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Molecular identification and phylogenetic analysis of Macrorhabdus ornithogaster based on the 18S rRNA gene in companion birds of Tehran, Iran | ||
| Iranian Journal of Veterinary Research | ||
| دوره 24، شماره 4 - شماره پیاپی 85، 2023، صفحه 328-334 اصل مقاله (917.6 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2023.47741.6916 | ||
| نویسندگان | ||
| F. Hamian1؛ N. Sheikhi* 2؛ S. Charkhkar2؛ Gh. Nikbakht Brujeni* 3 | ||
| 1Ph.D. Student in Avian Hygiene and Medicine, Department of Clinical Science, College of Veterinary Medicine, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Clinical Science, College of Veterinary Medicine, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Background: Macrorhabdus ornithogaster (MO) is an infectious yeast which can cause acute gastric disturbances in birds. It has a worldwide distribution with a broad host-range of bird species. Aims: Molecular identification and phylogenetic analysis of MO based on the 18S rRNA gene in companion birds of Iran. Methods: A total of 54 stool samples were taken from birds (10 species) suspected of being infected. The presence of MO in collected stool samples was investigated using direct wet mount microscopy. Specific primers were designed to identify the MO 18S rRNA gene by using PCR. PCR products were sequenced and phylogenetic analysis was performed to determine the molecular diversity. Results: The obtained results demonstrated that 44.44% and 59.26% of the samples were diagnosed positive based on the first and second specific primers, respectively. MO was detected in the feces of canary, goldfinch, budgerigar, toucan, and English budge. Phylogenetic analysis revealed that MO sequence data from canaries, finches, and goldfinches had homology with an MO isolated from a German zebra finch. Moreover, MOs from cockatiels, rosy faced love birds, and budgerigars had a high phylogenetic similarity with multiple references, including American budgerigar, Japanese cockatiel, European goldfinch, and German budgerigar. Conclusion: MO exists in many species of Iranian birds, including goldfinches, budgerigars, toucans, and English budgies. As indicated by phylogenetic and polymorphism data analysis, the newly designed specific primers spanning a large portion of 18S rRNA gene of MO, provides additional tool to detect and study the molecular diversity of MO. | ||
| کلیدواژهها | ||
| 18S rRNA؛ Macrorhabdus ornithogaster؛ PCR؛ Phylogenetic | ||
| مراجع | ||
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Abdi-Hachesoo, B; Sharifiyazdi, H; Haddad-Marandi, MR and Kalantari, M (2019). Phylogenetic evaluation of Macrorhabdus ornithogaster isolated from a case of canary (Serinus canaria). Comp. Clin. Path., 28: 275-278.
Baron, HR; Leung, KCL; Stevenson, BC; Gonzalez, MS and Phalen, DN (2019). Evidence of amphotericin b resistance in Macrorhabdus ornithogaster in Australian cage-birds. Med. Mycol., 57: 421-428.
Baron, HR; Stevenson, BC and Phalen, DN (2021). Comparison of in-clinic diagnostic testing methods for Macrorhabdus ornithogaster. J. Avian Med. Surg., 35: 37-44.
Borrelli, L; Dipineto, L; Rinaldi, L; Romano, V; Noviello, E; Menna, LF; Cringoli, G and Fioretti, A (2015). New diagnostic insights for Macrorhabdus ornithogaster infection. J. Clin. Microbiol., 53: 3448-3450.
Cooke, SW (2000). Role of megabacteria in mammals. Vet. Rec., 146: 444-447.
Hannafusa, Y; Bradley, A; Tomaszewski, EE; Libal, MC and Phalen, DN (2007). Growth and metabolic characterization of Macrorhabdus ornithogaster. J. Vet. Diagn. Invest., 19: 256-265.
Huchzermeyer, F and Henton, MM (2000). Megabacteria in mammals. Vet. Rec., 146: 768-776.
