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Behrouj, Hamid, Erfani, Mehran, Mokarram, Pooneh. (1403). Examining the expression of low-density lipoprotein receptor (LDLR) and low-density lipoprotein receptor-related protein 6 (LRP6) genes in breast cancer cell lines. , 13(2), 85-88. doi: 10.22099/mbrc.2024.48583.1882
Hamid Behrouj; Mehran Erfani; Pooneh Mokarram. "Examining the expression of low-density lipoprotein receptor (LDLR) and low-density lipoprotein receptor-related protein 6 (LRP6) genes in breast cancer cell lines". , 13, 2, 1403, 85-88. doi: 10.22099/mbrc.2024.48583.1882
Behrouj, Hamid, Erfani, Mehran, Mokarram, Pooneh. (1403). 'Examining the expression of low-density lipoprotein receptor (LDLR) and low-density lipoprotein receptor-related protein 6 (LRP6) genes in breast cancer cell lines', , 13(2), pp. 85-88. doi: 10.22099/mbrc.2024.48583.1882
Behrouj, Hamid, Erfani, Mehran, Mokarram, Pooneh. Examining the expression of low-density lipoprotein receptor (LDLR) and low-density lipoprotein receptor-related protein 6 (LRP6) genes in breast cancer cell lines. , 1403; 13(2): 85-88. doi: 10.22099/mbrc.2024.48583.1882

Examining the expression of low-density lipoprotein receptor (LDLR) and low-density lipoprotein receptor-related protein 6 (LRP6) genes in breast cancer cell lines

Molecular Biology Research Communications
دوره 13، شماره 2، 2024، صفحه 85-88    اصل مقاله (201.6 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2024.48583.1882
نویسندگان
Hamid Behrouj1، 2؛ Mehran Erfani3؛ Pooneh Mokarram* 4
1Behbahan FDepartment of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iranaculty of Medical Sciences, Behbahan, Iran
2Behbahan Faculty of Medical Sciences, Behbahan, Iran
3Department of Biochemistry, School of Medicine Hormozgan University of Medical Sciences, Hormozgan, Iran
4Autophagy Research Center, Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Cholesterol and the Wnt/β-catenin pathway have an effective role in the proliferation, survival, drug resistance, immune exhaustion, and metastasis of all types of cancer cells. Considering the role of LDLR and LRP6 proteins in cholesterol uptake by cells and activation of Wnt/β-catenin pathway, this study aims to examine the gene expression of LDLR and LRP6 in cell lines of breast cancer. Human breast cancer cell lines MCF7, MD468 and SKBR3 were cultured in suitable conditions and after extracting total RNA from them, real-Time PCR was used to measure the levels of gene expression for LDLR and LRP6. Our results showed that the expression of LDLR and LRP6 genes is significantly increased in MCF7 and MD468 cells compared to SKBR3 cells. These results suggest that LRP6 and LDLR can be considered as a therapeutic target in tumors that have a genetic profile similar to MCF7 and MD468 cells
کلیدواژه‌ها
Breast cancer؛ Cholesterol؛ Wnt/β-catenin signaling؛ LDLR؛ LRP6
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