| تعداد نشریات | 24 |
| تعداد شمارهها | 912 |
| تعداد مقالات | 8,158 |
| تعداد مشاهده مقاله | 15,836,140 |
| تعداد دریافت فایل اصل مقاله | 13,332,052 |
Isolation and molecular characterization of infectious bursal disease virus circulating in western and central India | ||
| Iranian Journal of Veterinary Research | ||
| دوره 24، شماره 4 - شماره پیاپی 85، 2023، صفحه 345-350 اصل مقاله (500.07 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2023.47037.6774 | ||
| نویسندگان | ||
| A. A. Agnihotri1؛ S. P. Awandkar* 2؛ R. C. Kulkarni3؛ S. G. Chavhan4؛ R. D. D Suryawanshi5؛ A. Mohan6؛ M. B. Kulkarni2؛ V. G. Chavan1 | ||
| 1Post-graduate Student in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary & Animal Sciences, Maharashtra Animal & Fishery Sciences University, Udgir 423517, India | ||
| 2Department of Veterinary Microbiology, College of Veterinary & Animal Sciences, Maharashtra Animal & Fishery Sciences University, Udgir 423517, India | ||
| 3Department of Poultry Science, College of Veterinary & Animal Sciences, Maharashtra Animal & Fishery Sciences University, Udgir 423517, India | ||
| 4Department of Veterinary Pathology, College of Veterinary & Animal Sciences, Maharashtra Animal & Fishery Sciences University, Udgir 423517, India | ||
| 5Department of Veterinary Public Health, College of Veterinary & Animal Sciences, Maharashtra Animal & Fishery Sciences University, Udgir 423517, India | ||
| 6Department of Veterinary Medicine, College of Veterinary & Animal Sciences, Maharashtra Animal & Fishery Sciences University, Udgir 423517, India | ||
| چکیده | ||
| Background: Infectious bursal disease (IBD) caused substantial economic loss in central and western India during 2020 and 2021. Aims: The study was conducted to characterize IBD virus (IBDV) from field outbreaks. Methods: The study was conducted on 360 samples from 42 poultry flocks. The samples were subjected to histopathology and molecular detection, followed by phylogenetic typing of the partial VP2 gene. Results: The mortality ranged from 15.25 to 60.18%. The necropsy showed hemorrhages on thigh muscles, mottled spleen, swelling of kidneys and bursae with hemorrhages, and cheesy exudate. Histopathology revealed extensive necrosis and depletion of follicular lymphoid cells within the cortex and medulla, along with widespread hemorrhages, edema, and cystic cavities in the bursa. The field isolates showed cytopathic effects in the seventh passage. The cytopathic effects included swelling, rounding, granulation of cytoplasm around the nucleus, fragmentation of the infected cells, and detachment. The reverse transcription-polymerase chain reaction amplified 664 bp partial VP2 gene. The phylogenetic analysis identified 19 field isolates as very virulent IBDVs (vvIBDVs) and three as classical strains. Conclusion: The results indicated that different vvIBDV strains are involved in disease outbreaks in central and western India. | ||
| کلیدواژهها | ||
| IBDV؛ India؛ Isolation؛ Phylogeny | ||
| مراجع | ||
|
Awandkar, SP; Tembhurne, PA; Kesharkar, JA; Kurkure, NV; Chaudhari, SP; Bonde, SW and Ingle, VC (2018). Identification and characterization of a novel infectious bursal disease virus from outbreaks in Maharashtra Province of India. Vet. World. 11: 1516-1525.
Blakey, J (2023). Infectious bursal disease in poultry. In: Abuelo, A; Brutlag, A; Carnevale, J; Dart, A; Davidson, G; Davis, JL; Eide, M; Ramirez, A and Swayne, DE (Eds.), MSD veterinary manual. (11th Edn.), USA. Available through https://www.msdvetmanual.com/poultry/ Infectious -bursal-disease/Infectious-bursal-disease-in-poultry (viewed on 02 October 2023).
Camilotti, E; Moraes, LBD; Furian, TQ; Borges, KA; Moraes, HLD and Salle, CTP (2016). Infectious bursal disease: pathogenicity and immunogenicity of vaccines. Braz. J. Poultry Sci., 18: 303-308.
Dey, S; Pathak, DC; Ramamurthy, N; Maity, HK and Chellappa, MM (2019). Infectious bursal disease virus in chickens: prevalence, impact, and management strategies. Vet. Med. Res., 10: 85-97.
Economic Survey, GOI (2021). Allied sectors: Animal husbandry, dairying and fisheries. Economic survey 2020-21. (1st Edn.), Ministry of Finance, Government of India. P: 614. Available through https://www.indiabudget.gov.in/ economicsurvey/ebook_es2021/index.html#p=614 (viewed 17 October 2023).
