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Gopalakrishnan, Sivakumar, Pandi, Anitha, Arumugam, Paramasivam, Priyadharsini Jayaseelan, Vijayashree. (1402). MicroRNAs targeting CDKN2A gene as a potential prognostic marker in head and neck squamous cell carcinoma. , 13(1), 21-27. doi: 10.22099/mbrc.2023.48081.1853
Sivakumar Gopalakrishnan; Anitha Pandi; Paramasivam Arumugam; Vijayashree Priyadharsini Jayaseelan. "MicroRNAs targeting CDKN2A gene as a potential prognostic marker in head and neck squamous cell carcinoma". , 13, 1, 1402, 21-27. doi: 10.22099/mbrc.2023.48081.1853
Gopalakrishnan, Sivakumar, Pandi, Anitha, Arumugam, Paramasivam, Priyadharsini Jayaseelan, Vijayashree. (1402). 'MicroRNAs targeting CDKN2A gene as a potential prognostic marker in head and neck squamous cell carcinoma', , 13(1), pp. 21-27. doi: 10.22099/mbrc.2023.48081.1853
Gopalakrishnan, Sivakumar, Pandi, Anitha, Arumugam, Paramasivam, Priyadharsini Jayaseelan, Vijayashree. MicroRNAs targeting CDKN2A gene as a potential prognostic marker in head and neck squamous cell carcinoma. , 1402; 13(1): 21-27. doi: 10.22099/mbrc.2023.48081.1853

MicroRNAs targeting CDKN2A gene as a potential prognostic marker in head and neck squamous cell carcinoma

Molecular Biology Research Communications
دوره 13، شماره 1، 2024، صفحه 21-27    اصل مقاله (222.19 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2023.48081.1853
نویسندگان
Sivakumar Gopalakrishnan1؛ Anitha Pandi1؛ Paramasivam Arumugam2؛ Vijayashree Priyadharsini Jayaseelan* 1
1Clinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College & Hospital, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Chennai, India
2Molecular Biology Lab, Centre for Cellular and Molecular Research, Saveetha Dental College & Hospital, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Chennai, India
چکیده
Epigenetic factors are known to markedly influence the functions of a gene by modification of transcripts, via methylation or acetylation and degradation of mRNA transcripts. The CDKN2A encodes cyclin-dependent kinase inhibitor 2A, a tumour suppressor protein. Genetic and epigenetic alterations in this gene have been demonstrated in several cancer types. The non-coding RNAs with a special emphasis on microRNAs have long been explored for their potential role in the epigenetic modification of gene expression. The present study aims to identify the microRNAs targeting CDKN2A gene transcripts and demonstrate their prognostic significance in head and neck squamous cell carcinoma (HNSCC). Computational approaches were employed to identify the microRNAs targeting CDKN2A. The gene and protein expression profile of CDKN2A was analyzed using UALCAN. A significant upregulation of CDKN2A was observed in the primary tumour tissues (p=<10-12). Interestingly, the protein expression, although found to be statistically significant (p=0.0129) did not correlate well with the gene expression profile. The microRNAs targeting CDKN2A were further analyzed to identify the possible reason for the decrease in protein expression. Among the 44 microRNAs targeting CDKN2A gene transcripts, hsa-miR-3681-3p, hsa-miR-542-5p, hsa-miR-4519 were found to be upregulated and hsa-miR-134-5p was found to be downregulated with a significant association with survival status of HNSCC patients. The hsa-miR-542-5p was found to correlate well with the survival and hence can be considered as the key microRNA associated with HNSCC. However, further validation of this microRNA is warranted to confirm its role in the process of carcinogenesis.
کلیدواژه‌ها
Cancer؛ Gene expression؛ microRNA؛ HNSCC؛ Survival؛ Novel biomarkers
مراجع
  1. Marret G, Bièche I, Dupain C, Borcoman E, du Rusquec P, Ricci F, Hescot S, Sablin MP, Tresca P, Bello D, Dubot C, Loirat D, Frelaut M, Lecerf C, Le Tourneau C, Kamal M. Genomic alterations in head and neck squamous cell carcinoma: Level of evidence according to ESMO scale for clinical actionability of molecular targets (ESCAT). JCO Precis Oncol 2021;5:215-226.
