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Virulence genes and antibiotic resistance profiles of Staphylococcus aureus isolated from bovine mastitis milk samples | ||
| Iranian Journal of Veterinary Research | ||
| دوره 24، شماره 3 - شماره پیاپی 84، 2023، صفحه 258-264 اصل مقاله (326.19 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2023.45279.6652 | ||
| نویسندگان | ||
| Z. Hemati1؛ L. Abdolmohammadi Khiav* 2؛ A. Zahmatkesh3 | ||
| 1Department of Pathobiology, School of Veterinary Medicine, ShahreKord University, ShahreKord, Iran | ||
| 2Ph.D. in Bacteriology, Department of Anaerobic Bacterial Vaccine Production and Research, Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 3Department of Anaerobic Bacterial Vaccine Production and Research, Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| Background: The increasing importance of antibiotic resistance shows the need for determining indices of the epidemiology of infection. Aims: This study aimed to determine the virulence genes and antibiotic resistance profiles of Staphylococcus aureus isolated from bovine mastitis cases. Methods: A total of 200 cattle were selected based on California Mastitis Test (CMT) results, and the samples were cultured in the laboratory. Grown colonies were examined by conventional phenotypic methods and confirmed using PCR amplification of 16S rRNA gene. The prevalence of the virulence genes was also defined. The results of phenotypic and molecular tests were compared using SPSS software by McNemar test. Then, the confirmed isolates were tested for antibiotic susceptibility using the disc diffusion method. Results: Of the 200 positive CMT cattle, 24 animals were positive for S. aureus and confirmed using 16S rRNA gene amplification. Statistical analysis showed that the phenotypic and genotypic tests of hemolysin genes were not significantly different (P>0.01). PCR analysis revealed the presence of coa and clfa genes in more than half of the cases. Overall, nine genetic profiles of virulence factors were found among S. aureus isolates. The highest and lowest resistance rates were against penicillin and gentamicin, respectively. Conclusion: Our findings showed a high rate of antibiotic resistance. So, accurate and fast diagnosis and antimicrobial susceptibility tests should be considered before prescribing the drugs. | ||
| کلیدواژهها | ||
| Antibiotic resistance؛ Mastitis؛ Staphylococcus aureus؛ Virulence genes | ||
| مراجع | ||
|
Abrahmsén, M; Persson, Y; Kanyima, BM and Båge, R (2014). Prevalence of subclinical mastitis in dairy farms in urban and peri-urban areas of Kampala, Uganda. Trop. Anim. Health Prod., 46: 99-105.
Ariyanti, D; Salasia, SIO and Tato, S (2011). Characterization of haemolysin of Staphylococcus aureus isolated from food of animal origin. Indones. J. Biotechnol., 16: 32-37.
Bahrami, A; shamsi, M; Bahrami, A; Soltani, S; Shokri, A; Ahmadi, M; Talebimaymand, F and Abasian, L (2016). Staphylococcus aureus isolated from bovine mastitis milk livestock and the study of antibiotic resistance. J. Ilam Uni. Med. Sci., 24: 18-25.
Brînda, M; Herman, V and Faur, B (2010). Antimicrobial sensitivity of some Staphylococcus aureus strains from bovine mastitis. Lucr. Ştiinţ. Ser. Med. Vet., 43: 102-105.
Campos, B; Pickering, AC; Rocha, LS; Aguilar, AP; Fabres-Klein, MH; de Oliveira Mendes, TA; Fitzgerald, JR and de Oliveira Barros Ribon, A (2022). Diversity and pathogenesis of Staphylococcus aureus from bovine mastitis: current understanding and future perspectives. BMC Vet. Res., 18: 115. https://doi.org/10.1186/s12917-022-03197-5.
Chopra, I and Roberts, M (2001). Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol. Mol. Biol. Rev., 65: 232-260.
Ciftci, A; Onuk, EE; Çiftci, G; Fındık, A; Söğüt, MU; Didinen, BI; Aksoy, A and Üstünakın, K (2009). Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis. Braz. J. Microbiol., 40: 254-261.
CLSI (2020). Performance standards for antimicrobial susceptibility testing. 30th Edn., CLSI Supplement M100, Wayne, PA: Clinical and Laboratory Standards Institute.
Da Silva, RE; Boechat, JUD; Martins, JCD; Ferreira, WPB; Sequera, AP and Da silva, N (2005). Hemolysin production by Staphylococcus aureus species isolated from mastitic goat milk in Brazilian dairy herd. Small Rum. Res., 56: 271-275.
Elsayed, MS and Dawoud, MA (2015). Phenotypic and genotypic detection of virulence factors of Staphylococcus aureus isolated from clinical and subclinical mastitis in cattle and water buffaloes from different farms of Sadat City in Egypt. Vet. World. 8: 1051-1058.
Franco, JC; González, L; Gómez, SC; Carrillo, JM and Ramírez, JJ (2008). Virulence factors analysis of Staphylococcus aureus isolated from bovine mastitis in México. e-Gnosis., 6: 1-9.
