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Molecular detection of Brucella abortus and Brucella melitensis in domestic ruminants and their ticks in selected areas of western Iran | ||
| Iranian Journal of Veterinary Research | ||
| دوره 24، شماره 3 - شماره پیاپی 84، 2023، صفحه 270-275 اصل مقاله (311.41 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2023.47192.6806 | ||
| نویسندگان | ||
| M. Moravedji1؛ M. Beig2؛ N. Baseri3؛ M. Rahravani* 4؛ M. Latifian5؛ S. Esmaeili* 6 | ||
| 1Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran | ||
| 2Ph.D. Student in Medical Bacteriology, Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran | ||
| 3Ph.D. in Medical Bacteriology, Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran | ||
| 4Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran | ||
| 5BSc in Medical Laboratory Sciences, National Reference Laboratory for Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar Ahang, Hamadan, Iran | ||
| 6Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran | ||
| چکیده | ||
| Background: Brucellosis is one of the most important zoonotic diseases caused by Gram-negative bacteria belonging to the genus Brucella. Detection of Brucella species in different countries is of utmost importance. Aims: This study aimed to detect Brucella abortus and Brucella melitensis in domestic ruminant blood samples and their ticks in western Iran. Methods: Sampling was conducted on ruminants from August to September 2020 in four different counties of Kurdistan Province, including Divandareh, Marivan, Baneh, and Sanandaj. Totally, 250 blood samples were collected from 250 small ruminants. There were no ticks on the skin of six (2.4%) ruminants, and 244 ticks were isolated from 244 animals. After genomic DNA extraction from all the collected samples, quantitative polymerase chain reaction (qPCR) was performed to detect IS711 gene. Results: Based on qPCR results, Brucella genus was detected in two blood samples (0.8%) from female sheep and four ticks (1.6%) from male sheep, including three Dermacentor marginatus (1.22%) and one Rhipicephalus turanicus (0.4%). Although B. melitensis was not detected in any tick or blood sample, one tick sample (D. marginatus) was positive for B. abortus. Conclusion: Considering the positivity of ticks for brucellosis in this study, there is a possibility of Brucella transmission from infected ticks to humans and animals through tick bites, nevertheless, in order to identify the Brucella transmission relationship between ticks and animals, serological tests should be used in future studies. | ||
| کلیدواژهها | ||
| B. abortus؛ B. melitensis؛ Dermacentor marginatus؛ Rhipicephalus turanicus؛ Ruminant | ||
| مراجع | ||
|
Ahmed, ME; Ibrahim, A and Abd El-Rahman, MM (2020). Microbiological and immunological studies on brucellosis in a hospital in Al-Madinah Al-Munawarah. JSRMBS., 1: 24-44.
Balinandi, S; Chitimia-Dobler, L; Grandi, G; Nakayiki, T; Kabasa, W; Bbira, J; Lutwama, J; Bakkes, D; Malmberg, M and Mugisha, L (2020). Morphological and molecular identification of ixodid tick species (Acari: Ixodidae) infesting cattle in Uganda. Parasitol. Res., 119: 2411-2420.
Bounaadja, L; Albert, D; Chénais, B; Hénault, S; Zygmunt, MS; Poliak, S and Garin-Bastuji, B (2009). Real-time PCR for identification of Brucella spp: a comparative study of IS711, bcsp31 and per target genes. Vet. Microbiol., 137: 156-164.
Cull, B (2021). Potential for online crowdsourced biological recording data to complement surveillance for arthropod vectors. PLoS One. 16: e0250382.
Dadar, M; Alamian, S; Behrozikhah, A; Yazdani, F; Kalantari, A; Etemadi, A and Whatmore, AM (2019). Molecular identification of Brucella species and biovars associated with animal and human infection in Iran, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. Ret. Ves. Forum., 10: 315-321.
Dadar, M; Tabibi, R; Alamian, S; Caraballo-Arias, Y; Mrema, EJ; Mlimbila, J; Chandrasekar, S; Dzhusupov, K; Sulaimanova, C and Alekesheva, LZ (2022). Safety concerns and potential hazards of occupational brucellosis in developing countries: A review. J. Public Health. 31: 1681-1690.
Delam, H; Keshtkaran, Z; Rezaei, B; Soufi, O and Bazrafshan, MR (2022). Changing patterns in epidemio-logy of brucellosis in the South of Iran (2015-2020): Based on Cochrane-Armitage Trend test. Ann. Glob. Health. 88: 11, 1-8.
Egyed, L; Elo, P; Sreter-Lancz, Z; Szell, Z; Balogh, Z and Sreter, T (2012). Seasonal activity and tick-borne pathogen infection rates of Ixodes ricinus ticks in Hungary. Ticks Tick Borne Dis., 3: 90-94.
Franc, K; Krecek, R; Häsler, B and Arenas-Gamboa, A (2018). Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action. BMC Public Health. 18: 1-9.
Golshani, M and Buozari, S (2017). A review of brucellosis in Iran: epidemiology, risk factors, diagnosis, control, and prevention. Iran Biomed. J., 21: 349-359.
Hinić, V; Brodard, I; Thomann, A; Cvetnić, Ž; Makaya, P; Frey, J and Abril, C (2008). Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J. Microbiol. Methods. 75: 375-378.
Hosseini-Chegeni, A; Tavakoli, M; Telmadarraiy, Z; Sedaghat, MM and Faghihi, F (2017). Detection of a Brucella-like (Alphaproteobacteria) bacterium in boophilus spp. (Acari: Ixodidae) from Iran. JoMMID., 5: 66-68.
Huang, T; Zhang, J; Sun, C; Liu, Z; He, H and Wu, J (2020). A novel arthropod host of brucellosis in the arid steppe ecosystem. Front. Vet. Sci., 7: 566253.