Escaffre, O; Le Nouën, C; Amelot, M; Ambroggio, X; Ogden, KM; Guionie, O; Toquin, D; Müller, H; Islam, MR and Eterradossi, N (2013). Both genome segments contribute to the pathogenicity of very virulent infectious bursal disease virus. J. Virol., 87: 2767-2780.
Freshney, RI (2016). Primary culture. In: Freshney, RI (Eds.), Culture of animal cells: a manual of basic technique and specialized applications. (7th Edn.), Hoboken, New Jersey, John Wiley and Sons, Inc., PP: 395-427.
Jiang, N; Wang, Y; Zhang, W; Niu, X; Gao, Y; Gao, L; Li, K; Cui, H; Liu, A; Pan, Q and Liu, C (2021). Naturally occurring mutated infectious bursal disease virus of genotype A8B1 associated with bursa damage in China. Virus Res., 302: 198498. doi: 10.1016/j.virusres.2021. 198498.
Kumar, A and Umapathi, V (2011). Sequence analysis of a part of hypervariable region of VP2 gene of chicken embryo fibroblast adapted Infectious Bursal Disease virus isolates of Uttarakhand. J. Biotechnol., Bioinfo. and Bioeng., 1: 109-118.
Mató, T; Tatár-Kis, T; Felföldi, B; Jansson, DS; Homonnay, Z; Bányai, K and Palya, V (2020). Occurrence and spread of a reassortant very virulent genotype of infectious bursal disease virus with altered VP2 amino acid profile and pathogenicity in some European countries. Vet. Microbiol., 245: 108663. doi: 10.1016/j.vetmic.2020.108663.
Michel, LO; Kimber, ML and Jackwood, DJ (2019). New introduction of a very virulent infectious bursal disease virus in New York, USA. Avian Pathol., 48: 486-491.
Mor, SK; Narang, G; Jindal, N; Mahajan, NK; Sharma, PC and Rakha, NK (2010). Epidemiological studies on infectious bursal disease in broiler chickens in Haryana, India. Int. J. Poult. Sci., 9: 395-400.
Morla, S; Deka, P and Kumar, S (2016). Isolation of novel variants of infectious bursal disease virus from different outbreaks in Northeast India. Microb. Pathog., 93: 131-136.
Pitesky, M; Cataline, K; Crossley, B; Poulos, M; Ramos, G; Willoughby, D; Woolcock, P; Cutler, G; Bland, M; Tran, J and Jackwood, D (2013). Historical, spatial, temporal, and time-space epidemiology of very virulent infectious bursal disease in California: A retrospective study 2008-2011. Avian Dis., 57: 76-82.
Raja, P; Senthilkumar, TMA; Parthiban, M; Thangavelu, A; Gowri, AM; Palanisammi, A and Kumanan, K (2016). Complete genome sequence analysis of a naturally reassorted infectious bursal disease virus from India. Genome Announc., 4: e00709-16.
Rekha, K; Sivasubramanian, C; Chung, CM and Thiruvengadam, M (2014). Growth and replication of infectious bursal disease virus in the DF-1 cell line and chicken embryo fibroblasts. Biomed Res. Int., 2014: 494835. Shinde, RS; Chauhan, HC; Patel, AC; Sharma, KK; Patel, SS; Mohapatra, SK; Shrimali, MD and Chandel, BS (2021). VP2 gene sequencing-based Geno-grouping of infectious bursal disease viruses isolated from Gujarat and Maharashtra state (India). Virus Dis., 18: 1-7.
Singh, J; Banga, HS; Brar, RS; Singh, ND; Sodhi, S and Leishangthem, GD (2015). Histopathological and immunohistochemical diagnosis of infectious bursal disease in poultry birds. Vet. World. 8: 1331-1339.
Singh, Y; Yadav, K; Singh, VK and Kumar, R (2014). Molecular diagnosis and adaptation of highly virulent infectious bursal disease virus on chicken embryo fibroblast cell. Vet. World. 7: 351-355.
Spencer, LT and Bancroft, JD (2013). Tissue processing. In: Suvarna, SK; Layton, C and Bancroft, JD (Eds.), Theory and practice of histological techniques. (7th Edn.), Edinburgh, Churchill Livingstone Publisher. PP: 105-123.
Tammiranta, N; Ek-Kommonen, C; Rossow, L and Huovilainen, A (2018). Circulation of very virulent avian infectious bursal disease virus in Finland. Avian Pathol., 47: 520-525.
Van den Berg, TP; Eterradossi, N; Toquin, D and Meulemans, G (2000). Infectious bursal disease (Gumboro disease). Rev. Sci. Tech. Off. Int. Epiz., 19: 509-543.
Yamaguchi, T; Ogawa, M; Miyoshi, M; Inoshima, Y; Fukushi, H and Hirai, K (1997). Sequence and phylogenetic analyses of highly virulent infectious bursal disease virus. Arch. Virol., 142: 1441-1458. | ||
|
آمار تعداد مشاهده مقاله: 553 تعداد دریافت فایل اصل مقاله: 448 |
||