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Adelipour, Maryam, Naghashpour, Mahshid, Roshanazadeh, Mohammad Reza, Chenaneh, Hadi, Mohammadi, Asma, Pourangi, Pegah, Miri, Seyed Rouhollah, Zahedi, Atefeh, Haghighatnezhad, Mahmood, Golabi, Sahar. (1402). Evaluation of Beclin1 and mTOR genes and p62 protein expression in breast tumor tissues of Iranian patients. , 13(1), 11-19. doi: 10.22099/mbrc.2023.47597.1837
Maryam Adelipour; Mahshid Naghashpour; Mohammad Reza Roshanazadeh; Hadi Chenaneh; Asma Mohammadi; Pegah Pourangi; Seyed Rouhollah Miri; Atefeh Zahedi; Mahmood Haghighatnezhad; Sahar Golabi. "Evaluation of Beclin1 and mTOR genes and p62 protein expression in breast tumor tissues of Iranian patients". , 13, 1, 1402, 11-19. doi: 10.22099/mbrc.2023.47597.1837
Adelipour, Maryam, Naghashpour, Mahshid, Roshanazadeh, Mohammad Reza, Chenaneh, Hadi, Mohammadi, Asma, Pourangi, Pegah, Miri, Seyed Rouhollah, Zahedi, Atefeh, Haghighatnezhad, Mahmood, Golabi, Sahar. (1402). 'Evaluation of Beclin1 and mTOR genes and p62 protein expression in breast tumor tissues of Iranian patients', , 13(1), pp. 11-19. doi: 10.22099/mbrc.2023.47597.1837
Adelipour, Maryam, Naghashpour, Mahshid, Roshanazadeh, Mohammad Reza, Chenaneh, Hadi, Mohammadi, Asma, Pourangi, Pegah, Miri, Seyed Rouhollah, Zahedi, Atefeh, Haghighatnezhad, Mahmood, Golabi, Sahar. Evaluation of Beclin1 and mTOR genes and p62 protein expression in breast tumor tissues of Iranian patients. , 1402; 13(1): 11-19. doi: 10.22099/mbrc.2023.47597.1837

Evaluation of Beclin1 and mTOR genes and p62 protein expression in breast tumor tissues of Iranian patients

Molecular Biology Research Communications
دوره 13، شماره 1، 2024، صفحه 11-19    اصل مقاله (366.22 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2023.47597.1837
نویسندگان
Maryam Adelipour1؛ Mahshid Naghashpour2؛ Mohammad Reza Roshanazadeh1؛ Hadi Chenaneh1؛ Asma Mohammadi3؛ Pegah Pourangi3؛ Seyed Rouhollah Miri4؛ Atefeh Zahedi5؛ Mahmood Haghighatnezhad3؛ Sahar Golabi* 6
1Department of Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2Department of Nutrition, Shool of Medicine, Abadan University of Medical Science, Abadan, Iran
3Department of Biochemistry, Shool of Medicine, Abadan University of Medical Science, Abadan, Iran
4Department of surgical oncology, Cancer institute, Tehran University of Medical Science
5Asadabad School of Medical Sciences, Asadabad, Iran
6Department of Physiology, Shool of Medicine, Abadan University of Medical Science, Abadan, Iran
چکیده
Autophagy is a cellular process that plays a major role in the fate of tumor cells. Understanding the role of autophagy in cancer therapy is a major challenge, particularly for breast cancer as the sole top cause of mortality among women. In this study, we evaluated the gene expression of mTOR and Beclin1 and the levels of p62 protein, in breast tumors and compared them to a control condition. To explore the role of autophagy in breast cancer, we acquired tumor biopsies from 41 new cases of breast cancer patients. We extracted total RNA from each biopsy and used real-time PCR to quantify Beclin1 and mTOR-specific RNA expression. In addition, we evaluated the expression of the p62 protein in paraffin-embedded tumor tissue using the immunohistochemistry technique. The data revealed an upregulation of Beclin1 and a downregulation of mTOR in tumor tissues compared to the control condition. The correlation between p62 expression and Beclin1/mTOR showed a negative and positive correlation, respectively, confirming autophagy activation in the tumor tissues. However, there was no correlation between autophagy markers and tumor size, grade and stage. The findings revealed that autophagy activation was found in breast tumor tissues, suggesting that autophagy can be a target for breast cancer therapy.
کلیدواژه‌ها
Autophagy؛ Beclin-1؛ Breast cancer؛ mTOR؛ Tumor grade
مراجع
  1. Yoshii SR, Mizushima N. Monitoring and measuring autophagy. Int J Mol Sci 2017;18:1865.
  2. Kaushik S, Bandyopadhyay U, Sridhar S, Kiffin R, Martinez-Vicente M, Kon M, Orenstein SJ, Wong E, Cuervo AM. Chaperone-mediated autophagy at a glance. J Cell Sci 2011;124: 495-499.