  2. Campbell BR, Chen Z, Faden DL, Agrawal N, Li RJ, Hanna GJ, Iyer NG, Boot A, Rozen SG, Vettore AL, Panda B, Krishnan NM, Pickering CR, Myers JN, Guo X, Lang Kuhs KA. The mutational landscape of early- and typical-onset oral tongue squamous cell carcinoma. Cancer 2021;127:544-553.
  3. Gupta B, Bray F, Kumar N, Johnson NW. Associations between oral hygiene habits, diet, tobacco and alcohol and risk of oral cancer: A case-control study from India. Cancer Epidemiol 2017;51:7-14.
  4. Kim S, Lee C, Kim H, Yoon SO. Genetic characteristics of advanced oral tongue squamous cell carcinoma in young patients. Oral Oncol 2023;144:106466.
  5. Chan SH, Chiang J, Ngeow J. CDKN2A germline alterations and the relevance of genotype-phenotype associations in cancer predisposition. Hered Cancer Clin Pract 2021;19:21.
  6. Goel H, Mathur R, Syeda S, Shrivastava A, Jha AK. Promoter hypermethylation of LATS1 fene in oral squamous cell carcinoma (OSCC) among North Indian population. Asian Pac J Cancer Prev 2021;22:977-982. 
  7. Liu Y, Tong X, Hu W, Chen D. HDAC11: A novel target for improved cancer therapy. Biomed Pharmacother 2023;166:115418.
  8. Dioguardi M, Spirito F, Iacovelli G, Sovereto D, Laneve E, Laino L, Caloro GA, Nabi AQ, Ballini A, Lo Muzio L, Troiano G. The potential microRNA Prognostic Signature in HNSCCs: A Systematic Review. Noncoding RNA 2023;9:54.
  9. Vahabi M, Blandino G, Di Agostino S. MicroRNAs in head and neck squamous cell carcinoma: a possible challenge as biomarkers, determinants for the choice of therapy and targets for personalized molecular therapies. Transl Cancer Res 2021;10:3090-3110.
  10. Cheng CJ, Bahal R, Babar IA, Pincus Z, Barrera F, Liu C, Svoronos A, Braddock DT, Glazer PM, Engelman DM, Saltzman WM, Slack FJ. MicroRNA silencing for cancer therapy targeted to the tumour microenvironment. Nature 2015;518:107-110.
  11. Peng Y, Croce CM. The role of MicroRNAs in human cancer. Signal Transduct Target Ther 2016;1:15004.
  12. Allen B, Schneider A, Victoria B, Nunez Lopez YO, Muller M, Szewczyk M, Pazdrowski J, Majchrzak E, Barczak W, Golusinski W, Golusinski P, Masternak MM. Blood serum from head and neck squamous cell carcinoma patients induces altered microRNA and target gene expression profile in treated cells. Front Oncol 2018;8:217.
  13. Bhattacharjee B, Syeda AF, Rynjah D, Hussain SM, Chandra Bora S, Pegu P, Sahu RK, Khan J. Pharmacological impact of microRNAs in head and neck squamous cell carcinoma: Prevailing insights on molecular pathways, diagnosis, and nanomedicine treatment. Front Pharmacol 2023;14:1174330.
  14. Dhuri K, Gaddam RR, Vikram A, Slack FJ, Bahal R. Therapeutic potential of chemically modified, synthetic, triplex peptide nucleic acid-based oncomir inhibitors for cancer therapy. Cancer Res 2021;81:5613-5624. 
  15. Nana-Sinkam SP, Croce CM. MicroRNA regulation of tumorigenesis, cancer progression and interpatient heterogeneity: towards clinical use. Genome Biol 2014;15:445. 
  16. Wong N, Wang X. miRDB: an online resource for microRNA target prediction and functional annotations. Nucleic Acids Res 2015;43(Database issue):D146-D152.
  17. Chen Y, Wang X. miRDB: an online database for prediction of functional microRNA targets. Nucleic Acids Res 2020;48:D127-D131.