Islam, MS; Islam, MA; Rahman, MM; Islam, K; Islam, MM; Kamal, MM and Islam, MN (2023). Presence of Brucella spp. in milk and dairy products: A comprehensive review and its perspectives. J. Food Qual., 2932883: 1-19.
Jia, W; Chen, S; Chi, S; He, Y; Ren, L and Wang, X (2022). Recent progress on tick-borne animal diseases of veterinary and public health significance in China. Viruses. 14: 355.
Khan, MZ; Usman, T; Sadique, U; Qureshi, MS; Hassan, MF; Shahid, M and Khan, A (2017). Molecular characterization of Brucella abortus and Brucella melitensis in cattle and humans at the North West of Pakistan. Pak. Vet. J., 37: 360-363.
Lah, EFC; Yaakop, S; Ahamad, M; George, E and Nor, SM (2016). Precise identification of different stages of a tick, Ixodes granulatus Supino, 1897 (Acari: Ixodidae). Asian Pac. J. Trop. Biomed., 6: 597-604.
Li, Y; Wen, X; Li, M; Moumouni, PFA; Galon, EM; Guo, Q; Rizk, MA; Liu, M; Li, J and Ji, S (2020). Molecular detection of tick-borne pathogens harbored by ticks collected from livestock in the Xinjiang Uygur Autonomous Region, China. Ticks Tick Borne Dis., 11: 101478.
Miller, R; Nakavuma, J; Ssajjakambwe, P; Vudriko, P; Musisi, N and Kaneene, J (2016). The prevalence of brucellosis in cattle, goats and humans in rural Uganda: a comparative study. Transbound Emerg. Dis., 63: e197-e210.
Mu, J; Li, Q; Yan, X; Mao, X; Shi, Y; Qin, Y; Liu, C and Wang, W (2022). Detection of Brucella S2 vaccine strain by a loop-mediated isothermal amplification (LAMP) method. Front. Cell. Infect., 12: 1023243.
Namgyal, J; Tenzin, T; Checkley, S; Lysyk, TJ; Rinchen, S; Gurung, RB; Dorjee, S; Couloigner, I and Cork, SC (2021). A knowledge, attitudes, and practices study on ticks and tick-borne diseases in cattle among farmers in a selected area of eastern Bhutan. PloS One. 16: e0247302.
Omitola, OO and Taylor-Robinson, AW (2020). Emerging and re-emerging bacterial zoonoses in Nigeria: current preventive measures and future approaches to intervention. Heliyon. 6: e04095.
Pal, M; Kerorsa, GB; Desalegn, C and Kandi, V (2020). Human and animal brucellosis: A comprehensive review of biology, pathogenesis, epidemiology, risk factors, clinical signs, laboratory diagnosis, public health significance, economic importance, prevention and control. Am. J. Infect. Dis., 8: 118-126.
Rossetti, CA; Arenas-Gamboa, AM and Maurizio, E (2017). Caprine brucellosis: A historically neglected disease with significant impact on public health. PloS Negl. Trop. Dis., 11: e0005692.
Saeed, U; Ali, S; Khan, TM; El-Adawy, H; Melzer, F; Khan, AU; Iftikhar, A and Neubauer, H (2019). Seroepidemiology and the molecular detection of animal brucellosis in Punjab, Pakistan. Microorganisms. 7: 449.
Wakjira, BS and Dilba, GM (2010). Review on serological and molecular diagnostics techniques of brucellosis. Croat. Med. J., 51: 320-326.
Wang, Q; Zhao, S; Lu, J and Wang, Y (2019). Transmission of tick-borne Brucella. J. Pathog. Biol., 14: 238-243.
Wang, Q; Zhao, S; Wureli, H; Xie, S; Chen, C; Wei, Q; Cui, B; Tu, C and Wang, Y (2018). Brucella melitensis and B. abortus in eggs, larvae and engorged females of Dermacentor marginatus. Ticks Tick Borne Dis., 9: 1045-1048.
Whatmore, AM; Koylass, MS; Muchowski, J; Edwards-Smallbone, J; Gopaul, KK and Perrett, LL (2016). Extended multilocus sequence analysis to describe the global population structure of the genus Brucella: phylogeography and relationship to biovars. Front Microbiol., 7: 2049.
Xu, N; Qu, C; Sai, L; Wen, S; Yang, L; Wang, S; Yang, H; Liu, H and Wang, G (2023). Evaluating the efficacy of serological testing of clinical specimens collected from patients with suspected brucellosis. PLoS Negl. Trop. Dis., 17: e0011131.
Zeinali, M; Doosti, S; Amiri, B; Gouya, MM and Godwin, GN (2022). Trends in the epidemiology of brucellosis cases in Iran during the last decade. Iran. J. Public Health. 51: 2791-2798.
Zeng, W; Li, Z; Jiang, T; Cheng, D; Yang, L; Hang, T; Duan, L; Zhu, D; Fang, Y and Zhang, Y (2022). Identification of bacterial communities and tick-borne pathogens in Haemaphysalis spp. collected from Shanghai, China. Infect. Dis. Trop. Med., 7: 413.
Zhang, K; Li, A; Wang, Y; Zhang, J; Chen, Y; Wang, H; Shi, R and Qiu, Y (2021). Investigation of the presence of Ochrobactrum spp. and Brucella spp. in Haemaphysalis longicornis. Ticks Tick Borne Dis., 12: 101588.
Zhao, GP; Wang, YX; Fan, ZW; Ji, Y; Liu, MJ; Zhang, WH; Li, XL; Zhou, SX; Li, H and Liang, S (2021). Mapping ticks and tick-borne pathogens in China. Nat. Commun., 12: 1075. | ||
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