  3. Cuervo AM, Wong E. Chaperone-mediated autophagy: roles in disease and aging. Cell Res 2014; 24:92-104.
  4. Schuck S. Microautophagy–distinct molecular mechanisms handle cargoes of many sizes. J Cell Sci 2020;133:jcs246322.
  5. He C, Klionsky DJ. Regulation mechanisms and signaling pathways of autophagy. Annu Rev Genet 2009;43:67-93.
  6. Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research. Cell 2010; 140:313-326.
  7. Berardi DE, Campodónico PB, Díaz Bessone MI, Urtreger AJ, Todaro LB. Autophagy: friend or foe in breast cancer development, progression, and treatment. Int J Breast Cancer 2011; 2011:595092.
  8. Rabanal-Ruiz Y, Otten EG, Korolchuk VI. mTORC1 as the main gateway to autophagy. Essays Biochem 2017;61:565-584.
  9. Saxton RA, Sabatini DM. mTOR signaling in growth, metabolism, and disease. Cell 2017;168:960-976.
  10. Paquette M, El-Houjeiri L, Pause A. mTOR pathways in cancer and autophagy. Cancers(Basel) 2018;10:18.
  11. Chen N, Karantza V. Autophagy as a therapeutic target in cancer. Cancer Biol Ther 2011; 11:157-168.
  12. White E. The role for autophagy in cancer. J Clin Invest 2015;125:42-46.
  13. Levy JMM, Towers CG, Thorburn A. Targeting autophagy in cancer. Nat Rev Cancer 2017; 17:528-542.
  14. Li X, He S, Ma B. Autophagy and autophagy-related proteins in cancer. Mol Cancer 2020; 19:12.
  15. Sun K, Deng W, Zhang S, Cai N, Jiao S, Song J, Wei L. Paradoxical roles of autophagy in different stages of tumorigenesis: protector for normal or cancer cells. Cell Biosci 2013; 3:35.
  16. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2018;68:394-424.
  17. Akram M, Iqbal M, Daniyal M, Khan AU. Awareness and current knowledge of breast cancer. Biol Res 2017;50:33.
  18. Nakayama S, Karasawa H, Suzuki T, Yabuuchi S, Takagi K, Aizawa T, Onodera Y, Nakamura Y, Watanabe M, Fujishima F, Yoshida H, Morikawa T, Sase T, Naitoh T, Unno M, Sasano H. p62/sequestosome 1 in human colorectal carcinoma as a potent prognostic predictor associated with cell proliferation. Cancer Med 2017;6:1264-1274.
  19. Arai A, Chano T, Ikebuchi K, Hama Y, Ochi Y, Tameno H, Shimada T. p62/SQSTM1 levels predicts radiotherapy resistance in hypopharyngeal carcinomas. Am J Cancer Res 2017;7:881-891.
  20. Berardi DE, Campodónico PB, Díaz Bessone MI, Urtreger AJ, Todaro LB. Autophagy: friend or foe in breast cancer development, progression, and treatment. Int J Breast Cancer 2011;2011.
  21. Flynn ALB, Schiemann WP. Autophagy in breast cancer metastatic dormancy: Tumor suppressing or tumor promoting functions?. J Cancer Metastasis Treat 2019;5:43.
  22. Maycotte P, Thorburn A. Targeting autophagy in breast cancer. World J Clin Oncol 2014; 5:224-240.
  23. Han Y, Fan S, Qin T, Yang J, Sun Y, Lu Y, Mao J, Li L. Role of autophagy in breast cancer and breast cancer stem cells. Int J Oncol 2018;52:1057-1070.
  24. Hu YL, DeLay M, Jahangiri A, Molinaro AM, Rose SD, Carbonell WS, Aghi MK. Hypoxia-induced autophagy promotes tumor cell survival and adaptation to antiangiogenic treatment in glioblastoma. Cancer Res 2012;72:1773-1783.
  25. Vera-Ramirez L, Vodnala SK, Nini R, Hunter KW, Green JE. Autophagy promotes the survival of dormant breast cancer cells and metastatic tumour recurrence. Nat Commun 2018;9:1944.
  26. Yun CW, Lee SH. The roles of autophagy in cancer. Int J Mol Sci 2018;19:3466.
  27. Chen W, Bai Y, Patel C, Geng F. Autophagy promotes triple negative breast cancer metastasis via YAP nuclear localization. Biochem Biophys Res Commun 2019;520:263-268.
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