  18. Chandrashekar DS, Karthikeyan SK, Korla PK, Patel H, Shovon AR, Athar M, Netto GJ, Qin ZS, Kumar S, Manne U, Creighton CJ, Varambally S. UALCAN: An update to the integrated cancer data analysis platform. Neoplasia 2022;25:18-27. 
  19. Feng J, Hsu PF, Esteva E, Labella R, Wang Y, Khodadadi-Jamayran A, Pucella J, Liu CZ, Arbini AA, Tsirigos A, Kousteni S, Reizis B. Haplodeficiency of the 9p21 tumour suppressor locus causes myeloid disorders driven by the bone marrow microenvironment. Blood 2023;142:460-476. 
  20. Shima K, Nosho K, Baba Y, Cantor M, Meyerhardt JA, Giovannucci EL, Fuchs CS, Ogino S. Prognostic significance of CDKN2A (p16) promoter methylation and loss of expression in 902 colorectal cancers: Cohort study and literature review. Int J Cancer 2011;128:1080-1094.
  21. Chen Z, Guo Y, Zhao D, Zou Q, Yu F, Zhang L, Xu L. Comprehensive analysis revealed that CDKN2Ais a biomarker for immune infiltrates in multiple cancers. Front Cell Dev Biol 2021;9:808208.
  22. Aditya J, Smiline Girija AS, Paramasivam A, Vijayashree Priyadharsini J. Genetic alterations in Wnt family of genes and their putative association with head and neck squamous cell carcinoma. Genomics Inform 2021;19:e5.
  23. Fathima T, Arumugam P, Girija As S, Priyadharsini JV. Decoding the genetic alterations in genes of DNMT family (DNA Methyl-Transferase) and their association with head and neck squamous cell carcinoma. Asian Pac J Cancer Prev 2020;21:3605-3612.
  24. Jayaseelan VP, Ramesh A, Arumugam P. Breast cancer and DDT: putative interactions, associated gene alterations, and molecular pathways. Environ Sci Pollut Res Int 2021;28: 27162-27173.
  25. Anita R, Paramasivam A, Priyadharsini JV, Chitra S. The m6A readersYTHDF1 and  YTHDF3 aberrations associated with metastasis and predict poor prognosis in breast cancer patients. Am J Cancer Res 2020;10:2546-2554. 
  26. Paramasivam A, George R, Priyadharsini JV. Genomic and transcriptomic alterations in m6A regulatory genes are associated with tumorigenesis and poor prognosis in head and neck squamous cell carcinoma. Am J Cancer Res 2021;11:3688-3697.
  27. Jayaseelan VP, Arumugam P. Exosomal microRNAs targeting TP53 gene as promising prognostic markers for head and neck squamous cell carcinoma. Glob Med Genet 2022;9: 277-286.
  28. Wu N, Lu Y, Liang JZ. Expression and correlation of survivin and hsa-miR-542-3p in patients with oral squamous cell carcinoma. Shanghai Kou Qiang Yi Xue 2016;25:720-724. [Chinese]
  29. Patel S, Rawal R. Role of miRNA dynamics and cytokine profile in governing CD44v6/Nanog/PTEN axis in oral cancer: modulating the master regulators. Tumour Biol 2016;37:14565-14575.
  30. Cheng DD, Yu T, Hu T, Yao M, Fan CY, Yang QC. MiR-542-5p is a negative prognostic factor and promotes osteosarcoma tumorigenesis by targeting HUWE1. Oncotarget 2015; 6:42761-42772.
  31. Silva CMS, Barros-Filho MC, Wong DVT, Mello JBH, Nobre LMS, Wanderley CWS, Lucetti LT, Muniz HA, Paiva IKD, Kuasne H, Ferrira DPP, Cunha MPSS, Hirth CG, Silva PGB, Sant'Ana RO, Souza MHLP, Quetz JS, Rogatoo SR, Lima-Junior RCP. Circulating let-7e-5p, miR-106a-5p, miR-28-3p, and miR-542-5p as a promising microRNA signature for the detection of colorectal cancer. Cancers (Basel) 2021;13:1493.
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