Gao, J; Ferreri, M; Liu, XQ; Chen, LB; Su, JL and Han, B (2011). Development of multiplex polymerase chain reaction assay for rapid detection of Staphylococcus aureus and selected antibiotic resistance genes in bovine mastitic milk samples. J. Vet. Diagn. Invest., 23: 894-901.
Gianneechini, R; Concha, C; Rivero, R; Delucci, I and Moreno López, J (2002). Occurrence of clinical and sub-clinical mastitis in dairy herds in the West Littoral Region in Uruguay. Acta Vet. Scand., 43: 1-10.
Girma, A and Tamir, D (2022). Prevalence of bovine mastitis and its associated risk factors among dairy cows in Ethiopia during 2005-2022: a systematic review and meta-analysis. Vet. Med. Int., 4: 1-19. https://doi.org/10.1155/2022/ 7775197.
Gonçalves, JL; Kamphuis, C; Martins, C; Barreiro, JR; Tomazi, T; Gameiro, AH; Hogeveen, H and dos Santos, MV (2018). Bovine subclinical mastitis reduces milk yield and economic return. Livest Sci., 210: 25-32. https://doi. org/10.1016/j.livsci.2018.01.016.
Güler, L; Ok, U; Gündüz, K; Gülcü, Y and Hadimli, HH (2005). Antimicrobial susceptibility and coagulase gene typing of Staphylococcus aureus isolated from bovine clinical mastitis cases in Turkey. J. Dairy Sci., 88: 3149-3154.
Hair, PS; Echague, CG; Sholl, AM; Watkins, JA; Geoghegan, JA; Foster, TJ and Cunnion, KM (2010). Clumping factor A interaction with complement factor I increases C3b cleavage on the bacterial surface of Staphylococcus aureus and decreases complement-mediated phagocytosis. Infect. Immun., 78: 1717-1727.
Hashemi, M (2015). Prevalence of antibiotic resistance among bacterial pathogens isolated from dairy cows with mastitis in Fars province. Vet. J., 112: 85-93.
Hashemi, M; Kafi, M and Safdarian, M (2011). The prevalence of clinical and subclinical mastitis in dairy cows in the central region of Fars province, south of Iran. Iran. J. Vet. Res., 12: 236-241.
Hosseinzadeh, S and Saei, HD (2014). Staphylococcal species associated with bovine mastitis in the North West of Iran: Emerging of coagulase-negative staphylococci. Int. J. Vet. Sci. Med., 2: 27-34.
Huys, G; Cnockaert, M; Bartie, K; Oanh, DT; Phuong, NT; Somsiri, T; Chinabut, S and Yussoff, FM (2005). Intra-and interlaboratory performance of antibiotic disk-diffusion-susceptibility testing of bacterial control strains of relevance for monitoring aquaculture environments. Dis. Aquat. Organ., 66: 197-204.
Jena, MK (2020). Bovine mastitis: A major concern of dairy industry. Eur. J. Mol. Clin. Med., 7: 3970-3976.
Kalorey, DR; Shanmugam, Y; Kurkure, NV; Chousalkar, KK and Barbuddhe, SB (2007). PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases. J. Vet. Sci., 8: 151-154.
Käppeli, N; Morach, M; Corti, S; Eicher, C; Stephan, R and Johler, S (2019). Staphylococcus aureus related to bovine mastitis in Switzerland: Clonal diversity, virulence gene profiles, and antimicrobial resistance of isolates collected throughout 2017. J. Dairy Sci., 102: 3274-3281.
Karimuribo, E; Fitzpatrick, JL; Swai, ES; Bell, C; Bryant, MJ; Ogden, NH; Kambarage, DM and French, NP (2008). Prevalence of subclinical mastitis and associated risk factors in smallholder dairy cows in Tanzania. Vet. Rec., 163: 16-21.
Kateete, DP; Namazzi, S; Okee, M; Okeng, A; Baluku, H; Musisi, NL; Katabazi, FA; Joloba, ML; Ssentongo, R and Najjuka, FC (2011). High prevalence of methicillin resistant Staphylococcus aureus in the surgical units of Mulago hospital in Kampala, Uganda. BMC Res. Notes. 4: 1-5.
Kerro Dego, O and Tareke, F (2003). Bovine mastitis in selected areas of southern Ethiopia. Trop. Anim. Health Prod., 35: 197-205.
Kibebew, K (2017). Bovine mastitis: A review of causes and epidemiological point of view. J. Biol. Agric. Health. 7: 1-14.
Kumar, R; Yadav, B and Singh, R (2010). Genetic determinants of antibiotic resistance in Staphylococcus aureus isolates from milk of mastitic crossbred cattle. Curr. Microbiol., 60: 379-386.
Lalita, S; Amit, KV; Amit, K; Anu, R and Rajesh, N (2015). Incidence and pattern of antibiotic resistance of Staphylococcus aureus isolated from clinical and subclinical mastitis in cattle and buffaloes. Asian J. Anim. Sci., 9: 100-109.
