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Motazedian, Nasrin, Azarpira, Negar, Falamarzi, Kimia, Dehghani, Seyed Mohsen, Ataollahi, Maryam, Esfandiari, Elaheh, Dara, Mahintaj, Toobafard, Razieh, Sayadi, Mehrab, Shekarforoush, Seyed Ali, Owji, Seyed Hossein, Malekhosseini, Seyed Ali. (1403). Comparison of Mir122 expression in children with biliary atresia and healthy group. , 13(3), 147-154. doi: 10.22099/mbrc.2024.49649.1950
Nasrin Motazedian; Negar Azarpira; Kimia Falamarzi; Seyed Mohsen Dehghani; Maryam Ataollahi; Elaheh Esfandiari; Mahintaj Dara; Razieh Toobafard; Mehrab Sayadi; Seyed Ali Shekarforoush; Seyed Hossein Owji; Seyed Ali Malekhosseini. "Comparison of Mir122 expression in children with biliary atresia and healthy group". , 13, 3, 1403, 147-154. doi: 10.22099/mbrc.2024.49649.1950
Motazedian, Nasrin, Azarpira, Negar, Falamarzi, Kimia, Dehghani, Seyed Mohsen, Ataollahi, Maryam, Esfandiari, Elaheh, Dara, Mahintaj, Toobafard, Razieh, Sayadi, Mehrab, Shekarforoush, Seyed Ali, Owji, Seyed Hossein, Malekhosseini, Seyed Ali. (1403). 'Comparison of Mir122 expression in children with biliary atresia and healthy group', , 13(3), pp. 147-154. doi: 10.22099/mbrc.2024.49649.1950
Motazedian, Nasrin, Azarpira, Negar, Falamarzi, Kimia, Dehghani, Seyed Mohsen, Ataollahi, Maryam, Esfandiari, Elaheh, Dara, Mahintaj, Toobafard, Razieh, Sayadi, Mehrab, Shekarforoush, Seyed Ali, Owji, Seyed Hossein, Malekhosseini, Seyed Ali. Comparison of Mir122 expression in children with biliary atresia and healthy group. , 1403; 13(3): 147-154. doi: 10.22099/mbrc.2024.49649.1950

Comparison of Mir122 expression in children with biliary atresia and healthy group

Molecular Biology Research Communications
دوره 13، شماره 3، 2024، صفحه 147-154    اصل مقاله (440.29 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2024.49649.1950
نویسندگان
Nasrin Motazedian1؛ Negar Azarpira1؛ Kimia Falamarzi2؛ Seyed Mohsen Dehghani1؛ Maryam Ataollahi1؛ Elaheh Esfandiari1؛ Mahintaj Dara* 3؛ Razieh Toobafard* 1؛ Mehrab Sayadi4؛ Seyed Ali Shekarforoush1؛ Seyed Hossein Owji2، 5؛ Seyed Ali Malekhosseini6
1Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
3Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4Cardiovascular Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
5Otolaryngology Research Center, Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran
6Abu-Ali Sina Organ Transplant Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Biliary atresia (BA) is the primary cause of neonatal jaundice with various pathological mechanisms. Many BA patients may experience progressive liver dysfunction and eventually need a liver transplant. Therefore, identifying potential non-invasive biomarkers for BA is crucial. miR-122, the most abundant microRNA in the liver, plays significant roles in different liver diseases. This study aimed to assess miR-122 levels in BA patients. Eighteen patients with biliary atresia were selected at random from the Shiraz Pediatric Liver Cirrhosis Cohort Study (SPLCCS), along with 18 healthy controls. Blood samples were collected, and biochemical parameters (such as liver function tests) were measured. Quantitative reverse-transcription PCR (RT-PCR) was conducted on serum samples from both the case and control groups to analyze miR-122 levels. The study results indicated that serum miR-122 expression in BA patients was elevated compared to the control group, although it did not reach statistical significance. Additionally, no correlation was found between miR-122 expression and serum levels of liver enzymes or other laboratory findings in BA cases. miR-122 could be a potential target for diagnosing BA; however, further research with a larger population is necessary to determine if miR-122 could serve as a useful biomarker for diagnosing BA.
کلیدواژه‌ها
Micro RNA؛ miR-122؛ Biliary atresia؛ Liver
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