Kojima, A; Osawa, N; Oba, M; Katayama, Y; Omatsu, T and Mizutani, T (2022). Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster. J. Vet. Med. Sci., 84: 244-250.
Lanzarot, P; Blanco, JL; Alvarez-Perez, S; Abad, C; Cutuli, MT and Garcia, ME (2013). Prolonged fecal shedding of “megabacteria” (Macrorhabdus ornithogaster) by clinically healthy canaries (Serinus canaria). Med. Mycol., 51: 888-891.
Martins, NRS; Horta, AC; Siqueira, AM; Lopes, SQ; Resende, JS; Jorge, MA; Assis, RA; Martins, NE; Fernandes, AA; Barrios, PR; Costa, TJR and Guimarães, LMC (2006). Macrorhabdus ornithogaster in ostrich, rhea, canary, zebra finch, free range chicken, turkey, guinea-fowl, Columbina pigeon, toucan, chuckar partridge and experimental infection in chicken, Japanese quail and mice. Braz. J. Vet. Res. Anim. Sci., 58: 291-298.
Ozmen, O; Aydogan, A; Haligur, M; Adanir, R; Kose, O and Sahinduran, S (2013). The pathology of Macrorhabdus ornithogaster and Eimeria dunsingi infections in budgerigars (Melopsittacus undulatus). Isr. J. Vet. Med., 68: 218-224.
Paula, IHdL; Linhares, FP; Kanayama, CY; Carvalho, SPde; Bittar, ER; Campos, MTG and Bittar, JFF (2018). Megabacteria (Macrorhabdus ornithogaster) in psittacids kept in commercial establishments in the municipality of Uberaba – MG. Pubvet. 12: 1-4.
Phalen, DN (2014). Update on the diagnosis and management of Macrorhabdus ornithogaster (formerly megabacteria) in avian patients. Vet. Clin. North. Am. Exot. Anim. Pract., 17: 203-210.
Powers, LV; Mitchell, MA and Garner, MM (2019). Macrorhabdus ornithogaster infection and spontaneous proventricular adenocarcinoma in budgerigars (Melopsittacus undulatus). Vet. Pathol., 56: 486-493. Püstow, R and Krautwald-Junghanns, ME (2017). The incidence and treatment outcomes of Macrorhabdus ornithogaster infection in budgerigars (Melopsittacus undulatus) in a veterinary clinic. J. Avian Med. Surg., 31: 344-350.
Razmyar, J; Movassaghi, AR and Rezaee, M (2016). An outbreak of severe Macrorhabdus ornithogaster infection in common canaries (Serinus canarius domesticus), molecular and pathological assay. Iran. J. Vet. Sci. Technol., 8: 64-68.
Robino, P; Ferrocino, I; Rossi, G; Dogliero, A; Alessandria, V; Grosso, L; Galosi, L; Tramuta, C; Cocolin, L and Nebbia, P (2019). Changes in gut bacterial communities in canaries infected by Macrorhabdus ornithogaster. Avian Pathol., 48: 111-120.
Sullivan, PJ; Ramsay, EC; Greenacre, CB; Cushing, AC; Zhu, X and Jones, MP (2017). Comparison of two methods for determining prevalence of Macrorhabdus ornithogaster in a flock of captive budgerigars (Melopsittacus undulatus). J. Avian Med. Surg., 31: 128-131.
Talazadeh, F; Ghorbanpoor, M and Bahadori, Y (2023). Avian gastric yeast (macrorhabdosis) in cockatiel, budgerigar and grey parrot: a focus on the clinical signs, molecular detection and phylogenetic evaluation. Vet. Res. Forum, 14: 281-287.
Tomaszewski, EK; Logan, KS; Snowden, KF; Kurtzman, CP and Phalen, DN (2003). Phylogenetic analysis identifies the 'megabacterium' of birds as a novel anamorphic ascomycetous yeast, Macrorhabdus ornithogaster gen. nov., sp. nov. Int. J. Syst. Evol. Microbiol., 53: 1201-1205. | ||
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