| تعداد نشریات | 24 |
| تعداد شمارهها | 877 |
| تعداد مقالات | 7,848 |
| تعداد مشاهده مقاله | 14,537,457 |
| تعداد دریافت فایل اصل مقاله | 12,272,590 |
The examination of some virulence factors in S. aureus isolates obtained from the healthy human population, sheep mastitis, and cheese | ||
| Iranian Journal of Veterinary Research | ||
| دوره 24، شماره 2 - شماره پیاپی 83، 2023، صفحه 110-115 اصل مقاله (358.75 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2023.43730.6410 | ||
| نویسندگان | ||
| L. Rahbarnia1؛ R. Khosravi Rad2؛ AR. Dehnad3؛ B. Naghili1 | ||
| 1Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2MSc in Microbiology, Higher Education Institute of Rab-Rashid, Tabriz, Iran | ||
| 3Department of Livestock Bacterial Diseases Research, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| Background: Staphylococcus aureus is responsible for many infections in humans and animals from skin and soft tissue infections to life-threatening diseases. In this study to explore the origin of S. aureus infections in humans, the antibiotic resistance profile and the variety of virulence factors in S. aureus isolates were examined in three groups: a healthy human population, cheese, and the milk of sheep with mastitis. Aims: The examination of some virulence factors in S. aureus isolates obtained from the healthy human population, sheep mastitis, and cheese. Methods: A total of 400 nasal swab samples from healthy students, 30 cheese samples, and 122 sheep milk samples were collected for the detection of S. aureus isolates from January 1, 2018, to March 1, 2018. The frequency of hla, hlb, Acme/arcA, pvl, and tsst-1 virulence genes and mecA gene was determined in each group by PCR assay. Results: There was a direct relationship between the antibiotic susceptibility profile of the isolates from a healthy population and those from mastitis milk samples. Of 400 nasal samples, 15% (60/400) were positive for S. aureus, of which 60% (36/60) were positive for mecA. While 50% (15/30) of cheese samples were positive for S. aureus. of which 7 cases (46.66%, 7/15) were positive for mecA. The prevalence of S. aureus among students was dependent on gender (P=0.025). Also, 47.5% (58/122) of milk samples from sheep mastitis were positive for S. aureus, and 41.37% (24/58) were positive for the mecA gene. Based on PCR results, the highest rate of hla (68.33%, 41/60), hlb (53.33%, 32/60), and Acme/arcA (46.66%, 28/60) genes were related to a healthy population, and the highest frequency of pvl (41.38%, 24/58), and tsst-1 (27.59%, 16/58) was related to milk samples (P<0.05). A significant correlation was observed between the presence of the arginine catabolic mobile element (ACME)-arcA gene and resistance to methicillin (P<0.05). Conclusion: The high rate of virulence factors in the S. aureus isolates obtained from mastitis and dairy products is an alert point, because they could be source of the spreading of S. aureus to humans. There is an essential need for continuous monitoring to control staphylococcal food poisoning. | ||
| کلیدواژهها | ||
| Dairy products؛ Healthy population؛ Mastitis؛ S. aureus؛ Virulence factors | ||
| مراجع | ||
|
Aghamohammadi, M; Haine, D; Kelton, DF; Barkema, HW; Hogeveen, H; Keefe, GP and Dufour, S (2018). Herd-level mastitis-associated costs on Canadian dairy farms. Front. Vet. Sci., 5: 1-12.
Anderson, H; Hinds, P; Hurditt, S; Miller, P; McGrowder, D and Alexander-Lindo, R (2011). The microbial content of unexpired pasteurized milk from selected supermarkets in a developing country. Pac. J. Trop. Biomed., 1: 205-211.
Castro, R; Pedroso, S; Sandes, S; Silva, G; Luiz, K; Dias, R; Figueiredo, H; Santos, S; Nunes, A and Souza, M (2020). Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from the production process of Minas artisanal cheese from the region of Campo das Vertentes, Brazil. J. Dairy Sci., 103: 2098-2110.
Dastmalchi, SH; Aghdasi, S and Mohammad, ZH (2013). Survey of accessory gene regulator (agr) groups and TSST-1 encoding gene (tst) in Staphylococcus aureus isolated from ewes with mastitis in the northwest of Iran. Iran. J. Vet. Res., 14: 334-340.