Leitner, G; Krifucks, O; Kiran, MD and Balaban, N (2011). Vaccine development for the prevention of staphylococcal mastitis in dairy cows. Vet. Immunol. Immunopathol., 142: 25-35.
Meiri-Bendek, I; Lipkin, E; Friedmann, A; Leitner, G; Saran, A; Friedman, S and Kashi, Y (2002). A PCR-based method for the detection of Streptococcus agalactiae in milk. J. Dairy Sci., 85: 1717-1723.
Momtaz, H; Rahimi, E and Tajbakhsh, E (2010). Detection of some virulence factors in Staphylococcus aureus isolated from clinical and subclinical bovine mastitis in Iran. Afr. J. Biotechnol., 9: 3753-3758.
Monday, SR and Bohach, GA (1999). Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates. J. Clin. Microbiol., 37: 3411-3414.
Moroni, P; Pisoni, G; Antonini, M; Villa, R; Boettcher, P and Carli, S (2006). Antimicrobial drug susceptibility of Staphylococcus aureus from subclinical bovine mastitis in Italy. J. Dairy Sci., 89: 2973-2976.
Mubarack, HM; Doss, A; Vijayasanthi, M and Venkataswamy, R (2012). Antimicrobial drug sus-ceptibility of Staphylococcus aureus from subclinical bovine mastitis in Coimbatore, Tamilnadu, South India. Vet. World. 5: 352-355.
Oliveira, L; Langoni, H; Hulland, C and Ruegg, P (2012). Minimum inhibitory concentrations of Staphylococcus aureus recovered from clinical and subclinical cases of bovine mastitis. J. Dairy Sci., 95: 1913-1920.
Petersson-Wolfe, CS; Mullarky, IK and Jones, GM (2010). Staphylococcus aureus mastitis: cause, detection, and control. College of Agriculture and Life Sciences, Virginia Polytechnic Institute and State University. PP: 1-7.
Rusenova, N; Gebreyes, W; Koleva, M; Mitev, J; Penev, T; Vasilev, N and Mitev, T (2013). Comparison of three methods for routine detection of Staphylococcus aureus isolated from bovine mastitis. Kafkas Univ. Vet. Fak. Derg., 19: 709-712.
Sahebekhtiari, N; Nochi, Z; Eslampour, M; Dabiri, H; Bolfion, M; Taherikalani, M; Khoramian, B and Zali, M (2011). Characterization of Staphylococcus aureus strains isolated from raw milk of bovine subclinical mastitis in Tehran and Mashhad. Acta Microbiol. Immunol. Hung., 58: 113-121.
Sargeant, J; Leslie, K; Shirley, J; Pulkrabek, B and Lim, G (2001). Sensitivity and specificity of somatic cell count and California Mastitis Test for identifying intramammary infection in early lactation. J. Dairy Sci., 84: 2018-2024.
Schmitt, J; Joost, I; Skaar, EP; Herrmann, M and Bischoff, M (2012). Haemin represses the haemolytic activity of Staphylococcus aureus in an Sae-dependent manner. Microbiology. 158: 2619-2631.
Sedaghat, H; Esfahani, BN; Halaji, M; Jazi, AS; Mobasherizadeh, S; Havaei, SR; Emaneini, M and Havaei, SA (2018). Genetic diversity of Staphylococcus aureus strains from a teaching hospital in Isfahan, Iran: The emergence of MRSA ST639-SCCmec III and ST343-SCCmec III. Iran. J. Microbiol., 10: 82-89.
Sedaghat, H; Nasr Esfahani, B; Mobasherizadeh, S; Sallari Jazi, A; Halaji, M; Sadeghi, P; Emaneini, M and Havaei, SA (2017). Phenotypic and genotypic characteri-zation of macrolide resistance among Staphylococcus aureus isolates in Isfahan, Iran. Iran. J. Microbiol., 9: 264-270.
Tabor, DE; Yu, L; Mok, H; Tkaczyk, C; Sellman, BR; Wu, Y; Oganesyan, V; Slidel, T; Jafri, H; McCarthy, M; Bradford, P and Esser, MT (2016). Staphylococcus aureus alpha-toxin is conserved among diverse hospital respiratory isolates collected from a global surveillance study and is neutralized by monoclonal antibody MEDI4893. Antimicrob. Agents Chemother., 60: 5312-5321. Tarazona-Manrique, L; Villate-Hernández, J and Andrade-Becerra, R (2019). Bacterial and fungal infectious etiology causing mastitis in dairy cows in the highlands of Boyacá (Colombia). Rev. Med. Vet. Zoot., 66: 208-218.
Tong, SY; Davis, JS; Eichenberger, E; Holland, TL and Fowler, VGJr (2015). Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin. Microbiol. Rev., 28: 603-661.
Xiaohong, W and Yanjun, Z (2011). Study on the hemolysin phenotype and the genetype distribution of Staphyloccocus aureus caused bovine mastitis in Shandong dairy farms. Intern. J. Appl. Res. Vet. Med., 9: 416-421. | ||
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