Microb. Drug Resist., 27: 121-133. Varga, C; Brash, ML; Slavic, D; Boerlin, P; Ouckama, R; Weis, A; Petrik, M; Philippe, C; Barham, M and Guerin, MT (2018). Evaluating virulence-associated genes and antimicrobial resistance of avian pathogenic Escherichia coli isolates from broiler and broiler breeder chickens in Ontario, Canada. Avian Dis., 62: 291-299.
Vounba, P; Yaghouba, K; Ndiaye, C; Arsenault, J; Fairbrother, JM and Bada Alambédji, R (2018). Molecular characterization of Escherichia coli isolated from chickens with colibacillosis in Senegal. Foodborne Pathog. Dis., 15: 517-525.
Wang, C; Feng, Y; Liu, L; Wei, L; Kang, M and Zong, Z (2020). Identification of novel mobile colistin resistance gene mcr-10. Emerg. Microbes Infect., 9: 508-516.
Xu, X; Sun, Q and Zhao, L (2019). Virulence factors and antibiotic resistance of avian pathogenic Escherichia coli in eastern China. J. Vet. Res., 63: 317-320.
Yanat, B; Machuca, J; Yahia, RD; Touati, A; Pascual, Á and Rodríguez-Martínez, JM (2016). First report of the plasmid-mediated colistin resistance gene mcr-1 in a clinical Escherichia coli isolate in Algeria. Int. J. Antimicrob. Agents. 48: 760-761.
Zhu, XQ; Liu, YY; Wu, R; Xun, H; Sun, J; Li, J; Feng, Y and Liu, JH (2021). Impact of mcr-1 on the development of high level colistin resistance in Klebsiella pneumonia and Escherichia coli. Front. Microbiol., 12: 1-9. | ||
|
آمار تعداد مشاهده مقاله: 416 تعداد دریافت فایل اصل مقاله: 457 |
||