Dastmalchi Saei, H and Panahi, M (2020). Genotyping and antimicrobial resistance of Staphylococcus aureus isolates from dairy ruminants: differences in the distribution of clonal types between cattle and small ruminants. Arch. Microbiol., 202: 115-125.
Dehnad, A; Agdam, MHG; Rahbarnia, L; Naghili, B and Saffarian, P (2020). Detection of hemolysine genes in methicillin-resistant S. aureus isolates obtained from a healthy population in north-west of Iran. Gene Rep., 21: 100874.
den Heijer, CD; van Bijnen, EM; Paget, WJ; Pringle, M; Goossens, H; Bruggeman, CA; Schellevis, FG; Stobberingh, EE and Team, AS (2013). Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study. Lancet Infect. Dis., 13: 409-415.
Ebrahimi, A; Soleimani, F; Motamedi, A; Shams, N and Lotfalian, S (2014). Study on some characteristics of Staphylococci isolated from sheep sub clinical mastitis milk in Shahrekord, Iran. B.J.M., 2: 57-62.
Fard-Mousavi, N; Mosayebi, G; Amouzandeh-Nobaveh, A; Japouni-Nejad, A and Ghaznavi-Rad, E (2015). The dynamic of Staphylococcus aureus nasal carriage in Central Iran. Jundishapur J. Microbiol., 8: e20760.
Fluit, A (2012). Livestock-associated Staphylococcus aureus. Clin. Microbiol. Infect., 18: 735-744.
Garipcin, M and Seker, E (2015). Nasal carriage of methicillin-resistant Staphylococcus aureus in cattle and farm workers in Turkey. Vet. Arh., 85: 117-129.
Garoy, EY; Gebreab, YB; Achila, OO; Tekeste, DG; Kesete, R; Ghirmay, R; Kiflay, R and Tesfu, T (2019). Methicillin-resistant Staphylococcus aureus (MRSA): prevalence and antimicrobial sensitivity pattern among patients—a multicenter study in Asmara, Eritrea. Can. J. Infect. Dis. Med. Microbiol., 2019: 1-9.
Ghavghani, FR; Rahbarnia, L; Naghili, B; Dehnad, A; Bazmani, A; Varshochi, M and Agdam, MHG (2019). Nasal and extra nasal MRSA colonization in hemodialysis patients of north-west of Iran. BMC Res. Notes. 12: 1-5.
Hoppe, PA; Hanitsch, LG; Leistner, R; Niebank, M; Bührer, C; von Bernuth, H and Krüger, R (2018). Periorbital infections and conjunctivitis due to Panton-Valentine Leukocidin (PVL) positive Staphylococcus aureus in children. BMC Infect. Dis., 18: 371-375.
Humphreys, H; Fitzpatick, F and Harvey, BJ (2015). Gender differences in rates of carriage and bloodstream infection caused by methicillin-resistant Staphylococcus aureus: are they real, do they matter and why? Arch. Clin. Infect. Dis., 61: 1708-1714.
Ito, T; Okuma, K; Ma, XX; Yuzawa, H and Hiramatsu, K (2003). Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC. Drug. Resist. Updat., 6: 41-52.
Japoni-Nejad, A; Rezazadeh, M; Kazemian, H; Fardmousavi, N; van Belkum, A and Ghaznavi-Rad, E (2013). Molecular characterization of the first community-acquired methicillin-resistant Staphylococcus aureus strains from Central Iran. Int. J. Infect. Dis., 17: 949-954.
Koymans, KJ; Vrieling, M; Gorham, RD and van Strijp, JA (2015). Staphylococcal immune evasion proteins: structure, function, and host adaptation. Curr. Top. Microbiol. Immunol., 409: 441-489.
Kwiatkowski, P; Masiuk, H; Pruss, A; Łopusiewicz, Ł; Sienkiewicz, M; Wojciechowska-Koszko, I; Roszkowska, P; Bania, J; Guenther, S and Dołęgowska, B (2022). Clonal diversity, antimicrobial susceptibility and presence of genes encoding virulence factors in Staphylococcus aureus strains isolated from cut wound infections. Curr. Microbiol., 79: 1-11.
Lindsay, JA and Holden, MT (2004). Staphylococcus aureus: superbug, super genome? Trends. Microbiol., 12: 378-385.
