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Bonyadian, M., Moshtaghi, H., Nematalahi, A., Rahimi, E., Akhavan Taheri, M., Karami, S.. (1390). Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR. , 12(3), 252-255. doi: 10.22099/ijvr.2011.74
M. Bonyadian; H. Moshtaghi; A. Nematalahi; E. Rahimi; M. Akhavan Taheri; S. Karami. "Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR". , 12, 3, 1390, 252-255. doi: 10.22099/ijvr.2011.74
Bonyadian, M., Moshtaghi, H., Nematalahi, A., Rahimi, E., Akhavan Taheri, M., Karami, S.. (1390). 'Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR', , 12(3), pp. 252-255. doi: 10.22099/ijvr.2011.74
Bonyadian, M., Moshtaghi, H., Nematalahi, A., Rahimi, E., Akhavan Taheri, M., Karami, S.. Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR. , 1390; 12(3): 252-255. doi: 10.22099/ijvr.2011.74

Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR

Iranian Journal of Veterinary Research
مقاله 11، دوره 12، شماره 3 - شماره پیاپی 36، 2011، صفحه 252-255    اصل مقاله (50.59 K)
نوع مقاله: Short paper
شناسه دیجیتال (DOI): 10.22099/ijvr.2011.74
نویسندگان
M. Bonyadian* 1؛ H. Moshtaghi1؛ A. Nematalahi1؛ E. Rahimi2؛ M. Akhavan Taheri3؛ S. Karami3
1Department of Health and Food Quality Control, Institute of Zoonoses Research, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
2Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
3Graduated from Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
چکیده

The aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains of
Escherichia coli in chicken carcasses by polymerase chain reaction (PCR). In this study 63 strains of E. coli
were isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughter
house of Shahrekord. Polymerase chain reaction assays were used to detect the presence of the genes
encoding heat-stable enterotoxin a (STa), heat-stable enterotoxin b (STb), heat labile toxin (LT) and
Enteroaggregative heat-stable toxin 1 (EAST1). Sixty three out of 110 (57.27%) carcasses were contaminated with E. coli. Six out of 63 (9.52%) harbored the gene for LT, 1 (1.58%) STb, 21 (33.3%) EAST1 and 8 (12.69%) contain both LT and EAST1 genes. None of the strains contain the STa gene. The results indicated that contamination of the chicken carcasses with E. coli in such a level could be a potential hazard for consumers.

کلیدواژه‌ها
Chicken carcasses؛ Escherichia coli؛ ETEC؛ EAggEC؛ PCR
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