Magro, G; Biffani, S; Minozzi, G; Ehricht, R; Monecke, S; Luini, M and Piccinini, R (2017). Virulence genes of S. aureus from dairy cow mastitis and contagiousness risk. Toxins. 9: 195.
Mistry, H; Sharma, P; Mahato, S; Saravanan, R; Kumar, PA and Bhandari, V (2016). Prevalence and characterization of oxacillin susceptible mecA-positive clinical isolates of Staphylococcus aureus causing bovine mastitis in India. PLoS One. 11: e0162256.
Monistero, V; Graber, HU; Pollera, C; Cremonesi, P; Castiglioni, B; Bottini, E; Ceballos-Marquez, A; Lasso-Rojas, L; Kroemker, V and Wente, N (2018). Staphylococcus aureus isolates from bovine mastitis in eight countries: genotypes, detection of genes encoding different toxins and other virulence genes. Toxins. 10: 247.
Motamedi, H; Abadi, SSR; Moosavian, SM and Torabi, M (2015). The association of Panton-valentine leukocidin and mecA genes in methicillin-resistant staphylococcus aureus isolates from patients referred to educational hospitals in Ahvaz, Iran. Jundishapur J. Microbiol., 8: e22021.
Niemann, S; Bertling, A; Brodde, MF; Fender, AC; Van de Vyver, H; Hussain, M; Holzinger, D; Reinhardt, D; Peters, G and Heilmann, C (2018). Panton-Valentine Leukocidin associated with S. aureus osteomyelitis activates platelets via neutrophil secretion products. Sci. Rep., 8: 1-15.
Peton, V and Le Loir, Y (2014). Staphylococcus aureus in veterinary medicine. Infect. Genet. Evol., 21: 602-615. Sabat, AJ; Ilczyszyn, WM; van Rijen, M; Akkerboom, V; Sinha, B; Kluytmans, J; Miedzobrodzki, J; Grundmann, H and Friedrich, AW (2015). Genome-wide analysis reveals two novel mosaic regions containing an ACME with an identical DNA sequence in the MRSA ST398-t011 and MSSA ST8-t008 isolates. J. Antimicrob. Chemother., 70: 1298-1302.
Szczuka, E; Porada, K; Wesołowska, M and Łęska, B (2022). Occurrence and characteristics of Staphylococcus aureus isolated from dairy products. Molecules. 27: 4649.
Thurlow, LR; Joshi, GS; Clark, JR; Spontak, JS; Neely, CJ; Maile, R and Richardson, AR (2013). Functional modularity of the arginine catabolic mobile element contributes to the success of USA300 methicillin-resistant Staphylococcus aureus. Cell Host Microbe. 13: 100-107.
Ünal, N; Askar, Ş; Macun, HC; Sakarya, F; Altun, B and Yıldırım, M (2012). Panton-Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants. Trop. Anim. Health Prod., 44: 573-579.
Wang, W; Lin, X; Jiang, T; Peng, Z; Xu, J; Yi, L; Li, F; Fanning, S and Baloch, Z (2018). Prevalence and characterization of Staphylococcus aureus cultured from raw milk taken from dairy cows with mastitis in Beijing, China. Front. Microbiol., 9: 1123.
Weinstein, MP and Lewis II, JS (2020). The clinical and laboratory standards institute subcommittee on anti-microbial susceptibility testing: background, organization, functions, and processes. J. Clin. Microbiol., 58: e01864-19.
Xiao, M; Zhao, R; Zhang, Q; Fan, X; O’Sullivan, MV; Li, DF; Wang, XY; Wu, HL; Kong, F and Xu, YC (2016). Genotypic diversity of Staphylococcus aureus α-hemolysin gene (hla) and its association with clonal background: implications for vaccine development. PLoS One. 11: e0149112.
Zeinalpour Ahrabi, S; Rahbarnia, L; Dehnad, A; Naghili, B; Ghaffari Agdam, MH and Nazari, A (2019). Incidence of oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) isolates and TSST-1 virulence factor among high school students in Tabriz, Northwest of Iran. Arch. Clin. Infect. Dis., 14: e85341. | ||
|
آمار تعداد مشاهده مقاله: 642 تعداد دریافت فایل اصل مقاله: 555 |
||